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Bioflorida Chapter Meeting Jupiter 2008v2

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EcoArray provides various services related to gene discovery, microarray development, and bioinformatics including: 1) cDNA library construction for identifying expressed genes and differentially expressed genes between populations. 2) Custom gene array construction and hybridizations for species without commercially available arrays. 3) RT-PCR for validating gene expression changes between controls and treated samples. 4) Bioinformatics services such as gene sequencing, annotation, and statistical analysis of microarray data.

Technology
1 of 14
Expertise in Gene Discovery, Microarray Development and Bioinformatics Heather R. Hammers BioFlorida’s Southeast Chapter Meeting Jupiter, Florida September 23, 2008
Business is Expanding ,[object Object],[object Object]
DNA Precision- Our Services ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Library Construction Other Services
Library Construction
Create cDNA libraries ; Identify all expressed genes within a tissue Create subtraction libraries To identify differentially Expressed genes between two populations CLONE GENES AND SEQUENCE ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Custom Gene Array Construction “Microarrays”
You are interested in microarray technology but… ,[object Object],[object Object],Species of interest not yet sequenced? No commercially available array for your model organism?
Hybridizations (Array Processing in our Lab) Agilent Microarray Kits 1. Microarray-Based Gene Expression Analysis One-Color Quick-AMP Labeling Kit (#5190-0442) Two Color Quick-Amp Labeling Kit (#5190-0444) 2. RNA Spike-In Kits One Color (#5188-5282) Two Color (#5188-5279) 3. Gene Expression Hybridization Kits (#5188-5242) 4. Gene Expression Wash Buffers 1 and 2 5. Stabilization and Drying Solution
RT-PCR
[object Object],[object Object],[object Object]
Bioinformatics
Gene Sequencing and Annotation ,[object Object],[object Object],[object Object],“ Let DNA Precision submit your clones to our sequencing facility ”
Statistical Analysis Now what? We can do the analysis for you . Advanced Basic ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],(optional) ,[object Object],[object Object],[object Object],[object Object]
Expertise in Gene Discovery, Microarray Development and Bioinformatics EcoArray, Inc. www.ecoarray.com Heather R. Hammers (Bioinformatician/Scientist) [email_address]

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Bioflorida Chapter Meeting Jupiter 2008v2

  • 1. Expertise in Gene Discovery, Microarray Development and Bioinformatics Heather R. Hammers BioFlorida’s Southeast Chapter Meeting Jupiter, Florida September 23, 2008
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  • 6. Custom Gene Array Construction “Microarrays”
  • 7.
  • 8. Hybridizations (Array Processing in our Lab) Agilent Microarray Kits 1. Microarray-Based Gene Expression Analysis One-Color Quick-AMP Labeling Kit (#5190-0442) Two Color Quick-Amp Labeling Kit (#5190-0444) 2. RNA Spike-In Kits One Color (#5188-5282) Two Color (#5188-5279) 3. Gene Expression Hybridization Kits (#5188-5242) 4. Gene Expression Wash Buffers 1 and 2 5. Stabilization and Drying Solution
  • 10.
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  • 14. Expertise in Gene Discovery, Microarray Development and Bioinformatics EcoArray, Inc. www.ecoarray.com Heather R. Hammers (Bioinformatician/Scientist) [email_address]