1. Using Proteomics to
Understand Crassostrea
gigas’s Response to
Multiple Stressors
Emma Timmins-Schiffman (UW, SAFS)
Brook Nunn (UW, Med. Chem.)
David Goodlett (UW, Med. Chem.)
Carolyn Friedman (UW, SAFS)
Steven Roberts (UW, SAFS)
8. Experimental Design
• Mechanical stress as a proxy for environmental
stress
• MS promotes a catecholmine stress response in C.
gigas (Lacoste et al. 2001)
Proteins?
21. Summary
Exposure to multiple stressors can impact an
organism’s ability to mount a successful response to
either stressor.
22. Implications
We should continue to consider multiple stressors when
assessing responses to environmental change.
0.02
LowMS2
HighMS1
0.01
High1
High4
HighMS4
LowMS4
HighMS2
0.00
Axis 2
LowMS3 HighMS3
High2
High3 LowMS1
Low4
Low3
-0.01
Low2
Low1
-0.02
-0.02 -0.01 0.00 0.01 0.02 0.03 0.04
Axis 1
23. Acknowledgements
• Taylor Shellfish: Joth Davis, Jason Ragan, Dustin
Johnson
• Friday Harbor Labs: Emily Carrington, Ken Sebens,
Michael O’Donnell, Matt George, Michelle Herko
• UW SAFS: Sam White, Mackenzie Gavery, Caroline
Storer, Samantha Brombacker, Lisa Crosson, Julian
Olden
• UW Proteomics Resource: Priska von Haller, Jimmy
Eng, Eva Jahan
• Ronen Elad, Sam Garson, Chris Parrish
• Funding: NOAA Saltonstall-Kennedy, RocketHub