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Presented at an internal seminar held in Plant Breeding Laboratory Universitas Brawijaya, Malang, 8 December 2015
ADDITIVE MAIN EFFECTS AND
MULTIPLICATIVE INTERACTION (AMMI)
BIPLOT ANALYSIS FOR MATCHING LOCAL
VARIETIES SWEETPOTATO YIELD ON TO
CLIMATE CHANGE CHALLANGE
Budi Waluyo
contact : budiwaluyo@ub.ac.id
UNIVERSITAS BRAWIJAYA - FACULTY OF AGRICULTURE
LABORATORY OF PLANT BREEDING
 Sweet potato is an potential comodity for
food, feed, industry, and bioethanol raw
material
 Targeting sweetpotato local varieties
selection onto environmental change is the
interest of any plant-breeding program to
matching yield production
 AMMI biplot was simpler and informatif
geometry for producing graphical
visualizations of patterns in yield-trial
data, can capture substantially more
variation.
 AMMI biplot displays genotype (G) main
effects, environment (E) main effects, and
genotype × environment (GE) interaction
eff ects of interest to crop scientists and
soil scientists would need another biplot
Study of genotype x environment interactions and to obtain
local varieties widely adapted or adapted to specific
environment conducted at Kebun Percobaan Fakultas
Pertanian Universitas Padjadjaran. Period of research in
January 2011 - June 2011, July 2011 - December 2011, April
2012 - September 2012, July 2012 - December 2012, and
September 2012 – February 2013
A randomized complete block design with two replicates
over five times planting were employed with 33 local
varieties as treatments. Combined of Anova integrated with
AMMI was applied to detect the genotype x environment
interaction, to estimated genetic parameter, and determine
adaptation and yield stability.
Source df
Yield (t/ha)
MS (%) Total (%) GE
Season 4 1815.2** 32.0
Block(Season) 5 2.3
Varieties 32 268.91** 38.0
Varieties x Season 128 46.57** 26.3
Component AMMI 1 35 85.81** 50.4
Component AMMI 2 33 59.4** 32.9
Component AMMI 3 31 25.1** 13.0
Error 29 7.59 3.7
Residual 160 5.22 3.7
Total 329 68.92
KK (%) 26.61
Parameters
Trait
Yield (t/ha)
Var g 22.23
Var f 27.45
Var gm 20.68
Var e 5.22
KKG 54.9%
KKF 61.0%
KKGM 53.0%
SE var g 20.71
Criteria broad
SE var f 9.53
Criteria broad
H 0.81
Criteria high
Local Varieties Season 1 Season 2 Season 3 Season 4 Season 5 Average
Bagolo 2,8 d 2,3 b 9,2 d 10,2 e 7,6 d 6,4
Eno 8,5 c 5,9 a 4,8 d 24,1 c 15,6 c 11,8
Ganola 8,5 c 6,7 a 18,0 b 20,5 d 7,4 d 12,2
Jawer Kotok1 16,0 a 5,5 a 21,5 b 24,4 c 18,4 b 17,2
Jawer Kotok2 16,6 a 8,5 a 20,3 b 23,5 c 29,4 a 19,7
Menes Gedang 9,0 c 1,8 b 5,5 d 20,9 d 4,8 d 8,4
Menes01 9,0 c 2,4 b 7,0 d 17,2 d 8,6 d 8,8
Nirkum02 8,0 c 4,3 a 1,5 e 2,1 f 0,8 e 3,3
Nirkum03 7,0 c 4,5 a 5,3 d 13,1 e 3,6 e 6,7
Nirkum06 0,6 d 0,9 b 2,0 e 17,2 d 1,2 e 4,4
Nirkum08 4,6 d 3,0 b 2,2 e 7,2 f 8,8 d 5,2
Nirkum09 6,6 c 4,4 a 3,7 e 16,3 d 1,6 e 6,5
Nirkum12 10,5 b 2,2 b 6,8 d 18,6 d 2,4 e 8,1
Nirkum13 6,5 c 3,0 b 6,0 d 17,4 d 5,0 d 7,6
Nirkum14 1,7 d 1,7 b 0,2 e 14,8 e 1,1 e 3,9
Nirkum16 1,5 d 0,4 b 3,3 e 2,8 f 1,0 e 1,8
Nirkum18 10,0 b 1,5 b 3,0 e 22,1 c 2,8 e 7,9
Nirkum19 7,8 c 3,6 b 0,5 e 17,7 d 0,8 e 6,1
Nirkum20 6,8 c 3,2 b 7,7 d 11,6 e 5,4 d 6,9
Nirkum21 5,3 c 2,1 b 1,7 e 23,4 c 9,8 d 8,5
Nirkum22 10,0 b 4,9 a 0,6 e 35,2 b 4,6 d 11,1
Nirkum23 5,5 c 1,5 b 0,3 e 1,3 f 1,0 e 1,9
Nirkum24 4,5 d 2,7 b 2,0 e 18,2 d 1,8 e 5,8
Nirkum25 6,4 c 3,7 b 1,7 e 11,1 e 1,0 e 4,8
Nirkum26 8,5 c 2,1 b 8,0 d 13,2 e 1,4 e 6,6
Nirkum27 5,2 c 1,7 b 8,2 d 14,8 e 1,4 e 6,3
Menes NK 13,3 a 4,5 a 1,2 e 23,6 c 1,4 e 8,8
Nirkum29 5,6 c 0,2 b 9,0 d 4,1 f 2,2 e 4,2
Nirkum31 6,8 c 1,9 b 2,3 e 9,5 e 3,0 e 4,7
Odos 8,0 c 2,6 b 3,0 e 26,9 c 14,7 c 11,0
Rancing 13,5 a 6,4 a 28,0 a 48,2 a 26,0 a 24,4
Rancung01 11,5 b 9,3 a 13,0 c 20,9 d 20,6 b 15,1
Rancung02 11,2 b 6,7 a 19,3 b 24,7 c 25,0 a 17,4
Average 7,80 3,51 6,87 17,47 7,28 8,6
Tuber yield stable over planting 2011 – 2013 : Variety Menes 01, Nirkum 13, and
Nirkum 27
Jawer K-2
Menes01
Menes Ged
Nir-02
Nir-03
Nir-06
Rancung01
Rancing
Bagolo
Eno
Ganola
Nir-08
Nir-09
Nir-12
Nir-13
Nir-14
Jawer K-1
Nir-16
Nir-18
Nir-19
Nir-20Nir-21
Nir-22
Nir-23
Nir-24
Nir-25
Nir-26
Nir-27
Menes-NK
Nir-29
Nir-31
Odos
M1
M2
M3
M5
M4
-3.0
-2.0
-1.0
0.0
1.0
2.0
3.0
4.0
-6.0 -5.0 -4.0 -3.0 -2.0 -1.0 0.0 1.0 2.0
IPCA2-32.9%
IPCA1 - 50.4 %
AMMI Biplot GEI of Yield (t/ha)
 Yield of local varieties of sweet potatoes is
influenced by genotype x environment
interactions
 There are three local varieties that have a
phenotypicaly stable in tuber yield, i.e.
variety Menes 01, Nirkum13, and
Nirkum27
 THANK YOU
Sweetpotato
ice cream
Sweetpotato
mini stick
Roasted
Sweetpotato

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ADDITIVE MAIN EFFECTS AND MULTIPLICATIVE INTERACTION (AMMI) BIPLOT ANALYSIS FOR MATCHING LOCAL VARIETIES SWEETPOTATO YIELD ON TO CLIMATE CHANGE CHALLANGE

  • 1. Presented at an internal seminar held in Plant Breeding Laboratory Universitas Brawijaya, Malang, 8 December 2015 ADDITIVE MAIN EFFECTS AND MULTIPLICATIVE INTERACTION (AMMI) BIPLOT ANALYSIS FOR MATCHING LOCAL VARIETIES SWEETPOTATO YIELD ON TO CLIMATE CHANGE CHALLANGE Budi Waluyo contact : budiwaluyo@ub.ac.id UNIVERSITAS BRAWIJAYA - FACULTY OF AGRICULTURE LABORATORY OF PLANT BREEDING
  • 2.  Sweet potato is an potential comodity for food, feed, industry, and bioethanol raw material  Targeting sweetpotato local varieties selection onto environmental change is the interest of any plant-breeding program to matching yield production
  • 3.  AMMI biplot was simpler and informatif geometry for producing graphical visualizations of patterns in yield-trial data, can capture substantially more variation.  AMMI biplot displays genotype (G) main effects, environment (E) main effects, and genotype × environment (GE) interaction eff ects of interest to crop scientists and soil scientists would need another biplot
  • 4. Study of genotype x environment interactions and to obtain local varieties widely adapted or adapted to specific environment conducted at Kebun Percobaan Fakultas Pertanian Universitas Padjadjaran. Period of research in January 2011 - June 2011, July 2011 - December 2011, April 2012 - September 2012, July 2012 - December 2012, and September 2012 – February 2013 A randomized complete block design with two replicates over five times planting were employed with 33 local varieties as treatments. Combined of Anova integrated with AMMI was applied to detect the genotype x environment interaction, to estimated genetic parameter, and determine adaptation and yield stability.
  • 5. Source df Yield (t/ha) MS (%) Total (%) GE Season 4 1815.2** 32.0 Block(Season) 5 2.3 Varieties 32 268.91** 38.0 Varieties x Season 128 46.57** 26.3 Component AMMI 1 35 85.81** 50.4 Component AMMI 2 33 59.4** 32.9 Component AMMI 3 31 25.1** 13.0 Error 29 7.59 3.7 Residual 160 5.22 3.7 Total 329 68.92 KK (%) 26.61
  • 6. Parameters Trait Yield (t/ha) Var g 22.23 Var f 27.45 Var gm 20.68 Var e 5.22 KKG 54.9% KKF 61.0% KKGM 53.0% SE var g 20.71 Criteria broad SE var f 9.53 Criteria broad H 0.81 Criteria high
  • 7. Local Varieties Season 1 Season 2 Season 3 Season 4 Season 5 Average Bagolo 2,8 d 2,3 b 9,2 d 10,2 e 7,6 d 6,4 Eno 8,5 c 5,9 a 4,8 d 24,1 c 15,6 c 11,8 Ganola 8,5 c 6,7 a 18,0 b 20,5 d 7,4 d 12,2 Jawer Kotok1 16,0 a 5,5 a 21,5 b 24,4 c 18,4 b 17,2 Jawer Kotok2 16,6 a 8,5 a 20,3 b 23,5 c 29,4 a 19,7 Menes Gedang 9,0 c 1,8 b 5,5 d 20,9 d 4,8 d 8,4 Menes01 9,0 c 2,4 b 7,0 d 17,2 d 8,6 d 8,8 Nirkum02 8,0 c 4,3 a 1,5 e 2,1 f 0,8 e 3,3 Nirkum03 7,0 c 4,5 a 5,3 d 13,1 e 3,6 e 6,7 Nirkum06 0,6 d 0,9 b 2,0 e 17,2 d 1,2 e 4,4 Nirkum08 4,6 d 3,0 b 2,2 e 7,2 f 8,8 d 5,2 Nirkum09 6,6 c 4,4 a 3,7 e 16,3 d 1,6 e 6,5 Nirkum12 10,5 b 2,2 b 6,8 d 18,6 d 2,4 e 8,1 Nirkum13 6,5 c 3,0 b 6,0 d 17,4 d 5,0 d 7,6 Nirkum14 1,7 d 1,7 b 0,2 e 14,8 e 1,1 e 3,9 Nirkum16 1,5 d 0,4 b 3,3 e 2,8 f 1,0 e 1,8 Nirkum18 10,0 b 1,5 b 3,0 e 22,1 c 2,8 e 7,9 Nirkum19 7,8 c 3,6 b 0,5 e 17,7 d 0,8 e 6,1 Nirkum20 6,8 c 3,2 b 7,7 d 11,6 e 5,4 d 6,9 Nirkum21 5,3 c 2,1 b 1,7 e 23,4 c 9,8 d 8,5 Nirkum22 10,0 b 4,9 a 0,6 e 35,2 b 4,6 d 11,1 Nirkum23 5,5 c 1,5 b 0,3 e 1,3 f 1,0 e 1,9 Nirkum24 4,5 d 2,7 b 2,0 e 18,2 d 1,8 e 5,8 Nirkum25 6,4 c 3,7 b 1,7 e 11,1 e 1,0 e 4,8 Nirkum26 8,5 c 2,1 b 8,0 d 13,2 e 1,4 e 6,6 Nirkum27 5,2 c 1,7 b 8,2 d 14,8 e 1,4 e 6,3 Menes NK 13,3 a 4,5 a 1,2 e 23,6 c 1,4 e 8,8 Nirkum29 5,6 c 0,2 b 9,0 d 4,1 f 2,2 e 4,2 Nirkum31 6,8 c 1,9 b 2,3 e 9,5 e 3,0 e 4,7 Odos 8,0 c 2,6 b 3,0 e 26,9 c 14,7 c 11,0 Rancing 13,5 a 6,4 a 28,0 a 48,2 a 26,0 a 24,4 Rancung01 11,5 b 9,3 a 13,0 c 20,9 d 20,6 b 15,1 Rancung02 11,2 b 6,7 a 19,3 b 24,7 c 25,0 a 17,4 Average 7,80 3,51 6,87 17,47 7,28 8,6
  • 8. Tuber yield stable over planting 2011 – 2013 : Variety Menes 01, Nirkum 13, and Nirkum 27 Jawer K-2 Menes01 Menes Ged Nir-02 Nir-03 Nir-06 Rancung01 Rancing Bagolo Eno Ganola Nir-08 Nir-09 Nir-12 Nir-13 Nir-14 Jawer K-1 Nir-16 Nir-18 Nir-19 Nir-20Nir-21 Nir-22 Nir-23 Nir-24 Nir-25 Nir-26 Nir-27 Menes-NK Nir-29 Nir-31 Odos M1 M2 M3 M5 M4 -3.0 -2.0 -1.0 0.0 1.0 2.0 3.0 4.0 -6.0 -5.0 -4.0 -3.0 -2.0 -1.0 0.0 1.0 2.0 IPCA2-32.9% IPCA1 - 50.4 % AMMI Biplot GEI of Yield (t/ha)
  • 9.  Yield of local varieties of sweet potatoes is influenced by genotype x environment interactions  There are three local varieties that have a phenotypicaly stable in tuber yield, i.e. variety Menes 01, Nirkum13, and Nirkum27
  • 10.  THANK YOU Sweetpotato ice cream Sweetpotato mini stick Roasted Sweetpotato