GLOSOLAN | Global Soil Laboratory Network: 3rd meeting. 28-30 October 2019 | FAO headquarters, Rome, Italy. Presenter: Ms. Bertsch

1. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
Floria Bertsch
Chair LATSOLAN
Latin American Soil Laboratory Network
(LATSOLAN)

2. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
Latin American Soil Laboratories Network
was launched in Mexico
March 1-3, 2018

3. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
México
Guatemala
El Salvador
Honduras *
Nicaragua
Costa Rica
Panamá
RepDominicana
Jamaica
Cuba
Colombia
Venezuela
Ecuador
Perú
Bolivia
Brasil
Paraguay
Uruguay
Chile
Argentina
Guatemala
Panamá
Jamaica
Rep.Dominicana
Uruguay
Paraguay
Bolivia
Ecuador
Costa Rica
*still outside
ASLAC

4. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
• National Reference Lab are identified in almost every country
• Some SOPs were designed
• A Board Group was established
• A very effective Whatsapp group among
members was established
During 2018
MAIN ACHIEVEMENT
• First “Ring Test” 2018 was executed
(samples distributed during Workshop, results collected in August

5. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
During 2019 • In March, México, the 2nd Workshop of
the Network was realized
• Results of 1st RT and improvements, were
discussed
• Nok was with us
• Some SOPs were armonized with SEALNET
MAIN ACHIEVEMENTS
•Samples for the 2nd “Ring Test” 2019
were distributed
•A Simposia for CLACS in Uruguay was
organized

6. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
RED LATINOAMERICANA
DE LABORATORIOSDE SUELOS
Simposio
“Armonización deAnálisis de Suelos:
su importancia y avances
en Latinoamérica”
Expositors:
1. Importance of 5th Pilar of GSP-Rosa Cuevas, FAO
2. Objectives and advances of LATSOLAN-Jorge Sánchez, LATSOLAN Board, Colombia
3. SOPs developed and used by LATSOLAN-Miriam Ostinelli, Argentina
4. LATSOLAN Ring Test 2018, Report-Floria Bertsch, Chair, Costa Rica
5. Preparation of samples for Ring Tests-Armando Guerrero, ISP Coordinador, México
6. Succesful experiences of Latin American national networks-Ana Silbermann, Uruguay
7. Plans and LATSOLAN perspectives-Daniel Carreira, Vice Chair, Argentina
7-11 Oct, 2019

7. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
OBJETIVOS Y AVANCES EN LA ARMONIZACIÓN DE
LABORATORIOS DE SUELOS A NIVEL DE AMÉRICA
LATINA – LATSOLAN
Floria Bertsch (Universidad de Costa Rica); Daniel Carreira (Laboratorio de Suelos CIRN-CNIA-
INTA, Argentina); Daniel Vidal (Laboratorio de Suelos de EMBRAPA, Brasil); Armando Guerrero
(Coordinador del ISP, México); Jorge Sánchez-Espinosa (Laboratorio Nacional de Suelos, Instituto
Geográfico Agustín Codazzi, Colombia), Luis Cacuango (Agrocalidad, Ecuador), Cristina Suárez
(Laboratorio de Suelos, República Dominicana).
Grupo Directivo LATSOLAN.

8. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
1. Develop mechanisms to harmonize soil
information among labs in the región.
2. Build consensual SOPs.
3. Improve assurance and quality control
(QA/QC).
4. Implement a regional “Ring Test”
5. Promote the exchange of information and
experiences between labs and develop
capacities
OBJECTIVES

9. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
Network Goals
Improve
capacities
Ring
Tests
Protocols
Harmonization
(SOPs)
QUALITY

10. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
 Advantages and Limitants
 Priorities in the region or for GSP
 Adoption potential
 Implementation process
Questionnaires to collect information
Critical point analysis
SOPs writing
Evaluation through RT
Review and improvement of SOPs
Integration with SEALNET, GLOSOLAN, AFRILAB
Discussion
themes}
}Actions

11. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
RT 2018 RT 2019
pH water (1:2.5) LATSOLAN GLOSOLAN
COx Oxydized Organic Carbon (Walkley&Black) LATSOLAN GLOSOLAN/SEALNET
Ct Total Carbon (Dumas) LATSOLAN LATSOLAN
CaCO3 Carbonates own ----
COt CO total (Total Organic Carbon) calculated ----
Nt Total Nitrogen (Kjeldahl) LATSOLAN LATSOLAN
Nt Total Nitrogen (Dumas) LATSOLAN LATSOLAN
K Exchangeable K (Ammonium Acetate) LATSOLAN LATSOLAN
Ca Exchangeable Ca(Ammonium Acetate) LATSOLAN LATSOLAN
Mg Exchangeable Mg (Ammonium Acetate) LATSOLAN LATSOLAN
CE SEALNET
Na LATSOLAN
P own
Parameter
SOPs applied by LATSOLAN in both RT

12. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
SOPs, TABLE OF CONTENTS
1. INTRODUCTION
2. SCOPE AND FIELD OF APPLICATION
3. PRINCIPLE
4. APPARATUS
5. MATERIALS
6. HEALTH AND SAFETY
7. SAMPLE PREPARATION
8. PROCEDURE
9. CALCULATION
10.QUALITY ASSURANCE / QUALITY CONTROL
11.REFERENCE DOCUMENTS
12.APPENDIXES

13. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
RT
LATSOLAN (2018)
ISP-51
Own
protocols
Harmonized
protocols
ISP-52
ISP-53
3 replications

14. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
México
We must and want
recognize
the valuable contribution
of
In the first steps
of LATSOLAN
With the financial support of the AMS / FAO
• Hosted the first 2 workshops
• Prepare the test items of the first two RT

15. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
IQR = Q3 - Q1.
Q1 = first quartil
Q3 = third quartil
OUTLIER if
xi <Q1 - (1.5 x IQR)
xi> Q3 + (1.5 x IQR)
1,5
Ẋ = Consensus Value
MEAN
Standard Deviation (S)
Statistics process, harmonized with SEALNET
“z value”
Outliers

16. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
pH
ISP-51
ISP-52
ISP-53
z of
replications

17. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
K in Ammonium Acetate

18. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
32%
26%
28%
27%
25%
25%
27%
27%
29%
39%
48%
0%
10%
20%
30%
40%
50%
60%
pH Ct COX NtKj Nt K Ca Mg
%CV
ISP-53 ISP-52 ISP-53
%CV Without IQR adjustement

19. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
-2,00
-1,00
0,00
1,00
2,00
3,00
4,00
5,00
6,00
7,00
8,00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
cmol(+)Mg/kg Acetato de Amonio ISP-53
147%
98%
98%
147%
ISP-53 Mg
%CV =49% with IQR de 1.5
150% !!!!
150%!!!!
0.33 cmol(+)/kg Mg
4,57 cmol(+)/kg Mg

20. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
32%
26%
28%
27%
25%
25%
27%
27%
29%
39%
48%
0%
10%
20%
30%
40%
50%
60%
pH Ct COX NtKj Nt K Ca Mg
%CV
ISP-53 ISP-52 ISP-53
24%
21%
21%
23%
22%
0%
10%
20%
30%
40%
50%
60%
pH Ct COX NtKj Nt K Ca Mg
%CV
ISP-51 ISP-52 ISP-53
Without IQR adjustement With IQR adjustement

21. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
0,00
1,00
2,00
3,00
4,00
5,00
6,00
7,00
8,00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
cmol(+)Mg/kg Acetato de Amonio ISP-53
54%
36%
36%
54%
ISP-53 Mg
36%
36%
1,73-3,07 cmol(+)/kg
0,33-4,57 cmol(+)/kg
%CV =18% with IQR of 0,75
-2,00
-1,00
0,00
1,00
2,00
3,00
4,00
5,00
6,00
7,00
8,00
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
cmol(+)Mg/kg Acetato de Amonio ISP-53
147%
98%
98%
147%
%CV =49% con IQR de 1.5
150% !!!!
150%!!!!

22. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
ISP-51 0,85 - 2,27 0,85 - 1,68
ISP-52 0,64 - 2,07 0,64 - 1,17
ISP-53 1,11 - 3,06 1,11 - 2,10
COx Analysis
With IQR=1,5 With IQR=0,75
%CV=36% %CV=24%
%CV=40% %CV=20%
%CV=36% %CV=23%

23. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
LAB pH Ct COX
Nt
Kje
Nt K Ca Mg
#Items
Insatisfactorio
%INSAT
#Ensayos
Insatisfactorios
%Insatisfactorios
ISP-51 ISP-52 ISP-53 ISP-51 ISP-52 ISP-53 ISP-51 ISP-52 ISP-53 ISP-51 ISP-52 ISP-53 ISP-51 ISP-52 ISP-53 ISP-51 ISP-52 ISP-53 ISP-51 ISP-52 ISP-53 ISP-51 ISP-52 ISP-53
2 S S S S S S S S S S S S S S S I S S S S S S S S 24 1 4% 8 1 13%
3 S S S S S S S S S S S S S I S S S S S S S 21 1 5% 7 1 14%
4 S S S S S S S S S S S S S S S 15 0 0% 5
5 S S S C S S S S S S S S I I I S S S S S S 21 3 14% 7 1 14%
6 S S S S S S S I C S S S C S C S C I 18 2 11% 6 2 33%
7 S S S S S S S S S S S S S S S S S S 18 0 0% 6
10 S S S I I S S S S S S C S S S S S S 18 2 11% 6 1 17%
11 S S S S S S S S S S S S S S S S S S S S S S S S 24 0 0% 8
12 S S S S S S I I S I I C S S S C I I 18 6 33% 6 3 50%
13 S S S S S S S S S S S S C C C S C I 18 1 6% 6 1 17%
14 S S S S S S S S S S S S S S S S S S S S S 21 0 0% 7
15 S S C S S S S S S S I I C C C S C I 18 3 17% 6 2 33%
17 S I I S S S I I I S S S S I I 15 7 47% 5 3 60%
18 S S S S S S S S S S S S S S S S S I S S S S S S 24 1 4% 8 1 13%
19 S S S S S S S S S S S S S S S S S S S S S 21 0 0% 7
20 S S S S S S I I I S S S S S S S S S C I I 21 5 24% 7 2 29%
315 32 10%
# Labs 16 9 16 11 6 16 16 15
# No total satisf 1 0 3 2 0 6 1 5
% No total satisf 6% 0% 19% 18% 0% 38% 6% 33%
Counting all the 315 units analyzed, 90% were satisfactory

24. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
By essays, only Excheangeable K y Mg in Ammonium Acetate
presented problems inn more than 5 laboratories (33%).
Others (80% of labs) were satisfactory.

25. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
# C+I % # I %
4 5
7 6
11 8
14 7
19 7
2 8 1 13% 1 13%
18 8 1 13% 1 13%
3 7 1 14% 1 14%
5 7 2 29% 1 14%
10 6 1 17% 1 17%
13 6 1 17% 1 17%
20 7 1 14% 2 29%
6 6 1 17% 2 33%
15 6 2 33% 2 33%
12 6 3 50% 3 50%
17 5 3 60% 3 60%
72 12 17% 13 18%
IdLAB
#Ensayos
Lab Cuest+Insat
en procedimiento
convencional1
Lab Insat
con ajuste a
20%CV2
By laboratory,
from 16,
5 presented 100% of satisfy,
6 had a very good behaivor,
and only 5 had less than 80%

26. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
#LAB
ISP 51 52 53 51 52 53 51 52 53 51 52 53 51 52 53 51 52 53 51 52 53 51 52 53
2 0,0% 0,0% 0,0% 1,2% 1,1% 0,9% 0,6% 0,9% 2,1% 8,4% 5,6% 0,0% 3,8% 5,6% 3,0% 6,7% 1,6% 1,8% 1,7% 1,7% 0,9% 2,6% 2,6% 2,8% 4
3 0,8% 0,7% 0,0% 1,7% 2,3% 0,5% 0,4% 0,9% 0,8% 0,0% 0,0% 0,0% 2,6% 0,8% 2,2% 0,9% 3,6% 1,7% 4,9% 1,9% 1,5%
4 0,0% 2,0% 1,3% 2,6% 5,7% 4,7% 6,3% 1,6% 3,9% 4,8% 1,1% 2,8% 4,9% 4,3% 3,8% 2
5 0,8% 0,0% 0,0% 2,1% 3,3% 5,7% 4,3% 1,3% 2,7% 3,7% 8,3% 0,0% 7,6% 1,6% 3,4% 6,0% 3,3% 1,4% 1,9% 4,6% 6,8% 5
6 2,3% 1,6% 0,0% 2,8% 4,3% 4,6% 0,0% 18,4% 13,3% 4,3% 1,5% 16,1% 16,3% 6,0% 4,6% 13,6% 9,9% 8,5% 8
7 0,9% 0,8% 0,0% 5,6% 6,7% 3,7% 0,0% 0,0% 0,0% 2,1% 3,0% 2,0% 2,3% 1,5% 1,5% 0,8% 3,8% 2,5% 2
10 0,8% 0,0% 0,0% 1,8% 2,0% 1,7% 9,4% 6,0% 2,8% 2,3% 5,3% 2,2% 2,6% 6,2% 1,8% 4,9% 4,5% 1,8% 4
11 0,8% 0,7% 0,7% 0,4% 1,0% 0,5% 2,9% 3,4% 4,6% 0,0% 0,0% 3,8% 0,0% 5,6% 0,0% 4,4% 10,6% 1,5% 3,8% 1,7% 3,9% 3,2% 1,1% 2,0% 2
12 0,8% 0,0% 0,7% 0,8% 0,0% 0,3% 5,6% 9,1% 12,6% 5,2% 2,7% 3,1% 0,1% 0,1% 0,0% 0,3% 0,6% 0,0% 4
13 0,9% 0,0% 0,0% 1,0% 0,8% 0,8% 4,9% 0,0% 0,0% 0,0% 0,0% 0,0% 0,8% 0,5% 2,0% 0,0% 1,8% 1,3%
14 0,0% 0,0% 0,0% 0,9% 0,9% 0,5% 2,2% 2,2% 0,8% 4,6% 5,1% 3,3% 15,3% 4,4% 4,0% 13,7% 5,2% 2,5% 16,5% 3,7% 4,0% 5
15 0,8% 0,0% 0,7% 1,9% 0,0% 0,0% 0,0% 5,6% 0,0% 5,3% 3,2% 13,6% 16,6% 13,1% 14,9% 14,8% 11,6% 16,8% 9
17 0,9% 0,0% 0,0% 5,6% 0,0% 2,0% 12,7% 2,3% 2,1% 0,0% 0,0% 0,0% 1,1% 1,8% 0,5% 2
18 0,0% 0,0% 0,0% 0,9% 1,6% 0,8% 0,8% 0,6% 1,1% 0,0% 0,0% 0,0% 0,0% 0,0% 3,0% 2,6% 1,3% 0,7% 0,4% 0,1% 0,3% 0,4% 0,3% 0,4%
19 1,4% 0,0% 0,7% 1,3% 1,1% 1,1% 1,7% 2,4% 3,4% 6,7% 0,0% 2,8% 0,0% 0,0% 0,0% 1,2% 1,2% 1,5% 0,8% 0,4% 2,1% 1
20 0,0% 0,7% 0,7% 0,9% 1,2% 1,6% 2,0% 2,8% 2,8% 4,2% 0,0% 2,7% 7,6% 5,6% 8,2% 4,3% 1,4% 1,8% 1,0% 0,9% 4,4% 3
51
Ca Mg
#udconmás
5%DEint
pH Ct COx Nt Kj Nt (Dumas) K
0 2 4 11 4 13 9 8
0 y 18.5%
51 more than 5%
18 more than 10%

27. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
RECOMMENDATIONS:
• Exclude CaCO3 and COT
• Apply more demanding statistical tests (% CV <15-
20%)
• Improve behavior in next RT
• Use samples with different values
• Use the "median" instead of the "mean" as a
consensus value
• Each lab must identify their problems
• Compare the impaired results with the results of
the protocols themselves.

28. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
• Argentina (2006): (RILSAV)
• Brasil (1992): (PAQLF)
• Costa Rica (1998): (PINAS)
• Ecuador (2001): (RELASE)
• México (1997): (REDLABS)
• Colombia (1995): SCCS
• Uruguay (2014)
• Nicaragua (2019)
Some National Network
All Networks
have a
complete
scope in the
country

29. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
RN
GLOSO
LAN
National Network
Reference Lab
Directive Board
RN
RN
RN RN
RN
RN
RN
RN
RN
RN
RN
RN
Structure and
relations

30. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
RESOLAN: Redes Regionales
2018
2019
2
0
1
9
2017
2019

31. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
Last agree of LATSOLAN:
We are working in it!

32. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
IN SUMMARY…
• Achieve the conformation of all National Networks…
• Advance the harmonization of SOPSs
• Continue with the RT
• Have another Workshop next year in Brasil
• Coordinate support, training and laboratory assistance actions

33. 3rd Meeting of the Global Soil Laboratory Network (GLOSOLAN)
Thanks for your attention