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Hw1 Gen320fall07revised

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Genetics 320 REVISED HW 1. Due Sept5, Wed. 11AM. TEXT EXPLANATIONS MUST BE TYPED 1. Complete the following chemical structure. I AM ASKING FOR A C-C DINUCLEOTIDE HYDROGEN-BONDED TO A G-G DINUCLEOTIDE. THE DOTTED LINES INDICATE THE AXIS OF EACH DINUCLEOTIDE. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],5’ 5’ O-P=O O O-P=O N O N N N O N O N N C-G C-G H H H H H
[object Object],[object Object],[object Object],[object Object]
3. Consider the domain structure and the phenotypes of mutants in the gene shown below. The three domains are shown (amino acids 10-100 for the rectangle, 120-150 for the triangle, and 180-210 for the circle). The gene has two functions, one in memory and one in hearing. The gene has no introns. Cells (wt or “wildtype”=normal; mut1, mut2) are homozygous for each allele. Amino 1 10 100 120 150 180 210 250 Acid # Cell Wt mut1 mut2 Cell Phenotypes Hearing memory ,[object Object],[object Object],[object Object],[object Object]
4. Describe the fate of “the p1 protein” for each of the mutants listed. In the p1 protein the specific amino acids 1,2,3 and 6 are essential for function; amino acids 4 and 5 are “linker” and can be any amino acid. p1 synthesis requires DNA damage and the p53 enhancer (see below). Describe a likely consequence of each mutation; is a transcript made , is the transcript translated, how long is the translated protein, and the overall amount of functional protein in the cell. Bases in the TATA box that are essential for RNA polymerase binding are capitalized. Capital letters in the ORF are to help you identify the amino acids essential for function. Consult Genetic Code Table. You can answer with More, Less, Unchanged compared to wt. p53 binding site TATA box +1 12 TATAccg ggccaattgcg A TGGGCATA cccctg CCCTGA ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Transcript Translated #AA Function p53 biology: p53 is a transcriptional activator, or enhancer. Without it, the RNA polymerase cannot transcribe the p1 gene very well.
5. Describe briefly how a gene family arises. The gene family can consist of two genes with related but slightly different functions. 6. Discuss briefly the relevance of the idea that ATP is not limiting for many processes in higher eucaryotic cells for the presence of introns.

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Hw1 Gen320fall07revised

  • 1.
  • 2.
  • 3.
  • 4.
  • 5. 5. Describe briefly how a gene family arises. The gene family can consist of two genes with related but slightly different functions. 6. Discuss briefly the relevance of the idea that ATP is not limiting for many processes in higher eucaryotic cells for the presence of introns.