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Agri tech Investors Meet

Agri tech Investors Meet

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  • 1. Agri- Tech Investors Meet
  • 2. Agri- Tech Investors Meet 2 Looking to start your own Agribusiness Venture? Want to become the Change-maker in your Community? Get a chance to know more about the potential of business incubators and how they can support your venture at our agribusiness pavilion.To know more about the technologies on display, visit the technologies page or click here. And if you are interested in any of the technologies ? Then meet our Business Managers for a quick chat at the deal-making room to start your dream agribusiness venture. It‟s that simple! The event is open to: entrepreneurs, innovators, funding agencies, development organizations and anybody connected with the agricultural sector. The event will feature: B2B/B2C meetings for technology commercialization and partnership engagement Two-day agri-exhibition featuring more than 80 ready-to-commercialize agri- technologies from crops, horticulture, food processing, animal husbandry, fibres, agri-engineering, industrial processing and fisheries sector Agribusiness incubators and their services. List of Technologies Food Processing o Tender coconut beverage with suspended kernels Tender coconut water is the best healthy soft drink that nature provides. This natural product is collected and preserved as beverage. The low-cost technology is refined and ready for commercialization, and can be considered by agri-SMEs and farmer groups. The market potential of the product is large and is currently dominated by carbonated, soft-drinks, which are not healthy. The product is an ideal, healthy replacement o Virgin coconut oil meal (VCM) based products viz : biscuit, compressed bar, burfi, ladoo, porridge and noodles VCM is a bi-product of virgin coconut oil and rich in fiber and proteins. The meal can be a base to develop biscuit, compressed bar, burfi, ladoo, porridge and noodles. Desiccated coconut is mixed with VCM for the production of coconut burfi. Dates syrup, sugar fried cashew nuts, milk and wheat flour are the various components used in the production of various VCO meal products. o Sweet coconut chips
  • 3. Agri- Tech Investors Meet 3 An innovative product, coconut chips is a low caloric value, rich in fiber and ready-to-eat snack. It is not deep fried unlike others that use edible and non- edible oils such as palm-oil which have lower nutritious value. Coconut chips contain no additives and artificial sweeteners. o Virgin coconut oil Traditionally coconut oil is prepared from sun-dried copra. The new technology was developed to produce oil from coconut milk that gives virgin coconut oil. The technology has been refined and two methods were developed for production of the oil, which contains more phenolic content, antioxidant activity than other commercial grade oils which are having lower nutritional value. o Bajra lassi; Milk protein enriched iron-fortified biscuits from composite dairy millet mix; and pearl millet & milk solid based Nutrimix The three products i.e. Bajra lassi, milk protein-enriched iron fortified buiscuits from a composite dairy-millet mix and nutrimix based on pearl millet & milk solids belongs to composite dairy foods category. All these products have been developed to harness the nutritional virtues of under-utilized pearl millets and milk solids specially the dairy by-products i.e. whey. Whey is a major dairy by- product and it exhibit high biological value due to its unique amino acid composition. Pearl millet is major millet crop grown in rain fed agro-climatic zones is quite rich in micronutrients, possess unique amino-acid profile and dietary fiber, but presence of anti-nutrients, polyphenolics and pigmentation limits their application for food purposes. Increasing micronutrient deficiency and protein malnutrition can be alleviated through the utilization of whey, milk solids and pearl millet in food products. Intervention of the micronutrient and protein deficiencies through concept of composite dairy cereal mix led to the development of bajra lassi and high protein biscuits from composite dairy cereal mix. o Insta Idly Dry Mix Idly is a popular breakfast dish in peninsular India. Traditional idly preparation takes at least 14-18 hrs of preparatory work, including wet grinding that is a cumbersome process. All this is eliminated in this innovative technology that uses dry grinding process and reduces preparation time to 2 hrs. The fermented batter is steam cooked by usual method. o Millet Dhokla The innovative, millet dhokla mix is allowed to ferment for 1 hour with addition of lime juice and curd at room temperature. The batter is then poured into a greased pan and steamed, cooled to the room temperature and cut into pieces, seasoned with mustard seeds, green chillies and coriander leaves. This technology improves the nutrient value and reduces fermentation time from 9 hours to just 1 hour.
  • 4. Agri- Tech Investors Meet 4 o Development of process technology for ginger extract standardized to >20% w/w total gingerols as a water soluble powder As the name oleo-resin indicates, ginger oleoresin predominantly contains ginger oils and resins which are both water insoluble. A special process has been developed whereby standardized ginger extract having > 20% w/w total gingerols in water soluble form can be produced. The powder has application in food and pharma sector. o Development of process technology for ginger extract standardized to > 30% w/w total gingerols as a free flowing powder Another innovative process for producing standardized ginger extract having > 30% w/w total gingerols in a free flowing powder form, which prevents handling losses which are generally very high when the ginger oleoresin is in the form of a sticky thick paste. Ginger extracts having high gingerol content are preferred in the market as small quantities are sufficient to deliver the required therapeutic effect in the nutraceuticals / dietary supplement industry. o Technology for successive water extract of ginger (NR-Ginger) for cholesterol management After the extraction of oleoresin from ginger, the spent material is generally thrown away. An innovative process has now been developed that can be used for preparation of successive water extract from ginger spent. This extract was further fractionated to achieve desired bioactivity and water solubility. In lab animal models, the extract showed comparable cholesterol lowering activity to statins. This is being positioned as a unique extract of ginger in the international market and can help in cholesterol management. o Juli coffee (Instant coffee) Prosopis juliflora is found in abundance in arid and semi-arid tropic of India. Rajasthan, Gujarat, Tamil Nadu and Haryana are states where pods of the species are available in very high quantity. The species produce pods from December to May which are highly liked by livestock. The ripened pods of the plant are thrashed in a specially designed plot thresher and layers is separated and sieved. By threshing and sieving 20 kg pods, approximately 5kg fine mesocarp is obtained which is used for processing Juli coffee. This fine mesocarp powder is roasted at 250o C for half an hour in a pre-heated oven with intermittent mixing and blended with 20% original roasted coffee powder. After mixing original roasted coffee powder, 10 % chicory powder is mixed in this mixture which gives taste, colour and aroma of normal instant coffee. For obtaining best and highly soluble Juli coffee the mixture obtained above is dissolved in water @ of 100g mixture in 200 ml water and filtered. In this way 90 g Juli coffee powder is obtained which is freeze dried for 9-10 hours. After completing this process 70g best quality Juli coffee can be obtained. Analysis of
  • 5. Agri- Tech Investors Meet 5 current market trends of instant coffee brands indicate average cost of Rs. 108 per 100 g. Against this, the estimated cost of Juli coffee is Rs. 45/ 100g. o Prosopis juliflora pods based cheaper concentrate mixture as economical cattle feed The pods of Prosopis juliflora is liked by livestock and can be used to reduce cost of concentrate ration, while at the same time offers higher nutritive value when compared to currently available feed in market. o Banana pseudostem central core candy Central core of banana pseudostem is rich in digestible fibres, Iron, Vitamins B3 and B5. Hence candy made from central core of banana pseudostem is rich in these nutrients. After processing of central core into candy it is delicious and palatable. o Capsicum salsa Capsicum salsa is an innovative ketchup/sauce like product from whole capsicum or with blend of tomato, spices and condiments. The technology is cost effective and process involves hot breaking, maceration, concentration and stabilization with permitted preservatives. The product has natural red colour from capsanthin from red capsicum, has a characteristics flavour and thus high visual appeal. It provides a suitable alternative to existing „tomato sauce”. The product is high in functional quality and an excellent source of ascorbic acid, antioxidant phenolics and flavonoids. Agri-Engineering & Textiles & Industrial Processing o Pollution Preventing System (PPS) for controlling the effluents emitted from the traditional drum roasting process in cashew factories The system is designed to remove heavy carbon particles as well as the polluting gases generated during the conventional drum roasting process in cashew factories leaving only steam outside. o A new non thermal technology for cutting and peeling of raw cashew-nut Incubating with certain type of hydroxyl ion producing mixture was found feasible for cutting the cashew shell as well as peeling the testa. This technology was standardized for cutting open the raw cashew nut without thermal application. o Low cost method for processing technology for extraction of anacardic acid New extraction process for anacardic acid from cashew shell was developed without thermal application in salt form and can be stored as anacardate. The compound anacardic acid has the property to be anti-cancerous, antibacterial etc.
  • 6. Agri- Tech Investors Meet 6 By using cashew shell as a raw material, the process will help in utilization of cashew industry by product (cashew shell) and thereby making it into a very good foreign exchange earner compound. o Browning free custard Apple pulp extracting technology An innovative technology has now been developed for mechanized extraction of pulp from custard apple fruit. The machine is in two parts: first part scoops out the pulp with seed from fruit leaving behind the peel. The second part separates the seed from pulp which can be stored for up-to one year. Potential users can be Ice-cream Industry, Dairy and Food processing Industry, Bakery and Confectionary Industry etc. Cold storage temperature required for this pulp is -20 degree C with -30 degree C in the beginning (Blast freezing). o Value Chain on Castor and its Industrial Products Developed products based on 2-octanol such as Dicapryl phthalate, Dicapryl sebacate, Dicapryl maleate, Dicapryl fumarate, Dicapryl adipate, Dicapryl ether, Capryl glycidyl ether, 2-octene, etc. Developed process technology for extraction and isolation of the protein from deoiled castor cake. Developed adhesive formulation for industrial applications (wood) using castor protein. o Cryo-grinding unit for value addition in seed spices Cryogenic grinding is a method of powdering herbs and spices at subzero temperatures ranging from 0 to -195 degree Celsius. The herbs and spices are frozen with liquid nitrogen as they are being ground. This process does not damage or alter the chemical composition of ground material in any way. The low temperature can save volatile components and heat sensitive constituents in herbs and spices. o Non-woven fabric preparation from banana pseudostem and extraction of fiber using raspador Extraction of fiber using raspador and making non-woven fabric is a unique innovation which converts banana pesudostem waste into wealth and hence a boon for banana farmers. The technology has a potential to revolutionize the banana economy of the country. o Flexible Rubber Dams (Rubber-Textile composite for watershed management) A rubber dam or flexible check dam is an inflatable structure build across a stream used for water conservation, flood control and regulating flow of water in the stream. When it is inflated, it serves as a check dam/weir. And when it is
  • 7. Agri- Tech Investors Meet 7 deflated, it functions as a flood mitigation device and sediment flushing. The head or height of rubber dam is variable and helps regulate the depth of flow in the diversion channel for irrigation present in the upstream side of the check dam. o Nano cellulose Production Technology Production of nanocellulose is an energy intensive process which affects its scaling up for commercial production. In spite of being many processes available for energy reduction, they are limited to pilot scale and not yet reached the commercial production. The technological innovation deals with the reduction in energy consumption during production of nanocellulose from cellulosic biomass in an eco-friendly way. o Technology of dyeing cotton and silk with natural dyes Until the turn of the century, fabrics were coloured using natural dye stuffs obtained from nature as they were the only colouring substances available at that time. Natural dyes are extracted from the roots, stems, leaves, flowers fruits and seeds of various plants and dried bodies of certain insects and minerals. The innovative technology has developed dye shades from 8 sources to dye cotton (7949 shades), silk (2431 shades) and banana fibres (150 shades). The gray scale readings of colour fastness ranged from 3.5 to 5, which mean good to excellent fastness to sunlight, crocking, perspiration and washing. These shades are recommendable for application. o Eco-Powders The innovative technology provides more than 6 eco- powders with improved shades and replicability besides environmental safeguards. o Eco-Paints Presently 15 shades of paints are produced with this technology, which can be spray dried suitable for larger scale production, as well as used on varied decorative products. o PCR based quality estimation technology for purity of Pashmina wool Pashmina, popularly known as 'Cashmere', is well known for its fineness, warmth, softness, desirable aesthetic value, elegance and timelessness in fashion. In India, bulk of Pashmina fibre is used for the manufacture of shawls, mainly in Kashmir valley. The fabric produced from Pashmina is usually handspun and hand woven product. The processing of Pashmina involves lot of labour in sorting, spinning, weaving and dyeing which are usually done manually. Being very expensive, it is generally adulterated with other animal fibre and thus this technology for identification of animal fibers is a very innovative step towards limiting adulteration in Pashiman wool.
  • 8. Agri- Tech Investors Meet 8 The technology is PCR based protocol that has been developed to identify origin of animal fibre especially Pashmina in woollen products. Quality DNA has been extracted from animal fibres and their blended products. The technique is able to amplify Pashmina genes and sheep wool to identify the blend up to 10% level. It is a quality control tool to measure the purity and authentication of prepared garments made from pure Pashmina. It could be used for quality control inspectors of the State Departments and quality control wing of the industries. o Improvisation of traditional charkha The pashmina fibre undergoes a series of processing steps viz; dehairing, spinning, weaving, and finishing etc in order to get a finished product. Spinning is a processing step, usually accomplished by the women folk of both rural and urban areas on a traditional spinning wheel locally known as Yender. The spinner operates the charkha in a specific sitting posture. It is an arduous task as it causes lot of physical stress leading to backache, numbness in the legs and several other associated problems. Besides that the job is less remunerative to the spinners. Keeping in view, the popularity of Pashmina shawls, difficult processing steps and less remuneration, an innovative Table Top Paddle Operated NAIP charkha was fabricated, so as to improve the efficiency of spinning and thereby remuneration of spinners besides reducing the physical drudgery and at the same time keeping the originality (Hand spun Hand woven status) of the product intact. Veterinary & Livestock o Animal feed with potato waste The technology utilizes the by-products from the potato processing industry that leaves 40% undersized unusable potato and their peel as by-product by converting it into animal feed that can be used for feeding about 300 million cattle and buffalo population which is a major challenge. Potato processing waste can efficiently be used to replace grains used in costly cattle feed. o A novel micro-technique & media for selective enumeration and detection of Enterococci in milk The technology involves application of specific enzymatic reaction in selective medium. The marker enzyme which participates in unique biochemical pathways of specific genera or strain hydrolyze chromogenic substrate complex and release free chromogen which can be detected visually by color change (Patent Reg. No. IPR# 119/ DEL/ 2012). Currently, commercially available media like citrate azide agar requires an incubation period of 72-96 hrs for detection of Enterococci in milk. The development of this new “Single Stage Micro Technique” is to facilitate its use in R &D institutions and dairy industry for rapid detection of Enterococci. The medium is highly selective for Enterococci and allow its detection in single working day. The technology has the potential to replace the existing medium for Enterococci for being cost effective (Rs 98.3 per liter) as against Citrate azide
  • 9. Agri- Tech Investors Meet 9 agar (CAA) available @ Rs 262.5 per liter and Bile Esculin azide agar available @ Rs 493.5per liter. o Two stage enzyme assay for detection of L. monocytogenes in milk Two-stage bio-assay has been developed for detection of L. monocytogenes based on the principle of targeting enzyme-substrate reaction for specific marker enzyme(s) to release free chromogen that can be visually detected by colour change (Patent application IPR/ FA/ 12037-L/2012). The assay confirms the presence of L. monocytogenes within real time of 4.30±0.10h after initial pre- enrichment of food samples in novel selective medium i.e. LSEM for 18/ or 24h as against 5-7 days protocol following conventional method (ISO: 11290-2:1998). o Spore inhibition based enzyme substrate assay for monitoring Aflatoxin M1 in milk The developed technology works on the principle of spore germination inhibition if there is Aflatoxin M1 in milk. In case Aflatoxin M1 is absent, the marker enzymes are released during germination which changes the colour of chromogenic substrate to sky blue colour. However, in presence of Aflatoxin M1, the spore germination process is inhibited at ≥ MRL (0.5 ppb) level of Aflatoxin M1 and no change in colour of substrate indicates the presence of Aflatoxin M1 when pre-treated milk along with chromogen is incubated at 37±2oC for 45 ± 5 min. o DNAzyme based lead biosensor Two immobilization strategies have been optimized for DNAzyme based studies. In first method, DNAzyme has been immobilized in TMOS based hydrosol gel in a microarray plate and the microarray analysis of lead in hydrosol gel system has been successfully miniaturized up to 10µl volume, with a detection limit of 0.12 nanomoles. In the second method, immobilization of fluorophore bearing DNAzyme through SAM on gold solid phase surface has been achieved. In this case, 6 X 10-3 nanomoles bio-sensing of lead at 5µl volume has been achieved in synthetic and milk samples. o Recombinant whole cell cadmium biosensor This involves development of a recombinant biosensor plasmid pNV12 by integration of cadC promoter upstream to gfpmut3a of expression vector pAD123. A recombinant biosensor is developed by transforming pNV12 in E. coli DH5α. Recombinant biosensor showed specificity towards cadmium, with a detection limit of 10µg/l and a response time of 15 min. No interference is observed by Pb & Zn, and the system requires no sample pre-treatment. o Microarray based heavy metal optical biosensor Realizing need for quick sampling of milk, multiple sample analysis microarray based heavy metal optical biosensor has been developed with the Bioassay
  • 10. Agri- Tech Investors Meet 10 principle based on inhibition of urease activity. Microbial whole cells and acridine orange (used as florescent indicator) are co-immobilized with hydro sol-gel (Ethanol TEOS) approach in microwell plate. Urea is converted to ammonium and bicarbonate with urease activity that result in protonation of acridine orange and consequently decrease in fluorescent count. In presence of Cd in the reaction mixture (sample) urease activity gets inhibited thereby lesser ammonium production and consequently increased fluorescent count. In comparison to control fluorescent count increase with increasing concentration of the analyte i.e. Cd. Detection limit of 0.001 mg/l of Cd equivalent has been achieved well below the permissible limit for water as defined by EPA (i.e. 0.005mg/l). o Novel phytoacaricide for the control of chemical acaricide resistant tick infestations in animals All most all dairy and meat animals suffer from tick infestations throughout tropical countries including India. Continuous and indiscriminate use of synthetic chemicals, which is the commonly adopted measure to control ticks, has developed resistance In them. Besides, the milk and meat product is regularly contaminated due to residual effect of these chemicals. Human health also suffers due to excessive use of these chemicals. To solve this critical problem, two novel phyto-acaricides have been developed which are safe, non-toxic to animals and human, non-contaminating, with less chances of development of resistance and can tackle the specific problem of controlling chemical resistant tick populations. The product has been tested at field condition and found effective. The products have commercial potential both nationally and internationally. Big companies having infrastructure for large scale production of the product will definitely be interested for commercialization of the valued product. o PCR based DNA test for the differentiation of cattle and buffalo meat and milk This innovation is able to differentiate genomic DNA of cattle and buffalo origin utilizing the size differentiation as well as positive negative amplification of PCR amplified products from single set of primers simply by agarose gel electrophoresis. This technology is already optimized and superior in terms of reliability, since it further confirms the origin of samples to be assured using second set of primers capable of giving positive or negative test also. The technology developed is novel, simple, easy to adopt and cost effective as compared to costlier and cumbersome techniques of PCR-RFLP, sequencing, real- time PCR and SnapShot utilized by other workers. It can be utilized for differentiating cattle and buffalo milk and meat samples and will be useful in detection of advertent mixing of both as well as in vetro-legal cases. o Parentage verification kits for (i) goat and (ii) other ruminant livestock species
  • 11. Agri- Tech Investors Meet 11 Parentage analysis of livestock animals is becoming increasingly important in animal husbandry to improve the accuracy of selection and to select sires to increase production and reproduction traits. Increased use of Artificial Insemination and selection of superior males for various economic traits are the basic established tenets of scientific animal breeding. Recent times have seen usage of DNA based markers for parentage control especially in horses, canines and cattle for which the commercial kits are available. No parentage verification kit was available for other livestock species. Five kits for parentage verification has now been developed which cover most of the livestock species. These are parentage verification kits for buffalo, cattle, camel and goat. A single parentage verification kit for all the ruminant livestock species which include cattle, sheep, goat, yak, mithun and buffalo has also been developed. These are the first kits of DNA based markers for parentage/paternity. A core set of 15 microsatellite markers each from cattle genome database have been identified which are amplifiable in a single multiplex PCR reaction in cattle and buffalo and 14 markers in goat. Similarly, markers were identified for camel which have been pooled together to form a single multiplex PCR reaction for simultaneous amplification of all the markers. The markers selected are highly polymorphic, have high heterozygosity, located on different chromosomes and provide a very high probability of exclusion. o Mineral Based-Technology for estrus induction and synchronization in bovines for increasing the milk production This unique mineral based technology is a simple, cheaper and highly effective. This targets the follicular and corpus luteum development besides toning the uterus for conception. The minimum estrous induction rate in random population of cows is above 85% while in seasonal breeder like buffaloes it is above 72% encompassing all the ecosystem/agro-climates of the country. No comparative technology exists anywhere. The nearest technology is area specific mineral mixture or special supplements which are able to induce estrous only in 25-35% animals and comparatively costly. o Production of cooked and smoked pork sausage, Smoked pork,Pork Pickle with bamboo Shoot and Bhoot Jalakia. Rapid change in life-style and food habits, rising prosperity have created an unprecedented demand for value added processed food products. Traditionally, the people of the North Eastern region of India have a strong preference for foods of animal origin and meat is in great demand in the region. However, due to severe shortfall in processed pork products, the region imports huge quantity of processed meat product from outside. Hence, there is a great scope of marketing value added pork products like sausage in the NER. o Low cost multiplication technology of salt tolerant bio-growth enhancers (Bacillus, Pseudomonads & Trichoderma) for increasing productivity of agri-horti crops in normal and sodic soils
  • 12. Agri- Tech Investors Meet 12 Continuous use of chemical fertilizers and systemic pesticides changes the micro- environment around root zone of plants and the changed environment does not support the growth of beneficial soil microbes. Moreover, the use of chemical fertilizers is limited to NPK and indiscriminate use of chemical pesticides to control pest and diseases is widely prevalent. Bacillus is well known soil microbe for nutrient uptake while Pseudomonas and Trichoderma are well known for their plant protection properties apart from growth regulation. Even though considerable amount of isolates are available in the country as biofertilizers and biocontrol agents, the strains are specific to particular region or soil pH. Moreover, the cost of commercial product ranges from Rs. 80-150 /kg in the open market and public sector institutions. Generally farmers use single microbe and thus, the benefit is also limited. Due to higher cost, limited benefit and area specificity, small and marginal farmers have reservation over its use and prefer chemical fertilizer and fungicides. Moreover, there is no formulation that is well validated for the sodic lands to increase their productivity. Thus the present technology was developed to produce a consortia of beneficial microbes which have universal applicability, higher self-life and lowest cost of production. o Low cost technology for prevention and treatment of sub-clinical and clinical mastitis in bovines Mastitis is a serious problem in milch animals and no effective prevention or full proof therapeutic technology is available to control this complex inflammatory disease. It threatens the profitability of dairy ventures and situation is critical when animals reared by poor farmers for their livelihood, are affected by the disease. Once the fibrosis occurs there is no reversal and slaughter is the only option. The consumers consuming such milk are also at risk for their health. The present formulation supports the continuous milk synthesis, healing of the glandular secretary epithelium and suppresses the growth of microbes. It is highly economical and cost per animal is around Rs 70/- against cost of standard treatment regimens costing more than Rs. 500/- per case and can be adopted by even illiterate and semi-literate farmers, as it is simple, easy to handle and without need for any extra facilities. With longer shelf-life, the technology can restore milk production at much higher rate than in the case of standard treatment. o New color based method for detection of detergent in milk Marine Products o Pond and Cage culture for cobia fish Technology on cage grow out culture of cobia was developed using formulated feed at a stocking density of 4 fish/m3 with the production of 8 kg/m3 with an average weight of 4.5 kg in a period ten months. o Pond grow out technology of cobia fish
  • 13. Agri- Tech Investors Meet 13 Technology on Pond grow out culture of cobia was developed for the first time in India with the production of 1kg/sq. m at the stocking density of 0.25 fish/m2(1fish/4 m2) with an average weight of 4 kg in a period of ten months. o Suitable wet feed and feeding protocol for developing cobia broodstock For cobia culture operations, huge amount of feed is needed. The wet feed technology will ease the culture technology of cobia. This will also help in solving financial commitments of farmers and women SHG as it provides employment opportunity to the fisher folks. o Cobia value added products Technology for making value added products from cobia like cobia pickle , cobia macaroni/noodles, canned cobia, cook chilled cobia fish curry, retort pouch cobia fish curry, fresh /tray packaging, vacuum and modified atmospheric packaged products, collagen and leather. Horticulture o Technologies for development of dry flower products Identification of 40 locally available species suitable for dry flower technology has been done. The process has been refined and technology is ready for commercialization. o Packaging and value addition for jasmine flowers o Precision production technology of marigold o Recovery of lac dye of improved quality o Improved bleaching agent for bleached lac Crop Production o Modular vector for one-step assembly of intron containing hairpin RNA for high throughput gene silencing in plants RNAi mediated gene silencing requires synthesis/introduction of dsRNA in the cells, this requires assembly of intron containing hairpin (IHP) RNA constructs. This is a cumbersome process needing multiple steps of PCR, cloning etc. The current technology highly simplifies the process of ihp assembly and requires just a single steps of PCR, restriction, ligation and transformation, thus minimizing the effort, cost and time required for the ihp assembly. o Anthocyanin enriched tomato fruits Anthocyanins are potent antioxidants and cell cycle regulators proven to have protective functions against cancer and cardio vascular diseases. Dietary consumption of anthocyanins lowers the health risks and WHO estimates that 80% of heart diseases and a third of cancers can be avoided by following healthier diet. Tomato plants were metabolically engineered to accumulate
  • 14. Agri- Tech Investors Meet 14 anthocyanins thought the fruit. The anthocyanin accumulation was 100-150 folds. The carotenoid (lycopene) content was unchanged thus resulting in combined benefits of water soluble anthocyanins and fat soluble carotenoids by consumption of the tomato fruit. o Extraction and production of anthocyanin concentrate from black carrot o Know-how for the production of capsicinoid concentrates from chilli o Extraction and production of lycopene from tomato o Refinement in process for the extraction of steviol glycoside from Stevia rebaudiana o Nutraceutical-enriched guava, grape and mango pana RTS functionalised with black carrot anthocyanins Agri-Inputs o Xylanase hyper-producing thermophilic fungal strain A thermophilic fungal strain has been developed by screening and selection amongst the wild type strains of Thermomyces lanuginosus, isolated from the composting soils of Amritsar. The strain CM11 was developed from this, which produces one of the highest cellulase free xylanase. The xylanase (2800 units/ml) is produced maximally in shake flask culture in a complex medium and the optimization was carried out by response surface methodology using Box- Behnken design of experiments which were validated by repeat experiments. o Organic liquid fertilizer from banana pseudostem sap Banana pseudostem sap is a rich source of plant nutrients like K and plant growth regulators. This can be enriched with organic inputs and when applied to crop plants like mango, chili, brinjal and other crops increases yield by 10-13%. o Nanotechnology for enhanced utilization of native Phosphorous and other elements by plants and higher moisture retention in arid soils Nano-fertilizers are the essential plant nutrients prepared by isolating microorganisms which produce certain enzymes that can break down respective salts to nano-particles readily absorbed by plants through the stomatal route. The nano-fertilizers enhances nutrient supply to crop plants while reduces chemical fertilizer usage, with a three-fold increase in Nutrient Use Efficiency (NUE) and effects cost reduction: per hectare chemical fertilizer usage cost on an average is Rs. 960 for Phosphorous fertilizers and Rs 800 for Zinc based fertilizers while using nano based fertilizer, it is Rs. 300 each for Phosphorous and Zinc based fertilizer. o ELISA Kit (Expression constructs or Serodiagnosis of Plant viruses) for detection of cucumber mosaic virus, potyvirus and Peanut mottle virus
  • 15. Agri- Tech Investors Meet 15 The technology involves serological (protein based) diagnosis of plant viruses. Traditional methodology has a limitation to produce ELISA based plant virus diagnostic kit as antigen preparation is difficult on renewable basis. The following innovations were made for generating antigen and antibody in E. coli, which has prospect for up scaling and commercial utilization: Antigen constructs preparation using full and core conserved region of capsid protein gene sequence that over produced adequate antigen required for polyclonal antibody production. Engineered monoclonal antibody expression constructs that generate antibody directly in E.coli. o Bio-pesticidal nematodes for managing insect pests Bio-pesticidal nematodes isolated from Indian soils which are effective at a temperature range of 15 to 350C. o Samfungicide: Nano-fungicide The present invention relates to the development of surface modified monoclinic sulfur nanoparticles of average size 20-100, their liquid synthesis using polyethylene glycol-400 as a surfactant and use as fungicide. Nanoparticles thus synthesized provide excellent fungitoxic efficacy against the fungal pathogens. Help Desk National Agricultural Innovation Project Room No. 514, Krishi Anusandhan Bhawan - II Pusa Campus, New Delhi - 110 012 Tel: +91-8978855775 Email: aim.naip@icar.org.in Venue of the event AP Shinde Symposium Hall NASC Complex Dev Prakash Shastri Marg, New Delhi 110 012

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