LumaCyte’s RadianceTM instrument can be used for rapid viral infectivity measurements that correlate with viral titers from the standard plaque assay. LumaCyte’s mission is to provide scientists and researchers with transformational research tools that accelerate biological and pharmaceutical research, discovery, and manufacturing through a novel, label-free approach to single-cell analysis and sorting.

1. LumaCyte Technology & Applications:
Rapid Viral Titering

2. Viral Titering
The gold standard method is the
plaque assay:
- Cells are grown in a monolayer
and infected with virus at
varying concentrations
- Clear plaques appear on the
monolayer where cells were
killed by the virus
Researchers perform viral titers to measure the
number of infectious particles in a sample

3. Viral Titering
Despite its usefulness,
the plaque assay is:
- Slow: Requires 5-10 days growth
- Labor intensive
- Low throughput
- Subject to variability and user error

4. Viral Titering
Other methods have limitations:
- TCID50 is slow and often subjective
- Antibody based detection requires
proper antibody selection (if one is
even available), and the associated
expensive and time consuming
staining steps
- PCR requires expensive
reagents and primers must be
made for each new virus

5. A New Approach: RadianceTM
Fast, automated process:
 < 24hrs from infection to results
 10 minutes analysis time per sample
 Correlates directly with plaque assay
 Serial dilution not required
Viral infectivity measurement by
applying virus to cells

6. RadianceTM

7. RadianceTM
Fundamental Technology
o Initiates and measures
optical forces on single
cells
o Precisely characterizes
intrinsic cellular
properties:
• Shape, Morphology
• Biochemistry
• Deformability

8. RadianceTM
o Microfluidic environment
precisely controls cells, enabling
single cell analysis and sorting
o High resolution imaging and
video capture of each cell
o Cells are viable after sorting for
further analysis
o Small volumes can be used to
conserve precious samples
Features

9. RadianceTM
Application for
Infectivity Assays
• Can detect and quantitate viral infection
in mammalian cells
• Is a rapid, automated system for the
detection of viral loads in mammalian
cells
• Generates results that correlate closely
with viral titer from plaque assay
• Is a useful tool for viral vector
research/development and
manufacturing

10. RadianceTM : Who Benefits?
Small, mid-size and global biopharmas that want to
control costs while simultaneously increasing the
speed and accuracy of their research, development,
and manufacturing.

11. Thank you for your time and kind consideration.
Lumacyte.com
(888) 472-9295
info@lumacyte.com