1. DIPAK KUMAR DASH
PRODUCTION OFFICER
MARCK BIOSCIENCE
GOA
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2. DISINFECTION & STERILIZATION

STERILIZATION
Process of
removing microbes
inclusive of
bacterial
endospores.
 DISINFECTION
process of
removing microbes
except bacterial
endospores.
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3. STERILIZATION / DISINFECTION
Physical method
Heat
Radiation
Moist heat
Dry heat
Autoclave
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4. DRY HEAT STERILISATION
Incineration
Over baking

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5. MOIST HEAT STERILISATION
Autoclaving
Boiling

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6. RADIATION
It is of two type that is
1.Ionize radiation
2.Nonionizing radiation
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7. FILTRATION
Membrane filter with pore size
between o.2 to 0.45 micrometer.
Use for heat labile liquid.
Ex-serum, anti biotic sol.

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8. TYPE OF FILTER ARE
1.
2.
3.
4.
5.
EARTHENWARE FILTERS
ASBESTOS FILTER
SINTERED GLASS FILTERS
MEMBRANE FILTERS
AIR FILTERS
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9. VIBRATION
Sound waves of frequency >20,000
cycle/second kills bacteria and some
viruses exposing for one hour.
Use to disinfect instrument.

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10. CHEMICAL STERILISATION



ETHYLENE OXIDE
FORMAL DEHYDE
BETAPROPIOLACTONE
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11. ETHYLENE OXIDE
It is an alkylating agent.
It act by alkylating sulphydryl,amino,
carboxyl groups.
It is highly flammable since it is use in
mixture with carbon dioxide.

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12. USE
It is to sterilize rubber and plastic
material
DISADVANTAGE.
highly toxic,iritate eye

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13. FORMAL DEHYDE
It use to disinfect interiors of the
room.
In high concentration sporicidal.

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14. BETAPROPIOLACTONE
It’s a sporicidal product at a conc. of
1.5 mg/kg.

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15. Disinfectant
Disinfectant are the substance that
are applied to non living objects to
destroy microorganism.
It is less effective than sterilization.

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16. Commonly used
disinfectant
1.
2.
3.
4.
5.
6.
Alcohol
phenol
Chlorhexane
Glutaraldehyde
Povidon-iodin
chloroform
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17. SURFACE
DISINFECTION
Agents that damage bacterial cell
membranes may be used to disinfect
inert surfaces or skin.
Ex
detergents,alcohol

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18. CONCLUSION


All sterilization processes must be
validated.
These process should be care fully
done for effective product.
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19. REFERENCES
Sharma P.P; How to practice GMPs; (Good Manufacturing Practices); Fourth
Edition ; Vandana Publication pvt. Ltd. Delhi. pp-208-280

Potdar M.A ; cGMP; (Current Good Manufacturing Practices) For
Pharmaceuticals; Pharma Med Press; Hyderabad-500095. pp-339-384

Shah D.H.; SOP Guidelines; straight and simple; 1 st edition; published by
Business Horizons;G-59, New Delhi-110048, pp-161-183.

Mallik V; Drugs& Cosmetics Act 1940; 18th edition;2005; published by
Eastern Book Company; Lucknow.

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