Sterilization and disinfection

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Sterilization and disinfection

  1. 1. DIPAK KUMAR DASH PRODUCTION OFFICER MARCK BIOSCIENCE GOA 1
  2. 2. DISINFECTION & STERILIZATION  STERILIZATION Process of removing microbes inclusive of bacterial endospores.  DISINFECTION process of removing microbes except bacterial endospores. 2
  3. 3. STERILIZATION / DISINFECTION Physical method Heat Radiation Moist heat Dry heat Autoclave 3
  4. 4. DRY HEAT STERILISATION Incineration Over baking  4
  5. 5. MOIST HEAT STERILISATION Autoclaving Boiling  5
  6. 6. RADIATION It is of two type that is 1.Ionize radiation 2.Nonionizing radiation 6
  7. 7. FILTRATION Membrane filter with pore size between o.2 to 0.45 micrometer. Use for heat labile liquid. Ex-serum, anti biotic sol.  7
  8. 8. TYPE OF FILTER ARE 1. 2. 3. 4. 5. EARTHENWARE FILTERS ASBESTOS FILTER SINTERED GLASS FILTERS MEMBRANE FILTERS AIR FILTERS 8
  9. 9. VIBRATION Sound waves of frequency >20,000 cycle/second kills bacteria and some viruses exposing for one hour. Use to disinfect instrument.  9
  10. 10. CHEMICAL STERILISATION    ETHYLENE OXIDE FORMAL DEHYDE BETAPROPIOLACTONE 10
  11. 11. ETHYLENE OXIDE It is an alkylating agent. It act by alkylating sulphydryl,amino, carboxyl groups. It is highly flammable since it is use in mixture with carbon dioxide.  11
  12. 12. USE It is to sterilize rubber and plastic material DISADVANTAGE. highly toxic,iritate eye  12
  13. 13. FORMAL DEHYDE It use to disinfect interiors of the room. In high concentration sporicidal.  13
  14. 14. BETAPROPIOLACTONE It’s a sporicidal product at a conc. of 1.5 mg/kg.  14
  15. 15. Disinfectant Disinfectant are the substance that are applied to non living objects to destroy microorganism. It is less effective than sterilization.  15
  16. 16. Commonly used disinfectant 1. 2. 3. 4. 5. 6. Alcohol phenol Chlorhexane Glutaraldehyde Povidon-iodin chloroform 16
  17. 17. SURFACE DISINFECTION Agents that damage bacterial cell membranes may be used to disinfect inert surfaces or skin. Ex detergents,alcohol  17
  18. 18. CONCLUSION   All sterilization processes must be validated. These process should be care fully done for effective product. 18
  19. 19. REFERENCES Sharma P.P; How to practice GMPs; (Good Manufacturing Practices); Fourth Edition ; Vandana Publication pvt. Ltd. Delhi. pp-208-280  Potdar M.A ; cGMP; (Current Good Manufacturing Practices) For Pharmaceuticals; Pharma Med Press; Hyderabad-500095. pp-339-384  Shah D.H.; SOP Guidelines; straight and simple; 1 st edition; published by Business Horizons;G-59, New Delhi-110048, pp-161-183.  Mallik V; Drugs& Cosmetics Act 1940; 18th edition;2005; published by Eastern Book Company; Lucknow.  19
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