By: El-­‐Sayed A. El-­‐Absawy, Amal A. Abdelaziz, and Samah M. Eldemery* Corresponding author: samah2004eg@yahoo.com

1. SRAP and TRAP Markers for Heat Stress Tolerance in Wheat (Triticum aestivum)
Samah M. Eldemery 1, Amal A. Abdelaziz 1, El-Sayed A. El-Absawy2 *
1 Molecular Biology Department, GEBRI, Sadat City, Minoufiya University, Egypt
2 Bioinformatics Department, GEBRI, Sadat City, Minoufiya University, Egypt
*Corresponding author: samah2004eg@yahoo.com 2012
Four hexaploid wheat varieties (two Egyptian – ‘Giza 168’ and ‘Gemmiza 7’, one Mexican heat tolerant variety –
‘SERI 82’, and one heat susceptible Chinese variety – ‘Chinese Spring’) were used to characterize and analyze the
genetic differentiation of heat tolerance in wheat by both SRAP and TRAP markers. 25 SRAP primers combinations
and 12 TRAP primers combinations were used. The polymorphic information content (PIC) of TRAP markers was
higher than the PIC of SRAP markers, 81.8% and 59% respectively. Different SRAP and/or TRAP markers have
been assigned to the genotypes and were correlated with the heat tolerance or susceptibility. Many markers were
assigned to ‘Chinese Spring’ genotype, and they were proposed to be correlated with the heat susceptibility in
wheat. The results of both SRAP and TRAP dendrogram showed the same results and separated the four wheat
genotypes into two distinct clusters, one containing genotypes ‘Giza 168’, ‘Gemmiza 7’, and ‘SIRI 82’, and one
containing the ‘Chinese Spring’ genotype. The study concluded that SRAP and/or TRAP markers can be used
efficiently in characterization studies and in differentiation of heat tolerant wheat genotypes.
Key words: Wheat, SRAP, TRAP, heat tolerance, genetic diversity
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SRAP cluster analysis of four wheat TRAP cluster analysis of four wheat
genotypes depending upon simple genotypes depending upon simple matching
matching coefficient and using the coefficient and using the UPGMA clustering
PCR products patterns of 25 PCR products patterns of 12 TRAP
UPGMA clustering method. method.
SRAP primers combinations on primers combinations on four
four wheat genotypes differed in wheat genotypes differed in heat
heat tolerance response. tolerance response.