User Guide: Pulsar™ Weather Station (Columbia Weather Systems)
Technologies to drive maize yield improvement
1. Agriculture Division of DowDuPont™
Corteva AgriscienceTM - All rights reserved1
Technologies to drive maize yield
improvement
Neal Gutterson
Chief Technology Officer
13th Asian Maize Conference and Expert Consultation on
“Maize for Food, Feed, Nutrition and Environmental Security”
Ludhiana, India
October 8th-10th, 2018
2. Agriculture Division of DowDuPont™ Corteva AgrisciencesTM - All rights reserved
Our Purpose: To enrich the lives of who produce and those who consume,
ensuring progress for generations to come
3. Agriculture Division of DowDuPont™ Corteva AgrisciencesTM - All rights reserved
Corteva AgriscienceTM by the Numbers
people strong
22,000
countries
130+
crops
10+
active ingredients
65+
EMEA
~20%
Asia Pacific
~10%
North America
~50%
Latin America
~20%
seeds
crop protection
$14B
in sales*
* Excludes revenue associated with remedies
4. Agriculture Division of DowDuPont™ Corteva AgriscienceTM - All rights reserved 4
Providing Complete Solutions for farmers
Offering farmers superior solutions, expanded choice and greater value
Delivered through customer-centric sales channels
Solving customer challenges with a broader
product and service offering
World-class unified R&D capabilities driving
agricultural innovation, promising new technologies
and a robust pipeline
DIGITAL
BREEDING
DIGITAL
SOLUTIONS
BIOTECH
SEED APPLIED &
BIOLOGICAL
TECHNOLOGY
CROP
PROTECTION
AGRONOMIC
SOLUTIONS
TARGETED
BREEDING
Seed Crop Protection
5. Agriculture Division of DowDuPont™
Corteva AgriscienceTM R&D Footprint in APAC
Plant Breeding
Integrated Field Sciences
Regulatory Sciences
Trait Development
Data Science and Informatics
6. Agriculture Division of DowDuPont™ Corteva AgriscienceTM - All rights reserved
Research Operations
Technology Deployment
Rice
Product Development
Maize
Product Development
Millet
Product Development
Mustard
Product Development
Research Competencies in Corteva Plant Breeding in APAC
Marker
Technology Lab
Double Haploids
Production Lab
Rice Grain
quality lab
Pathology
lab
Mechanization
support
Targeted Drought
Research
NRSP & Inbred
Management
Molecular
Breeding
Facilities and
Operations
Data Science and
Informatics
Precision
Phenotyping
Native Traits
Introgression
7. Agriculture Division of DowDuPont™ Corteva AgrisciencesTM - All rights reserved 7
Advanced Phenotyping
Systems
Digital Solutions
Genome Editing via
CRISPR-Cas9
Germplasm insight,
genomics
Driving Maize improvement through new technologies
8. Agriculture Division of DowDuPont™ Corteva AgriscienceTM - All rights reserved
Advanced Phenotyping
Systems
Examples: Plant
height, disease
• Advanced phenotyping deployed broadly in regions and
crops
• Enabled by data analytics, AI
• Developed internally and through collaborations
9. Agriculture Division of DowDuPont™ Corteva AgriscienceTM - All rights reserved
Specialized Phenotyping
Managed Env.
UAS Imaging
Exp. Design
Ear Photometry
Disease Screens
Standability
Specialized & real time phenotyping
Advanced engineering to scale phenotyping
Advanced Phenotyping Frameworks
Cross-functional
teams of:
- Plant Breeders
- Computer scientists
- Engineers
- Data Scientists
- Statisticians
10. Agriculture Division of DowDuPont™ Corteva AgriscienceTM - All rights reserved 10
Soy Maturity
Time
Greenness
**
f(x)
Foliar Disease
UAS Remote Sensing Plant
Height
DJI Matrice 600DJI Phantom 4 Pro
Early Stand & Vigor Field Variability
Plant Count
Scouting
11. Agriculture Division of DowDuPont™ Corteva AgriscienceTM - All rights reserved 11
Digital Solutions
Example:
Encirca Pro
Imagery
• Capitalizes on satellite imagery and data analytics
• Monitors crop health down to a 5-meter resolution
• Refreshes every 3-10 days, depending on cloud cover
• Provides crop health alerts and directed scouting
• Enables precise, real-time on-the-farm decisions
12. Agriculture Division of DowDuPont™ Corteva AgriscienceTM - All rights reserved
Nebraska Nitrogen Stabilization Trial 2018 In-Season Evaluation
• Comparison of plots with
and without Instinct HLTM
on same day
• Real-time crop-health
monitoring via satellite
remote sensing, delivered
via digital mobile platform.
• Differences in plant health
clearly evident (June 15,
2018)
13. Agriculture Division of DowDuPont™ Corteva AgriscienceTM - All rights reserved 13
Germplasm Insight,
Genomics
Example:
Inbred reference
genome quality
• Latest sequencing technologies integrated to best-in-
class reference genome capability
• High quality reference genomes, haplotype pedigrees
14. Agriculture Division of DowDuPont™ Corteva AgriscienceTM - All rights reserved
Key infrastructure: One
Illumina Novaseq 6000, One
Illumina HiSeq 4000, three
Illumina HiSeq 2500, three
NextSeq, and eight MiSeq
sequencing instruments and
supporting automation systems
Corteva Genomics 2018 core infrastructure
ONT MinIon & PromethIon Bionano Saphyr
PacBio Sequel x 3
Illumina sequencing platform: Short-read technology (50-250bp)
Single-molecule, Long-read technologies (10,000bp+)
15. Agriculture Division of DowDuPont™ Corteva AgrisciencesTM - All rights reserved
Corteva advances in chromosome reconstruction
15
1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10 1 2 3 4 5 6 7 8 9 10
2016* 2017 2018300
200
100
0
Length(Mbp)
504 scaffolds 101 scaffolds 24 scaffolds
CSB8V
Data collection: 9 days
Cost: $35,000
GR84Z
Data collection: 21 days
Cost: $55,000
ED85E
Data collection: 180 days
Cost: $142,000
16. Agriculture Division of DowDuPont™ Corteva AgrisciencesTM - All rights reserved
P3511
P3592
P3388
P3552
30 industry leading products in INDIA in 15 years
Kharif
Professional
Team
Industry
Yield & Market
Leader
Prediction
Success
Best Germplasm
Strong
Processes
& Testing
2003 2004 2005 2007 2009 2010 2011 20132012
P3401
30V92 30B07 30R77 P3785 3501 P3396 P1864 P337730B11
P3540
P3502 P3441
P3400
P353331Y45
P3522 P3542P3546
P3550P3580
Rabi
All Season
2014 2015 2016 2017
P1844
P3377
P3399
P3535
P3355
P1855
P1866
• More Food for Growing Population –
• 9% yield increase from 2002-2015
• Pioneer has 22% market share with reliable brand image with farmer in
Corn
• More Farmer Income –
• DuPont Pioneer corn hybrids generated more income per unit area
• Market leading products in millet, mustard and rice
The Impact
17. Agriculture Division of DowDuPont™ Corteva AgriscienceTM - All rights reserved 17
Germplasm, Traits & Seed
Applied Technology
Example:
Gene Editing
Including
CRISPR-Cas
Potential for:
• higher yield on less land
• less waste through reduced browning
• Improved tolerance to pests and disease caused by climate warming
• Ability to tackle citrus greening and other pathogens
18. Agriculture Division of DowDuPont™
Required Competencies for CRISPR-CasTargeted Breeding
18
Understanding Elite Genetics
High quality DNA sequencing
Informatics tools and infrastructure
Advancing CRISPR-Cas Technology
Tool with superior activity and targeting specificity
Incorporating in-house & collaborators’ expertise
Delivery into Elite Genetics
Ability to directly introduce targeted
improvements to already high-quality varieties
21. Agriculture Division of DowDuPont™
Transgenic trait development
txn stage C stage D stage E stage F
Year 13
1st commercial
planting
Year 1 Year 13Year 11Year 9Year 7Year 5Year 3
Year 5
1st commercial planting
Next Generation Waxy corn
edit test R4-R5
event
characterization
& selection
advanced testing
& regulatory
submissions
pre-launch
Edited variety development can be very efficient
TC1/2 R1-R5 testing
Traditional plant breeding
Year 8
1st commercial
planting
DH
23. Agriculture Division of DowDuPont™
Technical approach to waxy product development
23
100% conserved GuideRNAs from NGS allele models
• Particle Gun >> whole gene deletion(s)
• 4kb D – Reg Roadmap and Lead Product Concept
• 6kb D – Alternative Product Concept
• Recapitulate in ~1doz. elite inbreds (1yr !)
• Quality variants for field testing / commercialization
• Target: BC1F2 generation from CE > NRSP (F2016)
24. Agriculture Division of DowDuPont™ Corteva AgriscienceTM - All rights reserved
4kb deletion junction sequence alignment
Molecular Advancement - NGS
25. Agriculture Division of DowDuPont™ Corteva AgriscienceTM - All rights reserved
Next gen waxy performance
Non-waxy Conv. waxy Next Gen waxy * indicates significance; p value <0.05
+ indicates significance; p value <0.1
Yield advantage of Next Gen Waxy Vs TI-Waxy
Plans: 2019 commercial pilot; 2020 commercial launch
26. Agriculture Division of DowDuPont™ Corteva AgriscienceTM - All rights reserved 26
Beyond waxy with targeted breeding through CRISPR
Broadest CRISPR patent estate in the agriculture industry – leading the global dialogue
Broad agricultural applications of CRISPR Near-term product to market
Disease
resistance
Yield &
Yield
Stability
Drought
Tolerance
Output
Traits
Maturity
Corn
Soybean
Canola
Rice
Wheat
Sunflower
Next product in pipeline focused
on oil quality
First commercial agricultural product
Waxy corn hybrids
Addressing healthier oil demand – and
processor needs for more stable oil
High oleic – low linoleic soybean
27. Agriculture Division of DowDuPont™ Corteva AgriscienceTM - All rights reserved
Partnering: To enrich the lives of who produce and those who consume,
ensuring progress for generations to come
28. Agriculture Division of DowDuPont™ Corteva AgriscienceTM - All rights reserved 28
Sparking New Solutions Through Open Innovation
29. Agriculture Division of DowDuPont™ Corteva AgrisciencesTM - All rights reserved
Collaborating to assure broad technology adoption
Sorghum and millet improvement
with ICRISATCIMMYT & Corteva addressing MLN
Cassava and sorghum
improvement
with Danforth Center
31. Agriculture Division of DowDuPont™ Corteva AgrisciencesTM - All rights reserved
Supporting graduate programs, research centers and meetings
The Plant Sciences Symposia Series 2008-2018
Wageningen University
University of Hohenheim
AgroParisTech/Paris-Sud
University of Edinburgh
University of Minnesota
University of Wisconsin
Cornell University
University of California Davis
University of Nebraska
Iowa State University
Texas A&M University
Purdue University
University of Illinois
University of California Berkeley
University of Florida
Colorado State University
University of Georgia
Washington State University
University of Missouri
University of Guelph
CIMMYT
American Society of Agronomy
Sorghum ICNA
University of Hawaii
University of Manitoba
The Ohio State University
Clemson University
North-West University
University of Pretoria
SAPB Symposium
University of Queensland/TropAg
Huazhong Ag University
Northwest AAF University
Shandong Ag University
Sichuan Ag University
Henan Ag University
JAAS, Changchun
CIAT
Federal University of Viçosa
Federal University of Lavras
University of Sao Paolo Piracicaba
Federal University of Brasilia
Brazilian Plant Breeding Congress
University of Norte Fulminense
Embrapa Arroz e Feijao
Punjab Agriculture
University
University of Bangalore
PJT State Ag University
IARI
ICRISAT
IRRI
Since 2008: >128 events hosted by >50 institutions representing 13
countries on 6 continents; >20,000 participants
Editor's Notes
To enrich the lives of those who produce and those who consume, ensuring progress for generations to come
Our collective strength as the DowDuPont Agriculture division will be aligned around a common Purpose. This is: “To enrich the lives of those who produce and those who consume, ensuring progress for generations to come.” [PAUSE]
Our Purpose is our overall reason for being. It’s the basic reason we do what we do – WHY we come to work every day. It will function as our north star - that will orient us as we grow our business and engage in the world over the long term.
Our purpose is expansive – it distinguishes us from our competitors and commits us to mission which is ambitious and aspirational.
[NEXT SLIDE]
Together, these digital solutions and the digital expertise we are building across our business space help to support a broader transition toward our goal of shaping a more connected, sustainable agriculture industry.
By partnering with customers and consumers, we are focused on engaging in the conversation around the continuously evolving nutritional needs and preferences that are driving innovation and change across the food value chain, while at the same time innovating with our customers and partnering with society to address the most pressing challenges facing our industry and those served by it.
Here, we remain focused on responsible stewardship – integrating sustainability criteria early in the product development phase to lower the environmental impact of our product portfolio over time, while also leveraging the power of digital to enhance the stewardship of these existing and emerging portfolios.
Facilitator Note:
Improving throughput and accuracy of plant phenotyping is core to continued advances in breeding to ensure genetic gain to meet global food demand. Current manual phenotyping requires enormous investments in time, cost, and labor as quantitative values are required for thousands of genetic varieties across different environments presents a huge challenge in scalability. Low cost unmanned aerial systems (UAS) have a great potential for rapid and high-throughput phenotypic plant measurements. Coreva Agriscience has developed, validated and deployed a suite of high-throughput phenotyping solutions ranging from plant counting and early stand quality assessments, to quantitative assessments of soy maturity, plant height and foliar disease across 7 crop species. Moreover, we have developed and deployed field solutions for scouting and real-time assessment of crop establishment and field variability. These solutions are driven by on a large fleet of UAS, a dedicated cadre of hundreds of UAS pilots and image analysts and top level remote sensing, computer vision and full stack software engineering specialists within R&D.
Talking points: (regulatory roadmap)
Known gene and well studied
Current market segment
Products are identity-preserved
Addressable product challenges
2-3 yr time lags compared to platform hybrids (tier 3 conversions)
3-5% yield penalty
Source of waxy variation originated from non-elite corn variety
Sequences near CR4 and CR8 are conserved in all 12 lines
Sequences near CR10 are conserved in 9 lines, they are the same as in GR2HT
Sequences near CR10 in HN8SY and EHM3 are the same, but slightly different from GR2HT and other 8 lines; Sequence near CR10 in ED85E has 1nt difference from the HN8SY and EHM3. Different nts are in orange font.
Only the advanced variants’ junction sequences are presented here
6kb deletion junction sequences have few versions (6 in total).
Talking points:
Met or exceeded product delivery expectation
Delivered equivalent amylopectin levels, i.e. functionality
Delivered on overall product performance
On-track for 2019 launch
As we look to the future…. Industry-leading positions.
Our collective strength as the DowDuPont Agriculture division will be aligned around a common Purpose. This is: “To enrich the lives of those who produce and those who consume, ensuring progress for generations to come.” [PAUSE]
Our Purpose is our overall reason for being. It’s the basic reason we do what we do – WHY we come to work every day. It will function as our north star - that will orient us as we grow our business and engage in the world over the long term.
Our purpose is expansive – it distinguishes us from our competitors and commits us to mission which is ambitious and aspirational.
[NEXT SLIDE]