Measuring Glucose in Potatoes Using Permanganate Titration
1. Quantifying the Amount of Glucose in a Potato Beryllium JOSE, Williard DAROYA, Rangel UTANES, Kerwin ELATICO, Adam TANCHULING, Diego MAGNO, Gian
2. Intorduction There are a lot of ways to determine the glucose concentration of a solution… These include the use of special indicators, titration, or osmosis But for this particular experiment, a different protocol has been used.
4. Preparation of Solutions 100mL of 1 M of sulfuric acid 125 mL of 0.0025316 M of Potassium permanganate Glucose solution (1%, 2%, 4%, 8%) Potato Solution (approximately 0.5 g of crushed potato in 50mL of water)
5. Preparation of Solutions Fig 1. Adding sulfuric acid in distilled water. Fig 2. Stirred potassium permanganate in distilled water.
6. Preparation of Solutions Fig 3. Weighing of solid glucose to be used in solutions Fig 4. Adding distilled water to solid glucose.
7. Preparation of Solutions Fig 5. Weighing of potato slices. Fig 6. Three of approximately 0.5 grams of potato slices.
8. Preparation of Solutions Fig 7. Crushing 0.5 grams of potato slice using mortar and pestle. Fig 8. Placing crushed potato in Erlenmeyer flask.
9. Preparation of Solutions Fig 10. Three potato solutions, four glucose solutions, and a potassium permanganate solution Fig 9. Adding distilled water to crushed potato.
10. Glucose and Potato Solutions Five milliliters of sulfric acid was added using syringe After adding sulfuric acid, two milliliters of potassium permanganate was added (syringe) Time was recorded right after potassium permanganate was added until color disappears from solution
11. Adding Sulfuric Acid Fig 11. Adding sulfuric acid to glucose solution. Fig 12. Adding sulfuric acid to potato solution.