Participants of the workshop learn the necessary background information and techniques to diagnose Sars-CoV-2 using the mobile diagnostic laboratory. The laboratory is shipped ready to use with all devices, reagents, certificates, and protocols. After one day of preparation together with a local assistant, a five-day course is given where every step is carried out by each participant. Experts accompany the learning process with written teaching materials, video training, virtual live coaching, and short exams to verify the learned content.

1. MOBILE CORONA DIAGNOSTIC LAB
THE PRINCIPLES OF
PCR, RT-QPCR AND RT-LAMP

2. ABOUT MYSELF
July 8th, 2021
MOBILE CORONA DIAGNOSTIC LAB | Diagnostic Methods and Data Management slide 2
M.Sc. in biochemistry (Technical University Munich)
PhD in Experimental Medicine (Helmholtz Zentrum
Munich)
Susanne Pettinger, M.Sc.
contact: susannepettinger@gmail.com
MY EXPERTISE
• Cell culture | microbiological cultures
• Molecular biology | biochemistry | different assays
• Protein expression and purification | protein engineering
• Electron microscopy | fluorescent imaging

3. Block 1:The PCR method
• How does PCR work?
• Reverse transcription
Block 2: RT-qPCR
• Why qPCR?
• Real-time detection methods
• RT-qPCR in the Mobile Lab
BREAK
Block 3: RT-LAMP
• Introduction
• How does LAMP work?
o LAMP primers
o The LAMP reaction cycle
• Detecting LAMP products
• RT-LAMP in the Mobile Lab
July 8th, 2021
MOBILE CORONA DIAGNOSTIC LAB | Diagnostic Methods and Data Management
SCHEDULE

4. BLOCK 1:THE PCR METHOD

5. PCR – A GAME CHANGER FOR SCIENCES
July 8th, 2021
MOBILE CORONA DIAGNOSTIC LAB | Diagnostic Methods and Data Management
• Polymerase Chain Reaction allows the amplification of DNA from a scarce sample
• The invention of PCR had a huge impact on
o Research: cloning, sequencing, measurement of gene expression, ….
o Medicine: identification of pathogens, typing tissue and blood, diagnosis of genetic defects
o Forensics: DNA comparison of blood or hair samples, parentage testing
o Paleontology: amplify and sequence DNA from trace amounts in fossils millions of years old

6. THE DISCOVERY OF PCR – A PARADIGM SHIFT IN THREE NOBEL PRIZES
July 8th, 2021
MOBILE CORONA DIAGNOSTIC LAB | Diagnostic Methods and Data Management

7. HOW DOES PCR WORK?
July 8th, 2021
MOBILE CORONA DIAGNOSTIC LAB | Diagnostic Methods and Data Management
• DNA template
• DNA polymerase
• complementary primers
• dNTPs
• Magnesium (Mg2+)
• buffer

8. REVERSE TRANSCRIPTION: EXPANDING PCR TO THE RNAWORLD
July 8th, 2021
MOBILE CORONA DIAGNOSTIC LAB | Diagnostic Methods and Data Management
• DNA polymerases can only bind double-stranded DNA → RNA templates have to be transcribed to cDNA
• Reverse transcriptase: enzyme originating in retroviruses