Zagro's Gut Bio is a synbiotic supplement composing of stable probiotics and prebiotics, used for the prevention and treatment of enteric diseases and promotes the gut health.

1. GUT BIO
For GUT HEALTH

2. ✓ Gut pathogenic microrganisms are
ultimately the cause of gastrointestinal
infections in animals which reduce
intestinal health and contribute to
reduced animal performance.
✓ Even in the absence of disease, both
opportunistic and overtly pathogenic
microrganisms can be isolated from the
gastrointestinal tract .
GUT Pathogens

3. Enteric diseases are major economic concern
in the poultry/animal industry.
➢ Affecte the productivity
➢ Affecte gut health
➢ Increase the mortality
➢ Bacterial contamination of animal meat
Impacts

4. Major Bacterial Diseases
Caused by Diseases
E.Coli Colibacillosis
Salmonella
spp. including
pullorum and
gallinarum
Salmonellosis, Enteritis
Clostridium sps
Necrotic Enteritis (NE),
Botulism

5. ➢ The use of antibiotic growth promoters (AGP) to enhance the
efficiency of agricultural animals has been practiced for over 50
years
➢ The types of antibiotics used as growth promoters include
tetracyclines, penicillins, sulfonamides, and aminoglycosides
➢ The action of AGP’s takes place in the gut of animal where they alter
the micro flora environment.
➢ When antibiotics are added to animal feed for prolonged periods of
time, populations of pathogenic bacterial flora develop resistance to
that antibiotic and can become dominant in the gut
Antibiotic Growth Promoters (AGPs)

6. ➢ Antibiotic-resistant bacteria
➢ Affect the gut normal flora
➢ Residues problem related to human health
Disadvantages of Antibiotics Usage

7. Increased gut health = Reduced pathogenic load
Micro flora balance in Chicken GIT

8. ➢ Common terms for Probiotics are friendly,
beneficial or healthy bacteria.
➢ Probiotics are live, nonpathogenic bacteria
that contribute to the health and balance
of the intestinal tract.
➢ Given orally to animal for help to prevent
the intestinal disease
Probiotics

9. 1. Competition with pathogenic bacteria for space,
intestinal attachment sites and nutrients
2. Change of environmental conditions in the
intestine (Lowering of pH through increased
production of volatile fatty acids (VFA) and lactic
acid)
3. Production of antimicrobial substances
(lactoferrin, lysozyme, bacteriocins... "Natural
antibiotics“)
4. Modulation of intestinal immune response
Probiotics

10. ➢ Acid resistant
➢ Antimicrobial activity
➢ Beneficial effect and not cause any harmful
effects to animals
➢ Bile salt tolerant
➢ GI tract colonization properties
➢ To produce metabolites and enzyme
➢ Good viable strain
Characteristics of Good Probiotics

11. 1. Should be a strain which is capable of
exerting a beneficial effect on the host
animal, e.g. increase the growth
2. Should be non-pathogenic and non-
toxic
3. Should be present as viable cells,
preferably in large numbers
4. Should be capable of surviving and
metabolizing in the gut environment, e.g.
resistant to low pH and organic acids
5. Should be stable and capable of
remaining viable for long periods under
storage and field conditions
Good Probiotics

12. ✓ Probiotics, competitive exclusion and direct
fed microbial feed /water supplements can
be used as a strategic tool for managing
beneficial microbial populations.
✓ Applied to farm animal nutrition to improve
feed conversion and increase the Weight
gains
✓ Functional digestive system development
in young animals.
✓ Inhibition effect against pathogens, their
optimizing influence on digestive
processes, their stimulating effect on the
immune system
Probiotics in Animals

13. ✓ Non-digestible food ingredients
✓ Beneficially affect the host by selectively
stimulating the growth and activity of one
or a limited number of bacteria in the colon.
✓ Nutrients to beneficial microbes
✓ Tricking pathogenic bacteria into attaching
to the oligosaccharide rather than to the
intestinal mucosa.
✓ Reduced the intestinal pathogenic bacteria
colonization thereby decreasing the
incidence of infection in the birds.
Prebiotics

14. Antibiotics vs Probiotics vs Prebiotics
Antibiotics Probiotics Prebiotics
Antibiotics are agents
produced by
microorganisms (bacteria,
virus, fungi or destroy
them)
Probiotics are live microbial
feed supplements that
beneficially affect the host
animal by improving its
microbial balance.
Prebiotics are non-digestible
food ingredients that
beneficially affect the host by
selectively stimulating the
growth/or activity of a limited
number of bacteria in the
colon / intestine and thus
improve host health.
Pure chemical compound
synthetic in origin or
produced from bacteria or
fungi.
Living microorganisms which
are singly or combination of
friendly bacteria /yeast.
Non-digestible feeding
ingredients, i.e., chemical
compounds like
oligosaccharides.
Absorbed in GI tract. Not absorbed in GT tract. Not absorbed in GT tract

15. Antibiotics Probiotics Prebiotics
Tissue residues remain in animal
product which may be harmful
to consumers.
No tissue residues No tissue residues
May cause mutation of other
microorganisms and thus
leading to resistant strains
Cause no mutation of
microorganisms
Cause no mutation of microorganisms.
Blocks living cells
(microorganisms) DNA, RNA or
protein synthesis.
Produce acid, reduce pH
and discourage growth of
pathogenic
microorganisms
It leads to production of butyrate and
other SCFA which are quickly absorbed and
can serve as an energy surce for host.
Broad spectrum of activity. Possess localized
antimicrobial activity.
Control lower gut environment,
metabolism and disease prevention.
Use in feed in sub-therapeutic
level and shows growth
promotion of action.
Proliferate in the digestive
tract and compete with
the pathogenic bacteria.
Act as anticarcinogenic, antimicrobial,
hypolipidemic and glucose modulatory
activities improve mineral absorption and
balance and antisteoprotic activity.
Antibiotics vs Probiotics vs Prebiotics

16. ❑ Potentially reduce enteric diseases in
animals and also enhance their
productivity.
❑ These substances have been proposed to
assist in the prevention of carcass
contamination and improve the immune
response.
Probiotics + Prebiotics (synbiotic)

17. GUTBIO WSP
Gut Promoting Supplements

18. ❑ Zagro Gut bio is symbiotic combination
of multi species of beneficial microbes
with two Prebiotics compounds such as
FOS and MOS for prevent and treatment
of enteric diseases and promotes the gut
health
❑ Maintain a healthy gut flora.
❑ Good alternatives of antibiotics
Gut Bio WSP

19. ❑ Improves the villi height
❑ Improves the feed utilization
❑ Improves the nutrient absorption
❑ Increase the growth rate
❑ Neutralize the bacterial toxin
❑ Prevents colonized pathogenic microbes in
GI tract under stress condition
❑ Reduces the GI tract pH
❑ Reduces the Mortality
❑ Reduces Food borne pathogens in animal
products
Benefits

20. Beneficial Bacteria Function
Enterococcus faecium Immune response and better treatment of Escherichia coli
,Salmonella gallinarum, Listeria infection
Lactobacillus
acidophilus
Inhibit the pathogenic bacteria and Production of niacin, folic
acid and pyridoxine; Improve GIT Function ;Boost the immune
system: provide relief from indigestion and diarrhea
Bifidobacterium animalis Reduce the colonization of S. enteritidis or C. jejuni
Lactobacillus salivarius Inhibitory to Campylobacter jejuni
Lactobacillus reuteri immune response in the gastrointestinal
Bacillus sutilis Reduce the necrotic enteritis caused by Clostridium
perfringens
Function of GUT BIO microbes

21. Broiler Performance
Results

22. Parameters
Results in first week of
performance
Gut Bio WSP
Feed Intake g 199
Body Weight 225
FCR 1.07
Mortality % 0.47
Control (without any
probiotic)
Feed Intake g 197
Body Weight 218
FCR 1.09
Mortality % 1.27
Broilers performance report (0-7days )

23. Applications schedules 0 to 7, 10 to 12, 20 to 22,30 to 32days
Gut Bio WSP
Parameters Results
Feed intake g 3388
Avg.wt. kg 2.247
Day Gain g 60
FCR 1.50
Mort % 5.3
EEF 376
Control (without probiotics)
Feed intake g 3402
Avg.wt. kg 2.165
Day Gain g 58
FCR 1.56
Mort % 5.5
EEF 348
Broilers performance report (6th week)

24. Microbial counts in Chicken GIT
Name of the Sample Total Lactic acid
bacillus counts CFU/g
of gut digesta
Total Gram negative
counts CFU/g of gut
digesta
Digesta from gut bio group 3x10 10 5x10 6
Digesta from Control group 5x10 8 2.3x10 7

25. ✓ Animal under Stress condition
✓ Newly hatched chicks and young animals
✓ Intestinal disorder and Feed changeover
time
✓ Loss of appetite
Best Recommendation

26. For Drinking Water
Poultry /Swine
Prevention: Dissolve 20-40 g per 100 L of water for 2-3 days
Treatment: Dissolve 50 to 100 g per 100 L of water for 3-5 days
Cattle/Sheep: 2 to 4g per head per day
For Feed
Poultry : Mix 100 to 250g per ton of feed
Swine : Mix 250 to 500 g per ton of feed
Fish/Shrimp : Mix 1-2 kgs per ton of feed
Dosage & Administration

27. Gut Bio is available in the
following pack sizes:
100G, 500G & 1KG
Packing Size

28. ACCREDITATIONS
Zagro has been awarded the following
certifications issued by DNV-GL:
➢ ISO 9001:2015 - Quality Management
System
➢ ISO 22000:2005 – Food Safety
Management System
➢ FAMI-QS – Feed Safety Management
System
➢ HACCP - Internationally recognized
system for Food Safety
➢ GMP – Good Manufacturing Practices on
Food Hygiene

29. GUTBIO
For HEALTHY GUT
Visit www.zagro.com.