Yu-Wen Li has over 20 years of experience in pharmaceutical drug discovery research as a scientific team leader. They have successfully supported multiple early and late stage drug programs for psychiatric diseases, neurodegenerative diseases, neuropathic pain, and heart failure. Their expertise includes leading research teams to validate drug targets, understand mechanisms of action, and characterize pharmacokinetic-pharmacodynamic relationships using in vitro, ex vivo, and in vivo techniques. They have authored over 40 papers since joining the pharmaceutical industry, with 12 as first or senior author. Currently, they are a Senior Principal Scientist at Bristol-Myers Squibb leading a core group researching genetically-defined diseases.
Acupressure, reflexology, and auricular acupressure for insomnia: a systemati...RONSA1
Previous randomized controlled trials (RCTs) have shown that acupuncture may be efficacious for insomnia. Instead of needling, acupressure, reflexology, and auricular acupressure are procedures involving physical pressure on acupoints or reflex areas. These variants of acupuncture are gaining popularity, perhaps due to their non-invasive nature. A systematic review has therefore been conducted to examine their efficacy and safety for insomnia. Two independent researchers searched five English and 10 Chinese databases from inception to May 2010. Forty RCTs were identified for analysis. Only 10 studies used sham controls, four used double-blind design, nine studies scored three or more by the Jadad scale, and all had at least one domain with high risk of bias. Meta-analyses of the moderate-quality RCTs found that acupressure as monotherapy fared marginally better than sham control. Studies that compared auricular acupressure and sham control showed equivocal results. It was also found that acupressure, reflexology, or auricular acupressure as monotherapy or combined with routine care was significantly more efficacious than routine care or no treatment. Owing to the methodological limitations of the studies and equivocal results, the current evidence does not allow a clear conclusion on the benefits of acupressure, reflexology, and auricular acupressure for insomnia.
Prehospital rapid sequence intubation improves functional outcome for patient...Emergency Live
In adults with severe TBI, prehospital rapid sequence intubation by paramedics increases the rate of favorable neurologic outcome at 6 months compared with intubation in the hospital.
A Study to Assess the Knowledge Regarding Buteyko Breathing Technique among A...ijtsrd
Asthma is an incurable disease of inflammation of the airways. In affected patients, inflammation causes recurrent episodes of shortness of breath, shortness of breath, chest tightness and coughing, especially at night or very early in the morning. The objectives of the study were to test information on Buteykos respiratory system. The Quantitative test method was adopted in the current study. 60 patients with Bronchial asthma who experienced an inclusive procedure were selected for a simplified procedure. The study was conducted at Medanta Hospital, Lucknow. Data were collected using information questionnaires to assess information on breathing exercises among OPD asthmatic patients. These study results show that out of 60 samples 10 16.6 had sufficient information and 16 26.7 had limited information and 34 56.7 had sufficient information. The study found that the majority of respondents who participated in the study had insufficient knowledge about breathing exercises very few participants are experienced enough. Miss. Gunjan Singh | Mr. Raghavendran M. "A Study to Assess the Knowledge Regarding Buteyko Breathing Technique among Asthma Patients" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd47629.pdf Paper URL : https://www.ijtsrd.com/medicine/nursing/47629/a-study-to-assess-the-knowledge-regarding-buteyko-breathing-technique-among-asthma-patients/miss-gunjan-singh
The In Vivo Effects of Asplenum Bulbiferum Ethanolic Leaf Extracts on Four Co...ijtsrd
Lung diseases have been a worldwide health problem, with high endemicity in developing countries. Bronchopneumonia remains the main disease of the lung. Despite advances in modern medicines, there is no effective drug available that stimulate lung function, offer protection to the lung from damage or help to regenerate pulmonary cells destroyed by disseminated bacteria. There is an urgent need for effective drugs to replace supplement those in current use. This study was embacked upon to evaluate the effect of Asplenium bulbiferum leaf extracts on nosily infected mice with streptococcus pneumoniae isolate of suspension containing 5x106 cells ul. Streptococcus pneumoniae was obtained from human specimen, identified through cultural, morphological and biochemical examination, in addition, polymerase chain reaction PCR technique was performed using universal primers to support the identification process. The antibacterial activity was carried out using agar well diffusion technique. Tube dilution technique was used to determine the minimum Inhibition Concentration MIC and Minimum Bactericidal Concentration MBC using double fold serial dilution at concentrations of 400mg ml to 25mg ml. The effect of the leaf extract was evaluated by treating mus musculus mice WBC count 4.5x103 with sub MIC of A. bulbiferum extracts after nosily infected with S. pneumoniae and appearance of disease septum with 11.3x103 WBC count after 5days from infection. The photochemical analysis revealed the presence of alkaloids phenolics, tannins, saponins, flavonoids, steroids and glycosides. The ethanolic extract exhibited more activity than the aqueous extract against S. aureus most followed by S. pneumoniae, H. influenza and K. pneumoniae was the least. After treating the infected mice with the plant extract and after 6 days from infection, the WBC count reduced to 5.1x103 l which is normal range and the mice were healthy with good physiological behaviour. The infected mice and untreated with extracts, the WBC and other immunological parameters remain high even after 16 days from infection. The study showed that Asplenium bulbiferum possessed antibacterial properties and should serve as alternative therapy for ameliorating lung infections. Eze, H. C | Ajogwu, T. M | Ozor C. N | Gloria, C. I | Soludo, O. C "The In-Vivo Effects of Asplenum Bulbiferum Ethanolic Leaf Extracts on Four Common Bacteria Associated with Lung Diseases" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd47622.pdf Paper URL : https://www.ijtsrd.com/biological-science/microbiology/47622/the-invivo-effects-of-asplenum-bulbiferum-ethanolic-leaf-extracts-on-four-common-bacteria-associated-with-lung-diseases/eze-h-c
Evaluation of the safety of conventional lighting replacement by artificial d...Prof. Hesham N. Mustafa
Background
Short morning exposure to high illuminance visible electromagnetic radiations termed as artificial daylight is beneficial for the mental health of people living in geographical areas with important seasonal changes in daylight illuminance. However, the commercial success of high illuminance light sources has raised the question of the safety of long hour exposure.
Methods
We have investigated the effect of the replacement of natural daylight by artificial daylight in Swiss mice raised under natural lighting conditions. Mice were monitored for neurotoxicity and general health changes. They were submitted to a battery of conventional tests for mood, motor and cognitive functions’ assessment on exposure day (ED) 14 and ED20. Following sacrifice on ED21 due to marked signs of neurotoxicity, the expression of markers of inflammation and apoptosis was assessed in the entorhinal cortex and neurons were estimated in the hippocampal formation.
Results
Signs of severe cognitive and motor impairments, mood disorders, and hepatotoxicity were observed in animals exposed to artificial daylight on ED20, unlike on ED14 and unlike groups exposed to natural daylight or conventional lighting. Activated microglia and astrocytes were observed in the entorhinal cortex, as well as dead and dying neurons. Neuronal counts revealed massive neuronal loss in the hippocampal formation.
Conclusions
These results suggest that long hour exposure to high illuminance visible electromagnetic radiations induced severe alterations in brain function and general health in mice partly mediated by damages to the neocortex-entorhinal cortex-hippocampus axis. These findings raise caution over long hour use of high illuminance artificial light.
Acupressure, reflexology, and auricular acupressure for insomnia: a systemati...RONSA1
Previous randomized controlled trials (RCTs) have shown that acupuncture may be efficacious for insomnia. Instead of needling, acupressure, reflexology, and auricular acupressure are procedures involving physical pressure on acupoints or reflex areas. These variants of acupuncture are gaining popularity, perhaps due to their non-invasive nature. A systematic review has therefore been conducted to examine their efficacy and safety for insomnia. Two independent researchers searched five English and 10 Chinese databases from inception to May 2010. Forty RCTs were identified for analysis. Only 10 studies used sham controls, four used double-blind design, nine studies scored three or more by the Jadad scale, and all had at least one domain with high risk of bias. Meta-analyses of the moderate-quality RCTs found that acupressure as monotherapy fared marginally better than sham control. Studies that compared auricular acupressure and sham control showed equivocal results. It was also found that acupressure, reflexology, or auricular acupressure as monotherapy or combined with routine care was significantly more efficacious than routine care or no treatment. Owing to the methodological limitations of the studies and equivocal results, the current evidence does not allow a clear conclusion on the benefits of acupressure, reflexology, and auricular acupressure for insomnia.
Prehospital rapid sequence intubation improves functional outcome for patient...Emergency Live
In adults with severe TBI, prehospital rapid sequence intubation by paramedics increases the rate of favorable neurologic outcome at 6 months compared with intubation in the hospital.
A Study to Assess the Knowledge Regarding Buteyko Breathing Technique among A...ijtsrd
Asthma is an incurable disease of inflammation of the airways. In affected patients, inflammation causes recurrent episodes of shortness of breath, shortness of breath, chest tightness and coughing, especially at night or very early in the morning. The objectives of the study were to test information on Buteykos respiratory system. The Quantitative test method was adopted in the current study. 60 patients with Bronchial asthma who experienced an inclusive procedure were selected for a simplified procedure. The study was conducted at Medanta Hospital, Lucknow. Data were collected using information questionnaires to assess information on breathing exercises among OPD asthmatic patients. These study results show that out of 60 samples 10 16.6 had sufficient information and 16 26.7 had limited information and 34 56.7 had sufficient information. The study found that the majority of respondents who participated in the study had insufficient knowledge about breathing exercises very few participants are experienced enough. Miss. Gunjan Singh | Mr. Raghavendran M. "A Study to Assess the Knowledge Regarding Buteyko Breathing Technique among Asthma Patients" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd47629.pdf Paper URL : https://www.ijtsrd.com/medicine/nursing/47629/a-study-to-assess-the-knowledge-regarding-buteyko-breathing-technique-among-asthma-patients/miss-gunjan-singh
The In Vivo Effects of Asplenum Bulbiferum Ethanolic Leaf Extracts on Four Co...ijtsrd
Lung diseases have been a worldwide health problem, with high endemicity in developing countries. Bronchopneumonia remains the main disease of the lung. Despite advances in modern medicines, there is no effective drug available that stimulate lung function, offer protection to the lung from damage or help to regenerate pulmonary cells destroyed by disseminated bacteria. There is an urgent need for effective drugs to replace supplement those in current use. This study was embacked upon to evaluate the effect of Asplenium bulbiferum leaf extracts on nosily infected mice with streptococcus pneumoniae isolate of suspension containing 5x106 cells ul. Streptococcus pneumoniae was obtained from human specimen, identified through cultural, morphological and biochemical examination, in addition, polymerase chain reaction PCR technique was performed using universal primers to support the identification process. The antibacterial activity was carried out using agar well diffusion technique. Tube dilution technique was used to determine the minimum Inhibition Concentration MIC and Minimum Bactericidal Concentration MBC using double fold serial dilution at concentrations of 400mg ml to 25mg ml. The effect of the leaf extract was evaluated by treating mus musculus mice WBC count 4.5x103 with sub MIC of A. bulbiferum extracts after nosily infected with S. pneumoniae and appearance of disease septum with 11.3x103 WBC count after 5days from infection. The photochemical analysis revealed the presence of alkaloids phenolics, tannins, saponins, flavonoids, steroids and glycosides. The ethanolic extract exhibited more activity than the aqueous extract against S. aureus most followed by S. pneumoniae, H. influenza and K. pneumoniae was the least. After treating the infected mice with the plant extract and after 6 days from infection, the WBC count reduced to 5.1x103 l which is normal range and the mice were healthy with good physiological behaviour. The infected mice and untreated with extracts, the WBC and other immunological parameters remain high even after 16 days from infection. The study showed that Asplenium bulbiferum possessed antibacterial properties and should serve as alternative therapy for ameliorating lung infections. Eze, H. C | Ajogwu, T. M | Ozor C. N | Gloria, C. I | Soludo, O. C "The In-Vivo Effects of Asplenum Bulbiferum Ethanolic Leaf Extracts on Four Common Bacteria Associated with Lung Diseases" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-6 , October 2021, URL: https://www.ijtsrd.com/papers/ijtsrd47622.pdf Paper URL : https://www.ijtsrd.com/biological-science/microbiology/47622/the-invivo-effects-of-asplenum-bulbiferum-ethanolic-leaf-extracts-on-four-common-bacteria-associated-with-lung-diseases/eze-h-c
Evaluation of the safety of conventional lighting replacement by artificial d...Prof. Hesham N. Mustafa
Background
Short morning exposure to high illuminance visible electromagnetic radiations termed as artificial daylight is beneficial for the mental health of people living in geographical areas with important seasonal changes in daylight illuminance. However, the commercial success of high illuminance light sources has raised the question of the safety of long hour exposure.
Methods
We have investigated the effect of the replacement of natural daylight by artificial daylight in Swiss mice raised under natural lighting conditions. Mice were monitored for neurotoxicity and general health changes. They were submitted to a battery of conventional tests for mood, motor and cognitive functions’ assessment on exposure day (ED) 14 and ED20. Following sacrifice on ED21 due to marked signs of neurotoxicity, the expression of markers of inflammation and apoptosis was assessed in the entorhinal cortex and neurons were estimated in the hippocampal formation.
Results
Signs of severe cognitive and motor impairments, mood disorders, and hepatotoxicity were observed in animals exposed to artificial daylight on ED20, unlike on ED14 and unlike groups exposed to natural daylight or conventional lighting. Activated microglia and astrocytes were observed in the entorhinal cortex, as well as dead and dying neurons. Neuronal counts revealed massive neuronal loss in the hippocampal formation.
Conclusions
These results suggest that long hour exposure to high illuminance visible electromagnetic radiations induced severe alterations in brain function and general health in mice partly mediated by damages to the neocortex-entorhinal cortex-hippocampus axis. These findings raise caution over long hour use of high illuminance artificial light.
Secondary brain injury is a frequent event in TBI patients.
These events greatly influence prognosis and are potentially preventable.
Our understanding of secondary brain injury mechanisms and physiologic responses to treatment is evolving.
Session Title:
Radionuclide Therapy Basics, General Regulations and Update on I-131 Rx
Presented at the Annual Meeting of the Society of Nuclear Medicine and Molecular Imaging in Denver, CO on Sunday, June 11, 3:00PM–4:30PM
Management of Neurological Manifestations in Covid 19 through AyurvedaYogeshIJTSRD
Ayurveda is a system of traditional Indian medicine which is based on sound therapeutic principles and has a proven history of empirical use. Hence, Dhatuposhana and administration of Rasayana drugs like Draksha Vitis vinifera L. , Amalaki, Gudhuchi, Vasa Justicia adhatoda L. for at least 45 days and to combat the residual effects of the virus on the body – Vishaghna chikitsa with Shirisha Albizia lebbeck L. Benth. recovery. Deepana Pachana drugs like Shadanga Paneeya may be used in case of Diarrhoea, vomiting or loss of appetite. Depending on the condition of Dhatu and Dosha, Shamana in the form of Brimhana chikitsa can be adopted to restore the function of Tridosha beside this Satvavajaya Chikitsa ayurvedic or Haridra churna Curcuma longa is suggested after clinical psychotherapy Sadvritta, and Achara Rasayana behavioural therapy can play an important role to manage post covid symptoms these are the non pharmacological approach aimed to maintain mental health. Regular practice of yogasana and pranayama with achara rasayana is useful for mental stability. Dr. (Vaidya) Ravi Sharma "Management of Neurological Manifestations in Covid-19 through Ayurveda" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd43778.pdf Paper URL: https://www.ijtsrd.com/medicine/ayurvedic/43778/management-of-neurological-manifestations-in-covid19-through-ayurveda/dr-vaidya-ravi-sharma
Intensive Aphasia Treatment Program
The North Memorial Stroke Center is the first in the region to offer this innovative outpatient program that includes many hours of intense therapy over the course of three weeks. The program is designed to help participants relearn old ways or develop new alternative ways of communicating. Patients participate in a combination of individual and group treatments sessions for approximately three and a half hours per day, five days a week.
Prevalence of nerve conduction study to determine the prognostic value in Bel...AI Publications
Objective: To assess and compare side to side nerve conduction amplitude and degree of recovery facial nerve in bell’s palsy patients. METHODS: The present study was conducted on 23 Bell’s palsy patients (Aged 20-40 years) within 2week after onset of disease, in department of physiology with collaboration with department of ENT in JLN medical college and attached hospitals, Ajmer. Motor nerve conduction and latency recorded. Patient was made to lie down and surface electrodes were fixed over the skin which is on the nerve and supplying muscle. By stimulating the nerve at two different point and record motor evoke response. Result: Motor response is reduced in facial nerve; If motor response is <10% of normal (moderate-to-severe).recovery time is 6-12month.; If motor response is 10-30% of normal (mild-to-moderate) recovery time is 2-8month.; If motor response is >30% of normal complete recovery expected within 2month. Conclusion: Present study concluded that early recovery (within 2 months) recorded in patients with motor response is >30% of normal as compared to patients with motor response is <10% compared to healthy side. (Within 6-12 months). NCS is used to as an important tool to diagnose and prognoses the facial neuropathy in the Bell’s palsy patients. Significance: Present Study signifies the importance of nerve conduction studies in early initiation of treatment and recovery of facial neuropathy in Bell’s palsy patients.
Effect and maintenance of "EEG-biofeedback rTMS" on mood and working memory ...Amin Asadollahpour Kargar
This is a proposal presented in the 1st IBRO/APRC Iranian Associate School of Cognitive Neuroscience “Functional Human Brain Mapping”, Tehran, Iran, May 22-28, 2015
aimed:
1. To evaluate the effect of EEG-biofeedback rTMS on Mood in major depressed patients compare to EEG biofeedback and rTMS
2. To evaluate the maintenance of EEG-biofeedback rTMS on working memory in major depressed patients compare to EEG biofeedback and rTMS
Secondary brain injury is a frequent event in TBI patients.
These events greatly influence prognosis and are potentially preventable.
Our understanding of secondary brain injury mechanisms and physiologic responses to treatment is evolving.
Session Title:
Radionuclide Therapy Basics, General Regulations and Update on I-131 Rx
Presented at the Annual Meeting of the Society of Nuclear Medicine and Molecular Imaging in Denver, CO on Sunday, June 11, 3:00PM–4:30PM
Management of Neurological Manifestations in Covid 19 through AyurvedaYogeshIJTSRD
Ayurveda is a system of traditional Indian medicine which is based on sound therapeutic principles and has a proven history of empirical use. Hence, Dhatuposhana and administration of Rasayana drugs like Draksha Vitis vinifera L. , Amalaki, Gudhuchi, Vasa Justicia adhatoda L. for at least 45 days and to combat the residual effects of the virus on the body – Vishaghna chikitsa with Shirisha Albizia lebbeck L. Benth. recovery. Deepana Pachana drugs like Shadanga Paneeya may be used in case of Diarrhoea, vomiting or loss of appetite. Depending on the condition of Dhatu and Dosha, Shamana in the form of Brimhana chikitsa can be adopted to restore the function of Tridosha beside this Satvavajaya Chikitsa ayurvedic or Haridra churna Curcuma longa is suggested after clinical psychotherapy Sadvritta, and Achara Rasayana behavioural therapy can play an important role to manage post covid symptoms these are the non pharmacological approach aimed to maintain mental health. Regular practice of yogasana and pranayama with achara rasayana is useful for mental stability. Dr. (Vaidya) Ravi Sharma "Management of Neurological Manifestations in Covid-19 through Ayurveda" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd43778.pdf Paper URL: https://www.ijtsrd.com/medicine/ayurvedic/43778/management-of-neurological-manifestations-in-covid19-through-ayurveda/dr-vaidya-ravi-sharma
Intensive Aphasia Treatment Program
The North Memorial Stroke Center is the first in the region to offer this innovative outpatient program that includes many hours of intense therapy over the course of three weeks. The program is designed to help participants relearn old ways or develop new alternative ways of communicating. Patients participate in a combination of individual and group treatments sessions for approximately three and a half hours per day, five days a week.
Prevalence of nerve conduction study to determine the prognostic value in Bel...AI Publications
Objective: To assess and compare side to side nerve conduction amplitude and degree of recovery facial nerve in bell’s palsy patients. METHODS: The present study was conducted on 23 Bell’s palsy patients (Aged 20-40 years) within 2week after onset of disease, in department of physiology with collaboration with department of ENT in JLN medical college and attached hospitals, Ajmer. Motor nerve conduction and latency recorded. Patient was made to lie down and surface electrodes were fixed over the skin which is on the nerve and supplying muscle. By stimulating the nerve at two different point and record motor evoke response. Result: Motor response is reduced in facial nerve; If motor response is <10% of normal (moderate-to-severe).recovery time is 6-12month.; If motor response is 10-30% of normal (mild-to-moderate) recovery time is 2-8month.; If motor response is >30% of normal complete recovery expected within 2month. Conclusion: Present study concluded that early recovery (within 2 months) recorded in patients with motor response is >30% of normal as compared to patients with motor response is <10% compared to healthy side. (Within 6-12 months). NCS is used to as an important tool to diagnose and prognoses the facial neuropathy in the Bell’s palsy patients. Significance: Present Study signifies the importance of nerve conduction studies in early initiation of treatment and recovery of facial neuropathy in Bell’s palsy patients.
Effect and maintenance of "EEG-biofeedback rTMS" on mood and working memory ...Amin Asadollahpour Kargar
This is a proposal presented in the 1st IBRO/APRC Iranian Associate School of Cognitive Neuroscience “Functional Human Brain Mapping”, Tehran, Iran, May 22-28, 2015
aimed:
1. To evaluate the effect of EEG-biofeedback rTMS on Mood in major depressed patients compare to EEG biofeedback and rTMS
2. To evaluate the maintenance of EEG-biofeedback rTMS on working memory in major depressed patients compare to EEG biofeedback and rTMS
Homoeopathic Drug Standardization through Biological Evaluation Future Perspe...ijtsrd
There is a scarcity of chemico analytical method of standardization of high dilution of homoeopathic drugs. Homoeopathic medicines include any drug which are prepared by the methods given in homoeopathic pharmacopoeias. Efficacy of homoeopathic medicines are obtained by clinical use, from homoeopathic authenticated literature and research. Vineeta | Atul Kumar Singh "Homoeopathic Drug Standardization through Biological Evaluation- Future Perspective" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-6 , October 2020, URL: https://www.ijtsrd.com/papers/ijtsrd33596.pdf Paper Url: https://www.ijtsrd.com/medicine/other/33596/homoeopathic-drug-standardization-through-biological-evaluation-future-perspective/vineeta
1. 1
Curriculum Vitae -- Yu-Wen Li
Genetically-Defined Diseases, Research & Development, Bristol-Myers Squibb Company
3CD-442, 5 Research Parkway, Wallingford, CT, 06492-1996
Tel: 203-677 7458 Fax: 203-677 7569 Email: yuwenli@yahoo.com
Summary
Scientific team leader with 20 year experience in pharmaceutical drug discovery research.
Successfully provide critical support for multiple early and full phase programs include small
molecule, antisense oligonucleotide and gene therapies for psychiatric diseases (depression,
Schizophrenia, Rett syndrome), neurodegenerative diseases, neuropathic pain and heart failure
programs. Highly skilled in leading research teams to use a variety of in vitro, ex vivo and in vivo
approaches for target validation, understanding of mechanisms of action and characterization of
PK-PD relationships of drugs. Authorized over 40 papers with 12 as the first or senior author
since joining the pharmaceutical industry.
Pharmaceutical ResearchExperience
Senior Principal Scientist 2014 – present
Genetically-Defined Diseases, Bristol-Myers Squibb
- Managed a core group with 4 research scientists
o Biochemistry lab
generated critical data to understand biodistribution (PK-PD) of target
proteins (transgenes) in cardiac and skeletal muscles following systemic
delivery of AAV-mediated gene using fluorescence
immunohistochemistry (IHC) and confocal imaging acquisition and
analysis.
developed and utilized fluorescence in-situ hybridization (FISH) assays to
investigate X-chromosome reactivation in primary neuronal cultures,
embryonic fibroblastic cells in response to treatment with antisense
oligonucleotides (ASOs) and small molecules, and in target KO mouse
brain tissues as part of target validation.
characterized ASO uptake and target knockdown effects of ASOs in
disease relevant brain regions of rodents and cynomolgus monkeys using
mRNA in situ hybridization (ISH) assays to support multiple ASO
programs.
established and used an organotypic cerebellar slice culture assay to
characterize leading Immunoscience program compounds for treatment of
multiple sclerosis.
o In vivo electrophysiology lab
established and used in vivo spinal electrophysiology assays to evaluate
effects of novel analgesic agents on windup and hyperactivity of spinal
dorsal horn in chronic constriction injury model of neuropathic pain.
2. 2
established and used in vivo DRG neuronal recording assays to evaluate
effects of peripheral acting analgesic agents on DRG neuronal activity
- Contributed to 2 study reports for IND preparations.
- Authorized 10 publications including 4 as the first/senior author.
Senior Principal Scientist
Neuroscience Biology, Bristol-Myers Squibb 2007 – 2013
- Managed a core group with 6 research scientists (including one PhD)
o Biochemistry lab
used in vitro and ex vivo binding/occupancy assays (autoradiography and
homogenate binding) to support multiple early and full-phase psychiatry
and neuropathic pain programs.
used a number of ex vivo biochemistry assays using IHC & ISH
approaches to support early and full-phase discovery programs in
psychiatry, neuropathic pain and Tau neuropathy.
established a unique focused microwave irradiation-assisted IHC
assay to study phosphor-protein immunoreactivity in response to
drug treatment.
o Electrophysiology lab
established a unique ex vivo LTP assay as a surrogate readout of synaptic
plasticity to study long lasting effects of novel antidepressant drugs.
- Led to establish a biochemistry lab in the Biocon Bristol-Myers Squibb Research &
Development Center in India, and provided supervision and guidance on regular basis.
- Contributed to 5 study reports for IND preparations.
- Authorized 15 publications including 4 as the first/senior author
Principal Scientist 2004 - 2006
Neuroscience Biology, Bristol-Myers Squibb
- Managed a core group with 4 research scientists
- Provided ex vivo binding occupancy assay support for 5 full-phase programs
- Played a leading role in PET ligand development/PET imaging studies for neuroscience
programs
- Pioneered ex vivo homogenate occupancy assay automation and efficiency improvement
project in collaboration with Applied Biotechnology Discovery Biology Automation group
- Chaired one early-phase drug discovery program
- Contributed to 4 study reports for IND preparations.
- Authorized 4 publications
Senior Research Investigator II 2002 - 2004
Neuroscience Biology, Bristol-Myers Squibb
- Managed one core group with 4 research scientists
- Supported two full-phase and two early phase drug discovery programs in neurological
and psychiatric diseases
- Used ex vivo and in vivo ligand binding and imaging analysis assays for evaluation of
brain penetrance and site occupancy of test compounds
- Established an 18F in vitro biology lab to support PET ligand development
3. 3
- Contributed to 2 study reports for IND preparations
- Authorized 7 publications
Research/Senior ResearchScientist 1999 - 2001
CNS Diseases Research, DuPont Pharmaceuticals
- Managed one core function lab with 4 research scientists
- Supported two full-phase and one early phase drug discovery programs in neurological
and psychiatric, metabolic (obesity) diseases
- Developed competitive in vitro, ex vivo and in vivo ligand binding in brain section assays
and imaging analysis procedures for evaluating brain penetrance and site occupancy of
program compounds
- Developed in vivo electrophysiology assays to support analgesic drug discovery programs
ResearchScientist 1997 - 1998
Computational Biology Group, Central Research& Development, DuPont Company
- Developed in vitro and in vivo electrophysiology models to investigate neuronal
integration and modulation
- Collaborated internally and externally to investigate central regulation of
cardiorespiratory functions.
Academic ResearchExperience
Post-Doctoral ResearchFellow, American Heart Association 1993 - 1996
Department of Pharmacology, University of Virginia
- Investigated central mechanisms responsible regulation of cardiovascular functions using
whole-cell patch clamp in brain slide preparations
Post-Doctoral ResearchFellow, NH&MRC of Australia 1991 - 1992
Department of Physiology University of Sydney, Australia
Ph.D Candidate 1988 - 1990
Department of Medicine and Physiology, Flinders University of South Australia
- Developed and/or applied a variety of electrophysiological and anatomical techniques
including single and multiple unit recording, central microinjection, trans-synaptic
tracing with virus and c-fos functional mapping to investigate central pathways
responsible for control and regulation of cardiovascular functions
ResearchFellow, Australian-China Council 1986 - 1987
Department of Physiology Flinders University of South Australia
- Studied topographic and morphologic changes of brain monoaminergic and peptidergic
neurons in Parkinson's patients using immunohistochemistry and computer-image
analysis techniques
Assistant Professor 1985 - 1986
MasterDegree of Medical Science Candidate 1983 - 1985
Department of Human Anatomy, Hunan Medical University, China
4. 4
- Investigated prenatal development of human brain catecholaminergic neurons using
histofluorescence techniques
Intern in Internal Medicine 1982 - 1983
Anhui Medical College Hospital, China
EDUCATION
Ph.D in Neuroscience and Physiology 1991
Flinders University of South Australia, Australia
Advisor: W.W. Blessing, PhD, MD, Senior Principal Research Fellow of NH&MRC
Thesis: Central mechanisms of cardiovascular regulation
Masterof Medical Science in Neurobiology 1985
Hunan Medical University, Changsha, China
Advisor: H.S. Su, MD
Thesis: Monoaminergic neurons: Distribution in rodent and human brains
Bachelor of Medicine 1982
Anhui Medical College, China
5. 5
PUBLICATION LIST
1. Su, H.S., Peng, Z.C. and Li, Y.-W. (1987) Distribution of catecholamine-containing cell bodies in the human
diencephalon. Brain Research 409, 367-370.
2. Li, Y.-W., Halliday, G.M., Joh, T.H., Geffen, L.B. and Blessing, W.W. (1988) Tyrosine hydroxylase-
containing neurons in the supraoptic and paraventricular nuclei of the adult human. Brain Research 461, 75-
86.
3. Halliday, G.M., Li, Y.-W., Oliver, J.R., Joh, T.H., Cotton, R.G.H., Howe, P.R.C., Geffen, L.B. and Blessing,
W.W. (1988) The distribution of neuropeptide Y-like immunoreactive neurons in the human medulla
oblongata. Neuroscience 26, 179-191.
4. Halliday, G.M., Li, Y.-W., Hoh, T.H., Cotton, R.G.H., Howe, P.R.C., Geffen, L.B. and Blessing, W.W.
(1988) Monoamine-synthesizing neurons in the human medulla oblongata. Journal of Comparative
Neurology 273, 301-317.
5. Halliday, G.M., Li, Y.-W., Joh, T.H., Cotton, R.G.H., Howe, P.R.C., Geffen, L.B. and Blessing, W.W.
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