Presentation of Richard Murphy for the Workshop on Hydrolysis Route for Cellulosic Ethanol from Sugarcane.
Apresentação de Richard Murphy realizada no "Workshop on Hydrolysis Route for Cellulosic Ethanol from Sugarcane"
Date / Data : February 10 - 11th 2009/
10 e 11 de fevereiro de 2009
Place / Local: Unicamp, Campinas, Brazil
Event Website / Website do evento: http://www.bioetanol.org.br/workshop1
Detection of Genetically Modified Soybean in Crude Soybean Oil.PDFGordana Zdjelar
This document describes a study that aimed to detect and quantify the presence of Roundup Ready genetically modified soybean in crude soybean oil extracted from soybean seed with varying percentages of GM content. Two DNA extraction methods were compared: CTAB and a commercial DNeasy Plant Mini Kit. Real-time PCR was used to amplify DNA targets from the extracted samples to detect and quantify the GM content. The results showed that the commercial kit was superior to CTAB extraction for recovering DNA from crude oil samples. Both qualitative and quantitative PCR analysis demonstrated it is possible to monitor the GM content in crude oil derived from soybean seed with varying GM percentages.
Screening cassava germplasm for ß-carotene,Evaluation of ß-carotene-rich genotypes: agronomic
characteristics,Introduction of germplasm from within and outside SSA,Gene introgression for the improvement of ß-carotene levels..
Old man saltbush is a key species for grazing systems in dry areas of Australia. Researchers screened over 80,000 saltbush plants from 27 locations to develop superior cultivars. They identified significant variation in traits like biomass and palatability. Based on multi-year trials of over 20,000 plants, they selected elite clones and are developing seed cultivars to improve establishment and reduce costs for farmers. The new cultivars show promise to boost the resilience and productivity of grazing lands across southern Australia.
Stephen Friend Fanconi Anemia Research Fund 2012-01-21Sage Base
This document summarizes Stephen Friend's presentation on using data intensive science and bionetworks to build better maps of human diseases. It discusses how collecting and integrating massive amounts of molecular and clinical data using open information systems and computing could enable the development of more comprehensive and probabilistic causal models of diseases. These evolving disease maps may help identify causal genes and pathways involved in various conditions. The presentation outlines Sage Bionetworks' mission to create a commons for scientists to collaborate on building and refining such integrative bionetworks to accelerate the elimination of human disease.
This document summarizes Michael Buschmann's work on nanomedicine at Ecole Polytechnique. It discusses how nanomedicine uses nano-sized tools for diagnosis, prevention and treatment of disease. Some key applications of nanomedicine include drug delivery via liposomes and polymeric nanoparticles. The document also outlines the requirements for successful nanomedicine research and development, including efficacy, safety, manufacturing and regulatory approval. Buschmann's group works on developing chitosan-based nanoparticles for gene delivery applications.
Rice breeding is both challenged and benefited by the fact that a successful varietal improvement program must embrace both the integration single genes that segregate in a simple Mendelian fashion as well as complex traits that are inherited in more quantitative ways. For decades the rice genetics community has produced a wealth of knowledge about these single genes and has developed markers that allow a breeder to track them in a population. However, marker assisted selection (MAS) alone is insufficient to drive the rates of genetic gain for more complex traits that are equally necessary. This presentation will describe the attempts made in the Favorable Environments Breeding program at IRRI to integrate the selection for single genes appropriate for MAS into a more complex population improvement strategy designed to improve quantitatively inherited traits.
Detection of Genetically Modified Soybean in Crude Soybean Oil.PDFGordana Zdjelar
This document describes a study that aimed to detect and quantify the presence of Roundup Ready genetically modified soybean in crude soybean oil extracted from soybean seed with varying percentages of GM content. Two DNA extraction methods were compared: CTAB and a commercial DNeasy Plant Mini Kit. Real-time PCR was used to amplify DNA targets from the extracted samples to detect and quantify the GM content. The results showed that the commercial kit was superior to CTAB extraction for recovering DNA from crude oil samples. Both qualitative and quantitative PCR analysis demonstrated it is possible to monitor the GM content in crude oil derived from soybean seed with varying GM percentages.
Screening cassava germplasm for ß-carotene,Evaluation of ß-carotene-rich genotypes: agronomic
characteristics,Introduction of germplasm from within and outside SSA,Gene introgression for the improvement of ß-carotene levels..
Old man saltbush is a key species for grazing systems in dry areas of Australia. Researchers screened over 80,000 saltbush plants from 27 locations to develop superior cultivars. They identified significant variation in traits like biomass and palatability. Based on multi-year trials of over 20,000 plants, they selected elite clones and are developing seed cultivars to improve establishment and reduce costs for farmers. The new cultivars show promise to boost the resilience and productivity of grazing lands across southern Australia.
Stephen Friend Fanconi Anemia Research Fund 2012-01-21Sage Base
This document summarizes Stephen Friend's presentation on using data intensive science and bionetworks to build better maps of human diseases. It discusses how collecting and integrating massive amounts of molecular and clinical data using open information systems and computing could enable the development of more comprehensive and probabilistic causal models of diseases. These evolving disease maps may help identify causal genes and pathways involved in various conditions. The presentation outlines Sage Bionetworks' mission to create a commons for scientists to collaborate on building and refining such integrative bionetworks to accelerate the elimination of human disease.
This document summarizes Michael Buschmann's work on nanomedicine at Ecole Polytechnique. It discusses how nanomedicine uses nano-sized tools for diagnosis, prevention and treatment of disease. Some key applications of nanomedicine include drug delivery via liposomes and polymeric nanoparticles. The document also outlines the requirements for successful nanomedicine research and development, including efficacy, safety, manufacturing and regulatory approval. Buschmann's group works on developing chitosan-based nanoparticles for gene delivery applications.
Rice breeding is both challenged and benefited by the fact that a successful varietal improvement program must embrace both the integration single genes that segregate in a simple Mendelian fashion as well as complex traits that are inherited in more quantitative ways. For decades the rice genetics community has produced a wealth of knowledge about these single genes and has developed markers that allow a breeder to track them in a population. However, marker assisted selection (MAS) alone is insufficient to drive the rates of genetic gain for more complex traits that are equally necessary. This presentation will describe the attempts made in the Favorable Environments Breeding program at IRRI to integrate the selection for single genes appropriate for MAS into a more complex population improvement strategy designed to improve quantitatively inherited traits.
“Fuelling Ontario’s Greenhouse Industry with Biomass”, a presentation by Dean Tiessen, Pyramid Farms Ltd., at the Growing the Margins Conference held April 2-5, 2008
Andy Robertson - Miscanthus trace gases - CEH Presentation March 2013Andy Robertson
This talk was given to fellow PhD candidates at the Centre for Ecology and Hydrology in Lancaster in March 2013. Other attendees were professors and senior researchers within CEH. The presentation describes the experiment and preliminary results from a study of trace gas emissions from a Miscanthus plantation in the UK.
Energy Crops Pellets: a Revolution for MiscanthusJossie Xiong
iscanthus is a kind of perennial energy crop that grows over three meters tall, resembles bamboo and produces the crop every year without replanting. The rapid growth, low mineral content and high yield have made miscanthus emerging as biomass fuel.
The document contains 7 figures and 1 table describing the characteristics and cultivation of Giant Miscanthus, a perennial grass used for bioenergy. Figure 1 shows Giant Miscanthus being grown on a farm in Illinois. Figure 2 depicts the suitable growing range for Giant Miscanthus, which prefers areas with at least 30 inches of annual rainfall. Figure 3 compares the annual biomass yields of Giant Miscanthus and switchgrass in Illinois.
Earth Sense Energy Usa Ppt 09 28 09 2003 Versionguest945bcc
Earth Sense Energy USA was created to capitalize on opportunities in the emerging biomass economy. The company plans to be the largest producer of the energy grass Miscanthus in the US within 3-5 years by producing and selling Miscanthus transplants and providing establishment services to farmers. This will help achieve projections of millions of acres of biomass production needed for fuel. Earth Sense Energy USA aims to vertically integrate into related markets like carbon trading and biomass conversion to maximize opportunities from the growing biomass sector.
Energy crops grown in Poland include shrubs like shrubby willow and poplar, perennial plants like Virginia Fan Petals and Jerusalem artichoke, and perennial grasses like miscanthus, prairie cordgrass, big bluestem, and switchgrass. Shrubby willow is well-suited for energy purposes with high yields over 15 years but has disadvantages like high moisture content, difficult mechanized harvesting, and large water needs. White poplar has lower soil and water requirements than willow and is suitable for machine harvesting but requires weed control. Virginia Fan Petals can be harvested with agricultural machinery, grows on weaker soils, and has low ash and mineral content but has poor seed
The document describes a study that used the DAYCENT model with site-specific environmental data to simulate sorghum biomass yield, soil organic carbon, and nitrous oxide emissions across cultivated lands in the continental United States. The results suggest over 10 million hectares in the Southern and Lower Midwestern US could produce over 10 megagrams per hectare per year of biomass sorghum with net carbon sequestration under rainfed conditions. The methodology developed provides an opportunity to explore other bioenergy crops, and the high resolution spatially explicit results are critical inputs for life-cycle assessments and climate change mitigation strategies.
This document summarizes a study that developed an automated sample preparation workflow for high-throughput quantitative proteomic analysis of microbes. Key points:
- An integrated automation platform was developed to support automated sample preparation from cell pellets to tryptic digestion for 96 samples in 2 hours or 384 samples simultaneously.
- The automated workflow was tested on two bacteria and two fungi species and achieved high reproducibility while significantly reducing labor compared to manual methods.
- The automated workflow was shown to be an effective method for high-throughput quantitative microbial proteomics through mass spectrometry.
Lentikats Biotechnology é uma tecnologia revolucionária de encapsulamento que confere aos microrganismos (bactérias, leveduras, fungos) e enzimas características únicas necessárias para novas aplicações e inovações em bioprocessos.
Confira detalhes na vitrine de tecnologias da BiorrefinariaTec, uma plataforma de inovação aberta especializada no desenvolvimento das Biorrefinarias.
www.biorrefinariatec.com/vitrine
Microbial Diversity: Tapping the Untappedsachhatre
The document summarizes research on exploring microbial diversity to identify novel microbial functions. It describes enrichment and isolation of hydrocarbon-degrading bacteria from oil sludge to create a bacterial consortium for bioremediating oil spills. Molecular tools were used to characterize the isolates and determine their catabolic pathways. The consortium showed improved degradation of crude oil and model petroleum compounds compared to individual isolates.
This document discusses several papers that apply machine learning and multi-omics data to predict metabolic pathway dynamics.
- One paper develops a machine learning approach using proteomics data instead of kinetic modeling to accurately predict metabolite concentrations. This approach provides faster development of predictive models since it infers knowledge from data rather than requiring domain expertise.
- Another paper applies machine learning and multi-omics data to quantitatively predict production of the biofuel isopentenol from limited training data.
- A third paper reviews how knowledge engineering and data-driven frameworks using machine learning can offer new constraints for mechanistic models to better describe cellular regulation and design metabolic pathways. This facilitates "learn and design" for strain development.
Synthetic Biology for Plant ScientistsSachin Rawat
Tools of synthetic biology can be utilised to engineer metabolic pathways to optimize production of secondary metabolites and ligno-cellulose. The presentation describes an approach to develop an artificial positive feedback loop to increase accumulation of cell wall polysaccharides. These will decrease the cost of production of plant-based biofuels, paper and other plant products.
This document summarizes research on improving Pseudomonas putida KT2440 for the conversion of lignin-derived aromatic compounds into biofuels and bioproducts. Adaptive laboratory evolution was used to evolve P. putida strains with improved tolerance to the hydroxycinnamic acids p-coumaric acid and ferulic acid. Evolved strains showed significant decreases in lag phase and increases in growth rate in high concentrations of these acids. Whole genome sequencing identified genetic mutations conferring these improvements, including in genes like PP_3350 and ttgB. Deletion of these genes in the wild type strain verified their roles in tolerance. This work identifies new targets for engineering P. putida's ability to utilize aromatic compounds
The document discusses membrane bioreactor (MBR) technology for wastewater treatment. MBR combines a biological wastewater treatment process with a membrane filtration process. It provides several advantages over conventional activated sludge including higher quality effluent with very low levels of contaminants, complete pathogen removal, and ability to reuse treated water. The document examines various MBR configurations, design considerations, operating parameters, case studies on MBR use in antibiotic manufacturing wastewater treatment, and concludes that MBR is an effective technology for wastewater treatment applications.
This document discusses bioenergy and various bioenergy crops. It begins by defining bioenergy as renewable energy from biological sources like biomass, agricultural waste, and municipal waste. Key bioenergy crops discussed include sorghum, sugarcane, switchgrass, and miscanthus. The document outlines India's current bioenergy production and promising crops for ethanol and biodiesel. It discusses challenges in breeding perennial bioenergy crops and strategies to improve traits like yield, stress tolerance, and lignin content. Overall, the document presents bioenergy as a renewable and sustainable future fuel that can provide energy security while managing agricultural waste.
The study developed stochastic models to analyze the economic and environmental impacts of biodiesel production from Jatropha curcas in Nepal. The models determined that a minimum seed yield of 3.9 tons/hectare and oil content above 50% by weight are required for the biodiesel selling price to be comparable to conventional diesel prices. Including direct and indirect land use changes, the carbon footprint of Jatropha biodiesel could be lower than conventional fuels if high seed yields above 5 tons/hectare are achieved using only marginal lands and with aggressive afforestation. The results identify pathways for policies to enable sustainable Jatropha biodiesel production.
This document summarizes four research articles from the Office of Biological and Environmental Research.
The first article compares pretreatment processes using two ionic liquids to pretreat aspen and maple biomass for biofuel production. It finds one ionic liquid increased crystallinity while the other reduced it.
The second isolates five new Pseudomonas strains from soil that can metabolize pentose sugars, with genomes sequenced. This expands options for converting biomass into fuels and chemicals.
The third engineers poplar trees to produce less lignin using a bacterial enzyme, making the biomass easier to break down into sugars for biofuels. It reduces lignin by up to 30% while incorporating cleavable bonds into the lignin.
In this world of concerns regarding depletion of fossil fuels, pollution control and other factors leading to threat of man kind survival a way of producing biodiesel from algae which can be a source of alternative fuel. Lots of methods and sources being used for producing biodiesel but from algae one can produce high amount of biodiesel depending on the type of species or strain selected and this way this is a viable and feasible method to produce biodiesel.....
This document summarizes three papers related to engineering plant metabolism and microbial physiology.
The first paper describes engineering the brassinin biosynthetic pathway in plants to produce novel antifungal compounds called crucifalexins. Two of the new compounds showed stronger antifungal activity than brassinin.
The second paper discusses using solid-state NMR to characterize the native architecture of plant cell walls without disruption. This provides information to engineer bioenergy crops.
The third paper combines mechanistic and machine learning models to predictively engineer and optimize tryptophan metabolism in microbes. This approach increased tryptophan titer and productivity by up to 74% and 43% compared to an improved reference strain.
This study analyzed changes in the bacterial and fungal microbiome of soil samples treated with biosolarization, which uses solar heating and organic amendments. Sequencing analysis found that biosolarization had a stronger impact on the relative abundance of bacterial phyla than fungi. Network analysis identified microbial clusters correlated with volatile fatty acid accumulation, suggesting genera like Clostridium, Weissella and Acetobacter can tolerate and potentially produce these compounds. The results provide insight into structural changes in the soil microbiome during biosolarization as related to volatile fatty acid levels.
This study developed genetic tools for the thermophilic fungus Thermoascus aurantiacus, including a transformation system, CRISPR/Cas9 gene editing, and sexual crossing protocol. The transformation system was used to overexpress a transcriptional regulator of xylanase, increasing xylanase activity by up to 500%. CRISPR/Cas9 was shown to successfully delete a target gene with 10-35% efficiency. A sexual crossing protocol allowed crossing and isolation of progeny within a week, enabling strain engineering in this industrially relevant fungus. These tools open new possibilities for engineering thermostable enzyme production in T. aurantiacus.
“Fuelling Ontario’s Greenhouse Industry with Biomass”, a presentation by Dean Tiessen, Pyramid Farms Ltd., at the Growing the Margins Conference held April 2-5, 2008
Andy Robertson - Miscanthus trace gases - CEH Presentation March 2013Andy Robertson
This talk was given to fellow PhD candidates at the Centre for Ecology and Hydrology in Lancaster in March 2013. Other attendees were professors and senior researchers within CEH. The presentation describes the experiment and preliminary results from a study of trace gas emissions from a Miscanthus plantation in the UK.
Energy Crops Pellets: a Revolution for MiscanthusJossie Xiong
iscanthus is a kind of perennial energy crop that grows over three meters tall, resembles bamboo and produces the crop every year without replanting. The rapid growth, low mineral content and high yield have made miscanthus emerging as biomass fuel.
The document contains 7 figures and 1 table describing the characteristics and cultivation of Giant Miscanthus, a perennial grass used for bioenergy. Figure 1 shows Giant Miscanthus being grown on a farm in Illinois. Figure 2 depicts the suitable growing range for Giant Miscanthus, which prefers areas with at least 30 inches of annual rainfall. Figure 3 compares the annual biomass yields of Giant Miscanthus and switchgrass in Illinois.
Earth Sense Energy Usa Ppt 09 28 09 2003 Versionguest945bcc
Earth Sense Energy USA was created to capitalize on opportunities in the emerging biomass economy. The company plans to be the largest producer of the energy grass Miscanthus in the US within 3-5 years by producing and selling Miscanthus transplants and providing establishment services to farmers. This will help achieve projections of millions of acres of biomass production needed for fuel. Earth Sense Energy USA aims to vertically integrate into related markets like carbon trading and biomass conversion to maximize opportunities from the growing biomass sector.
Energy crops grown in Poland include shrubs like shrubby willow and poplar, perennial plants like Virginia Fan Petals and Jerusalem artichoke, and perennial grasses like miscanthus, prairie cordgrass, big bluestem, and switchgrass. Shrubby willow is well-suited for energy purposes with high yields over 15 years but has disadvantages like high moisture content, difficult mechanized harvesting, and large water needs. White poplar has lower soil and water requirements than willow and is suitable for machine harvesting but requires weed control. Virginia Fan Petals can be harvested with agricultural machinery, grows on weaker soils, and has low ash and mineral content but has poor seed
The document describes a study that used the DAYCENT model with site-specific environmental data to simulate sorghum biomass yield, soil organic carbon, and nitrous oxide emissions across cultivated lands in the continental United States. The results suggest over 10 million hectares in the Southern and Lower Midwestern US could produce over 10 megagrams per hectare per year of biomass sorghum with net carbon sequestration under rainfed conditions. The methodology developed provides an opportunity to explore other bioenergy crops, and the high resolution spatially explicit results are critical inputs for life-cycle assessments and climate change mitigation strategies.
This document summarizes a study that developed an automated sample preparation workflow for high-throughput quantitative proteomic analysis of microbes. Key points:
- An integrated automation platform was developed to support automated sample preparation from cell pellets to tryptic digestion for 96 samples in 2 hours or 384 samples simultaneously.
- The automated workflow was tested on two bacteria and two fungi species and achieved high reproducibility while significantly reducing labor compared to manual methods.
- The automated workflow was shown to be an effective method for high-throughput quantitative microbial proteomics through mass spectrometry.
Lentikats Biotechnology é uma tecnologia revolucionária de encapsulamento que confere aos microrganismos (bactérias, leveduras, fungos) e enzimas características únicas necessárias para novas aplicações e inovações em bioprocessos.
Confira detalhes na vitrine de tecnologias da BiorrefinariaTec, uma plataforma de inovação aberta especializada no desenvolvimento das Biorrefinarias.
www.biorrefinariatec.com/vitrine
Microbial Diversity: Tapping the Untappedsachhatre
The document summarizes research on exploring microbial diversity to identify novel microbial functions. It describes enrichment and isolation of hydrocarbon-degrading bacteria from oil sludge to create a bacterial consortium for bioremediating oil spills. Molecular tools were used to characterize the isolates and determine their catabolic pathways. The consortium showed improved degradation of crude oil and model petroleum compounds compared to individual isolates.
This document discusses several papers that apply machine learning and multi-omics data to predict metabolic pathway dynamics.
- One paper develops a machine learning approach using proteomics data instead of kinetic modeling to accurately predict metabolite concentrations. This approach provides faster development of predictive models since it infers knowledge from data rather than requiring domain expertise.
- Another paper applies machine learning and multi-omics data to quantitatively predict production of the biofuel isopentenol from limited training data.
- A third paper reviews how knowledge engineering and data-driven frameworks using machine learning can offer new constraints for mechanistic models to better describe cellular regulation and design metabolic pathways. This facilitates "learn and design" for strain development.
Synthetic Biology for Plant ScientistsSachin Rawat
Tools of synthetic biology can be utilised to engineer metabolic pathways to optimize production of secondary metabolites and ligno-cellulose. The presentation describes an approach to develop an artificial positive feedback loop to increase accumulation of cell wall polysaccharides. These will decrease the cost of production of plant-based biofuels, paper and other plant products.
This document summarizes research on improving Pseudomonas putida KT2440 for the conversion of lignin-derived aromatic compounds into biofuels and bioproducts. Adaptive laboratory evolution was used to evolve P. putida strains with improved tolerance to the hydroxycinnamic acids p-coumaric acid and ferulic acid. Evolved strains showed significant decreases in lag phase and increases in growth rate in high concentrations of these acids. Whole genome sequencing identified genetic mutations conferring these improvements, including in genes like PP_3350 and ttgB. Deletion of these genes in the wild type strain verified their roles in tolerance. This work identifies new targets for engineering P. putida's ability to utilize aromatic compounds
The document discusses membrane bioreactor (MBR) technology for wastewater treatment. MBR combines a biological wastewater treatment process with a membrane filtration process. It provides several advantages over conventional activated sludge including higher quality effluent with very low levels of contaminants, complete pathogen removal, and ability to reuse treated water. The document examines various MBR configurations, design considerations, operating parameters, case studies on MBR use in antibiotic manufacturing wastewater treatment, and concludes that MBR is an effective technology for wastewater treatment applications.
This document discusses bioenergy and various bioenergy crops. It begins by defining bioenergy as renewable energy from biological sources like biomass, agricultural waste, and municipal waste. Key bioenergy crops discussed include sorghum, sugarcane, switchgrass, and miscanthus. The document outlines India's current bioenergy production and promising crops for ethanol and biodiesel. It discusses challenges in breeding perennial bioenergy crops and strategies to improve traits like yield, stress tolerance, and lignin content. Overall, the document presents bioenergy as a renewable and sustainable future fuel that can provide energy security while managing agricultural waste.
The study developed stochastic models to analyze the economic and environmental impacts of biodiesel production from Jatropha curcas in Nepal. The models determined that a minimum seed yield of 3.9 tons/hectare and oil content above 50% by weight are required for the biodiesel selling price to be comparable to conventional diesel prices. Including direct and indirect land use changes, the carbon footprint of Jatropha biodiesel could be lower than conventional fuels if high seed yields above 5 tons/hectare are achieved using only marginal lands and with aggressive afforestation. The results identify pathways for policies to enable sustainable Jatropha biodiesel production.
This document summarizes four research articles from the Office of Biological and Environmental Research.
The first article compares pretreatment processes using two ionic liquids to pretreat aspen and maple biomass for biofuel production. It finds one ionic liquid increased crystallinity while the other reduced it.
The second isolates five new Pseudomonas strains from soil that can metabolize pentose sugars, with genomes sequenced. This expands options for converting biomass into fuels and chemicals.
The third engineers poplar trees to produce less lignin using a bacterial enzyme, making the biomass easier to break down into sugars for biofuels. It reduces lignin by up to 30% while incorporating cleavable bonds into the lignin.
In this world of concerns regarding depletion of fossil fuels, pollution control and other factors leading to threat of man kind survival a way of producing biodiesel from algae which can be a source of alternative fuel. Lots of methods and sources being used for producing biodiesel but from algae one can produce high amount of biodiesel depending on the type of species or strain selected and this way this is a viable and feasible method to produce biodiesel.....
This document summarizes three papers related to engineering plant metabolism and microbial physiology.
The first paper describes engineering the brassinin biosynthetic pathway in plants to produce novel antifungal compounds called crucifalexins. Two of the new compounds showed stronger antifungal activity than brassinin.
The second paper discusses using solid-state NMR to characterize the native architecture of plant cell walls without disruption. This provides information to engineer bioenergy crops.
The third paper combines mechanistic and machine learning models to predictively engineer and optimize tryptophan metabolism in microbes. This approach increased tryptophan titer and productivity by up to 74% and 43% compared to an improved reference strain.
This study analyzed changes in the bacterial and fungal microbiome of soil samples treated with biosolarization, which uses solar heating and organic amendments. Sequencing analysis found that biosolarization had a stronger impact on the relative abundance of bacterial phyla than fungi. Network analysis identified microbial clusters correlated with volatile fatty acid accumulation, suggesting genera like Clostridium, Weissella and Acetobacter can tolerate and potentially produce these compounds. The results provide insight into structural changes in the soil microbiome during biosolarization as related to volatile fatty acid levels.
This study developed genetic tools for the thermophilic fungus Thermoascus aurantiacus, including a transformation system, CRISPR/Cas9 gene editing, and sexual crossing protocol. The transformation system was used to overexpress a transcriptional regulator of xylanase, increasing xylanase activity by up to 500%. CRISPR/Cas9 was shown to successfully delete a target gene with 10-35% efficiency. A sexual crossing protocol allowed crossing and isolation of progeny within a week, enabling strain engineering in this industrially relevant fungus. These tools open new possibilities for engineering thermostable enzyme production in T. aurantiacus.
Investing in Process Development for Increased MSC Production in Stirred Tank...MilliporeSigma
Interested in developing a robust cell therapy manufacturing platform? In this webinar we will share information in the form of case studies that highlight strategies to optimize your cell therapy production process.
Industry trends in regenerative medicine highlight a critical need for closed cell culture systems that support scalable manufacturing of adherent cell therapies. Typical static in vitro culture methods, however, are often too cumbersome and inefficient to support commercial scale production of mesenchymal stem/stromal cells (MSCs). Single-use stirred tank bioreactor systems are a platform that can address this limitation and have been proven effective for microcarrier-based production of adherent cell therapies. Implementation of optimized process control strategies for parameters such as dissolved oxygen (DO) and agitation rate are key to making an efficient transition from planar culture to stirred tank bioreactors. Herein, a stepwise approach to process development for MSC expansion in a small-scale single-use bioreactor is presented. Case studies focus on strategies to optimize DO control and agitation rates for bone marrow derived MSCs in microcarrier culture, highlighting improvements in process efficiency. In the first case study, the impact different gassing methods have on DO control and whether hypoxic growth conditions affect MSC function are examined. The second case study demonstrates the application of Zwietering’s equation for suspension of solids to overcome scaling challenges often associated with microcarrier culture in stirred tanks. Strategies to further improve the seeding process for bioreactor culture will also be reviewed. Identifying optimal seeding and process control strategies for microcarrier-based bioreactor expansion of adherent cells is paramount for the development of robust cell therapy manufacturing platforms.
In this webinar, you will learn about:
· Process development approaches for production scale-up of mesenchymal stem cells (MSCs)
· Implementing single-use, closed systems for manufacturing cell therapies
· Case studies focusing on strategies to optimize DO control and agitation rates for microcarrier-based cultures
Investing in Process Development for Increased MSC Production in Stirred Tank...Merck Life Sciences
This document discusses process development for manufacturing mesenchymal stem cells (MSCs) using microcarrier-based suspension culture in bioreactors. It outlines challenges in scaling up MSC production, including maintaining optimal hydrodynamic conditions and controlling dissolved oxygen. A case study demonstrates that MSCs expanded at 50L scale on microcarriers in a bioreactor retain their multipotent characteristics. The document also proposes strategies for optimizing a closed seed train to decrease manufacturing risks and costs compared to traditional planar seeding methods.
The document describes a proposed commercialization process for Miscanthus (Miscanthus x Giganteus) as a viable bioenergy crop in the United States. [1] It presents an integrated risk-balanced process model involving propagation of Miscanthus plantlets using automated nursery and greenhouse production, farming of the crop, and densification of the harvested biomass. [2] Key aspects of the model include using vegetative propagation to multiply plantlets at lower cost, implementing product mix strategies to reduce market risk during initial ramp-up, and precision planting techniques to reduce establishment costs. [3] The approach aims to make Miscanthus a profitable and scalable energy crop that can supply feedstock for cellulos
Event / Evento: II Workshop on Sugarcane Physiology for Agronomic Applications
Speaker / Palestrante: Gaspar H. Korndörfer (Federal University of Uberlândia)
Date / Data: Oct, 29-30th 2013 / 29 e 30 de outubro de 2013
Place / Local: CTBE/CNPEM Campus, Campinas, Brazil
Event Website / Website do evento: www.bioetanol.org.br/sugarcanephysiology
Event / Evento: II Workshop on Sugarcane Physiology for Agronomic Applications
Speaker / Palestrante: Jorge Donzeli (Sugarcane Research Center - CTC)
Date / Data: Oct, 29-30th 2013 / 29 e 30 de outubro de 2013
Place / Local: CTBE/CNPEM Campus, Campinas, Brazil
Event Website / Website do evento: www.bioetanol.org.br/sugarcanephysiology
Dr. Renato Vicentini from the State University of Campinas presented his research using systems biology approaches to understand sucrose synthesis and accumulation in sugarcane. His laboratory investigates gene regulatory, metabolic, and protein networks in sugarcane. They are developing predictive models to scale from genotype to phenotype. Their goals are to understand how some sugarcane genotypes accumulate more sucrose than others and to investigate allosteric regulation of key enzymes. Their approaches include RNA sequencing, metabolic profiling, and phosphoproteomics. They are also manipulating source-sink relationships in sugarcane to study differential gene expression and developing a sugarcane transcriptome. The talk provided an overview of their work using multi-omics data to build biological networks
Event / Evento: II Workshop on Sugarcane Physiology for Agronomic Applications
Speaker / Palestrante: Frederick C. Botha (Sugar Research Australia)
Date / Data: Oct, 29-30th 2013 / 29 e 30 de outubro de 2013
Place / Local: CTBE/CNPEM Campus, Campinas, Brazil
Event Website / Website do evento: www.bioetanol.org.br/sugarcanephysiology
This document provides an introduction to biological network inference using Gaussian graphical models. It discusses motivations for network inference based on the central dogma of molecular biology and common questions in functional genomics. The challenges of modeling high-dimensional omics data are described, including what network nodes and edges represent statistically and biologically. Gaussian graphical models are proposed as a tool for modeling dependencies between biological variables in genomic data, with the goal of reconstructing biological networks from large-scale omics experiments.
O documento estabelece as diretrizes para parcerias entre o CTBE e a indústria, visando:
1) Atrair parceiros capitalistas e transferir tecnologias;
2) Definir claramente as regras para projetos conjuntos, como propriedade intelectual, sigilo e divisão de riscos e benefícios;
3) Maximizar os ganhos de ambas as partes por meio de uma relação colaborativa.
O documento discute mecanismos para aproximar o CTBE da indústria, como a composição de um Comitê de Inovação e critérios de seleção de projetos de pesquisa. Também aborda a política de sigilo, regras de partilha de benefícios em parcerias, uso de quotas societárias e propriedade intelectual em projetos conjuntos.
1. O documento propõe a substituição de 10% da gasolina mundial por etanol brasileiro até 2025, o que traria grandes benefícios econômicos e sociais ao Brasil.
2. Seriam criados mais de 9 milhões de empregos, haveria um aumento de 13% no PIB brasileiro atual e seriam construídas 1000 novas destilarias.
3. Para isso, o Brasil criaria um laboratório nacional de pesquisa sobre bioetanol capaz de enfrentar os desafios tecnológic
Apresentação de Gilson Spanemberg realizada no "Workshop sobre Procedimentos que Regem o Relacionamento do CTBE com a Indústria"
Data: 1 de junho de 2010
Local: CTBE, Campinas, Brasil
Website do evento: http://www.bioetanol.org.br/workshop6
Apresentação de Laercio de Sequeira realizada no "Workshop sobre Procedimentos que Regem o Relacionamento do CTBE com a Indústria"
Data: 1 de junho de 2010
Local: CTBE, Campinas, Brasil
Website do evento: http://www.bioetanol.org.br/workshop6
Presentation of Arnaldo Walter for the "2nd Workshop on the Impact of New Technologies on the Sustainability of the Sugarcane/Bioethanol Production Cycle"
Apresentação de Arnaldo Walter realizada no "2nd Workshop on the Impact of New Technologies on the Sustainability of the Sugarcane/Bioethanol Production Cycle "
Date / Data : Novr 11th - 12th 2009/
11 e 12 de novembro de 2009
Place / Local: CTBE, Campinas, Brazil
Event Website / Website do evento: http://www.bioetanol.org.br/workshop5
Presentation of Celso Manzato for the "2nd Workshop on the Impact of New Technologies on the Sustainability of the Sugarcane/Bioethanol Production Cycle"
Apresentação de Celso Manzato realizada no "2nd Workshop on the Impact of New Technologies on the Sustainability of the Sugarcane/Bioethanol Production Cycle"
Date / Data : Novr 11th - 12th 2009/
11 e 12 de novembro de 2009
Place / Local: CTBE, Campinas, Brazil
Event Website / Website do evento: http://www.bioetanol.org.br/workshop5
The document discusses the Canasat Project, which uses remote sensing satellite images to monitor sugarcane crops in Brazil. It summarizes the project's use of satellite data to estimate sugarcane area, identify expansion and renovation, track pre-harvest burning, and analyze land use change. Spatial-temporal analysis of MODIS images from 2000-2008 showed land use changes from pasture to agriculture to sugarcane. The project is also developing models and research to better understand indirect land use change from sugarcane expansion.
O documento fornece informações sobre a produção de cana-de-açúcar no Brasil em 2008-2009, incluindo área colhida, produção, rendimento e produção de etanol. Também discute a contribuição de energia fóssil no processo e as emissões de gases de efeito estufa associadas.
Presentation of Manoel Regis Leal for the "2nd Workshop on the Impact of New Technologies on the Sustainability of the Sugarcane/Bioethanol Production Cycle"
Apresentação de Manoel Regis Leal realizada no "2nd Workshop on the Impact of New Technologies on the Sustainability of the Sugarcane/Bioethanol Production Cycle "
Date / Data : Novr 11th - 12th 2009/
11 e 12 de novembro de 2009
Place / Local: CTBE, Campinas, Brazil
Event Website / Website do evento: http://www.bioetanol.org.br/workshop5
Presentation of Manoel Regis Leal for the "2nd Workshop on the Impact of New Technologies on the Sustainability of the Sugarcane/Bioethanol Production Cycle"
Apresentação de Manoel Regis Leal realizada no "2nd Workshop on the Impact of New Technologies on the Sustainability of the Sugarcane/Bioethanol Production Cycle "
Date / Data : Novr 11th - 12th 2009/
11 e 12 de novembro de 2009
Place / Local: CTBE, Campinas, Brazil
Event Website / Website do evento: http://www.bioetanol.org.br/workshop5
Presentation of Martin Junginger for the "2nd Workshop on the Impact of New Technologies on the Sustainability of the Sugarcane/Bioethanol Production Cycle"
Apresentação de Martin Junginger realizada no "2nd Workshop on the Impact of New Technologies on the Sustainability of the Sugarcane/Bioethanol Production Cycle "
Date / Data : Novr 11th - 12th 2009/
11 e 12 de novembro de 2009
Place / Local: CTBE, Campinas, Brazil
Event Website / Website do evento: http://www.bioetanol.org.br/workshop5
Presentation of Marcia Azanha for the "2nd Workshop on the Impact of New Technologies on the Sustainability of the Sugarcane/Bioethanol Production Cycle"
Apresentação de Marcia Azanha realizada no "2nd Workshop on the Impact of New Technologies on the Sustainability of the Sugarcane/Bioethanol Production Cycle "
Date / Data : Novr 11th - 12th 2009/
11 e 12 de novembro de 2009
Place / Local: CTBE, Campinas, Brazil
Event Website / Website do evento: http://www.bioetanol.org.br/workshop5
Presentation of Marcel Gomes
for the "2nd Workshop on the Impact of New Technologies on the Sustainability of the Sugarcane/Bioethanol Production Cycle"
Apresentação de Marcel Gomes realizada no "2nd Workshop on the Impact of New Technologies on the Sustainability of the Sugarcane/Bioethanol Production Cycle "
Date / Data : Novr 11th - 12th 2009/
11 e 12 de novembro de 2009
Place / Local: CTBE, Campinas, Brazil
Event Website / Website do evento: http://www.bioetanol.org.br/workshop5
Presentation of Dr Mairi J Black
for the "2nd Workshop on the Impact of New Technologies on the Sustainability of the Sugarcane/Bioethanol Production Cycle"
Apresentação de Dr Mairi J Black realizada no "2nd Workshop on the Impact of New Technologies on the Sustainability of the Sugarcane/Bioethanol Production Cycle "
Date / Data : Novr 11th - 12th 2009/
11 e 12 de novembro de 2009
Place / Local: CTBE, Campinas, Brazil
Event Website / Website do evento: http://www.bioetanol.org.br/workshop5
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Biofuels and Biomaterial Research in the Porter Alliance
1. Biofuels and biomaterial
research in the Porter Alliance
Dr Richard Murphy
Imperial College London
Commercial in confidence – Imperial College London 2008
2. Structure
• Introduction to the Porter Alliance
• Biomass yield
• Biomass ‘quality’
• Sustainability and policy
• Closing remarks
Commercial in confidence – Imperial College London 2008
3. The alliance
with colleagues at Southampton University,
York University and University of Cambridge
Over 130 scientists, engineers,
economists and policy experts.
4. Mission
Devise economically, socially and
environmentally sustainable routes to the
production of energy and materials from
plants with a positive impact on climate
change and energy security.
7. Backing ligno-cellulosics
• 80% of biomass is in lignin and cellulose
• Perennial crops have low inputs and can
support higher levels of biodiversity
• If we could get at the sugar locked up in
cellulose, the current world motor fuel
energy consumption (1020 J/yr) might be
met from 125 M ha (10% of global arable
land)
8. The integrated biorefinery
Chain
Photosynthetic
Solar radiation Efficiency Useful energy
Efficiency
100 Wm-2 ~ (25%) 0.25 Wm-2
~ (1 %)
Scope for 2-fold Scope for 3-fold
Improvement improvement
Dunnett and Overall: scope for 6-fold improvement!
Shah J.
Biobased
Mater. Bio. 1
(2007)
9. Consider the whole process chain
BM 1
FEP 1 SC 1
BM 2 PC 1
FEP 2 SC 2
BM 3 PC 2
FEP 3 SC 3
BM 4 PC 3
FEP 4 SC 4
BM 5 PC 4
FEP 5 SC 5
BM 6
LEARNING
Biomass Front-end Primary Secondary
Classes Processes Conversions Conversions
10. Costs will improve with R&D and
commercialisation
From: The Royal Society report - Sustainable biofuels 2008
11. The essential messages
• There is a lot of headroom to make truly
sustainable lignocellulosic biofuel
• You must look at integrated processes to
achieve this
• We need to generate knowledge that will
guide us in choosing the best processes
12. Why the optimism ?
1. Tractable R&D challenges and
opportunities
2. Significant reductions in GHG emissions
are possible
3. Assuring sustainable land use
4. Many countries/regions can participate
13. Structure
• Introduction to the Porter Alliance
• Biomass yield
• Biomass ‘quality’
• Sustainability and policy
• Closing remarks
14. Biofuel crops and biomass sources
are diverse
Recognized interest
Wheat (grain and straw) Willows (SRC)
Oil seed Rape Poplars (SRC)
Sugar Beet Miscanthus
Sugar cane Biomass forestry
Sweet sorghum
Cassava Forestry/ processing residues
Jatropha Post-consumer ‘waste’ biomass
Future potential
Bamboo Coconut Algae
‘Novel’ (previously uncultivated) species
15. Approaches to achieving
higher yields
Development pipeline for biofuel crops
Increase yield Combat risks & limits
• Growth & architecture • Marginal land
• Duration of production • Resources
• Selection & breeding • Climate change
• Novel crops • Pests
• Carbon capture • Diseases
Integrated with sustainability and processing knowledge
17. Accelerating biomass yield in
Willow
VIc
0.0 Ea taM aa g2 4
5.1 MAX1
MnHt03LARS
MxDia03LARS
MnHt05RRes
MxDia06LARS
1 8.9 MAX4
2 4.3 W 11 47
2 4.4 W 98 8
Many thin stems Fewer thick stems 4 3.4 fEa c tM a ac 1 0 4
5 0.5 VI_ 5c
5 8.4 fEa tc M a at_ 20 9
The more axillary branching (max) mutants in
Arabidopsis have altered branching. Corresponding
genes map to yield QTL in willow.
18. Unique well established germplasm collections
1,300 accessions of willow (incl. 100 pure species) at Rothamsted Research
500 diverse poplars capturing wide natural diversity
Extensive
perennial grass
collections
including
800 accessions
of Miscanthus @
Rothamsted and
IBERS
19. Yield projections and modelling
Example data on poplar locations/yield - TSEC-Biosys Project
from:- Matt Ayott, Gail Taylor
Southampton University
Productivity map of Populus EC FP7 Project
trichocarpa genotype ‘trichobel’,
second rotation
20. Model plants & systems biology
Arabidopsis Targets for QTL
genomics Knowledge base
Poplar proteomics
Gene
discovery
metabolomics Data integration
Maize
high resolution Prediction tools
sampling
Targets for
Brachypodium molecular breeding
21. New leads for improving
biomass yield
knockout
Thorsten Hamann
knockout wT wT
The mutated gene is implicated The mutated gene
in response to pathogen infection encodes a UDP
glycosyl transferase
22. Structure
• Introduction to the Porter Alliance
• Biomass yield
• Biomass ‘quality’ and conversion
• Sustainability and policy
• Closing remarks
23. More quantity is only part of the solution
Increased and sustainable yield
• Optimise cell wall composition
– Systems biology approach
– High throughput analytics
– Regulating cell wall phenolics
– Self-processing plants ?
Increased and sustainable yield + optimised processability
24. and… big does not necessarily
mean sweet
with Rothamsted Research
Nick Brereton
Total Glucose Yield (g) / Oven Dry Weight (g)
- enzymatic hydrolysis, no pre-treatment
25.00%
20.00%
15.00%
10.00%
5.00%
An example in Willows 0.00%
Miscanthus 7 month Tora 2yr old Bowles Hybrid 3yr old
old
Bowles hybrid releases its glucan
High Biomass Medium Biomass
much more readily than other Yielding Willow Yielding Willow
varieties, even though it does not
produce the greatest mass
25. Willows contd.
Natural variation is large in saccharification and ethanol potential yield.
0 Ethanol ltr ha-1 without Pretreatment
J 1
orr
0 NOTE:
No pre-treatment,
Calculated ethanol yield
J 9
orr
0
the ‘inherent’
0
Bowles Hybrid enzymatic sugar
release is being
0
investigated here
0
0
Willow genotype
26. Miscanthus giganteus
a b
July to
December
c d
e f
During its annual growth there are large
developmental changes in Miscanthus .
How do these relate to saccharification ?
g h
by Muhammad Umer Ijaz PhD student,
with Rothamsted Research
Pl
27. Miscanthus giganteus contd.
Also:-
• variation
with internode
• fluctuation in
Starch content
Saccharification potential (no pre-treatment) changes substantially over the
development cycle
Harvesting time influences ease of enzymatic hydrolysis
Harvesting time is dictated by many constraints
28. Microbes that release sugar
from cell walls – pre-treatment
with
Mike Ray, Porter
Institute Research Fellow
and David Leak
and Pietro Spanu
We use fungi that
depolymerise the wood
cell wall
29. Microbial pre-treatment contd.
from pine sapwood
• Up to 70% of glucan becomes
available for enzymatic hydrolysis
• Ferments to ethanol without
inhibition
• No harmful waste streams
• Low energy inputs
• Little GHG emission
30. Experimental issues – Particle size
Effect of particle size on glucose yield
45
Glucose yield/ % ODW
40
35 >2000 µM
30 850-2000 µM
25
420-850 µM
20
15 250-420 µM
10
180-250 µM
5
0 100-180 µM
with Dr Mike Ray, Porter
Institute Research Fellow
31. Experimental – Enzymatic hydrolysis
• NREL recommends 96-168 hours
• Most papers promoting high-throughput
suggest 24 hours as sufficient
with Dr Mike Ray, Porter
Institute Research Fellow
32. Experimental – Enzymatic hydrolysis
Effect of incubation time on sugar yield
25
Glucose yield/ % ODW
20 Pine
Spruce
15
Willow O
10
96 144 168
5 72
6 24
0 1
0 50 100 150
Time/ hours with Dr Mike Ray, Porter
Institute Research Fellow
33. Structure
• Introduction to the Porter Alliance
• Biomass yield
• Biomass ‘quality’
• Sustainability and policy
• Closing remarks
34. Positively influencing GHG
and soil carbon balances
Understanding ‘Direct’ & ‘Indirect Effects’
– Read (2007)
Not all land use
– Searchinger et al + Fargione et al (2008)
change has to be
– Galbraith (2005)
‘negative’
from Dr Jem Woods, Porter Institute
35. Land availability
Country Population Total Land Arable land Land Considered Suitable % Suitable % of
for Crop Growth suitable
used
(2001-2005) - no - with
constraints - constraints -
2005
(people) (1000 ha) (1000 ha) (1000 ha) (1000 ha) (%) (%)
Brazil 186,831 853,363 58969 239,573 614,064 28% 25%
China 1,312,979 934,949 142265 178,228 756,722 19% 80%
India 1,134,403 306,140 159712 139,357 166,783 46% 115%
Southern Africa
Tanzania 38,478 93,819 9118 35,964 57,855 38% 25%
South Africa 47,939 122,300 14753 31,154 91,075 25% 47%
Mozambique 20,533 79,854 4270 48,043 31,811 60% 9%
Zambia 11,478 74,837 5260 22,304 52,533 30% 24%
Angola 16,095 123,776 3200 40,383 83,313 33% 8%
UK 60,245 24,418 5728 9,888 14,530 40% 58%
South East Asia
Indonesia 226,063 189,220 22600 79,444 109,776 42% 28%
Malaysia 25,653 33,300 1800 16,495 16,805 50% 11%
Total 3,080,697 2,835,976 427,675 840,833 1,995,267 30% 51%
World 6,515,000 12,976,000 3,500,000
from Dr Jem Woods, Porter Institute
36. Use of LCA in Porter Alliance Biofuels
R&D
Sustainability and life
Sustainability and life
cycle analysis
cycle analysis
Fungi
Fungi Butanologenic
Butanologenic
• Complexity of R&D
Rumen microbes
Rumen microbes
recombinant
recombinant
bacteria
bacteria opportunities and
Miscanthus
Miscanthus
Willow
Willow
Ionic liquids
Ionic liquids Long chain alkane //
Long chain alkane
alkanol producing
alkanol producing
possibilities – use process
Switchgrass
Switchgrass
Developmental front
Developmental front
end processes
end processes
organisms
organisms
systems engineering and
Poplar
Poplar Direct fermentation
Direct fermentation
sustainability modelling
Dilute acid // alkaline
Dilute acid alkaline of oligosaccharides
of oligosaccharides
Sugar cane bagasse
Sugar cane bagasse
Forest residues
Forest residues
Mild thermal
Mild thermal Developmental
Developmental
microbial
microbial
• LCA (+ other tools) to
find the most
Hydrothermal
Hydrothermal ethanologens
ethanologens
Crop residues
Crop residues
Steam
Steam
Thermochemical
Thermochemical
Proprietary
Proprietary
microbial
microbial
ethanologens
ethanologens
environmentally
sustainable routes
ENERGY CROPS
ENERGY CROPS FRONT END
FRONT END PRIMARY
PRIMARY
PROCESSES
PROCESSES CONVERSION
CONVERSION
Optimising yield
Optimising yield
Optimising
Optimising Optimising
Optimising
accessible carbon
accessible carbon conversion to
conversion to
biofuel
biofuel
37. Uses of LCA in policy – UK RTFO
• The UK Renewable Transport Fuels Obligation
(RTFO) provides a mechanism to support the
use of sustainable biofuels in the UK market
• It assesses greenhouse gas emissions and other
sustainability-linked criteria in an LCA context
• The first Quarterly Report on this by the
Renewable Fuels Agency was published in
October 2008
see http://www.renewablefuelsagency.org/
38. Supply chains and boundaries in the UK RTFO
process
Alternative Assessed ex post by
land use RTFO Administrator
Boundary for monthly
Previous Cultivation & carbon intensity calculation
land use harvest
Feedstock Biofuel Biofuel
Biofuel use
transport production transport
Waste
material
Excludes minor sources, from:
Alternative • Manufacture of machinery or
waste equipment
management • PFCs, HFCs, SF6
Assessed separately
Fossil fuel reference system
E4TECH, 2007
39. UK RTFO 1st quarterly report
• Biodiesel dominates Data here are for whole blended fuel
• Major biodiesel suppliers USA, UK &
Germany
• Major bioethanol suppliers Brazil, UK
Note: data is for obligation year to date based on submitted monthly returns to the
RFA. Final audit of this data occurs annually and revisions to the data may occur
at any point up to that time. RFA will publish a comprehensive end of year dataset
40. UK RTFO 1st quarterly report
The methodology is indicating differential savings in GHGs –
this is expected on the basis of LCA studies
Note: data is for obligation year to date based on submitted monthly returns to the
RFA. Final audit of this data occurs annually and revisions to the data may occur
at any point up to that time. RFA will publish a comprehensive end of year dataset
41. UK RTFO 1st quarterly report
Overall GHG savings were 44% vs a target of 40%
Note: data is for obligation year to date based on submitted monthly returns to the
RFA. Final audit of this data occurs annually and revisions to the data may occur
at any point up to that time. RFA will publish a comprehensive end of year dataset
42. UK RTFO 1st quarterly report
A ‘qualifying environmental standard’ is an existing certification scheme
that meets an acceptable number of the seven RTFO sustainability
principles (fuels from ‘wastes’ automatically comply)
Note: data is for obligation year to date based on submitted monthly returns to the
RFA. Final audit of this data occurs annually and revisions to the data may occur
at any point up to that time. RFA will publish a comprehensive end of year dataset
43. Structure
• Introduction to the Porter Alliance
• Biomass yield
• Biomass ‘quality’
• Sustainability and policy
• Closing remarks
44. We also regard Integration as
essential to progress
Platform tools &
Unique resources Systems Biology technologies
Sustainability
Processing
Bio energy crops Knowledge base evaluation
Integrated Biofuels
Refinery
Optimised bioenergy
crops