How different diseases originate with slightest changes in proteins. Understanding ALS , Parkinson, Alzhiemer , Taupathies development which may lead to their treatment!
Investigating Chemical Chaperones that can improve the stability of Lysozymes...oyepata
Investigating Chemical Chaperones that can improve the stability of Lysozymes
under high thermal temperature.
Sabastine Aliyu Zubairu1, Joseph Oyepata Simeon2, Isaac Ralph Elon1, Mahdi
Mohammed1, Sunday Blessing
How different diseases originate with slightest changes in proteins. Understanding ALS , Parkinson, Alzhiemer , Taupathies development which may lead to their treatment!
Investigating Chemical Chaperones that can improve the stability of Lysozymes...oyepata
Investigating Chemical Chaperones that can improve the stability of Lysozymes
under high thermal temperature.
Sabastine Aliyu Zubairu1, Joseph Oyepata Simeon2, Isaac Ralph Elon1, Mahdi
Mohammed1, Sunday Blessing
Background and objectives: the 5’nucleotidase (5’NT) is one of
hydrolytic enzymes present in different organs which catalyze
hydrolysis of 5’ nucleotides to ribonucleosides and phosphate.
Malondialdehyde (MDA) is the end product of lipid peroxidation
by oxidative stress (free radicals).
The aim of present study was to measure the serum activity of
5’NT, and MDA concentration in breast tumors.
Material and method: A prospective study was carried out from
May to December 2013 by clinical biochemistry department in
College of Medicine-Hawler Medical University on (30) healthy
female individuals, (group 1) and (30) females with breast tumor
(group 2).
Results: The mean value of serum MDA was significantly higher
in females with breast tumors (group 2), than that of healthy
female individuals, (group 1) (p<0.01),><0.01).
Conclusion: Based on findings of the present study it can be
concluded that breast tumors can cause release of the enzyme
5’NT from tumor cells, and lipid peroxidation by reactive oxygen
species (ROS), which cause elevation of MDA.
Therapeutic Glycoproteins are budding medicine for the treatment of various life threatening diseases like cardiometabolic disorders, autoimmune diseases and cancer. Most of the approved therapeutic proteins are produced in Chinese Hamster Ovary (CHO) cell line.
Its increasing demand has challenged the current production capacity. So as to increase the quality and quantity of these glycoproteins various attributes are being exploited such as the glycosylation patterns which is affected by the cell culture media components, manufacturing mode, culture system and operation, pH, temperature, different expression systems like mammalian, insect, plant, and animal expression system. The choice of a significant host system plays a crucial role in the resulting glycan produced. Glycosylation is highly regulated mechanism, a crucial protein quality attribute that can modulate the efficacy of therapeutic glycoprotein
In this slide contains definition, types, causes, inducers and inhibitors, complex drug interactions.
Presented by: SUMASHREE AGGIM (Department of pharmacology).
RIPER, anantapur
Molecular Modeling of Metalloreductase STEAP2 Protein and Docking Interaction...BRNSS Publication Hub
This gene is an individual from the STEAP family and encodes a multipass film protein that confines to the Golgi complex, the plasma layer, and the vesicular cylindrical structures in the cytosol. A very comparative protein in mouse has both ferrireductase and cupric reductase action and invigorates the cell take-up of both iron and copper in vitro. Expanded transcriptional articulation of the human quality is related with prostate malignant growth movement. Substitute transcriptional graft variations, encoding distinctive isoforms, have been described. Therefore, in the present study, we generated a precise three-dimensional (3D) model of metalloreductase STEAP2 protein using MODELLER 9.21 and validated its structure using PROCHECK software. Modeled protein contains more than 94.5% of amino acids in core region. We interpreted the action of natural compounds docking against the modeled metalloreductase STEAP2 protein. Three compounds (ginkgetin, medicagenin, and erybraedin A) showed lower binding affinity values toward metalloreductase STEAP2 protein compared to mitoxantrone, abiraterone acetate, apalutamide, enzalutamide, and flutamide. Ginkgetin exhibited the lowest binding energy of −9.10 kcal/mol with interacting Trp212 and Thr210. All the 17 compounds showed excellent binding energies than standard drugs for the modeled metalloreductase STEAP2 protein. These computational studies can be helpful to discover novel drug candidates.
Role of Antioxidants in the Treatment of Male Infertility-Crimson PublishersCrimsonpublisherssmoaj
Stress is defined as a general body response to initially threatening external or internal demands, involving the mobilization of physiological and psychological resources to deal with them. Recently, oxidative stress has become the focus of interest as a potential cause of male infertility. Normally, equilibrium exists between reactive oxygen species (ROS) production and antioxidant scavenging activities in the male reproductive organs. Non-hormonal medical treatment has been proposed for patients with idiopathic or non-curable oligo-astheno-terato-zoospermia and for normo-zoospermic infertile patients. Anti-inflammatory, fibrinolytic & antioxidant compounds, oligo elements and vitamin supplementation may be prescribed. Infection, inflammation and/or increased oxidative stress often require a specific treatment with antibiotics, anti-inflammatory drugs and/or antioxidants. The ascorbic acid is a known antioxidant present in the testis with the precise role of protecting the male reproductive organs from the oxidative damage. It also contributes to the support of spermatogenesis at least in part through its capacity to maintain antioxidant in an active state. Combined therapies can contribute to improve sperm quality.
In medicine, proteopathy refers to a class of diseases in which certain proteins become structurally abnormal, and thereby disrupt the fuction of cells, tissues and organs of the body. Often the proteins fail to fold into their normal configuration; in this misfolded state, the proteins can become toxic in some way (a gain of toxic function) or they can lose their normal function. The proteopathies (also known as proteinopathies, protein conformational disorders, or protein misfolding diseases), include such diseases as Alzheimer's disease, Parkinson's disease, prion disease, type 2 diabetes, amyloidosis, and a wide range of other disorders .
Background and objectives: the 5’nucleotidase (5’NT) is one of
hydrolytic enzymes present in different organs which catalyze
hydrolysis of 5’ nucleotides to ribonucleosides and phosphate.
Malondialdehyde (MDA) is the end product of lipid peroxidation
by oxidative stress (free radicals).
The aim of present study was to measure the serum activity of
5’NT, and MDA concentration in breast tumors.
Material and method: A prospective study was carried out from
May to December 2013 by clinical biochemistry department in
College of Medicine-Hawler Medical University on (30) healthy
female individuals, (group 1) and (30) females with breast tumor
(group 2).
Results: The mean value of serum MDA was significantly higher
in females with breast tumors (group 2), than that of healthy
female individuals, (group 1) (p<0.01),><0.01).
Conclusion: Based on findings of the present study it can be
concluded that breast tumors can cause release of the enzyme
5’NT from tumor cells, and lipid peroxidation by reactive oxygen
species (ROS), which cause elevation of MDA.
Therapeutic Glycoproteins are budding medicine for the treatment of various life threatening diseases like cardiometabolic disorders, autoimmune diseases and cancer. Most of the approved therapeutic proteins are produced in Chinese Hamster Ovary (CHO) cell line.
Its increasing demand has challenged the current production capacity. So as to increase the quality and quantity of these glycoproteins various attributes are being exploited such as the glycosylation patterns which is affected by the cell culture media components, manufacturing mode, culture system and operation, pH, temperature, different expression systems like mammalian, insect, plant, and animal expression system. The choice of a significant host system plays a crucial role in the resulting glycan produced. Glycosylation is highly regulated mechanism, a crucial protein quality attribute that can modulate the efficacy of therapeutic glycoprotein
In this slide contains definition, types, causes, inducers and inhibitors, complex drug interactions.
Presented by: SUMASHREE AGGIM (Department of pharmacology).
RIPER, anantapur
Molecular Modeling of Metalloreductase STEAP2 Protein and Docking Interaction...BRNSS Publication Hub
This gene is an individual from the STEAP family and encodes a multipass film protein that confines to the Golgi complex, the plasma layer, and the vesicular cylindrical structures in the cytosol. A very comparative protein in mouse has both ferrireductase and cupric reductase action and invigorates the cell take-up of both iron and copper in vitro. Expanded transcriptional articulation of the human quality is related with prostate malignant growth movement. Substitute transcriptional graft variations, encoding distinctive isoforms, have been described. Therefore, in the present study, we generated a precise three-dimensional (3D) model of metalloreductase STEAP2 protein using MODELLER 9.21 and validated its structure using PROCHECK software. Modeled protein contains more than 94.5% of amino acids in core region. We interpreted the action of natural compounds docking against the modeled metalloreductase STEAP2 protein. Three compounds (ginkgetin, medicagenin, and erybraedin A) showed lower binding affinity values toward metalloreductase STEAP2 protein compared to mitoxantrone, abiraterone acetate, apalutamide, enzalutamide, and flutamide. Ginkgetin exhibited the lowest binding energy of −9.10 kcal/mol with interacting Trp212 and Thr210. All the 17 compounds showed excellent binding energies than standard drugs for the modeled metalloreductase STEAP2 protein. These computational studies can be helpful to discover novel drug candidates.
Role of Antioxidants in the Treatment of Male Infertility-Crimson PublishersCrimsonpublisherssmoaj
Stress is defined as a general body response to initially threatening external or internal demands, involving the mobilization of physiological and psychological resources to deal with them. Recently, oxidative stress has become the focus of interest as a potential cause of male infertility. Normally, equilibrium exists between reactive oxygen species (ROS) production and antioxidant scavenging activities in the male reproductive organs. Non-hormonal medical treatment has been proposed for patients with idiopathic or non-curable oligo-astheno-terato-zoospermia and for normo-zoospermic infertile patients. Anti-inflammatory, fibrinolytic & antioxidant compounds, oligo elements and vitamin supplementation may be prescribed. Infection, inflammation and/or increased oxidative stress often require a specific treatment with antibiotics, anti-inflammatory drugs and/or antioxidants. The ascorbic acid is a known antioxidant present in the testis with the precise role of protecting the male reproductive organs from the oxidative damage. It also contributes to the support of spermatogenesis at least in part through its capacity to maintain antioxidant in an active state. Combined therapies can contribute to improve sperm quality.
In medicine, proteopathy refers to a class of diseases in which certain proteins become structurally abnormal, and thereby disrupt the fuction of cells, tissues and organs of the body. Often the proteins fail to fold into their normal configuration; in this misfolded state, the proteins can become toxic in some way (a gain of toxic function) or they can lose their normal function. The proteopathies (also known as proteinopathies, protein conformational disorders, or protein misfolding diseases), include such diseases as Alzheimer's disease, Parkinson's disease, prion disease, type 2 diabetes, amyloidosis, and a wide range of other disorders .
Protein glycosylation and its associated disordersSaranya Sankar
Protein glycosylation and its associate disorders. Glycosylation is one of the post translational modifications important for the normal function of the protein such as cell adhesion, signalling etc.. defect in this process leads to fatal disorder such as cancer, PNH....
Pharmacogenomics is a new trending branch which has created enormous hopes in improving diagnostic methods, treatment outcomes and preventing adverse events and therapeutic failures. In this ppt basics of pharmacogenomics and pharmacogenetics has been discussed in simplest possible way along with two case studies. Clinical applications of pharmacogenomics has also been discussed in brief.
International Journal of Clinical Pharmacology & Toxicology (IJCPT) ISSN:2167-910X is an Open Access journal, which aims to develop coherent means to modify drug therapy, with respect to the patient's genotype, and to ensure maximum efficiency with minimal contrary effects.
International Journal of Clinical Pharmacology & Toxicology (IJCPT) ISSN:2167-910X is an Open Access journal and a peer-reviewed journal. Clinical Pharmacology & Toxicology is the all-encompassing and becoming an increasingly important discipline for the identification of disease targets and drug designing with their toxicological effects and means to eradicate diseases.
In Silico Modeling and Docking Studies on Methionine Sulfoxide Reductase A Pr...BRNSS Publication Hub
Alzheimer disease (AD) is a neurodegenerative disorder including continuously progressive cognitive and functional deficits as well as behavioral changes and is related with amassing of amyloid and tau depositions in the brain. Subjective side effects of AD most ordinarily incorporate deficits in short-term memory, executive and visuospatial dysfunction, and praxis. Mammalian methionine sulfoxide reductase A is encoded by a single gene and is found in both cytosol and mitochondria. Biologically active compounds from different plants have been used to treat various ailments. In the present study, mitochondrial peptide methionine sulfoxide reductase protein sequence from Homo sapiens was retrieved from UniProt and selected structure of the peptide methionine sulfoxide reductase from Escherichia coli (Protein Data Bank [PDB] id: 1FF3) was used as template. The homology model was developed by using Modeller 9.20 version. Molecular docking studies were performed using Autodock4.2. 20 natural compounds were docked against modeled protein. All the compounds exhibited good binding energy. Campesterol showed with lesser energy of −9.0 Kcal/mol.
These are the abstracts indexed with my name in the Proceedings of the National Symposium on Evolving Paradigm to Improve Productivity from Dynamic Management and Value Addition for Plant Genetic Resources.
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
1. Isolation and Analysis of Glycosphingolipids, N-linked Glycans, and O-linked Glycans from a Single Sample of
Human Serum
Seth Burgess, Ashley Medeiros, David Ashline, and Vernon Reinhold
Department of Molecular, Cellular, and Biomedical Sciences
References
• Ruhaak LR, Zauner G, Huhn C, Bruggink C, Deelder AM, Wuhrer M. Glycan labeling
strategies and their use in identification and quantification. Analytical and
Bioanalytical Chemistry. 2010;397(8):3457-3481. doi:10.1007/s00216-010-3532-z.
• Xhang XL. Roles of glycans and glycopeptides in immune system and immune-related
diseases. Current Medicinal Chemistry. 2006; 13(10):1141-7.
• Fredonia.edu/bio241/images/5.19_ER_and_Golgi.jpg
• University of British Columbia Department of Zoology. Vancouver, British Columbia.
http://www.zoology.ubc.ca/~berger/B200sample/unit_8_protein_processing/golgi/lec
t28.htm
• Brooklyn College Department of Biology. Brooklyn, New York.
http://academic.brooklyn.cuny.edu/biology/bio4fv/page/pm_mos.htm
Introduction
Glycomics - The study of the glycome, or the
comprehensive collection of sugar elements
in an organism.
Glycan – A compound that contains an
oligomeric or polymeric number of
carbohydrate subunits.
In the cell, proteins are glycosylated in the
Golgi apparatus to result in a glycoprotein.
Based on the amino acid residue that the
sugar is attached to, this process creates
either an O-linked or an N-linked glycan.
N-linked glycan – Asparagine residue
O-linked glycan – Serine or Threonine residue
Glycolipids – Lipids with a carbohydrate
attached
Methods
Glycolipids were extracted from the sample
using sonication and centrifugation, leaving the
glycoproteins in the human serum sample in the
pellet.
Proteolysis using the enzymes trypsin and
chymotrypsin were employed to solubilize the
peptides in the collected pellet sample.
N-Glycanase enzymes were used to release N-
linked glycans from the proteins to which they
were attached, and these glycans were purified
from the remaining O-linked glycans via C18
Solid Phase Extraction.
The N-glycans were reduced using borane-
ammonia reduction methods and purified via
graphitized carbon cleanup.
The O-linked glycans were then released via ion
exchange including the addition of sodium
hydroxide, sodium borohydride, and acetic acid.
C18 solid phase extraction was used to purify
the sample of any residual borane from the
borane-ammonia reduction of the N-glycans in
the previous step.
Permethylation was conducted on both the N-
and O-linked glycans to aid in later MALDI mass
spectrometric analysis of the glycans present in
the human serum sample under investigation.
Glycoprotein Processing
During its synthesis in the rough endoplasmic reticulum, a
protein can be tagged with a mannose tree transferred
from a molecule called dolichol. This mannose tag
designates the protein for glycosylation in the Golgi
apparatus. Once it reaches the Golgi, the mannose tag is
removed and glycosylation of the protein can proceed to
form a functional glycoprotein.
Importance of Glycomic Discovery and
Research
* Genomics is the past, proteomics is the present, glycomics is the
future.
* Glycoproteins consist of the majority of compounds in immune
response. Foreign antigens are also glycoproteins.
* Glycomic elements add stability to proteomic elements.
* Glycopeptides play a key role in cell signaling and development.
* Glycopeptides allow for specific epitopes that allow immune
recognition responses to occur.
* Glycoproteins such as human serum in blood and blood plasma are
involved in ensuring proper circulation.
* Glycomic discovery opens up opportunities for personal medicine,
such as blocking viral attachment and immune system regulation.
Analysis of Results
• N-linked glycans were more diverse and
generally more abundant than O-linked
glycans. This means that the asparagine
residue is either more easily glycosylated or
more numerous in in human serum when
compared to serine or threonine.
• N-glycans generally have a higher m/z ratio.
• Our ratios are well calibrated, as more than
90% of our structures deviate less than 2 mass
units from the theoretical values.
• Our N and O-linked structures are found in the
same profile, which means that there may
have been some issues with our N-linked
release.