The document is a cover letter and resume from Kandunuri Upender applying for a job in the biotechnology department of an organization. In the cover letter, he indicates that he has heard the organization has a vacancy that matches his qualifications. His resume then outlines his educational and professional background in biotechnology, including a MTech degree, skills in various biotechniques, and work experience as a biotechnology lecturer. He provides references and seeks an opportunity for an interview.

1. K.Upender,,
C/o kandunuri kondaiah,,
Perikasingaram[PO],
Kusumanchi[MD]l,
Khammam – 507159.
Mobile No: 9908426978.
TO.
Respected sir,
Subject: To seeking a job in your organization, in Biotechnology Dept.
I have come to know through some reliable sources that you have a
vacancy in your renowned organization, since I meet all the required
qualification and conditions, I wish to offer my candidature for the same
and apply here under my CV relevant to the job. I assure you, if you
favoured me with an opportunity, I shall do my best to work to the entire
satisfaction of my superiors.
Wishing a favourable reply from your organization
Thanking you sir
Date: 02-09-2015 Yours Faithfully
Place: KHAMMAM (K.UPENDER)
CURRICULUM VITAE
KANDUNURI UPENDER up1771@gmail.com

2. OBJECTIVE :
• To be associated with a progressive organization that would give me scope to learn and
apply my knowledge and skills abreast with latest trends and to be a part of a team that
dynamically works towards the growth of the organization.
EDUCATIONAL QUALIFICATIONS:
--------------------------------------------------------------------------------------------------------------------
Qualification University/ Year Percen- Class
Institution Passed tage Obtained
-------------------------------------------------------------------------------------------------------------------Mtech
JNTU kakinada 2011 65.00% First class
biotechnology
M.Sc, Bharathidhasan 2009 70.00% First Class
Biotechnology University
B.Sc, Biotechnology, Kakatiya 2004 58.00% second Class
Chemistry, Botany University
-------------------------------------------------------------------------------------------------------------------
Other Qualification;
• DIPLOMA in Chemical Analasis and Quality Management in HCU.
• DIPLOMA IN Bioinformatics Bharatidasan University.
Biotechniques Familiar with:
• Biochemistry:
• Buffer preparation
• Extraction and estimation of macromolecules
1.Total protein
2.Total sugars
3.Reducing sugars
4.Fractionation of total lipid by column chromatography
• Colorimetric assay of enzyme activity
1.Amylase
2. Invertase
3. Protease

3. Molecular Biology & Tissue Culture:
• DNA extraction in Prokaryotes and Eukaryotes.
• Restriction Enzyme digestion of DNA.
• Isolation of Plasmid DNA.
• Isolation of plant genomic DNA (CTAB method).
• Isolation of genomic DNA from animal cells.
• Transformation and conjugation in E.coli.
• Organ culture: Shoot tip, nodal, leaf, Callus culture.
• Isolation of plant chloroplast.
• Isolation, culture and fusion of protoplasts.
• Preparation of artificial seeds.
• Polymerase chain reaction.
• Southern, northern, western Blotting
Microbiology & Immunotechniques:
• Staining techniques
• Blood group testing
• Rocket immuno electrophoresis
• Ouchterlony double diffusion
• Electrophoresis of immunoglobulins preparation
• Precipitation of immunoglobulins
• Well equipped in basic microbiological procedures
• Preparation of media (Whites, MS)
• ELISA
Bioprocess Engineering:
• Preparation of wine from Grapes.
• Production of Citric acid using AspergillusNiger.
• Production of Ethanol using Yeast.
Technical Skills:
Understanding in the basics and developments in
• HPLC,GPC,MS
• Plasmid and DNA isolation.
• Electrophoretic techniques.
• Microbial techniques.
• Molecular techniques.
• Sequencing

4. • Immunological techniques
• Plant Tissue Culture.
• Animal tissue culture
Project Details:
Project Title : CLINICAL RESERCH IN IJRRPAS PUBLICATION
Project Duration : One year
Organization : GOUTHAMI,GUNTUR.
Project Details : EVALUATION OF ADVANCED .DISEASE DIAGNOSTIC METHODSUSINGBY
NUCLEIC ACIDS.
Msc. Title : "isolation and identtification of amylase produsing bacteria from soil.
Technical Qualifications:
• WINDOWS and MS-Office.
• Internet surfing.
Other Information:
• Participated in National Seminar on Trends in Biotechnology on Sep. 24/ 26- 2011 held
at Dept. of Biotechnology ANU.
• Presented a paper Presentation, Evaluation of Serum arginase and Acid Phosphatase
levels in Human Breast Cancer. At Annamalai university on Mar. 10-2011
• Participated in National Seminar on Trends in Biotechnology in the 21st Century –
perspectives in Bioactive Molecules on Feb. 12/14-2001 held at Department of
Biotechnology, GITAM, A.U. Visakhapatnam, A.P. India.
• Attended National Seminar on “ EMERGING TRENDS ON BIOINFORMATICS ”
Conducted by the Department of Biotechnology, D.N.R College, and
Bhimavaram.A.P.India.
• Given a talk on Plant Tissue Culture Techniques at BIOTRIM, Tirupathi, and A.P.
India.
• Received a Runners (Single) Certificate in Table Tennis held by Andhra University,
College of Science & Technology, Waltair, Visakhapatnam.
• Participated in Shuttle Badminton at District Level.

5. Personal skills:
• Excellent communication skills in English, Telugu and Hindi.
• Motivated and enthusiastic
• Ability to learn new things and to pickup very fast.
• Highly inquisitive, creative and resourceful.
• Good working knowledge of Biochemistry, Microbiology, Biochemical
techniques and Immunology.
Work experience:
• Worked as a Biotechnology Lecturer for KAVITHA. Degree College , KHAMMAM,2012 June.
to 2015 April..
• BIOLOGICAL E QC DEPARTMENT 6MONTHS WORK EXPIRENCE.
REFERENCES:
1. Prof. P. Venkateswara rao
Biotechnology Division
College of Science and Technology
Gitam University
Visakhapatnam.
2. Dr. G. Trimurtulu
Vice president,
Laila Impex,
Jawahar Autonagar,
Vijayawada-52007
9440683112 9949010111
3. Prof.I. Bhaskar Reddy
HOD, Bio chemistry dept.
Gitam University,
Visakhapatnam.
9440119739

6. PERSONAL MEMORANDA:
• Name : Kandunuri Upender
• Father’s name : K.Kondaiah
• Date Of Birth : 20march 1982
• Gender : Male
• Languages Known : English, Hindi And Telugu
• Marital Status : Married
• Strengths : Leadership & Commitment.
• Address : K.Upender
C/o kandunuri kondaiah,
perikasingaram[PO]
Kusumanchi Mandal,
Khammam – 507159
• Contact No : 9908426978,
9553082676.
• E.mail Id : up1771@gmail.com
DECLARATION :
I hereby declare that the above-mentioned details are true to the best of my
knowledge and belief.
Thank you, for your time.
Yours faithfully

7. (KANDUNURI UPENDER)