Tnb ccompleytari 2
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Experimental preclinical models that resemble patient tumors can improve target identification and therapeutic efficacy. Patient-derived xenograft (PDX) models, due to their genomic and transcriptomic similarity to patient tumors, can improve preclinical testing of drug combinations. Despite previous development of breast PDX models, models derived from patients with triple negative breast cancer are lacking. One study applied hybrid imaging analysis to a PDX model of triple negative breast cancer to validate the method for use in clinical practice based on evaluated and validated mouse models. Limited utility of established tumor cell lines has made transplantable tumor xenograft models superior for refining preclinical models with new knowledge of tumor heterogeneity. A study analyzed gene expression in 93 cases of triple negative breast cancer
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Tnb ccompleytari 2
- 1. Experimentalpreclinicalmodelsthatrecapitulate theirorigintumorscanaccelerate targetidentification, thusimprovingtherapeuticefficacy.Patient-derivedxenografts(PDX),due totheirgenomicand transcriptomicfidelitytothe tumorsfromwhichtheyare derived,are able toimprove preclinicaltesting of targetdrug combinationsintranslational models.Despite previousdevelopmentof breastPDXand TNBC models,those derivedfrompatientswithdemonstratedtriple negativehealthtumorsare lacking. The use of hybridimaginginradimicanalysiswasappliedonaPDX model byTNBC,in orderto validate thismethodinclinical practice basedonmodelsevaluatedandvalidatedwiththe helpof mouse studies. The limitedutilityof tumorcell linesestablishedinrefiningpreclinicalmodelsinthe contextof new knowledge relatedtotumorheterogeneityhasmade itnecessarytouse transplantabletumor xenografts,patient-derived(PDX) modelsbeingsuperiorforthispurpose.A studythatanalyzedgene expressionof 93 TNBC inorder to correlate withthe five subtypesof TNBCaimedtoevaluate the reproducibilityof radiomicmetricsinPET-CTimaginginpreclinical settings.The methodincluded evaluatingtumorswithvolumesbetween100-450 mm3, recordingthe PET-CT image parametersusing 18F-FDG at 45-60 minpost-injection.The resultsare aimedatstandardizingthe acquisitionand extractionof the image characteristicsinordertocorrectlyidentifythe 5subtypesof TNBC. MatossianMD, BurksHE, ElliottS,etal.Drug resistance profilingof anew triple negativebreastcancer patient-derivedxenograftmodel.BMCCancer.2019 Mar 7;19(1):205. TimothyWhitehead,MadhusudanSavaikar,Lori Strong,etal.Reproducibilityof FDG-PETradiomic parametersfortriple negative breastcancer(TNBC) sub-types.JNucl MedMay 1, 2018 vol.59 no. supplement11757