Thin Layer Chromatography (TLC) is a technique used to separate moderately volatile or nonvolatile substances based on their differential adsorption onto an inert solid stationary phase immersed in an organic solvent or solvent mixture mobile phase. TLC was first introduced in 1938 and remains one of the simplest, fastest, easiest and least expensive chromatographic separation methods.

2. DEFINITION
Thin Layer Chromatography (TLC) is a technique to separation of moderately volatile or
nonvolatile substances based on differential adsorption on an inert solid (stationary phase)
immersed in a organic solvent or solvent mixture (mobile phase). TLC was first introduced
by Izmailov & Shraiber in 1938.
TLC is one of the simplest, fastest, easiest and least expensive of several chromatographic