there is no more info for this question other than what is posted here. When you performed your IP1 ELISA you included two controls along with your samples and standards. These were the NSB and TA wells. The plate reader printout of a hypothetical dataset for a competition ELISA is given below. Something went wrong with this ELISA assay, and no useful information can be interpreted from the absorbance numbers given. Which of the two controls (NSB or TA) is indicating a problem? Why?.