2. Listeria monocytogenes is a gram posative bacteria
which is an invasive pathogen in both humans and
animals
It is contracted through ingesting contaminated food
Once in the intestine it attatches to the epithelial cells
at 2 main sites
At E-cadherin and at Met
Met is located on the cell surface and usualy binds
HGF
Whilst E-cadherin is crutial to tight junction
formation between cells as it binds the two
cytoskeletons from each ajacent cell
3.
4. Once in the intestine L.Monocytogenes attatches to
the epithelial cells at 2 main sites
At E-cadherin and at Met
Met is located on the cell surface and usually binds
HGF
Whilst E-cadherin is crucial to tight junction
formation between cells as it binds the two
cytoskeletons from each adjacent cell
It binds these proteins through InIA and InIB
5.
6. Once bound a conformational change is produced by
CDC42 and Rac which activate N-wasp which alters
the proliferation of actin molecules
Bringing the bacteria into the cell which then used
host actin monomers to form actin tails which project
the bacteria through the cell
During this mass replication occurs
The bacteria can also use these actin tails to forse their
way into adjacent cells
