Studies on phyllosphere fungi. I. Effect of foliar application of plant extracts
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The effect of leaf extracts of Calotropis procera and Datura metal on phyllosphere microflora of Pennisetum typhoides has been investigation. The different concentrations of the leaf extracts of the two plants were tried. The extracts above 4% concentration in both cases proved inhibitory for certain phyllosphere fungi. The inhibitory property of leaf extracts of two plants, however, differed with regard to certain fungal species. The leaf extract of D. metal proved to be more effective than that of C. procera. The spore germination of certain fungal species was considerably lowered in the extract of the two plant species.
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Recent Advances in Integrated Pest Management of Sorghum
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Etude de la Phyllosphère
Présentation du projet de recherche réalisé au Biodôme de Montréal avec le soutien de l'Université de Clermont-Ferrand et l'IUT des Cezeaux.
Sclerotia production a way ahead to morchella Cultivation
Morchella, the true morels, belonging to Helvellaceae family of class
Ascomycetes, are amongst the most highly priced fungi in the world. Their
artificial production is still a challenge, even though patents for their
cultivation do exist. The tissue of Morchella sp. was transferred aseptically
to Potato Dextrose Agar medium (peeled, sliced and boiled potato, 200 g;
dextrose, 20 g; agar, 20 g L-1) to grow hyphae. The mycelium showed
fastest growth as compared to other edible mushrooms. It covered entire area
of Petri plate (90 mm) within 4-5 days with the growth rate of 18-
22.5mm/day. A unique growth pattern i.e., vertically oriented mycelia were
observed. Brown coloured pigmentation in the culture was also observed
during the study. The basal media for spawn (wheat grains; Glucose, 1%,
CaCO3, 2%; CaSO4, 1.5% and MgSO4, 1%) was aseptically inoculated with
the mushroom culture. The spawn substrate was colonized by mushroom
mycelium in 7-8 days. The sclerotia were formed in unused (old) spawn.
Wheat straw was used as a substrate for mushroom cultivation. It was
supplemented with wheat bran, 20%, Glucose (1%) and MgSO4 (1%). It
showed prolific growth when it was seeded by mushroom spawn using jar
method. Once substrate was fully covered with mushroom mycelium, casing
was applied. Sclerotia were successfully obtained after 14 days of incubation
in our experiment both in the substrate and casing soil but failed to give rise
to fruiting primordial. Further research is going on to domesticate this
species in our country.
Sclerotia production a way ahead to morchella cultivation
Morchella, the true morels, belonging to Helvellaceae family of class Ascomycetes, are amongst the most highly prized fungi in the world. Their artificial production is still a challenge, even though patents for their cultivation do exist. The tissue of Morchella sp. was transferred aseptically to Potato Dextrose Agar medium (peeled, sliced and boiled potato, 200 g; dextrose, 20 g; agar, 20 g L-1) to grow hyphae. The mycelium showed fastest growth as compared to other edible mushrooms. It covered entire area of Petri plate (90 mm) within 4-5 days with the growth rate of 18-22.5mm/day. A unique growth pattern i.e. vertically oriented mycelia were
observed. Brown coloured pigmentation in the culture was also observed during the study. The basal media for spawn (wheat grains; Glucose, 1%, CaCO3, 2%; CaSO4, 1.5% and MgSO4, 1%) was aseptically inoculated with the mushroom culture. The spawn substrate was colonized by mushroom mycelium in 7-8 days. The sclerotia were formed in unused (old) spawn. Wheat
straw was used as a substrate for mushroom cultivation. It was supplemented with wheat bran, 20%, Glucose (1%) and MgSO4 (1%). It showed prolific growth when it was seeded by
mushroom spawn using jar method. Once substrate was fully covered with mushroom mycelium, casing was applied. Sclerotia were successfully obtained after 14 days of incubation in our experiment both in the substrate and casing soil but failed to give rise to fruiting primordial. Further research is going on to domesticate this species in this part of country.
A new filter for safe view of solar eclipse
Looking directly at the sun can lead to permanent eye injury due to damage of light-sensitive rod and cone cells within the retina. There are two ways to look at the Sun safely: by observing the sun directly through a suitable filter, or by projecting the Sun’s reflection onto a piece of paper via handmade pinhole camera/ telescope. The present correspondence shows the specification for the development of an optical instrument for the direct observation of sun. For this device, commonly available solar control glass plates can be used. The design of this filter is based on the fact that both visible and UV radiation comply with the law of Reflection and Refraction of light. As light rays appeared on the glass plate, most of the rays pass through the glass after refraction. A portion of the incident ray is mirrored and goes out to the next glass panel, where the same thing is going to happen. Through this way, multiple glass plates reflect the light ray before diffuse light is received. Owing to the fact that diffuse reflection is responsible for the ability to see most illuminated objects, we will be able to see the dull image of sun directly through this filter during the solar eclipse.
Recommended
Recent Advances in Integrated Pest Management of Sorghum
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of
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Etude de la Phyllosphère
Présentation du projet de recherche réalisé au Biodôme de Montréal avec le soutien de l'Université de Clermont-Ferrand et l'IUT des Cezeaux.
Sclerotia production a way ahead to morchella Cultivation
Morchella, the true morels, belonging to Helvellaceae family of class
Ascomycetes, are amongst the most highly priced fungi in the world. Their
artificial production is still a challenge, even though patents for their
cultivation do exist. The tissue of Morchella sp. was transferred aseptically
to Potato Dextrose Agar medium (peeled, sliced and boiled potato, 200 g;
dextrose, 20 g; agar, 20 g L-1) to grow hyphae. The mycelium showed
fastest growth as compared to other edible mushrooms. It covered entire area
of Petri plate (90 mm) within 4-5 days with the growth rate of 18-
22.5mm/day. A unique growth pattern i.e., vertically oriented mycelia were
observed. Brown coloured pigmentation in the culture was also observed
during the study. The basal media for spawn (wheat grains; Glucose, 1%,
CaCO3, 2%; CaSO4, 1.5% and MgSO4, 1%) was aseptically inoculated with
the mushroom culture. The spawn substrate was colonized by mushroom
mycelium in 7-8 days. The sclerotia were formed in unused (old) spawn.
Wheat straw was used as a substrate for mushroom cultivation. It was
supplemented with wheat bran, 20%, Glucose (1%) and MgSO4 (1%). It
showed prolific growth when it was seeded by mushroom spawn using jar
method. Once substrate was fully covered with mushroom mycelium, casing
was applied. Sclerotia were successfully obtained after 14 days of incubation
in our experiment both in the substrate and casing soil but failed to give rise
to fruiting primordial. Further research is going on to domesticate this
species in our country.
Sclerotia production a way ahead to morchella cultivation
Morchella, the true morels, belonging to Helvellaceae family of class Ascomycetes, are amongst the most highly prized fungi in the world. Their artificial production is still a challenge, even though patents for their cultivation do exist. The tissue of Morchella sp. was transferred aseptically to Potato Dextrose Agar medium (peeled, sliced and boiled potato, 200 g; dextrose, 20 g; agar, 20 g L-1) to grow hyphae. The mycelium showed fastest growth as compared to other edible mushrooms. It covered entire area of Petri plate (90 mm) within 4-5 days with the growth rate of 18-22.5mm/day. A unique growth pattern i.e. vertically oriented mycelia were
observed. Brown coloured pigmentation in the culture was also observed during the study. The basal media for spawn (wheat grains; Glucose, 1%, CaCO3, 2%; CaSO4, 1.5% and MgSO4, 1%) was aseptically inoculated with the mushroom culture. The spawn substrate was colonized by mushroom mycelium in 7-8 days. The sclerotia were formed in unused (old) spawn. Wheat
straw was used as a substrate for mushroom cultivation. It was supplemented with wheat bran, 20%, Glucose (1%) and MgSO4 (1%). It showed prolific growth when it was seeded by
mushroom spawn using jar method. Once substrate was fully covered with mushroom mycelium, casing was applied. Sclerotia were successfully obtained after 14 days of incubation in our experiment both in the substrate and casing soil but failed to give rise to fruiting primordial. Further research is going on to domesticate this species in this part of country.
A new filter for safe view of solar eclipse
Looking directly at the sun can lead to permanent eye injury due to damage of light-sensitive rod and cone cells within the retina. There are two ways to look at the Sun safely: by observing the sun directly through a suitable filter, or by projecting the Sun’s reflection onto a piece of paper via handmade pinhole camera/ telescope. The present correspondence shows the specification for the development of an optical instrument for the direct observation of sun. For this device, commonly available solar control glass plates can be used. The design of this filter is based on the fact that both visible and UV radiation comply with the law of Reflection and Refraction of light. As light rays appeared on the glass plate, most of the rays pass through the glass after refraction. A portion of the incident ray is mirrored and goes out to the next glass panel, where the same thing is going to happen. Through this way, multiple glass plates reflect the light ray before diffuse light is received. Owing to the fact that diffuse reflection is responsible for the ability to see most illuminated objects, we will be able to see the dull image of sun directly through this filter during the solar eclipse.
An Approach to Improve Yield Parameters of Pleurotus florida Strain P1
Aim: The efficient mushroom production involves many factors of which spawn production, culture methods and substrate selection are of prime importance. The research has, therefore, been carried out to standardize these aspects in present communication, for production of Pleurotus florida Strain P1.
Materials and Methods: Various cereals (maize, oat, barley) and millets (Italian millets, Little millet, Pearl millet) were assessed against wheat grain spawn for enhancing yield and biological efficiency of mushroom. Most suitable wheat straw component among fine pieces of leaves and leaf sheath (0.2
cm), coarse pieces of leaves and leaf sheath (0.4 cm), small (1.0 cm) and large pieces of the stem (1.7 cm) were also evaluated for the manifestations above. Various culture methods viz., bag, column, wall and tray culture and few substrate mixtures (corncob + wheat straw, mango sawdust + wheat straw and rice husk + wheat straw) have also been taken to increase yield performance of mushroom.
Results: The result showed that barley and oat grains produced good quality spawn in terms of quick mycelial running on the grain surface (14 days each). These spawn produced acceleration of spawn running and increased yield, as compared to other types of spawn. When different components of wheat straw were evaluated for the yield performance of mushroom, the course pieces of the stem (1.0 cm) proved the most appropriate component in relation to very low/zero contamination along highest yield (819 gm) and biological efficiency (163.8%). In contrast, wheat straw and corn cob were
recognized as the best substrate combination with highest yield (707 gm) and biological efficiency (141.4%) of mushrooms. Among different culture techniques used, only the column method gave significant yield (930 gm) and biological efficiency (186%) with appropriate mushrooms sizes from
packaging point of view.
Conclusion: The results obtained during the study revealed that by adopting the composite approach, the growers enhance mushroom production in manyfolds. The mushroom is cultivated by following column method. The wheat straw devoid from pieces of leaf & leaf sheath should be utilized
as a substrate with corncob combination. It should be inoculated with oat/barley spawn to higher yield.
A new filter for safe view of Solar Eclipse
Looking directly at the sun can lead to permanent eye injury due to damage of light-sensitive rod and cone cells within the retina. There are two ways to look at the Sun safely: by observing the sun directly through a suitable filter, or by projecting the Sun’s reflection onto a piece of paper via handmade pinhole camera/ telescope. The present correspondence shows the specification for the development of an optical instrument for the direct observation of sun. For this device, commonly available solar control glass plates can be used. The design of this filter is based on the fact that both visible and UV radiation comply with the law of Reflection and Refraction of light. As light rays appeared on the glass plate, most of the rays pass through the glass after refraction. A portion of the incident ray is mirrored and goes out to the next glass panel, where the same thing is going to happen. Through this way, multiple glass plates reflect the light ray before diffuse light is received. Owing to the fact that diffuse reflection is responsible for the ability to see most illuminated objects, we will be able to see the dull image of sun directly through this filter during the solar eclipse.
A new filter for safe view of Solar Eclipse
Looking directly at the sun can lead to permanent eye injury due to damage of light-sensitive rod and cone cells within the retina. There are two ways to look at the Sun safely: by observing the sun directly through a suitable filter, or by projecting the Sun’s reflection onto a piece of paper via handmade pinhole camera/ telescope. The present correspondence shows the specification for the development of an optical instrument for the direct observation of sun. For this device, commonly available solar control glass plates can be used. The design of this filter is based on the fact that both visible and UV radiation comply with the law of Reflection and Refraction of light. As light rays appeared on the glass plate, most of the rays pass through the glass after refraction. A portion of the incident ray is mirrored and goes out to the next glass panel, where the same thing is going to happen. Through this way, multiple glass plates reflect the light ray before diffuse light is received. Owing to the fact that diffuse reflection is responsible for the ability to see most illuminated objects, we will be able to see the dull image of sun directly through this filter during the solar eclipse.
Bottle culture: A suitable method for oyster mushroom cultivation
C.S. Singh, Siddhant, Ruchira Singh and R.S. Kanaujia (2006): Bottle culture: A suitable method for oyster mushroom cultivation. Environmental Biology and Conservation . 11:25-26
Comparison of Growth and Development of Pleurotus florida against Wastes from...
Two wastes from animal origin, viz., human hairs and egg shells were evaluated for different manifestations of white oyster mushroom, Pleurotus florida. The mushroom utilized both the
substrates for their growth and sporophore formation. The mycelial growth was significantly (P=0.05) faster on egg shell (18 days) as compared to human hair (23 days). The crop of mushroom was harvested in four flushes where human hairs showed higher yield and biological efficiency of mushroom (165 gm, 33%) than egg shells (155 gm, 31%), respectively. In respect of yield parameters such as yield, biological efficiency, number of mushroom fruit bodies and average weight of
sporophores, both the substrates were statistically at par to each other. Utilization of human hairs egg shells by P. florida reveals a new strategy for mycoremediation of these wastes.
Determination of the Paper Quality as a Substrate for Oyster Mushroom Cultiva...
Eight different kinds of papers, viz., glaze paper, brown paper, news paper, magazine paper, chart paper, kite paper, rough copy paper and A-4 size printing paper, and two types of cardboard viz., corrugated cardboard and card board were evaluated for different manifestations of white oyster
mushroom Pleurotus florida Strain-P1. Among them news paper was later treated as a control. The mushroom was utilized all the substrates for their growth and sporophore formation. Majority substrates were taken almost equal time for spawn run, primordial development and fruiting bodies
maturation. The measured parameters were net yield (Weight of fresh mushrooms), biological efficiency, number of fruiting bodies produced and average weight of sporophores varied among themselves. The crop of mushroom was harvested in three flushes where yield and biological efficiency ranged 190-495 gm, 38-99% for the substrate used. Magazine paper (450 gm; 90%) and card board (495 gm; 99%) produced significant (P=0.05) yield and biological efficiency over control.
They also produced significant number of mushroom fruit bodies (56 and 64, respectively). Corrugated cardboard (10.29 gm) was found significant in terms of average weight per sporocarp. The percentage yield of different substrates was also evaluated. Among the substrates, card board
contributed 14% of total mushroom production followed by magazine paper (13%) and news paper (12%).
Transform papers-into-food
Eight different kinds of papers, viz., glaze paper, brown paper, news paper, magazine paper, chart paper, kite paper, rough copy paper and A-4 size printing paper and two types of cardboards viz., corrugated cardboard and card board were evaluated for different manifestations of white oyster mushroom Pleurotus florida Strain-P1. Among them news paper was later treated as a control. The mushroom utilized all the substrates for their growth and sporophore formation. Majority of substrates took almost equal time for spawn run primordial development and fruit bodies maturation. The yield parameters such as yield, biological efficiency, number of mushroom fruit bodies and average weight of sporophores varied among themselves. The crop of mushroom was harvested in three flushes where yield and biological efficiency ranged 190-495 gm, 38-99% for the substrate used. Magazine paper (450 gm; 90%) and card board (495 gm; 99%) produced significant (P=0.05) yield and biological efficiency over control. They also produced significant number of mushroom fruit bodies (56 and 64, respectively). Corrugated cardboard (10.29 gm) was found significant in terms of average weight per sporocarp. The percentage yield of different substrates was also evaluated. Among the substrates, card board contributed 14 % of total mushroom production followed by magazine paper (13%) and news paper (12%).
A Modified Approach in Substrate Preparation Technique for Small Scale Oyster...
Aims: Cultivation of oyster mushrooms has increased vastly in a global scale during last few
decades. Contaminants and indigenous microflora present in the substrate may led to the low
productivity of mushrooms. Keeping this in mind, the present study was under-taken with slight
modification in substrate preparation technique to eliminate dust particles from the substrate and to
assure contamination free mushroom production.
Study Design: Comparative evaluation between modified approach and control beds.
Place and Duration: The study was carried out in Shri Laxman Prasad Pyare Lal Agro Products,
Ayodhya during 2017.
Methodology: The wheat straw substrate was immersed in the drum containing tap water, mixing
properly and allowed to stand for 10-15 min to settle down the dust particle in the bottom of the
drum. After that, the floating substrate was transferred to the slant surface so that the extra water
was decanted off. This substrate was put in to the steam sterilised gunny bag and steeped in the
chemical solution consisting of Formaldehyde (500 ppm) and Bovistin (75 ppm) for 18 h. For the
Short Communication
Siddhant et al.; AJAHR, 2(2): 1-5, 2018; Article no.AJAHR.44907
2
control, fresh wheat straw was put in to the steam sterilised gunny bag and steeped directly in the
chemical solution as suggested above. After removal of excess water, the substrate was used for
spawning with inoculums of Pleurotus sajor-caju, Strain- Malaysia.
Results: Beds with modified approach showed a complete absence of contaminants during entire
crop cycle which showed the effectiveness of modified method while beds treated as control
showed little incidence of various contaminants viz., Alternaria alternata, Aspergillus fumigatus,
Curvularia sp., Penicillium sp., and Rhizopus stolonifer with 6.67-20.00 percent incidence.
Conclusion: The results revealed that the modified method should be considered to contamination
free mushroom production.
Comparison of Growth and Development of Pleurotus florida against Wastes fr...
Two wastes from animal origin, viz., human hairs and egg shells were evaluated for different manifestations of white oyster mushroom, Pleurotus florida. The mushroom utilized both the substrates for their growth and sporophore formation. The mycelial growth was significantly (P=0.05) faster on egg shell (18 days) as compared to human hair (23 days). The crop of mushroom was harvested in four flushes where human hairs showed higher yield and biological efficiency of mushroom (165gm, 33%) than egg shells (155gm, 31%), respectively. In respect of yield parameters such as yield, biological efficiency, number of mushroom fruit bodies and average weight of sporophores, both the substrates were statistically at par to each other. Utilization of human hairs and egg shells by P. florida reveals a new strategy for mycoremediation of these wastes.
Spawned Casing vs Simple Casing
Effect of spawned and simple casing on the biological efficiency of pink oyster mushroom Pleurotus eous was observed. The results exhibit that spawned casing not only takes lesser time for case run but also produces early primordial development. A positive response on mushroom biomass is also noticed in this technique.
A demonstration of mushroom cultivation on bracket fungi
Bracket fungi were used probably first time as a growing medium in the cultivation history of Pleurotus sajor-caju.
Wet rot disease of Cereus hexagonus and fruit rot of Syzygium cumini, new to ...
During an extensive survey of fungi from Faizabad (U.P.), the author came across two important diseases, viz., wet rot of Cereus hexagonus caused by Periconia byssoides Pers ex Me'rat and fruit rot of Syzygium cumini (L.) Skeels caused by Rhizopus nigricans Ehrenb. ex Corda, new to India. The specimen has been deposited in the Department of Botany, K.S.S. P.G. College, Faizabad under Accession No. RSK 415 and 558.
Record of periconia byssoides on four o clock plant from india
In September, 1978, the author observed plants of Mirabilis jalapa L. infected by wet disease at Faizabad. On examination the pathogen responsible for the disease was found to be Periconia byssoides Pers. ex Me'rat. The specimen has been deposited in the Department of Botany, K.S.S. P.G. College, Faizabad as Herbarium No. RSK-260. The present case is a new host record for India.
Periconia rot of capsicum annum in india
Periconia byssoides is known to parasite a number of plants in India but so far not reported on Capsicum annuum L. The small spherical to irregular spots of light brown colour gradually enlarged and merged subsequently to form continuous dark brown to black patches on stem, leaves and fruits. The whole plant were soon infected and ultimately dried within 20-25 days. The damage was about 70-85 per cent. The fungus was isolated on PDA medium by usual methods. The specimen has been deposited (No. RSK-142) in the herbarium of Botany Department, K.S.S. P.G. College, Faizabad.
Cited this as: R.S. Kanaujia and R.S. Verma (1979): Periconia rot of Capsicum annuum in India. Indian Phytopathology. 32(3): 445.
Fruit rot of mandarin orange
In December 1978, the author observed the stored fruits of Citrus reticulata Blanco severely infected by a fungus causing soft rot in local fruit market of Faizabad. The entire fruit surface was densely covered by the fungus causing the rot. The fungus was isolated on PDA medium and identified as Trichoderma lignorum (Tode) harz.
In India, T. lignorum has been found on various substrates, viz., soil, rhizosphere region of Pennisetum typhoides, paddy straw of mushroom beds, phyllosphere of Impatiens balsamina and Citrus crysocarpa but not on Citrus reticulata and the present association is a new record for India.
Cited this as: R.S. Kanaujia (1979): Fruit rot of mandarin orange. Indian Phytopathology. 32(3):450.
Notes on a new fungal disease of alocasia indica
Short hole disease of Alocasia indica Schott, caused by Phoma exigua Desm., earlier unknown to India has been reported from Faizabad. The infection was chiefly confined to the margins of lamina. It started as small nonchlorophyllous spots near the fronds. The spots gradually enlarged irregularly upto 2-3 cm sq. area of the leaf, merged and in final stage, necrosis and then short holes appeared within a month. The pathogenicity tests performed by standard methods proved pathogenic nature of the fungus.
Wet rot of amaranthus viridis
During survey of fungi causing disease in plants at Faizabad (U.P.), authors observed infection of wet rot disease on Amaranthus viridis L. plants growing as mixed crop with Abelmoschus esculentus at Ram Kote, Ayodhya of Faizabad district in September, 1976. The infection was severe during flowering when percentage of infection recorded was upto 60-70. The microscopic studies of the original specimen and that grown on Czapek's Agar Medium match with the description of Gilman for Choanephora cucurbitarum (Berk. & Rav.) Thaxter. The pathogenicity of the fungus was established. This is the first report of this fungus on Amaranthus viridis (Chaulai).
Abnormality in archegoniophore of marchantia polymorpha l.
Abnormality in the reproductive organs of different genera in order Marchantiales has been described by various workers. The specimen of Marchantia polymorpha L., collected from Mani parvat at Ayodhya in Faizabad exhibits the emergence of an abnormal vegetative branch with gemma cup from the upper half of the archegoniophore. This has been described for the first time from U.P. plains.
Effect of cereal, millet and legume bran supplement on yield and biological e...
Different bran, viz., wheat, rice, maize, barley (cereal bran), sorghum (millet bran) pea, gram and pigeon pea (legume bran) were evaluated as supplement @ 10% on dry weight basis of substrate for enhancing yield and biological efficiency of Pleurotus flabellatus. Among the bran used, wheat, rice, sorghum, pea and pigeon pea bran produced significant yield and biological efficiency of mushroom than the control. It was recorded maximum in rice bran (655 g, 131%) followed by wheat bran (615 g, 123%), pea bran (600 g, 120%), pigeon pea bran (595 g, 109%) and sorghum bran (580 g, 116%). Significantly (P=0.05) higher weight/sporocarp was obtained from the barley (12.50 g), pea (13.63 g) and pigeon pea bran (15.42 g) while none of them produced significant no of sporocarp.
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An Approach to Improve Yield Parameters of Pleurotus florida Strain P1
Aim: The efficient mushroom production involves many factors of which spawn production, culture methods and substrate selection are of prime importance. The research has, therefore, been carried out to standardize these aspects in present communication, for production of Pleurotus florida Strain P1.
Materials and Methods: Various cereals (maize, oat, barley) and millets (Italian millets, Little millet, Pearl millet) were assessed against wheat grain spawn for enhancing yield and biological efficiency of mushroom. Most suitable wheat straw component among fine pieces of leaves and leaf sheath (0.2
cm), coarse pieces of leaves and leaf sheath (0.4 cm), small (1.0 cm) and large pieces of the stem (1.7 cm) were also evaluated for the manifestations above. Various culture methods viz., bag, column, wall and tray culture and few substrate mixtures (corncob + wheat straw, mango sawdust + wheat straw and rice husk + wheat straw) have also been taken to increase yield performance of mushroom.
Results: The result showed that barley and oat grains produced good quality spawn in terms of quick mycelial running on the grain surface (14 days each). These spawn produced acceleration of spawn running and increased yield, as compared to other types of spawn. When different components of wheat straw were evaluated for the yield performance of mushroom, the course pieces of the stem (1.0 cm) proved the most appropriate component in relation to very low/zero contamination along highest yield (819 gm) and biological efficiency (163.8%). In contrast, wheat straw and corn cob were
recognized as the best substrate combination with highest yield (707 gm) and biological efficiency (141.4%) of mushrooms. Among different culture techniques used, only the column method gave significant yield (930 gm) and biological efficiency (186%) with appropriate mushrooms sizes from
packaging point of view.
Conclusion: The results obtained during the study revealed that by adopting the composite approach, the growers enhance mushroom production in manyfolds. The mushroom is cultivated by following column method. The wheat straw devoid from pieces of leaf & leaf sheath should be utilized
as a substrate with corncob combination. It should be inoculated with oat/barley spawn to higher yield.
A new filter for safe view of Solar Eclipse
Looking directly at the sun can lead to permanent eye injury due to damage of light-sensitive rod and cone cells within the retina. There are two ways to look at the Sun safely: by observing the sun directly through a suitable filter, or by projecting the Sun’s reflection onto a piece of paper via handmade pinhole camera/ telescope. The present correspondence shows the specification for the development of an optical instrument for the direct observation of sun. For this device, commonly available solar control glass plates can be used. The design of this filter is based on the fact that both visible and UV radiation comply with the law of Reflection and Refraction of light. As light rays appeared on the glass plate, most of the rays pass through the glass after refraction. A portion of the incident ray is mirrored and goes out to the next glass panel, where the same thing is going to happen. Through this way, multiple glass plates reflect the light ray before diffuse light is received. Owing to the fact that diffuse reflection is responsible for the ability to see most illuminated objects, we will be able to see the dull image of sun directly through this filter during the solar eclipse.
A new filter for safe view of Solar Eclipse
Looking directly at the sun can lead to permanent eye injury due to damage of light-sensitive rod and cone cells within the retina. There are two ways to look at the Sun safely: by observing the sun directly through a suitable filter, or by projecting the Sun’s reflection onto a piece of paper via handmade pinhole camera/ telescope. The present correspondence shows the specification for the development of an optical instrument for the direct observation of sun. For this device, commonly available solar control glass plates can be used. The design of this filter is based on the fact that both visible and UV radiation comply with the law of Reflection and Refraction of light. As light rays appeared on the glass plate, most of the rays pass through the glass after refraction. A portion of the incident ray is mirrored and goes out to the next glass panel, where the same thing is going to happen. Through this way, multiple glass plates reflect the light ray before diffuse light is received. Owing to the fact that diffuse reflection is responsible for the ability to see most illuminated objects, we will be able to see the dull image of sun directly through this filter during the solar eclipse.
Bottle culture: A suitable method for oyster mushroom cultivation
C.S. Singh, Siddhant, Ruchira Singh and R.S. Kanaujia (2006): Bottle culture: A suitable method for oyster mushroom cultivation. Environmental Biology and Conservation . 11:25-26
Comparison of Growth and Development of Pleurotus florida against Wastes from...
Two wastes from animal origin, viz., human hairs and egg shells were evaluated for different manifestations of white oyster mushroom, Pleurotus florida. The mushroom utilized both the
substrates for their growth and sporophore formation. The mycelial growth was significantly (P=0.05) faster on egg shell (18 days) as compared to human hair (23 days). The crop of mushroom was harvested in four flushes where human hairs showed higher yield and biological efficiency of mushroom (165 gm, 33%) than egg shells (155 gm, 31%), respectively. In respect of yield parameters such as yield, biological efficiency, number of mushroom fruit bodies and average weight of
sporophores, both the substrates were statistically at par to each other. Utilization of human hairs egg shells by P. florida reveals a new strategy for mycoremediation of these wastes.
Determination of the Paper Quality as a Substrate for Oyster Mushroom Cultiva...
Eight different kinds of papers, viz., glaze paper, brown paper, news paper, magazine paper, chart paper, kite paper, rough copy paper and A-4 size printing paper, and two types of cardboard viz., corrugated cardboard and card board were evaluated for different manifestations of white oyster
mushroom Pleurotus florida Strain-P1. Among them news paper was later treated as a control. The mushroom was utilized all the substrates for their growth and sporophore formation. Majority substrates were taken almost equal time for spawn run, primordial development and fruiting bodies
maturation. The measured parameters were net yield (Weight of fresh mushrooms), biological efficiency, number of fruiting bodies produced and average weight of sporophores varied among themselves. The crop of mushroom was harvested in three flushes where yield and biological efficiency ranged 190-495 gm, 38-99% for the substrate used. Magazine paper (450 gm; 90%) and card board (495 gm; 99%) produced significant (P=0.05) yield and biological efficiency over control.
They also produced significant number of mushroom fruit bodies (56 and 64, respectively). Corrugated cardboard (10.29 gm) was found significant in terms of average weight per sporocarp. The percentage yield of different substrates was also evaluated. Among the substrates, card board
contributed 14% of total mushroom production followed by magazine paper (13%) and news paper (12%).
Transform papers-into-food
Eight different kinds of papers, viz., glaze paper, brown paper, news paper, magazine paper, chart paper, kite paper, rough copy paper and A-4 size printing paper and two types of cardboards viz., corrugated cardboard and card board were evaluated for different manifestations of white oyster mushroom Pleurotus florida Strain-P1. Among them news paper was later treated as a control. The mushroom utilized all the substrates for their growth and sporophore formation. Majority of substrates took almost equal time for spawn run primordial development and fruit bodies maturation. The yield parameters such as yield, biological efficiency, number of mushroom fruit bodies and average weight of sporophores varied among themselves. The crop of mushroom was harvested in three flushes where yield and biological efficiency ranged 190-495 gm, 38-99% for the substrate used. Magazine paper (450 gm; 90%) and card board (495 gm; 99%) produced significant (P=0.05) yield and biological efficiency over control. They also produced significant number of mushroom fruit bodies (56 and 64, respectively). Corrugated cardboard (10.29 gm) was found significant in terms of average weight per sporocarp. The percentage yield of different substrates was also evaluated. Among the substrates, card board contributed 14 % of total mushroom production followed by magazine paper (13%) and news paper (12%).
A Modified Approach in Substrate Preparation Technique for Small Scale Oyster...
Aims: Cultivation of oyster mushrooms has increased vastly in a global scale during last few
decades. Contaminants and indigenous microflora present in the substrate may led to the low
productivity of mushrooms. Keeping this in mind, the present study was under-taken with slight
modification in substrate preparation technique to eliminate dust particles from the substrate and to
assure contamination free mushroom production.
Study Design: Comparative evaluation between modified approach and control beds.
Place and Duration: The study was carried out in Shri Laxman Prasad Pyare Lal Agro Products,
Ayodhya during 2017.
Methodology: The wheat straw substrate was immersed in the drum containing tap water, mixing
properly and allowed to stand for 10-15 min to settle down the dust particle in the bottom of the
drum. After that, the floating substrate was transferred to the slant surface so that the extra water
was decanted off. This substrate was put in to the steam sterilised gunny bag and steeped in the
chemical solution consisting of Formaldehyde (500 ppm) and Bovistin (75 ppm) for 18 h. For the
Short Communication
Siddhant et al.; AJAHR, 2(2): 1-5, 2018; Article no.AJAHR.44907
2
control, fresh wheat straw was put in to the steam sterilised gunny bag and steeped directly in the
chemical solution as suggested above. After removal of excess water, the substrate was used for
spawning with inoculums of Pleurotus sajor-caju, Strain- Malaysia.
Results: Beds with modified approach showed a complete absence of contaminants during entire
crop cycle which showed the effectiveness of modified method while beds treated as control
showed little incidence of various contaminants viz., Alternaria alternata, Aspergillus fumigatus,
Curvularia sp., Penicillium sp., and Rhizopus stolonifer with 6.67-20.00 percent incidence.
Conclusion: The results revealed that the modified method should be considered to contamination
free mushroom production.
Comparison of Growth and Development of Pleurotus florida against Wastes fr...
Two wastes from animal origin, viz., human hairs and egg shells were evaluated for different manifestations of white oyster mushroom, Pleurotus florida. The mushroom utilized both the substrates for their growth and sporophore formation. The mycelial growth was significantly (P=0.05) faster on egg shell (18 days) as compared to human hair (23 days). The crop of mushroom was harvested in four flushes where human hairs showed higher yield and biological efficiency of mushroom (165gm, 33%) than egg shells (155gm, 31%), respectively. In respect of yield parameters such as yield, biological efficiency, number of mushroom fruit bodies and average weight of sporophores, both the substrates were statistically at par to each other. Utilization of human hairs and egg shells by P. florida reveals a new strategy for mycoremediation of these wastes.
Spawned Casing vs Simple Casing
Effect of spawned and simple casing on the biological efficiency of pink oyster mushroom Pleurotus eous was observed. The results exhibit that spawned casing not only takes lesser time for case run but also produces early primordial development. A positive response on mushroom biomass is also noticed in this technique.
A demonstration of mushroom cultivation on bracket fungi
Bracket fungi were used probably first time as a growing medium in the cultivation history of Pleurotus sajor-caju.
Wet rot disease of Cereus hexagonus and fruit rot of Syzygium cumini, new to ...
During an extensive survey of fungi from Faizabad (U.P.), the author came across two important diseases, viz., wet rot of Cereus hexagonus caused by Periconia byssoides Pers ex Me'rat and fruit rot of Syzygium cumini (L.) Skeels caused by Rhizopus nigricans Ehrenb. ex Corda, new to India. The specimen has been deposited in the Department of Botany, K.S.S. P.G. College, Faizabad under Accession No. RSK 415 and 558.
Record of periconia byssoides on four o clock plant from india
In September, 1978, the author observed plants of Mirabilis jalapa L. infected by wet disease at Faizabad. On examination the pathogen responsible for the disease was found to be Periconia byssoides Pers. ex Me'rat. The specimen has been deposited in the Department of Botany, K.S.S. P.G. College, Faizabad as Herbarium No. RSK-260. The present case is a new host record for India.
Periconia rot of capsicum annum in india
Periconia byssoides is known to parasite a number of plants in India but so far not reported on Capsicum annuum L. The small spherical to irregular spots of light brown colour gradually enlarged and merged subsequently to form continuous dark brown to black patches on stem, leaves and fruits. The whole plant were soon infected and ultimately dried within 20-25 days. The damage was about 70-85 per cent. The fungus was isolated on PDA medium by usual methods. The specimen has been deposited (No. RSK-142) in the herbarium of Botany Department, K.S.S. P.G. College, Faizabad.
Cited this as: R.S. Kanaujia and R.S. Verma (1979): Periconia rot of Capsicum annuum in India. Indian Phytopathology. 32(3): 445.
Fruit rot of mandarin orange
In December 1978, the author observed the stored fruits of Citrus reticulata Blanco severely infected by a fungus causing soft rot in local fruit market of Faizabad. The entire fruit surface was densely covered by the fungus causing the rot. The fungus was isolated on PDA medium and identified as Trichoderma lignorum (Tode) harz.
In India, T. lignorum has been found on various substrates, viz., soil, rhizosphere region of Pennisetum typhoides, paddy straw of mushroom beds, phyllosphere of Impatiens balsamina and Citrus crysocarpa but not on Citrus reticulata and the present association is a new record for India.
Cited this as: R.S. Kanaujia (1979): Fruit rot of mandarin orange. Indian Phytopathology. 32(3):450.
Notes on a new fungal disease of alocasia indica
Short hole disease of Alocasia indica Schott, caused by Phoma exigua Desm., earlier unknown to India has been reported from Faizabad. The infection was chiefly confined to the margins of lamina. It started as small nonchlorophyllous spots near the fronds. The spots gradually enlarged irregularly upto 2-3 cm sq. area of the leaf, merged and in final stage, necrosis and then short holes appeared within a month. The pathogenicity tests performed by standard methods proved pathogenic nature of the fungus.
Wet rot of amaranthus viridis
During survey of fungi causing disease in plants at Faizabad (U.P.), authors observed infection of wet rot disease on Amaranthus viridis L. plants growing as mixed crop with Abelmoschus esculentus at Ram Kote, Ayodhya of Faizabad district in September, 1976. The infection was severe during flowering when percentage of infection recorded was upto 60-70. The microscopic studies of the original specimen and that grown on Czapek's Agar Medium match with the description of Gilman for Choanephora cucurbitarum (Berk. & Rav.) Thaxter. The pathogenicity of the fungus was established. This is the first report of this fungus on Amaranthus viridis (Chaulai).
Abnormality in archegoniophore of marchantia polymorpha l.
Abnormality in the reproductive organs of different genera in order Marchantiales has been described by various workers. The specimen of Marchantia polymorpha L., collected from Mani parvat at Ayodhya in Faizabad exhibits the emergence of an abnormal vegetative branch with gemma cup from the upper half of the archegoniophore. This has been described for the first time from U.P. plains.
Effect of cereal, millet and legume bran supplement on yield and biological e...
Different bran, viz., wheat, rice, maize, barley (cereal bran), sorghum (millet bran) pea, gram and pigeon pea (legume bran) were evaluated as supplement @ 10% on dry weight basis of substrate for enhancing yield and biological efficiency of Pleurotus flabellatus. Among the bran used, wheat, rice, sorghum, pea and pigeon pea bran produced significant yield and biological efficiency of mushroom than the control. It was recorded maximum in rice bran (655 g, 131%) followed by wheat bran (615 g, 123%), pea bran (600 g, 120%), pigeon pea bran (595 g, 109%) and sorghum bran (580 g, 116%). Significantly (P=0.05) higher weight/sporocarp was obtained from the barley (12.50 g), pea (13.63 g) and pigeon pea bran (15.42 g) while none of them produced significant no of sporocarp.
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An Approach to Improve Yield Parameters of Pleurotus florida Strain P1
An Approach to Improve Yield Parameters of Pleurotus florida Strain P1
Bottle culture: A suitable method for oyster mushroom cultivation
Bottle culture: A suitable method for oyster mushroom cultivation
Comparison of Growth and Development of Pleurotus florida against Wastes from...
Comparison of Growth and Development of Pleurotus florida against Wastes from...
Determination of the Paper Quality as a Substrate for Oyster Mushroom Cultiva...
Determination of the Paper Quality as a Substrate for Oyster Mushroom Cultiva...
A Modified Approach in Substrate Preparation Technique for Small Scale Oyster...
A Modified Approach in Substrate Preparation Technique for Small Scale Oyster...
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Comparison of Growth and Development of Pleurotus florida against Wastes fr...
A demonstration of mushroom cultivation on bracket fungi
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Wet rot disease of Cereus hexagonus and fruit rot of Syzygium cumini, new to ...
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Record of periconia byssoides on four o clock plant from india
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The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
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Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
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Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
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Slide 5: Plasmid Inheritance
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Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
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Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
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