The science behind optogentics and its potential applications in neuroscience. An 8-minute presentation for an audience of fellow neural engineering students.

1. OptogeneticsShedding Light on Optogenetics
Ray Ward

2. Historical Methods of Neural Manipulation
● Lesioning
● Genetic Modification
● Physical Stimulation
● Electrical Stimulation
● Drugs
[2] H-Y. Lai et al., 2012
Specificity?
Invasiveness?
Duration? Speed?

3. Introducing Optogenetics
Cells genetically modified to respond to light
Specificity?
Invasiveness?
Duration? Speed?
[3] S. B. Goncalves
[4] Deisseroth, 2010

4. How it Works
[1] Buchen, 2010

5. Introducing Optogenetics
Cells genetically modified to respond to light
Specificity?
Invasiveness?
Duration? Speed?
[3] Buchen, 2017
[4] Deisseroth, 2010

6. Optogenetics in Neuroscience
Images and Video by Karl Deisseroth
[1] Buchen, 2010

7. Applications of Neurogenetics
Epidural Optogenetics for
Analgesia
Genetic dissection of an amygdala
microcircuit that gates conditioned fear
[5] W. Haubensak
[6] R. Bonin, 2016

8. Bibliography
[1] L. Buchen “Neuroscience: Illuminating the Brain” Nature vol. 465, 26-28, MAY 5, 2010
[2] H-Y. Lai, L-D Liao, C-T. Lin, et al. “Design, simulation and experimental validation of a novel flexible neural probe for deep brain stimulation and
multichannel recording.“ Journal of Neural Engineering, vol. 9, APR 10, 2012
[3] S. B. Goncalves, J. F. Ribeiro, A. F. Silva, et al. “Design and Manufacturing Challenges of Optogenetics Neural Interfaces: A Review” Journal of Neural
Engineering, vol. 14.4, MAY 19, 2017
[4] K. Deisseroth “Optogenetics” Nature: Methods, vol. 8, 26-29, DEC 20, 2010
[5] W. Haubensak, P. Kunwar, H. Cai, et al. “Genetic dissection of an amygdala microcircuit that gates conditioned fear” Nature vol. 468, 270-276, NOV 11,
2010
[6] R. Bonin, F. Wang, M. Desrochers-Couture, et al. “Epidural optogenetics for controlled analgesia” Molecular Pain vol. 12, 1-11, MAR 9, 2016