seminario biología molecular, Laura Sofia Jiménez, tercer semestre, Universidad Pontificia Bolivariana

1. Laura Sofia Jimenez
Third semester
Universidad Pontificia Bolivariana

2. OVARIAN CANCER
• Uncontrolled growth of abnormal cells in the body
that form tumors in the tissue of the ovaries
• There are differents types. The most common ins
epitelial cancer.
• Fallopian tuve cancer and primary peritoneal cancer
can spread to the ovaries.
• Most ovarian cancer are caused by BRCA1 and
BRCA2 gene changes, those also increase the risk of
breast cancer.

3. ANOIKIS
Is a programmed cell death
induced by the detachment of cells
from extracelular matrix.
Normal cells undergo apoptosis as
a mechanism against metastasis.

4. GENERAL OBJECTIVE
“we aimed to assess
wheather visfatin which
possess antiapoptotic
properties can induce
resistance of anoikis in
ovarian cancer
spheroids”.

5. METHODOLOGY
the accumulation of amplified DNA is
detected and quantified as the
reaction progresses (in real time). This
is achieved by incorporating a
fluorescent molecule that associates
with the amplified DNA, where the
increase in this fluorescence is
proportional to the increase in the
amount of amplified DNA molecules in
the reaction
PCR REAL TIME WESTERN BLOT ANALYSIS
is a technique used to study a
specific protein in a sample that
contains many other proteins. The
protein of interest is identified using
a radiolabeled or enzyme-linked
antibody specific for the protein.

6. CELL VIABILITY ASSAY
This test is based on colorimetric,
fluorometric and bioluminescent
detection methods that allow
determination of enzymatic
activity, cell membrane integrity
and ATP production
VISFATIN CELL SYNTHESIS
ANÁLISIS (ELISA)
This test is classified as a
immunoassay that evaluates
the reaction between
antigen and antibody, it
marks the reaction as an
enzyme.

7. RESULTS
Visfatin and Caspase 3
• Decreased level of caspase 3 activity.
• Visfatin treatment did not have an effect
on PARP1 DNA expresión or PARP1
protein cleavage
Visfatin and Caspase 8 y 9 in the expresión of
mRNA and proteins Bax and Bcl-2 and the
activation of BID
• Decrease in the expresión of mRNA and the Bax
protein (grafica c y d).
• increase in the expresión of the mRNA and the
Bcl-2 protein in the KGN group (graficas e y f).
• activation of the BID (grafica K) .

8. analysis of mitochondrial activity is evidenced in a
group of cells with visfatin and another group of
cells with FK866
• Visfatin treatment increases the
red network, indicating an
increase in potential..
Inhibitor treatment decreases the
red network and increases green
fluorescence, indicating organelle
inactivity.

9. DISCUSSION
autor What the autor said Agree or disagree
Li Visfatin is present in ascites fluid from ovarian cancer
patients, suggesting a connection with intraperitoneal
dissemination.
Wang We found downregulated expression of caspase3
mRNA, accompanied by decreased caspase-3 activity,
in
KGN and SKOV-3 cells. In addition, we showed
decreasing
cleavage of caspase-3 following visfatin treatment
Erfani visfatin decreases expression of pro-apoptotic proteins
(Bax and cleaved caspase) and simultaneously
increases expression of anti-apoptotic proteins (Bcl-2),
culminating in neuroprotective effects against ischemic
injury

10. CONCLUSIONS
• Molecular biology allows us to
understand how genes are expressed and
regulated in an organism. It also helps
identify genetic mutations that may be
associated with disease. This is essential
to understand the genetic causes of
diseases and to develop specific
therapies.
• Visfanthine becomes a risk factor
for ovarian cancer by stimulating
proliferation and inhibiting
apoptosis, decreasing the
expression of pro-apoptotic
proteins BAX and cleaved
caspase and increasing the
expression of anti-poptotic
proteins Bcl-2 affects the
intrinsic and extrinsic pathway of
the anokis.
targeting/inhibiting visfatin may be
a potential novel therapeutic
approach to inhibiting peritoneal
dissemination of ovarian carcinoma.

11. THANKS!