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Nwabr ethics jan 11

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Nwabr ethics jan 11

  1. 1. The Little Pathogen that Could Stefanie Iverson Cabral, PhD Totten Lab, Harborview Medical Center University of Washington Department of Medicine
  2. 2. The Little Pathogen that Could Mycoplasma genitalium <ul><li>Novel cause of reproductive tract disease </li></ul><ul><li>Minimal Microbe </li></ul><ul><li>Synthetic biology </li></ul>
  3. 3. Let’s talk about sex…
  4. 4. Male Urethritis Neisseria gonorrhoeae Chlamydia trachomatis Escherichia coli Herpes simplex virus 50% of male urethritis cases have an unknown cause!
  5. 5. Female Reproductive Tract Disease Neisseria gonorrhoeae Chlamydia trachomatis Escherichia coli Herpes simplex virus 60-70% of female cervicitis cases have an unknown cause!
  6. 6. Mycoplasma genitalium
  7. 7. Mycoplasma genitalium Persistence Cohen, et al. (2007) Sex Trans Dis. 34:274 Chronic infection = Mechanism(s) for immune evasion?
  8. 8. Who you calling “genomically reduced”?
  9. 10. Size doesn’t matter… <ul><li>4,639,675 base pairs </li></ul><ul><li>4267 genes </li></ul>E. coli <ul><li>580,076 base pairs </li></ul><ul><li>521 genes </li></ul>Mycoplasma genitalium Escherichia coli
  10. 11. Frankenstein Microbiology
  11. 12. Synthetic Biology http://www.jcvi.org/cms/research/projects/synthetic-bacterial-genome/press-release/
  12. 13. http://www.jcvi.org/
  13. 14. Frankenstein Microbiology Cells are the basic unit of life. Cells contain DNA. All of the DNA in a cell is a genome. DNA contains the instructions for life. Scientists first create synthetic DNA genome. A series of chemical reactions to “grow” DNA outside of a cell to produce an entire genome.
  14. 15. Scientists will order custom genomes. Craig Venter & the first-ever living organism made entirely of novel synthetic DNA. Mycoplasma genitalium as a template. Strip down Mycoplasma genitalium genome and then re-build using synthetic DNA. . Add human genes to “ minimal” bacterial genome. Synthia is born….
  15. 16. http://www.jcvi.org/
  16. 17. Science. 2010. 329:52-56
  17. 18. Science. 2010. 329:52-56
  18. 19. The Little Pathogen that Could Mycoplasma genitalium <ul><li>Novel cause of reproductive tract disease </li></ul><ul><li>Minimal Microbe </li></ul><ul><li>Synthetic biology </li></ul>
  19. 20. Totten Lab <ul><li>Raul Burgos </li></ul><ul><li>Laura Demaster </li></ul><ul><li>Laura Houston </li></ul><ul><li>Lisa Lowenstein </li></ul><ul><li>Raphael Ondondo </li></ul><ul><li>Nicole McFarland </li></ul><ul><li>Linda Arnesen </li></ul><ul><li>Sabina Astete </li></ul><ul><li>Heather Buckel </li></ul><ul><li>Gwen Wood </li></ul><ul><li>Jen Wroblewski </li></ul>
  20. 21. The Little Pathogen that Could Mycoplasma genitalium <ul><li>Thank you & Questions??? </li></ul>

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