1. SciFlowTM 1000 System
• Improved cell health and viability
• Biological relevance
• Gradient concentrations
• Multi-organ capabilities
• Broad compatibility

2. SciFlowTM 1000 Solution
Plate Readers
Dyes/Assays
High Content Imagers Each Plate tells a story
Gradients
Metabolites
Signaling
Biological Relevance Compatibility Data Rich
Benefits are biological, usability, and data-centric

3. SciFlowTM 1000 - Engineering
The simplicity is engineered into the system!
Complex Design User friendly solution
SciKon handled the engineering, so our customers could focus on the
biology

4. SciFlowTM 1000 Geometry
Gravity driven
No pumps or tubing (simple)
Entirely one material: polystyrene
SBS Compliant 96-well footprint
4mm

5. SciFlowTM 1000 Gradients
2 3 4 5 6 7 8 9 10 11
Test Compound
Metabolite
Flow
Each well mimics a full row on a static plate
Dose
As compound flows downstream…
Dilution effects
Cellular metabolism
Metabolites also flow
LC/MS id

6. SciFlowTM 1000 Flow
8 Rows/plate
multiple compounds
multiple concentrations
compound/controls
variable dosing
Cmpd
1
Cmpd
2
Cmpd
3

7. SciFlowTM 1000 Workflow
Feed the whole plate in 2 steps
1 – Remove media from the
sink in column 12
2 – Add fresh media/compound
into the source well
Frequency and volume of dosing is determined by the experiment.

8. Summary – Assays/Imagers/Readers
Compatible with all LC/MS instruments
Biochemical (Promega):
• Cell Titer Glo
• Cell Tox Green
High Content Imaging Dyes (ThermoScientific):
• Hoescht
Plate Readers:
• BMG CLARIOstar
Plate Imagers:
• Molecular Devices Image Express
• ThermoFisher Cellomics Imager
• CYP3A4
• Caspase 3/7
• CellRox Green• MitoTracker
Orange
• Tecan M1000Pro/Spark 10M
• Molecular Devices

9. SciFlowTM 1000 Applications
Liver Injury – Biologically Relevant Exposures
Compound Toxicity Testing – Concentration gradients
Metabolite Toxicity Testing – Drug vs. metabolite effects
Multi-Organ System Testing – Inter-cellular communication
Oral Exposure – Intestine  Liver  Target organ (i.e. heart)
Bioactivation of Chemotherapeutic – Intestine  Liver  Cancer cells
Cystic Fibrosis – Lung  Pancreas
Environmental Toxicants – Compound Screening
Inhalation/Skin/Oral – Lung, skin, and intestine screen
Flexible tool adaptable to many research areas