John wonders if the amount of sugar used affects the size of bread loaves. He formulates the hypothesis that more sugar means higher rising bread. John runs an experiment using different sugar amounts, keeping other ingredients and conditions constant. He finds that the control loaf with 50g sugar rises the highest, but 100g sugar works almost as well. John's initial hypothesis is rejected but further testing between 50-100g leads him to conclude that 70g sugar produces the largest loaf, supporting a modified hypothesis.