1. Barbara A. Roggenbeck, PhD
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Curriculum Vitae
Barbara A. Roggenbeck, Ph.D.
Personal information:
Citizenship: US
Mailing Address: 125 Laurel Ranch Rd,
Bozeman, MT, USA, 59715
Phone: (406) 589-4842
E-mail: roggenbe@ualberta.ca; barbararoggenbeck@gmail.com
Academic achievements:
 January 2009-December 2015: Completed Ph.D. in Physiology under the supervision of
Dr. Elaine M. Leslie at the University of Alberta, Edmonton, AB, Canada.
Thesis title: Hepatobiliary Transport of Arsenic
 January 2005- April 2008: Completed Master of Arts in Biology (Molecular Biology
track) under the supervision of Dr. Gregory Ahearn at the University of North Florida,
Jacksonville, FL, USA.
 December 2003- January 2005: Post-baccalaureate student under supervision of Dr.
Gregory Ahearn at the University of North Florida, Jacksonville, FL, USA.
 August 2001- December 2003: Completed Bachelor of Science in Biology from Florida
State University, Tallahassee, FL, USA.
Awards:
 2013: First place in oral presentation at the Department of Physiology Annual Research
Day, University of Alberta, Edmonton, AB, Canada.
 2009-2013: Alberta Innovates Health Solutions Studentship Award (formerly Alberta
Heritage Foundation for Medical Research) from AB, Canada. Full Stipend ($150,000)

2. Barbara A. Roggenbeck, PhD
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 2009: Graduate Student Recruitment Award from the Faculty of Medicine and Dentistry
at the University of Alberta, Edmonton, AB, Canada. Partial Stipend ($10,000)
 2008: Top Molecular and Cell Biology Student from the Department of Biology,
University of North Florida, Jacksonville, FL, USA.
 2008: Graduate Research Award from the Graduate Student Association, University of
North Florida, Jacksonville, FL, USA.
Teaching Experience:
 2011-2014: Physiology Graduate Student Tutorial Coordinator and Tutor for
undergraduate level Human Physiology course under supervision of Dr. Saswati Das at
the University of Alberta, Edmonton, AB, Canada.
 2007-2008: Graduate Teaching Assistant (undergraduate level Anatomy and Physiology
and General Biology laboratory courses) under the supervision of Dr. Cathy Paterson at
the University of North Florida, Jacksonville, FL, USA.
 2003: Undergraduate teaching assistant (undergraduate level Biology for non-majors
laboratory course) under supervision of Dr. Ann Lundstrom at Florida State University,
Tallahassee, FL, USA.
Related Extracurricular Experience:
 2012-2014: President of the Physiology Graduate Student Association at the University
of Alberta, Edmonton, AB, Canada.
 2011-2012: Vice President of the Physiology Graduate Student Association at the
University of Alberta, Edmonton, AB, Canada.
Book Publications:
1. Ahearn, G.A., Sterling, K.M., Mandal, P.K., and Roggenbeck, B.A. (2009) Heavy Metal
Transport and Detoxification by Crustacean Epithelial Lysosomes, IN: G.A. Gerenscer,
ed., Comparative epithelial transport processes, The Humana Press, Inc. pp. 49-71.

3. Barbara A. Roggenbeck, PhD
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2. Ahearn, G.A., Sterling, K.M., and Roggenbeck, B.A. (2009) Transport and
Detoxification of Heavy Metals by Invertebrate Epithelial Lysosomes. 4th
CPB Meeting in
Africa: Mara 2008. “Molecules to migration: The pressures of life” (Ed.S. Morris & A.
Vosloo) Medimond Publishing Co., via Maserati 6/2, 401241 Bologna, Italy, pp 61-74.
Journal Publications:
1. Roggenbeck, B.A., Carew, M.W., Charrois, G.J., Douglas, D.N., Kneteman, N.M., Lu,
X. Le, X.C., and Leslie, E.M. (2015) Characterization of Arsenic Hepatobiliary
Transport Using Sandwich –Cultured Human Hepatocytes. Toxicol. Sci. 145(2): 307-
320. Editor’s highlight.
2. Banerjee, M., Carew, M.W., Roggenbeck, B.A., Whitlock, B.D., Naranmandura, H., Le,
X.C., and Leslie, E.M. (2014) A Novel Pathway for Arsenic Elimination: Human
Multidrug Resistance Protein 4 (MRP4/ABCC4) Mediates Cellular Export of
Dimethylarsinic Acid (DMAV
) and the Diglutathione Conjugate of Monomethylarsonous
acid (MMAIII
). Mol Pharmacol 86: 168-174.
3. Sterling, K.M., Roggenbeck, B.A., and Ahearn, G.A., (2010) Dual Control of Cytosolic
Metals by Lysosomal Transporters in Lobster Hepatopancreas. J. Exp. Biol. 213:769-
774.
4. Sterling, K.M., Mandal, P.K., Roggenbeck, B.A., Ahearn, S.E., Gerenscer, G.A., and
Ahearn, G.A. (2007) Heavy Metal Detoxification in Crustacean Epithelial Lysosomes:
Role of anions in the compartmentalization process. J Exp. Biol. 210:3484-3493.
Invited Talks:
 Roggenbeck, B.A. Transport of Arsenic by Sandwich Cultured Human Hepatocytes.
First Annual Toxicology Symposium, University of Alberta, Edmonton, Canada.
September 25, 2013.
Abstracts:
Oral Presentations:

4. Barbara A. Roggenbeck, PhD
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1. Roggenbeck, B.A., Banerjee, M., Carew, M.W., Whitlock, B.D., and Leslie, E.M.
Hepatobiliary Transport of Arsenic by the Human Multidrug Resistance Proteins. 4th
Asian Conference on Environmental Mutagens, Kolkata, India, Dec 10-12th, 2014.
(presented by Dr. Elaine M. Leslie)
2. Banerjee, M., Carew, M.W., Roggenbeck, B.A., Whitlock, B.D., and Leslie, E.M.
Human Multidrug Resistance Protein 4 (MRP4) Plays a Critical Role in Transport of
Arsenic Metabolites. 22nd
Annual Physiology Research Day, Royal Mayfair Golf Course,
Edmonton, AB, Canada, March 14, 2014. (presented by Dr. Mayukh Banerjee)
3. Roggenbeck, B.A., Carew, M.W., Charrois, G.J., and Leslie, E.M. Characterization of
Arsenic Transport in Sandwich Cultured Human Hepatocytes. 22nd
Annual Physiology
Research Day, University of Alberta, Edmonton, Canada, March 14, 2014.
4. Roggenbeck, B.A, Carew, M.W., Charrois, G.J., and Leslie, E.M. Arsenic Transport
Studies Using Sandwich Cultured Human Hepatocytes. Membrane Protein Disease
Research Group, University of Alberta, Edmonton, AB, Canada, January 30, 2014.
5. Roggenbeck, B.A., Carew, M.W., Swanlund, D., Banerjee, M., and Leslie, E.M.
Hepatobiliary Transport of Arsenic by the Multidrug Resistance Proteins. 10th
International Symposium of Persistent Toxic Substances. University of Alberta,
Edmonton, AB, Canada. August 13-17, 2013. (published) (presented by Dr. Elaine
Leslie)
6. Roggenbeck, B.A., Carew, M.W., Charrois, G.J., and Leslie, E.M. Arsenic Transport
Studies in Sandwich Cultured Human Hepatocytes. 21st
Annual Physiology Research
Day, University of Alberta, Edmonton, Canada. March 21, 2013.
7. Roggenbeck, B.A., Carew, M.W., Charrois, G.J., and Leslie, E.M. Arsenic Transport in
Sandwich Cultured Human Hepatocytes. Membrane Protein Research Group, University
of Alberta, Edmonton, Canada, January 12, 2012.
8. Roggenbeck, B.A. and Leslie, E.M. Cellular Handling of Carcinogens. Department of
Physiology, University of Alberta, Edmonton, Canada, November 30, 2010.
Poster Presentations:
1. Banerjee, M., Carew, M.C., Roggenbeck, B.A., Whitlock, B.D., and Leslie, E.M. Role of
Human Multidrug Resistance Protein 4 (MRP4) in Arsenic Transport. Prarie Northern

5. Barbara A. Roggenbeck, PhD
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Chapter of the Society of Environmental Toxicology and Chemistry North America,
University of Saskatchewan, Saskatoon, Saskatchewan. June 6, 2014.
2. Lee, H., Banerjee, M., Roggenbeck, B.A., and Leslie, E.M. Multidrug Resistance Protein
2 (MRP2) Genetic Variants and the Hepatobiliary Transport of Arsenic. Faculty of
Medicine and Dentistry Summer Student Research Day. September 2014.
3. Banerjee, M., Carew, M.C., Roggenbeck, B.A., Whitlock, B.D., and Leslie, E.M.
Human Multidrug Resistance Protein 4 (MRP4/ABCC4) Mediates Cellular Export of
Dimethylarsinic acid (DMAV
) and the Diglutathione Conjugate of Monomethylarsonous
acid (MMA III
). International Research Training Group/Membrane Protein Research
Group. Banff, Alberta, Canada. April 8, 2014.
4. Roggenbeck, B.A., Carew, M.W., Charrois, G.J., and Leslie, E.M. Hepatobiliary
Transport of Arsenic in Sandwich Cultured Human Hepatocytes. 10th
International ISSX
Meeting, Toronto, ON, Canada. September 29-October 3, 2013. Drug Metab Rev.
45(1):261-262. (published).
5. Roggenbeck, B.A., Carew, M.W., Charrois, G.J., and Leslie, E.M. Hepatobiliary
Transport of Arsenic in Sandwich Cultured Human Hepatocytes. 1st
Annual Toxicology
Symposium, University of Alberta, Edmonton, Canada. September 25, 2013.
6. Roggenbeck, B.A., Carew, M.W., Charrois, G.J., and Leslie, E.M. Transport of Arsenic
by Human Hepatocytes. 10th
International Symposium on Persistent Toxic Substances
(ISPTS). University of Alberta, Edmonton, Canada. August 13- 17, 2013.
7. Roggenbeck, B.A., Carew, M.W., Leslie, E.M. Arsenic Transport Studies with
Sandwich Cultured Human Hepatocytes. 18th
North American Regional ISSX Meeting.
Dallas, Texas. October 14-18, 2012. Drug Metab Rev. 44(1): 149. (published).
8. Roggenbeck, B.A., Carew, M.C., Sahajpal, R., Charrois, G.A., and Leslie, E.M.
Hepatobiliary transport of arsenic by the MRPs. Faculty Research and Development
Day, Faculty of Pharmacy and Pharmaceutical Sciences. University of Alberta,
Edmonton, Canada. November 19, 2010.
9. Roggenbeck, B.A., Carew, M.C., Sahajapal, R., Charrois, G.A., and Leslie, E.M.
Hepatic transport of arsenic by the MRPs. Alberta/Germany Joint Membrane Transport
Symposium. Kaiserslautern,Germany. September 21-24, 2010.
10. Roggenbeck, B.A., Witkowska, K., O’Neill, D., Cheeseman, C., and Leslie, E.M.
Tobacco carcinogen 4-(methylnitrosamino)-1-(3- pyridyl) butanone transport in Xenopus

6. Barbara A. Roggenbeck, PhD
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laevis oocytes. 18th
Annual Physiology Research Day. University of Alberta, Edmonton,
Canada. April 7, 2010.
11. Roggenbeck, B.A., and Leslie, E.M. Tobacco Carcinogen Transport by the Human
Multidrug Resistance Proteins. 17th
Annual Physiology Research Day. University of
Alberta, Edmonton, Canada. March 26, 2009.
12. Roggenbeck, B.A., Sterling, K.M., Ahearn, G.A. (2008) Role of Metal-Thiol Conjugates
in Heavy Metal Detoxification. FASEB J. 22(12): 1202.8 (published).
13. Roggenbeck, B.A., Mandal, P.K., Ahearn, G.A. (2006) Role of Polyvalent Anions in
Lysosomal Heavy Metal Detoxification. FASEB J. 20(5): A1236 (published).
Research Skills:
Biochemical techniques:
1. Immunoblotting (Western and Dot blots)
2. Membrane protein characterization (inside-out plasma membrane vesicle synthesis,
radioactive and non-radioactive transport assays)
3. Kinetic characterization of proteins (Km, Vmax calculations)
4. HPLC (and ICP-MS for transport assays)
5. TLC
Molecular and Cell Biology techniques:
1. Recombinant DNA techniques (plasmid isolation and purification, subcloning, DNA
sequences, PCR)
2. Mammalian stable cell line production (HEK293 stable cell lines and functional
characterization)
3. Mammalian cell culture (transient transfections for short term protein expression)
4. Human hepatocyte isolation and sandwich culture
5. Rat hepatocyte culture (from cryopreserved cells)
6. Cytotoxicity assays (MTS and LDH assays)
7. Protein assays (Bradford and BCA)

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8. Immunocytochemistry (confocal imaging slide preparation)
9. Gene silencing (lentiviral particle production and concentration , shRNA silencing in
primary human hepatocytes, shRNA silencing in mammalian cell culture)
10. ELISA assays (p24 for viral titer)
Laboratory Management Skills:
1. Ordering and organization of lab supplies
2. Isotope inventory and disposal
3. Hazardous waster disposal
4. Design of radioisotope experiments (73
As and 3
H)