This document discusses the impacts of roads on wildlife habitat and populations. It notes that roads can increase mortality for some species like tigers and panthers due to vehicle collisions, poaching, and reduced prey availability. Roads also fragment habitat and can act as barriers that isolate populations. The document reviews studies on the Amur tiger that found higher mortality near roads and presents the Florida panther as a case study, where roads and vehicle collisions account for nearly half of deaths. Conservation efforts aim to reduce road impacts through prevention of unnecessary construction and regulation of access.