This was a project I designed while working in Tim Devoogd\'s lab at Cornell University on a new technique to study how memory gets formed in the brain.Read less
![Purpose This study was conducted to determine hippocampal outputs by using a gene gun to apply multiple dyes in order to explore interneuronal connections at the systematic level. Tracing Hippocampal Memory Formation in the European Starling Daniel Katz Devoogd Lab, Department of Neurobiology/Psychology, Cornell University Acknowledgements The author acknowledges Cornell University and the Rawlings Cornell Presidential Scholarship (RCPRS) program for funding this research. This work was possible thanks to Timothy Devoogd and L. Matthew Law for their continued guidance and assistance throughout this project. ,[object Object],[object Object],Why the European Starling? What do we mean by memory? Introduction Methods – Gene Gun ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Potential Results Discussion Hippocampus Diagram drawn by Timothy Devoogd. Bio-Rad's portable Helios Gene Gun This image shows an example of the individual types of neurons targeted by the specific dye combinations (left), and a region where multiple cells of different types are present and visually distinguishable (right). Image taken from Gan et al., 2000, published in Neuron. ,[object Object],[object Object],Introduction Methods – Gene Gun Methods – Neuronal Labeling Structure of Lipophilic carbo-cyanine dyes Dil and DiO. Diagram showing the mechanism of the gene gun delivery system. Particles are propelled towards the cell by helium pulses, which then disperse as the particles penetrate the cell. The low pressure needed to propel the particles and the filter prevent damage to the cell. Picture taken from O’brien & Lumis, Trends in Biotechnology. Diagram drawn by Timothy Devoogd Picture by Sam Crowe, Cornell Lab of Ornithology T e mporal lobe Hippocampus Picture by Sam Crowe, Cornell Lab of Ornithology](https://image.slidesharecdn.com/researchpresentation-12524449876285-phpapp01/85/Poster-1-320.jpg)
