Food analysis, protein, amino acid , pharmaceutical validation, m.pharm, pharmaceutical analysis, pharmaceutics, drug analysis

1. PROTEIN
(FOOD ANALYSIS)
PRESENTED TO,
Dr. Harsha K. Tripathy
Professor,
Dept. of pharmaceutical analysis,
Karnataka college of pharmacy,
Bangalore.
PRESENTED BY,
R.Venkatesan,
M.Pharm 1st semester,
Karnataka college of pharmacy,
Bangalore,
27/02/2023

2. Introduction
01
Chemistry
02
Classification
03
Digestion and absorption
04
Content
Metabolism
05
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3.  Organic compound
 Molecules which yields amino acids from hydrolysis
are called as proteins
 Protein are made up of carbon, hydrogen, nitrogen
and some Sulphur
 It is a building blocks of life
 Proteins are higher molecular weight
PROTEIN
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4. • Chemically protein are polymers of amino acids (it is a
building material of protein)
• Protein are mainly constituted by five major elements.
 Carbon 50-55%
 Hydrogen 6-7.3%
 Oxygen 19-24%
 Nitrogen 13-19%
 Sulfur 0-4%
Chemistry
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5. • Each amino acids has one central tetrahedral carbon atom to which three
functional group are bound
1. Amino group (-NH2)
2. Acidic carboxyl (-COOH)
3. Side chain group (-R)
• So there are four different groups attached to alpha carbon atom
• It is called chiral center or asymmetric carbon and exhibit optical
isomerism.
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6. Examples
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7. • Primary structure
• Secondary structure
• Tertiary structure
• Quaternary structure
STRUCTURE OF PROTEIN
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10. Classification based on chemical nature an solubility
I ) Simple protein-Composed of only amino acids
residue
Globular protein – soluble in water and other
solvent
Ex:
albumins, globulins, glutelines, histones, globins,
protamines.
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12. ii). Conjugated proteins
contains amino acids and non protein moiety.
Ex:
nucleoproteins, glycoproteins, lipoproteins,
phosphoproteins, chromoproteins, mettalloproteins
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13. iii)Derived proteins
Denaturated or degraded product of simple and
conjucated proteins
1.primary- conjucated protein, protones, metaproteins
2.Secondary – proteoses, peptones, polypeptides,
peptides
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14. Digestion of Protein
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15. Amino acids are absorbed from the intestinal lumen through
secondary active Na+ dependent transport systems and through
facilitated diffusion.
ABSORPTION:
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16. Catabolism
METABOLISM OF PROTEIN
CATABOLISM IS THE SET OF METABOLIC
PATHWAYS THAT BREAKS DOWN
MOLECULES INTO SMALLER UNITS THAT
ARE EITHER OXIDIZED TO RELEASE
ENERGY OR USED IN OTHER ANABOLIC
REACTIONS..
ANABOLISM IS THE SET OF METABOLIC
PATHWAYS THAT CONSTRUCT
MOLECULES FROM SMALLER UNITS,
THESE REACTIONS REQUIRE ENERGY,
KNOWN ALSO AS AN ENDERGONIC
PROCESS..
Anabolism
Metabolism is the
process by which the
body changes food and
drink into energy.
Metabolism
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17. •CATABOLISM OF PROTEIN
Catabolism of amino
acids involves the removal of
the amino group, followed by
the breakdown of the resulting
carbon skeleton
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19. •ANABOLISM OF PROTEIN
Protein anabolism is the process by
which proteins are formed from amino
acids. Proteins are made from amino
acids. In humans, some amino acids can
be synthesized using already existing
intermediates. These amino acids are
known as non-essential amino acids.
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20. SYMPTOMS OF PROTEIN DEFICIENCY
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21. • High protein consumption may cause
High-protein diets may increase the risk of coronary
artery disease.
cancer and osteoporosis.
Vitamin B deficiencies can develop if the diet is followed
chronically. It may include bad breath, constipation,
fatigue, and nausea.
High protein foods are beef, chicken, eggs etc.
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22. PROTEIN RICH FOODS
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23. Important question
1. Write in detail about digestion of proteins and absorption
proteins.
2. Write about primary, secondary and tertiary structure of
proteins.
3. Explain briefly about physicochemical properties of
proteins and their structure.
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24. THANK YOU