Prof Cannas Reference
•
0 likes•29 views
This letter is a recommendation for Michaela Agrifoglio from her former professor Rita Cannas. Professor Cannas supervised Michaela's master's thesis project at the University of Cagliari, where Michaela studied marine bioecology. Michaela demonstrated excellent skills in molecular biology techniques like DNA extraction and PCR. Her research project aimed to identify genetic variability in populations of the longnose skate in the Sea of Sardinia to inform conservation efforts. Professor Cannas recommends Michaela for an internship or PhD program based on her strong research and interpersonal skills shown during her master's work.
Report
Share
Report
Share
Download to read offline
Recommended
updated cv
Maram Ahmed Adel El Demerdash has a Bachelor's degree in Biotechnology and Biomolecular Chemistry from Cairo University, where she maintained a GPA of 3.263. She has experience in biotechnology research projects, conferences, and coursework covering areas such as molecular biology techniques, microbiology, and fermentation. Currently she works as a medical representative while continuing biotechnology research as a graduate student.
cover letter two
Michaela Agrifoglio holds a master's degree in Marine Bio-ecology. She conducted research at a genetics lab aimed at preserving endangered species, including identifying possible genetic variability and mutations that could threaten species' well-being. Her thesis studied the Dipturus oxyrinchus fish species in the Sea of Sardinia to identify genetic variability between populations. Through committed work, she obtained results analyzing the species' nucleotide sequences and identifying possible genetic variability between areas. She is interested in further research opportunities to preserve and protect more species from potential threats.
resume two
Michaela Agrifoglio is seeking to use her biological and laboratory skills in an Australian workplace. She has a Master's Degree in Marine Bioecology and believes all marine species and environments must be protected. She hopes to apply her knowledge and skills in Australia to contribute to marine biology and species conservation overseas. Her work experience includes internships analyzing genetic variation in monkfish populations and arachnology, as well as environmental education and open water diving.
Prof Deiana Reference
Prof. Anna Maria Deiana is writing a letter of recommendation for her former student, Michaela Agrifoglio. Michaela completed a Bachelor's Degree in Applied Bioecology and worked in Prof. Deiana's laboratory for six months to fulfill her training requirements, where she learned spider sampling techniques and biological identification skills. Prof. Deiana notes that Michaela progressed quickly, demonstrated adaptability and enthusiasm for learning, and has strong skills in taxonomic determination, data analysis, and organization. She concludes that Michaela is a motivated student committed to biodiversity protection.
Cv sandra diaz garcia april 2016
Sandra Diaz-Garcia is a postdoctoral fellow at the University of California San Diego studying the genetic mechanisms underlying regeneration and wound healing. She received her Ph.D. from Universidad Autónoma de Madrid focusing on developing a technique to study regeneration in Drosophila imaginal discs. Her current projects involve identifying genetic pathways controlling wound repair in Drosophila embryos and analyzing regeneration in the fly central nervous system using microscopy. She has extensive experience in genetics, molecular biology, microscopy, and Drosophila techniques.
Senegal dairy genetics: Using advanced DNA approaches to determine breed comp...
Poster prepared by Karen Marshall, Stanly Tebug, Jarmo Juga, Miika Tapio and Ayao Missohou for the Workshop on Food and Nutrition Security in Africa, Helsinki, Finland, 16 June 2014
UCC IGEM BROCHURE
The document summarizes the iGEM competition and the projects of University College Cork's 2014 iGEM team. The iGEM competition is an annual synthetic biology competition for undergraduate students. UCC's 2014 team developed two projects: Operation Hagfish aims to mass produce a biopolymer from hagfish slime in bacteria, while Biagnostics develops a novel DNA diagnostic method using bacteria. The team's goal is to present their work at the 2014 iGEM Jamboree in Boston and compete for prizes across various categories.
SPONGES AND THEIR POTENTIAL USES by Ranjith Edirisinghe
SPONGES AND THEIR POTENTIAL USES by Ranjith Edirisinghe Presented during the Regional Workshop on Underutilized Fish and Marine Genetic Resources and their Amelioration, 10-12 July 2019, Location: Colombo, Sri Lanka
Recommended
updated cv
Maram Ahmed Adel El Demerdash has a Bachelor's degree in Biotechnology and Biomolecular Chemistry from Cairo University, where she maintained a GPA of 3.263. She has experience in biotechnology research projects, conferences, and coursework covering areas such as molecular biology techniques, microbiology, and fermentation. Currently she works as a medical representative while continuing biotechnology research as a graduate student.
cover letter two
Michaela Agrifoglio holds a master's degree in Marine Bio-ecology. She conducted research at a genetics lab aimed at preserving endangered species, including identifying possible genetic variability and mutations that could threaten species' well-being. Her thesis studied the Dipturus oxyrinchus fish species in the Sea of Sardinia to identify genetic variability between populations. Through committed work, she obtained results analyzing the species' nucleotide sequences and identifying possible genetic variability between areas. She is interested in further research opportunities to preserve and protect more species from potential threats.
resume two
Michaela Agrifoglio is seeking to use her biological and laboratory skills in an Australian workplace. She has a Master's Degree in Marine Bioecology and believes all marine species and environments must be protected. She hopes to apply her knowledge and skills in Australia to contribute to marine biology and species conservation overseas. Her work experience includes internships analyzing genetic variation in monkfish populations and arachnology, as well as environmental education and open water diving.
Prof Deiana Reference
Prof. Anna Maria Deiana is writing a letter of recommendation for her former student, Michaela Agrifoglio. Michaela completed a Bachelor's Degree in Applied Bioecology and worked in Prof. Deiana's laboratory for six months to fulfill her training requirements, where she learned spider sampling techniques and biological identification skills. Prof. Deiana notes that Michaela progressed quickly, demonstrated adaptability and enthusiasm for learning, and has strong skills in taxonomic determination, data analysis, and organization. She concludes that Michaela is a motivated student committed to biodiversity protection.
Cv sandra diaz garcia april 2016
Sandra Diaz-Garcia is a postdoctoral fellow at the University of California San Diego studying the genetic mechanisms underlying regeneration and wound healing. She received her Ph.D. from Universidad Autónoma de Madrid focusing on developing a technique to study regeneration in Drosophila imaginal discs. Her current projects involve identifying genetic pathways controlling wound repair in Drosophila embryos and analyzing regeneration in the fly central nervous system using microscopy. She has extensive experience in genetics, molecular biology, microscopy, and Drosophila techniques.
Senegal dairy genetics: Using advanced DNA approaches to determine breed comp...
Poster prepared by Karen Marshall, Stanly Tebug, Jarmo Juga, Miika Tapio and Ayao Missohou for the Workshop on Food and Nutrition Security in Africa, Helsinki, Finland, 16 June 2014
UCC IGEM BROCHURE
The document summarizes the iGEM competition and the projects of University College Cork's 2014 iGEM team. The iGEM competition is an annual synthetic biology competition for undergraduate students. UCC's 2014 team developed two projects: Operation Hagfish aims to mass produce a biopolymer from hagfish slime in bacteria, while Biagnostics develops a novel DNA diagnostic method using bacteria. The team's goal is to present their work at the 2014 iGEM Jamboree in Boston and compete for prizes across various categories.
SPONGES AND THEIR POTENTIAL USES by Ranjith Edirisinghe
SPONGES AND THEIR POTENTIAL USES by Ranjith Edirisinghe Presented during the Regional Workshop on Underutilized Fish and Marine Genetic Resources and their Amelioration, 10-12 July 2019, Location: Colombo, Sri Lanka
NIKHIL_NIKUNJ_MSc_PAPER
This document summarizes a study that used DNA barcoding to characterize Niphargus Kochianus Irlandicus, a tiny crustacean found in groundwater in Ireland. DNA was extracted from legs of specimens collected from two sites and amplified for the COI gene. Sequencing of the COI gene from four specimens revealed 72-84% homology with other Niphargus species, indicating molecular divergence within the species. A phylogenetic tree constructed from the COI sequences showed the existence of hidden diversity within Niphargus Kochianus Irlandicus.
Food Standards Australia New Zealand
The student was given an unknown mixed culture and used quadrant streaking to isolate a pure colony. A Gram stain showed the isolated bacteria was Gram positive. Motility tests found it was non-motile. A series of metabolic tests for properties like citrate utilization, carbohydrate fermentation, and catalase presence were done. By comparing the results to database entries, the unknown bacteria was identified as Staphylococcus.
CV only
This document contains a summary of Sandra G. Nishikawa's skills and experience. She has over 30 years of experience in molecular biology techniques including DNA/RNA isolation, PCR, cloning, sequencing, cell culture and more. She has worked as a research technician for various professors at the University of Calgary studying topics like hepatitis B virus, prion diseases, and cancer.
Biogenic antimicrobial silver nanoparticles produced by fungi
The document summarizes a study that found two fungi isolated from a Brazilian mangrove forest were able to extracellularly synthesize spherical silver nanoparticles (Ag NP) that were 35±10 nm in size. The Ag NP were characterized through various techniques and showed pronounced antimicrobial activity against pathogenic fungi and bacteria. In particular, Ag NP produced by Aspergillus tubingensis were highly effective at inhibiting Pseudomonas aeruginosa growth. This is the first report of extracellular Ag NP synthesis and antimicrobial properties of Ag NP produced by fungi isolated from a Brazilian mangrove, opening possibilities for obtaining biogenic Ag NP with positive surface potential and new applications.
Jones_Robyn_MEP
This thesis investigates the effects of microplastic PVC particles and the pollutant
fluoranthene on the lugworm Arenicola marina and whether a transfer could occur through a
model food chain of mussel Mytilus edulis and dog whelk Nucella lapillus. Arenicola marina
were exposed to different PVC concentrations and pollution levels, observing impacts on
faecal casts, weight, and mortality. Mytilus edulis were then exposed to PVC and presented
to dog whelks to observe particle transfer. Results showed Arenicola marina was affected at
higher PVC levels but resilient to lower levels. Particle transfer was observed between
mus
Literature Review Madihah Ismail PSP3 2024
This document provides a literature review on the evolution and applications of cloning technology in conservation strategies for endangered species. It discusses key milestones like the cloning of Dolly the sheep and advances in genome editing. The review examines case studies on using cloning to rescue species on the brink of extinction. While cloning holds promise for preserving biodiversity, challenges and ethical considerations must be addressed. Responsible use of these innovative techniques could help safeguard genetic diversity if balanced with environmental and social impacts.
Tesis final alejandro moya
This document presents a research project that evaluated the antifungal activity of mixtures of aqueous extracts from Amazonian plants against Candida albicans and Aspergillus niger fungi. Ultrasound-assisted extraction was used to obtain aqueous extracts rich in polyphenols from Cedrela odorata, Psidium guajava, and Ocotea quixos plants. Total polyphenol content was determined and the extracts were found to contain 2-4g polyphenols/100g solids. A statistical mixture design was used to evaluate the antifungal activity of extract mixtures. The mixtures showed effectiveness against C. albicans but not A. niger. Certain proportions produced potentiated ant
MESTECH_October_2012
The newsletter summarizes activities from MESTECH researchers over the past few months, including presenting research at several conferences on topics like environmental sensing, water quality monitoring, and nanomaterials. It also describes a successful collaborative conference between MESTECH and the University of Notre Dame on developing sensing technologies for environmental challenges.
MSc dissertation FINAL Timea Grego
This document is a dissertation submitted by Timea Grego for the degree of Master of Science in Environmental Systems Engineering. The dissertation evaluates the use of fine sand filtration for large-scale purification of alginate for clinical applications. Alginate is a natural polymer extracted from seaweed that is used to microencapsulate liver cells. However, impurities must be removed for regulatory approval. The study aimed to determine if sand filtration could remove particulates from alginate solutions without altering the physical properties. The results showed that a dual media filter removed more particulates than a single medium filter, but filtration negatively impacted the viscosity of alginate solutions, altering hydrogel properties. In conclusion, sand filtration was not a suitable method
Rs&C G3(7 Fp)
The document summarizes the research of Rafael Giraldo's group, which aims to develop microbial model systems for applications related to protein amyloidosis. The group studies ligand-induced conformational switches in proteins and designs protein devices to control DNA replication and amyloid assembly. Current projects examine conformational activation of protein-DNA complexes and new microbial models for protein amyloidosis. The group collaborates with other researchers and has expertise in cell biology, molecular biology, biophysics, and screening techniques like fluorescence microscopy and spectroscopy.
Hepato Protective Assessment of Pawpaw Leaves, Neem, Lemon Grass and Acts on ...
Malaria is a major concern in Nigeria, and stands as the second leading cause of death from all infectious disease in Africa. Several studies have reported the damaging effect of the parasite to various body organs especially the liver. Reports over time has shown the benefits of various plants extracts in ethno medicine. However, not much have been done on the effects of some of these extracts in combined form on its hepato protective assessment in comparison with any known ACT based anti malaria. The focus of this study was to explore the hepato protective properties of ethanoic extract of Carica papaya Linn, AzadirachtaIndica, CymbopogonCitratusagainst ACT based antimalarial therapy on plasmodium berghei parasitized wistar rats. Phytochemical analysis of the extracts were done according to the method described by Treaseand Evans. Hepato protective assessment were done using the liver function tests and assay of the liver histology respectively. One hundred and ten 110 rats distributed into 11 groups, each group having 10rats were used for the experiment. Negative control received just feed and water, Positive control were induced with the malaria parasite and given feed and water only. The tests groups were induced with malaria, received feed and water and treated with 500mg kg, 250mg kg and 165mg kg doses of the extracts, both individually and in combined forms, as well as the standard ACT anti malaria. Phytochemical screening showed that the plant extracts possessed high concentration of Tannins, Flavonoids, Saponins and Alkaloids. Plasmodium berghei increased the activities of ALP, ASP and ALT when compared with the positive control group. This may be attributed to increase in functional capacity of the liver as a result of the presence of the infection for the tests groups. Treatment with the plant extracts decreased ALP and ALT levels significantly P 0.05 , as well as AST levels except for the Neem extract. Histological examination of the liver of test animals showed no extensive damage to the tissue by the individual extracts when compared to the negative control group. Nnyaha Anthonia E. | Igbokwe Ugochukwu V. | Okonkwo Onyeka Chukwudi | Ajeka Prisca O. | Nwaissac Ikechukwu S. | Okpa Precious N. "Hepato-Protective Assessment of Pawpaw Leaves, Neem, Lemon Grass and Acts on Plasmodium Berghei Parasitized Wistar Rats" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46292.pdf Paper URL: https://www.ijtsrd.com/medicine/physiology/46292/hepatoprotective-assessment-of-pawpaw-leaves-neem-lemon-grass-and-acts-on-plasmodium-berghei-parasitized-wistar-rats/nnyaha-anthonia-e
Testing for COVID-19 with Antibody and PCR Tests in Low Resource Areas
One of the recommendations for mass testing developed by UPM and USA researchers backed by PAASE sent to DOH awaiting possible adoption
Meagan Hicks CV 2016
Meagan Hicks has a Bachelors of Science in Biochemistry from the University of New Brunswick. She has work experience researching Spartina grasses in salt marsh restoration and researching metabolism genetics in fruit flies. Her skills include experimental design, statistical analysis, bioinformatics, and laboratory techniques. She is proficient in French and has knowledge of German, Spanish, and Mandarin.
2014 SUCR Poster
1. The researchers collected frog egg masses from bodies of water in Illinois to isolate water mold pathogens.
2. They obtained DNA sequences from the ITS region of fourteen water mold isolates and classified the isolates using phylogenetic analysis.
3. The researchers designed a locked nucleic acid primer/probe set for quantitative PCR (qPCR) to identify a specific Saprolegnia strain. They are currently testing the probe's ability to only amplify the desired strain.
More Related Content
Similar to Prof Cannas Reference
NIKHIL_NIKUNJ_MSc_PAPER
This document summarizes a study that used DNA barcoding to characterize Niphargus Kochianus Irlandicus, a tiny crustacean found in groundwater in Ireland. DNA was extracted from legs of specimens collected from two sites and amplified for the COI gene. Sequencing of the COI gene from four specimens revealed 72-84% homology with other Niphargus species, indicating molecular divergence within the species. A phylogenetic tree constructed from the COI sequences showed the existence of hidden diversity within Niphargus Kochianus Irlandicus.
Food Standards Australia New Zealand
The student was given an unknown mixed culture and used quadrant streaking to isolate a pure colony. A Gram stain showed the isolated bacteria was Gram positive. Motility tests found it was non-motile. A series of metabolic tests for properties like citrate utilization, carbohydrate fermentation, and catalase presence were done. By comparing the results to database entries, the unknown bacteria was identified as Staphylococcus.
CV only
This document contains a summary of Sandra G. Nishikawa's skills and experience. She has over 30 years of experience in molecular biology techniques including DNA/RNA isolation, PCR, cloning, sequencing, cell culture and more. She has worked as a research technician for various professors at the University of Calgary studying topics like hepatitis B virus, prion diseases, and cancer.
Biogenic antimicrobial silver nanoparticles produced by fungi
The document summarizes a study that found two fungi isolated from a Brazilian mangrove forest were able to extracellularly synthesize spherical silver nanoparticles (Ag NP) that were 35±10 nm in size. The Ag NP were characterized through various techniques and showed pronounced antimicrobial activity against pathogenic fungi and bacteria. In particular, Ag NP produced by Aspergillus tubingensis were highly effective at inhibiting Pseudomonas aeruginosa growth. This is the first report of extracellular Ag NP synthesis and antimicrobial properties of Ag NP produced by fungi isolated from a Brazilian mangrove, opening possibilities for obtaining biogenic Ag NP with positive surface potential and new applications.
Jones_Robyn_MEP
This thesis investigates the effects of microplastic PVC particles and the pollutant
fluoranthene on the lugworm Arenicola marina and whether a transfer could occur through a
model food chain of mussel Mytilus edulis and dog whelk Nucella lapillus. Arenicola marina
were exposed to different PVC concentrations and pollution levels, observing impacts on
faecal casts, weight, and mortality. Mytilus edulis were then exposed to PVC and presented
to dog whelks to observe particle transfer. Results showed Arenicola marina was affected at
higher PVC levels but resilient to lower levels. Particle transfer was observed between
mus
Literature Review Madihah Ismail PSP3 2024
This document provides a literature review on the evolution and applications of cloning technology in conservation strategies for endangered species. It discusses key milestones like the cloning of Dolly the sheep and advances in genome editing. The review examines case studies on using cloning to rescue species on the brink of extinction. While cloning holds promise for preserving biodiversity, challenges and ethical considerations must be addressed. Responsible use of these innovative techniques could help safeguard genetic diversity if balanced with environmental and social impacts.
Tesis final alejandro moya
This document presents a research project that evaluated the antifungal activity of mixtures of aqueous extracts from Amazonian plants against Candida albicans and Aspergillus niger fungi. Ultrasound-assisted extraction was used to obtain aqueous extracts rich in polyphenols from Cedrela odorata, Psidium guajava, and Ocotea quixos plants. Total polyphenol content was determined and the extracts were found to contain 2-4g polyphenols/100g solids. A statistical mixture design was used to evaluate the antifungal activity of extract mixtures. The mixtures showed effectiveness against C. albicans but not A. niger. Certain proportions produced potentiated ant
MESTECH_October_2012
The newsletter summarizes activities from MESTECH researchers over the past few months, including presenting research at several conferences on topics like environmental sensing, water quality monitoring, and nanomaterials. It also describes a successful collaborative conference between MESTECH and the University of Notre Dame on developing sensing technologies for environmental challenges.
MSc dissertation FINAL Timea Grego
This document is a dissertation submitted by Timea Grego for the degree of Master of Science in Environmental Systems Engineering. The dissertation evaluates the use of fine sand filtration for large-scale purification of alginate for clinical applications. Alginate is a natural polymer extracted from seaweed that is used to microencapsulate liver cells. However, impurities must be removed for regulatory approval. The study aimed to determine if sand filtration could remove particulates from alginate solutions without altering the physical properties. The results showed that a dual media filter removed more particulates than a single medium filter, but filtration negatively impacted the viscosity of alginate solutions, altering hydrogel properties. In conclusion, sand filtration was not a suitable method
Rs&C G3(7 Fp)
The document summarizes the research of Rafael Giraldo's group, which aims to develop microbial model systems for applications related to protein amyloidosis. The group studies ligand-induced conformational switches in proteins and designs protein devices to control DNA replication and amyloid assembly. Current projects examine conformational activation of protein-DNA complexes and new microbial models for protein amyloidosis. The group collaborates with other researchers and has expertise in cell biology, molecular biology, biophysics, and screening techniques like fluorescence microscopy and spectroscopy.
Hepato Protective Assessment of Pawpaw Leaves, Neem, Lemon Grass and Acts on ...
Malaria is a major concern in Nigeria, and stands as the second leading cause of death from all infectious disease in Africa. Several studies have reported the damaging effect of the parasite to various body organs especially the liver. Reports over time has shown the benefits of various plants extracts in ethno medicine. However, not much have been done on the effects of some of these extracts in combined form on its hepato protective assessment in comparison with any known ACT based anti malaria. The focus of this study was to explore the hepato protective properties of ethanoic extract of Carica papaya Linn, AzadirachtaIndica, CymbopogonCitratusagainst ACT based antimalarial therapy on plasmodium berghei parasitized wistar rats. Phytochemical analysis of the extracts were done according to the method described by Treaseand Evans. Hepato protective assessment were done using the liver function tests and assay of the liver histology respectively. One hundred and ten 110 rats distributed into 11 groups, each group having 10rats were used for the experiment. Negative control received just feed and water, Positive control were induced with the malaria parasite and given feed and water only. The tests groups were induced with malaria, received feed and water and treated with 500mg kg, 250mg kg and 165mg kg doses of the extracts, both individually and in combined forms, as well as the standard ACT anti malaria. Phytochemical screening showed that the plant extracts possessed high concentration of Tannins, Flavonoids, Saponins and Alkaloids. Plasmodium berghei increased the activities of ALP, ASP and ALT when compared with the positive control group. This may be attributed to increase in functional capacity of the liver as a result of the presence of the infection for the tests groups. Treatment with the plant extracts decreased ALP and ALT levels significantly P 0.05 , as well as AST levels except for the Neem extract. Histological examination of the liver of test animals showed no extensive damage to the tissue by the individual extracts when compared to the negative control group. Nnyaha Anthonia E. | Igbokwe Ugochukwu V. | Okonkwo Onyeka Chukwudi | Ajeka Prisca O. | Nwaissac Ikechukwu S. | Okpa Precious N. "Hepato-Protective Assessment of Pawpaw Leaves, Neem, Lemon Grass and Acts on Plasmodium Berghei Parasitized Wistar Rats" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd46292.pdf Paper URL: https://www.ijtsrd.com/medicine/physiology/46292/hepatoprotective-assessment-of-pawpaw-leaves-neem-lemon-grass-and-acts-on-plasmodium-berghei-parasitized-wistar-rats/nnyaha-anthonia-e
Testing for COVID-19 with Antibody and PCR Tests in Low Resource Areas
One of the recommendations for mass testing developed by UPM and USA researchers backed by PAASE sent to DOH awaiting possible adoption
Meagan Hicks CV 2016
Meagan Hicks has a Bachelors of Science in Biochemistry from the University of New Brunswick. She has work experience researching Spartina grasses in salt marsh restoration and researching metabolism genetics in fruit flies. Her skills include experimental design, statistical analysis, bioinformatics, and laboratory techniques. She is proficient in French and has knowledge of German, Spanish, and Mandarin.
2014 SUCR Poster
1. The researchers collected frog egg masses from bodies of water in Illinois to isolate water mold pathogens.
2. They obtained DNA sequences from the ITS region of fourteen water mold isolates and classified the isolates using phylogenetic analysis.
3. The researchers designed a locked nucleic acid primer/probe set for quantitative PCR (qPCR) to identify a specific Saprolegnia strain. They are currently testing the probe's ability to only amplify the desired strain.
Similar to Prof Cannas Reference (14)
Biogenic antimicrobial silver nanoparticles produced by fungi
Biogenic antimicrobial silver nanoparticles produced by fungi
Hepato Protective Assessment of Pawpaw Leaves, Neem, Lemon Grass and Acts on ...
Hepato Protective Assessment of Pawpaw Leaves, Neem, Lemon Grass and Acts on ...
Testing for COVID-19 with Antibody and PCR Tests in Low Resource Areas
Testing for COVID-19 with Antibody and PCR Tests in Low Resource Areas
Prof Cannas Reference
- 1. UNIVERSITA’ DEGLI STUDI DI CAGLIARI DIPARTIMENTO SCIENZE DELLA VITA E DELL’AMBIENTE Via T. Fiorelli, 1 - 09126 CAGLIARI Cagliari, 18th November 2015 To whom it may concern, I am writing in support of my ex-student Michaela Agrifoglio, and strongly recommend her for your programme. Michaela Agrifoglio completed a Master Degree in Marine Bioecology at the University of Cagliari, during which she attended my laboratory for the internship devoted to the completion her experimental thesis. She demonstrated excellent skills and applied her knowledge. She is talented in molecular biology, such as DNA extraction from a specimen by using the salting-out technique (DNA Extraction), DNA barcoding, Polymerase Chain Reaction or PCR (a technique for in vitro synthesis of DNA), and gel electrophoresis. Her research comprised also an accurate bioinformatics analysis using the MEGA6, Arlequin (WinArl35.exe) and Dnasp programmes. The aim of her study was to acquire data for the conservation of endangered species or the proper management of commercial species. In particular she studied Dipturus oxyrinchus (longnose skate) to identify possible genetic variability among the populations in the Sea of Sardinia (Western Mediterranean Sea, Italy). During her time in my lab, Michaela Agrifoglio demonstrated good interpersonal skills. In conclusion, I definitely recommend Michaela Agrifoglio for your internship or PhD programme. I am glad I had the opportunity to be her teacher through to the final discussion of her project, and I am confident her knowledge will grow through your scientific programme. Please feel free to contact me for further information. Yours faithfully, Rita Cannas Associate Professor of Zoology University of Cagliari – Italy Department of Life and Environmental Sciences Section of Animal biology and Ecology Via T. Fiorelli, 1 09126 Cagliari (CA) Tel +39070/675-8004 fax E-mail: rcannas@unica.it