The document describes Shruthi Raghuraman's work using nano biotechnology for cancer detection and treatment, including creating hydrogel microparticles loaded with nanoparticles and a drug to target and slowly release medicine to tumor cells. The goals of the work were to locate cancer cells, carry nanoparticles to tumors, and slowly release medicine, and key parameters that were tested included alginate concentration, calcium chloride concentration, flow rate, agitation, and voltage. Shruthi acknowledges several people who assisted with her authentic science research project.

1. Nano Biotechnology in Disease
Detection and Therapy
Shruthi Raghuraman
Authentic Science Research
Senior – DHS
Note: Sharing my work here in a RESTRICTED
way as I am presenting my ASR work to a
nationally renowned Science competition.

2. Goals
1.Locate cancer cells
2.Carry nanoparticles to tumor
3.Slowly release medicine

3. Parameters
• Alginate Concentration
• Calcium Chloride Concentration
• Flow Rate
• Agitation
• Voltage

4. Procedure
• Target Size: • Target Size:
– 150 µL (diameter) – 100 µL (diameter)
– 450 µL (diameter) – 500 µL (diameter)
• Stable Shape • Stable shape
• Visibility • Visibility
• Uniformity • Uniformity

5. Micro-particles

6. Hydrogel Micro-encapsulator

8. Polystyrene Nanoparticles

9. In the Maestro

10. Loading Alginate gel with Nile Red
Microparticles

11. Things I Learned:
• Plastic Wrap
• Disinfect
• Research Programs
• Ask Questions

12. Acknowledgements
• Dr. Yong Wang - Dept of Chemical, Materials & Biomolecular Engineering
• Mark Battig - Research Graduate
• Shihui Li - Research Graduate
• Kelsey Boch - Undergraduate Student
• Xhaolong Zhang
• Niancao Chen
• Mr. Lewis - ASR - DHS
• Mrs. Leventhal - ASR - DHS