This study analyzed the occurrence and cellular immune responses of fish to Viral Hemorrhagic Septicemia virus (VHSv) in Lake Erie. Samples were collected from Lake Erie in 2016 and tested for VHSv. Positive samples were cultured and examined for cytopathic effects and compared to a 2003 VHSv isolate. Three 2016 isolates showed cytopathic effects at lower levels than the 2003 isolate. The virus is still present in Lake Erie.