Plant Metabolism

1. Set-upB
Mock test- 2025

2. Department of Botany
B.Sc. (H) Botany VI Sem
Plant Metabolism Practical Examination (May 2025)

3. Question -1
Perform the allotted experiment (A) and write
• Requirements- 3
• Theory/Principle -3
• Procedure-3
• Expected Observations-3
• Results and Graph -3
• Discussion-3
• Precautions-2
20 marks

4. List of experiment
1. Study the effect of substrate concentration on the activity of the enzyme
urease.
2. Study the effect of pH on the activity of enzyme catalase.
3. Separation photosynthetic pigments chemically.
4. Demonstrate Hill’s reaction.
5. Study the Blackman's law of limiting factors.
6. Compare the rate of respiration in different parts of a plant (at least three parts).
7. Study activity of Nitrate reductase in leaves of two plant sources.

5. • Q.2 Set up B
10 marks
• Q.3 Set up C
10 marks
• Q.4 Continuous Evaluation: 20 marks
• Class room performance /Test -10
• Lab Records 10

6. Set-up B
1. What does this set-up demonstrate?
2. What is the significance of this experiment?
3. What are the sources of error in this
experiment?
4. Why R.Q. of lipids is less than 1 and that of
organic acid is more than 1?
5. Write down two important precautions?
6. What are the criteria for selection of plant
material?
7. Why seeds are used to demonstrate this
experiment?
8. Why the value obtained by this experiment
for lipids is less than 1 and that of organic
acid is more than 1?
9. What will happen if (a) seeds are boiled (b)
dry seeds are used?
10. Explain physiological changes that occur in
the germinating seeds.
11. Comment on the substarte being respired of
the RQ value are (i) ∞ (ii) zero

7. Set-up B
12. Why majority of oil seeds are
smaller and prefer fats as storage
material?
13. Why R.Q. of lipids is less than 1
and that of organic acid is more than
1?
14. Which other chemical compound
can be used to absorb carbon
dioxide?
15. Write down the principle involved?
16. What will be the water level in the
pipette after one hour?
17.What will be the water level after
one hour if the material taken is
germinated mustard seeds
18. Write two important precautions
19.Why the green tissue is covered
with black paper?

8. Set up B
1. What does this set-up demonstrate?
2. What is the principle of this experiment?
3. In which form the seeds store the substrate used in
this experiment and Why?
4. Write the difference between a solution and emulsion.
5. What are emulsions and emulsifiers?
6.Write the catalyzing reaction of the enzyme?
7. Name two other plants which can be used in this
experiment.
8. Name the indicator used and what is the significance of
this indicator?
9. Why the oil seeds are small in size?
10. Name two tests for the presence of fats.

9. Set up C
1. What does this set up demonstrate?
2. What inference you draw from this graph.
3. What is the range of wavelength used to measure absorption spectrum of
chloroplast pigments?
4. In nature what is the significance of pigments having absorption maxima
at different wavelength.
5. Define absorption spectrum and action spectrum.
6. How is absorption spectrum of chlorophylls different from that of
carotenoids? What is the role of carotenoids
7. What is the range of wavelength used to measure absorption spectrum of
chloroplast pigments?
8. Name the instrument used to measure light absorption.
9. Name the instrument used to obtain such kind of graph.
10. What are photoreceptors?
11. Which colors of light are more useful in photosynthesis?
12. How is it different from action spectrum?
13. Which pigments are responsible for red and blue colouration in marine
algae?
14. Why chlorophyll a is called universal pigment?
15. Differentiate between chlorophyll a and chlorophyll b.

10. Set-up C
1. What does this set-up demonstrates?
2. Why does the two layers show the
difference in color?
3. What is the significance of this
phenomenon ?
4. Write the two main important
precautions to carry out this
experiment.
5. What is the principle of separation of
these two layers in this particular step?
6. What is the selective advantage of this
phenomenon for the plants ?
7. Name two examples where this
phenomenon can be observed.

11. Set-up C
8.Why do you think
chlorophyll are green in
colour?
9.Name the pigment which
absorbs the light of minimum
and maximum wavelength.
10. Why this phenomenon is
not observed in intact leaves?
11.Differentiate between
phosphorescence and
fluorescence.
12. Mention the conditions
leading fluorescence.