This document provides instructions for constructing a phylogenetic tree using sequence data in 5 steps: 1) paste sequences into a text file, 2) load the text file into Clustal X2 to create an alignment file, 3) open the alignment file in MEGA to convert it to a MEGA format file, 4) use MEGA to construct a phylogenetic tree using the neighbor-joining method on the nucleotide sequences in the MEGA format file, 5) the phylogenetic tree is now constructed.