AIM
• To findout the effect of given drug on locomotor activity of mice
using acto-photometer
• REQUIREMENTS
• Animal: Mice
• Drugs: CNS Depressant- Chlorpromazine, barbiturates and
alcohol
CNS Stimulant - Caffeine, Amphetamine and Modafinil
• Route of drug administration: intra peritoneal
3.
PRINCIPLE
• Locomotor activityor spontaneous motor activity is a horizontal activity of
animals and is one of the indices for the level of central nervous system
activity or mental alertness.
• CNS stimulant drugs increases and depressants lower the locomotor activity.
• This can be measured by using acto-photometer.
• Acto-photometer consists or either circular or square arena.
• Wall of the arena contains photocells that send beams of light to the arena and
these are connected to a counter.
• When animal interrupt the beam of the light that will be counted by the counter.
4.
IR Actimeter
• CompactInfrared (IR) Actimeter for the study of spontaneous locomotor
activity, rearings and optionally hole-board test parameters for exploration in
rodents.
• Minimum maintenance required
• Minimum lighting conditions required
• Interchangeable frames can be used without distinction for either REAR,
ACT or POKING modes
• Can be used with or without any computer (independent control units)
5.
PROCEDURE
• Weigh andnumber the animal and divide them in to three groups namely, control,
test 1 and test 2
• Administer saline to control group, to test chlorpromazine (3 mg/kg i.p.) or Caeffine
(20mg/kg i.p.)
• Thirty minutes after the treatment keep the animals individually in the acto-
photometer and record the loco-motor activity for five minutes.
• Calculate the mean± SEM for the data obtained in this experiment and find out
whether the drugs produce any change in the loco-motor activity using appropriate
statistical model
OBSERVATION
S.
No:
Body
weight
Treatme
nt
Locomotor activity score%
Inhibiti
on
Before After
SM FM SM FM
1 22 gm Control 18 62 15 65
2 25 gm Drug A 12 57 10 14
So What is the drug A? CNS Stimulant or Depressant
% Inhibition = Vc – Vt/Vt* 100
Vc – Control reading
Vt – Test Reading
