Person A has 10 mutations in the 20 bp region where the forward primer binds for amplifying D1S80. These mutations significantly change the DNA sequence where the forward primer binds. There will be no PCR product or a very low level of PCR product because the forward primer will not anneal or will anneal at a very low level due to the mutations. The reverse primer may still bind provided there are no mutations where it anneals.

1. Person A has 10 mutations in the 20 bp region where the forward primer binds during amplification
of D1S80. These mutations change the DNA sequence of that region significantly. Which of the
following is the likely outcome during PCR? (Select ail that apply) The reverse primer will bind
provided that there are no mutations in the region where it anneals. There will be no PCR product
or very low level of PCR product The forward primer will not anneal or will anneal at a very low
level There will be ample amount of PCR product but only from the reverse strandt