The document discusses the importance of histology in endoscopy for providing precise diagnoses of abnormalities and lesions seen during endoscopic examinations. It covers the basic processes involved in biopsy collection and preparation of samples for microscopic examination, including fixation, processing, sectioning, staining, and the use of immunohistochemistry and other ancillary techniques to aid in diagnosis. Close collaboration between endoscopists and pathologists is emphasized to ensure high quality samples and diagnostic yield.
This document provides information on the preparation and maintenance of museum specimens for pathology teaching. It discusses the key steps of reception, preparation, fixation, restoration, preservation and presentation of specimens. Fixation using Kaiserling technique is described to arrest autolysis and preserve tissue constituents. Specimen restoration in ethyl alcohol and rejuvenator solution helps maintain color. Special techniques for different organs and tissues like brain, heart and bone are also outlined. Proper labeling and cataloguing of specimens is important for accessibility.
Fluid control and soft tissue managementDr.Amrit Assi
This document discusses various methods for controlling fluids and soft tissues during restorative dental procedures. It describes the importance of fluid control for patient comfort, operator safety and access. Rubber dams, high-volume vacuums and saliva ejectors are discussed as fluid control methods. Methods for displacing gingival tissues are also covered, including the use of displacement cords, which can be impregnated with chemicals to aid retraction when placed in the sulcus. Proper technique for cord placement is described to minimize risk of damage to gingival tissues.
It shows methods of gingival retraction and its recent advances.
gingival retraction is done prion to tooth preparation or impression making to widen the gingival sulcus for easy access to the margin around tooth that is prepared.
This document provides information about different types of biopsies. It defines a biopsy as a procedure to remove tissue or cells from the body for laboratory analysis. The main types discussed are excisional biopsy, incisional biopsy, punch biopsy, exfoliative cytology, aspiration biopsy and brush biopsy. Indications, procedures, advantages and disadvantages are described for each type. Potential dangers of biopsies mentioned include spreading infection, hemorrhage, infection, operative trauma and wounding of cancer tissue. The document serves to inform about various biopsy procedures.
This document provides information on the management of burn injuries. It discusses initial first aid including stopping the burning process and cooling the burned area. It then covers indications for admission, initial treatment including cleaning and dressing, fluid resuscitation protocols, wound management including dressing, grafting and escharotomy, and prevention and treatment of contractures. The document is a comprehensive overview of burn management from initial emergency response through long-term wound healing and rehabilitation.
This document discusses various types and mechanisms of filtration. It begins by defining filtration as the process of separating solids from fluids by passing them through a porous medium. Some key applications include producing sterile drugs and liquid oral formulations. The main types of filtration are surface, depth, and cake filtration. Surface filtration relies on pore size to retain solids, while depth filtration relies on particle entanglement within pores. Cake filtration uses a woven cloth to form a particle bed. Factors like particle size and pressure influence the filtration rate. Common equipment includes plate and frame presses and filter leaves.
This document outlines the roles and responsibilities of various members of the surgical team. It discusses preoperative, intraoperative, and postoperative care processes. The key members of the surgical team include the surgeon, anesthesiologist, certified registered nurse anesthetist, circulating nurse, and scrub nurse. Their roles involve ensuring patient safety and performing tasks like administering anesthesia, setting up the operating room, assisting with the procedure, and maintaining sterility.
This document provides information on the preparation and maintenance of museum specimens for pathology teaching. It discusses the key steps of reception, preparation, fixation, restoration, preservation and presentation of specimens. Fixation using Kaiserling technique is described to arrest autolysis and preserve tissue constituents. Specimen restoration in ethyl alcohol and rejuvenator solution helps maintain color. Special techniques for different organs and tissues like brain, heart and bone are also outlined. Proper labeling and cataloguing of specimens is important for accessibility.
Fluid control and soft tissue managementDr.Amrit Assi
This document discusses various methods for controlling fluids and soft tissues during restorative dental procedures. It describes the importance of fluid control for patient comfort, operator safety and access. Rubber dams, high-volume vacuums and saliva ejectors are discussed as fluid control methods. Methods for displacing gingival tissues are also covered, including the use of displacement cords, which can be impregnated with chemicals to aid retraction when placed in the sulcus. Proper technique for cord placement is described to minimize risk of damage to gingival tissues.
It shows methods of gingival retraction and its recent advances.
gingival retraction is done prion to tooth preparation or impression making to widen the gingival sulcus for easy access to the margin around tooth that is prepared.
This document provides information about different types of biopsies. It defines a biopsy as a procedure to remove tissue or cells from the body for laboratory analysis. The main types discussed are excisional biopsy, incisional biopsy, punch biopsy, exfoliative cytology, aspiration biopsy and brush biopsy. Indications, procedures, advantages and disadvantages are described for each type. Potential dangers of biopsies mentioned include spreading infection, hemorrhage, infection, operative trauma and wounding of cancer tissue. The document serves to inform about various biopsy procedures.
This document provides information on the management of burn injuries. It discusses initial first aid including stopping the burning process and cooling the burned area. It then covers indications for admission, initial treatment including cleaning and dressing, fluid resuscitation protocols, wound management including dressing, grafting and escharotomy, and prevention and treatment of contractures. The document is a comprehensive overview of burn management from initial emergency response through long-term wound healing and rehabilitation.
This document discusses various types and mechanisms of filtration. It begins by defining filtration as the process of separating solids from fluids by passing them through a porous medium. Some key applications include producing sterile drugs and liquid oral formulations. The main types of filtration are surface, depth, and cake filtration. Surface filtration relies on pore size to retain solids, while depth filtration relies on particle entanglement within pores. Cake filtration uses a woven cloth to form a particle bed. Factors like particle size and pressure influence the filtration rate. Common equipment includes plate and frame presses and filter leaves.
This document outlines the roles and responsibilities of various members of the surgical team. It discusses preoperative, intraoperative, and postoperative care processes. The key members of the surgical team include the surgeon, anesthesiologist, certified registered nurse anesthetist, circulating nurse, and scrub nurse. Their roles involve ensuring patient safety and performing tasks like administering anesthesia, setting up the operating room, assisting with the procedure, and maintaining sterility.
This document discusses anaesthesia considerations for robotic surgery. Key points include:
1) Robotic surgery allows for improved precision, control and visualization compared to laparoscopic surgery.
2) Positioning is critical as the patient cannot be repositioned once the robot is docked. Extremes in positioning can impact physiology.
3) Physiologic perturbations like increased CO2 absorption and changes to ventilation and oxygenation during thoracic procedures must be managed.
4) Access to the patient can be challenging with the robot in place, especially for airway management or procedures requiring fiberoptic bronchoscopy.
This document discusses anaesthesia considerations for robotic surgery. Key points include:
- Robotic surgery allows for improved precision, control and visualization compared to laparoscopic surgery.
- Patient positioning is critical and cannot be adjusted once the robot is docked, so optimal positioning is important.
- Physiologic perturbations like increased CO2 absorption and changes to ventilation and perfusion during pneumoperitoneum or one-lung ventilation must be managed.
- Access to the patient can be challenging with the robot in place, so airway management and monitoring require planning.
This document discusses anterior strip crowns, which are full coverage restorations for primary anterior teeth with large cavities. It defines strip crowns, outlines their purpose and indications, contraindications, placement technique, advantages and disadvantages. Strip crowns provide aesthetic restoration while protecting teeth from further decay, but they can fracture or debond and require careful technique and moisture control. Overall, strip crowns are considered an effective esthetic option for restoring severely decayed primary incisors when more conservative treatments are not sufficient.
The document discusses the treatment of cervical dysplasias and cervical intraepithelial neoplasia (CIN). It states that treatment based solely on cytology or colposcopy findings can lead to incorrect diagnoses. For mild dysplasia/CIN1, follow up is usually sufficient as it often resolves on its own. For moderate to severe dysplasias (CIN2/3), local destructive methods like cryotherapy or excisional methods like LEEP are recommended. Conservative treatments are only advised if the entire lesion is visible and there is no invasion. The document also discusses vaccination as a preventive measure against HPV, which causes most cervical cancers.
Endodontic mishaps/PROCEDURAL ACCIDENTSNivedha Tina
The document discusses various endodontic mishaps that can occur during root canal treatment such as loss of working length, canal blockages, ledging, missed canals, instrument separation, perforations, and over/under filling. It describes the causes, signs, and management of each mishap. Prevention is key and includes proper access cavity preparation, using sequential file sizes with copious irrigation, verifying instrument position radiographically, and maintaining sterile conditions with a rubber dam. The document emphasizes informing patients about any procedural accidents and their treatment and prognosis implications.
Contact lenses can be prescribed for elderly patients to correct vision and rehabilitate the cornea, but their success is often limited by age-related physiological changes like dry eye, decreased manual dexterity, and high refractive error. A thorough assessment of anatomical, refractive, corneal, tear film, lid, and visual factors is important prior to fitting to increase the likelihood of success. Specialty lenses, careful handling, and family support may help elderly patients wear contacts.
This document provides guidelines for the management of sepsis from 2016 and 2018 updates. It begins with discussing the historical perspectives on defining and treating sepsis. Key points include:
- Sepsis and septic shock definitions were revised in 2016 to focus on life-threatening organ dysfunction caused by a dysregulated immune response to infection.
- Screening tools like SOFA and qSOFA are recommended to help identify and monitor sepsis severity. The SOFA score evaluates organ dysfunction in six organ systems while qSOFA is for non-ICU patients.
- The initial management of sepsis focuses on rapid treatment within the first hour of recognition, including measuring lactate, blood cultures, antibiotics, fluid resuscitation, and
This document provides information on penetrating keratoplasty (PKP), which involves replacing the full thickness of diseased corneal tissue with donor corneal tissue. It discusses the types of PKP including optical, therapeutic, and tectonic. The common indications for PKP include corneal scarring, infections, dystrophies, and thinning. Preoperative evaluation and obtaining a suitable donor corneal tissue is important. The surgical procedure involves trephination of the donor and host corneas followed by suturing the donor graft. Postoperative complications can include rejection, infections, glaucoma and astigmatism. Long term graft survival depends on the preoperative diagnosis and condition.
- Posterior polar cataracts are congenital cataracts caused by persistence of the hyaloid artery during embryonic development.
- They present as dense, circular opacities in the central posterior capsule and are associated with a high risk of posterior capsule rupture during surgery due to the thinness and fragility of the posterior capsule.
- Surgical techniques for posterior polar cataracts emphasize gentle phacoemulsification and removal of the lens material in thin layers to minimize risks of posterior capsule rupture. Removing the posterior polar opacity last and using viscodissection techniques may also help reduce surgical complications.
This document discusses various surgical procedures for glaucoma management. It provides details on laser trabeculoplasty, incisional surgery such as filtering procedures, laser iridotomy, glaucoma drainage devices, and cyclodestructive procedures. Laser trabeculoplasty involves applying laser energy to the trabecular meshwork to lower intraocular pressure. Filtering surgery involves creating a fistula to allow aqueous humor to drain from the anterior chamber. Laser iridotomy is used to treat pupillary block glaucoma by creating an iridotomy. Glaucoma drainage devices provide an alternative drainage pathway through a tube. Cyclodestructive procedures aim to reduce aqueous production by ablating the ciliary body. The
The document provides information about the Active Cycle of Breathing Techniques (ACBT), which is used to mobilize and clear excess pulmonary secretions. It involves three phases - breathing control, thoracic expansion, and forced expiratory technique (FET) - repeated in cycles. The techniques can help loosen and clear secretions from the lungs, improve ventilation, and strengthen cough effectiveness. ACBT encourages active patient participation and has been shown to be effective when performed independently. Precautions must be taken for patients with conditions like hyperreactive airways or recent surgery.
The document describes the six minute walk test (6MWT), which measures the distance that a patient can quickly walk over a total of 6 minutes. The 6MWT evaluates a patient's overall functional exercise capacity and is useful for assessing patients with cardiopulmonary diseases before and after treatment. It is a self-paced test that requires only a 100ft hallway and does not use complex equipment. The document outlines how to properly perform and score the 6MWT according to standardized procedures.
Anorectal Malformation for BSc Nursing/PB BSc Nursinggautamicharingia
Anorectal Malformation, in which you will learn about its types, incidence, causes, risk factors, signs and symptoms, associated abnormalities, diagnostic evaluation, surgical and nursing management. It also includes anal dilation, colostomy care and family education.
This document provides instructions for extracting, purifying, storing, and analyzing genomic bacterial DNA from clinical isolates and specimens. It describes efficient methods for lysing bacterial cells using chemical or enzymatic treatments. DNA is then purified using phenol/chloroform extraction and ethanol precipitation to remove cell debris and proteins. Purified DNA can be stored at -20°C or -80°C for long term storage. Analysis of PCR products is done by running samples on an agarose gel and visualizing under UV light to identify bands corresponding to bacterial species and serotypes.
Routine histopathology techniques and staining [Autosaved].pptxchandreshmishra13
The document provides information about routine histopathology techniques and staining. It discusses fixation of histology samples, ideal fixatives, changes after fixation, types of fixatives, and the mechanisms of fixation. It also covers tissue processing techniques including dehydration, clearing, infiltration, embedding, and sectioning of tissue blocks using a microtome. Key steps in processing like fixation, dehydration, clearing and infiltration are described in detail. Common fixatives, dehydrating agents, and clearing agents used are also listed.
Routine histopathology techniques and staining [Autosaved].pptxchandreshmishra13
This document provides an overview of routine histopathology techniques and staining. It discusses fixation of histology samples using an ideal fixative like formaldehyde. It describes the changes that occur in tissue after fixation, including shrinkage and hardening. Different types of fixatives and their mechanisms of action are explained. The document also covers tissue processing techniques including dehydration, clearing, infiltration and embedding in paraffin wax. Key steps and factors influencing tissue processing are summarized. Staining, troubleshooting, decalcification and special techniques like tissue marking and orientation are briefly outlined.
Routine histopathology techniques and staining.pptxchandreshmishra13
The document provides information about routine histopathology techniques and staining. It discusses fixation of histology samples, ideal fixatives, changes after fixation, types of fixatives, and the mechanisms of fixation. It also covers tissue processing techniques including dehydration, clearing, infiltration, embedding, and sectioning of tissue blocks using a microtome. Key steps in processing like fixation, dehydration, clearing and infiltration are described in detail. Common fixatives, dehydrating agents, and clearing agents used are also listed.
This document discusses anaesthesia considerations for robotic surgery. Key points include:
1) Robotic surgery allows for improved precision, control and visualization compared to laparoscopic surgery.
2) Positioning is critical as the patient cannot be repositioned once the robot is docked. Extremes in positioning can impact physiology.
3) Physiologic perturbations like increased CO2 absorption and changes to ventilation and oxygenation during thoracic procedures must be managed.
4) Access to the patient can be challenging with the robot in place, especially for airway management or procedures requiring fiberoptic bronchoscopy.
This document discusses anaesthesia considerations for robotic surgery. Key points include:
- Robotic surgery allows for improved precision, control and visualization compared to laparoscopic surgery.
- Patient positioning is critical and cannot be adjusted once the robot is docked, so optimal positioning is important.
- Physiologic perturbations like increased CO2 absorption and changes to ventilation and perfusion during pneumoperitoneum or one-lung ventilation must be managed.
- Access to the patient can be challenging with the robot in place, so airway management and monitoring require planning.
This document discusses anterior strip crowns, which are full coverage restorations for primary anterior teeth with large cavities. It defines strip crowns, outlines their purpose and indications, contraindications, placement technique, advantages and disadvantages. Strip crowns provide aesthetic restoration while protecting teeth from further decay, but they can fracture or debond and require careful technique and moisture control. Overall, strip crowns are considered an effective esthetic option for restoring severely decayed primary incisors when more conservative treatments are not sufficient.
The document discusses the treatment of cervical dysplasias and cervical intraepithelial neoplasia (CIN). It states that treatment based solely on cytology or colposcopy findings can lead to incorrect diagnoses. For mild dysplasia/CIN1, follow up is usually sufficient as it often resolves on its own. For moderate to severe dysplasias (CIN2/3), local destructive methods like cryotherapy or excisional methods like LEEP are recommended. Conservative treatments are only advised if the entire lesion is visible and there is no invasion. The document also discusses vaccination as a preventive measure against HPV, which causes most cervical cancers.
Endodontic mishaps/PROCEDURAL ACCIDENTSNivedha Tina
The document discusses various endodontic mishaps that can occur during root canal treatment such as loss of working length, canal blockages, ledging, missed canals, instrument separation, perforations, and over/under filling. It describes the causes, signs, and management of each mishap. Prevention is key and includes proper access cavity preparation, using sequential file sizes with copious irrigation, verifying instrument position radiographically, and maintaining sterile conditions with a rubber dam. The document emphasizes informing patients about any procedural accidents and their treatment and prognosis implications.
Contact lenses can be prescribed for elderly patients to correct vision and rehabilitate the cornea, but their success is often limited by age-related physiological changes like dry eye, decreased manual dexterity, and high refractive error. A thorough assessment of anatomical, refractive, corneal, tear film, lid, and visual factors is important prior to fitting to increase the likelihood of success. Specialty lenses, careful handling, and family support may help elderly patients wear contacts.
This document provides guidelines for the management of sepsis from 2016 and 2018 updates. It begins with discussing the historical perspectives on defining and treating sepsis. Key points include:
- Sepsis and septic shock definitions were revised in 2016 to focus on life-threatening organ dysfunction caused by a dysregulated immune response to infection.
- Screening tools like SOFA and qSOFA are recommended to help identify and monitor sepsis severity. The SOFA score evaluates organ dysfunction in six organ systems while qSOFA is for non-ICU patients.
- The initial management of sepsis focuses on rapid treatment within the first hour of recognition, including measuring lactate, blood cultures, antibiotics, fluid resuscitation, and
This document provides information on penetrating keratoplasty (PKP), which involves replacing the full thickness of diseased corneal tissue with donor corneal tissue. It discusses the types of PKP including optical, therapeutic, and tectonic. The common indications for PKP include corneal scarring, infections, dystrophies, and thinning. Preoperative evaluation and obtaining a suitable donor corneal tissue is important. The surgical procedure involves trephination of the donor and host corneas followed by suturing the donor graft. Postoperative complications can include rejection, infections, glaucoma and astigmatism. Long term graft survival depends on the preoperative diagnosis and condition.
- Posterior polar cataracts are congenital cataracts caused by persistence of the hyaloid artery during embryonic development.
- They present as dense, circular opacities in the central posterior capsule and are associated with a high risk of posterior capsule rupture during surgery due to the thinness and fragility of the posterior capsule.
- Surgical techniques for posterior polar cataracts emphasize gentle phacoemulsification and removal of the lens material in thin layers to minimize risks of posterior capsule rupture. Removing the posterior polar opacity last and using viscodissection techniques may also help reduce surgical complications.
This document discusses various surgical procedures for glaucoma management. It provides details on laser trabeculoplasty, incisional surgery such as filtering procedures, laser iridotomy, glaucoma drainage devices, and cyclodestructive procedures. Laser trabeculoplasty involves applying laser energy to the trabecular meshwork to lower intraocular pressure. Filtering surgery involves creating a fistula to allow aqueous humor to drain from the anterior chamber. Laser iridotomy is used to treat pupillary block glaucoma by creating an iridotomy. Glaucoma drainage devices provide an alternative drainage pathway through a tube. Cyclodestructive procedures aim to reduce aqueous production by ablating the ciliary body. The
The document provides information about the Active Cycle of Breathing Techniques (ACBT), which is used to mobilize and clear excess pulmonary secretions. It involves three phases - breathing control, thoracic expansion, and forced expiratory technique (FET) - repeated in cycles. The techniques can help loosen and clear secretions from the lungs, improve ventilation, and strengthen cough effectiveness. ACBT encourages active patient participation and has been shown to be effective when performed independently. Precautions must be taken for patients with conditions like hyperreactive airways or recent surgery.
The document describes the six minute walk test (6MWT), which measures the distance that a patient can quickly walk over a total of 6 minutes. The 6MWT evaluates a patient's overall functional exercise capacity and is useful for assessing patients with cardiopulmonary diseases before and after treatment. It is a self-paced test that requires only a 100ft hallway and does not use complex equipment. The document outlines how to properly perform and score the 6MWT according to standardized procedures.
Anorectal Malformation for BSc Nursing/PB BSc Nursinggautamicharingia
Anorectal Malformation, in which you will learn about its types, incidence, causes, risk factors, signs and symptoms, associated abnormalities, diagnostic evaluation, surgical and nursing management. It also includes anal dilation, colostomy care and family education.
This document provides instructions for extracting, purifying, storing, and analyzing genomic bacterial DNA from clinical isolates and specimens. It describes efficient methods for lysing bacterial cells using chemical or enzymatic treatments. DNA is then purified using phenol/chloroform extraction and ethanol precipitation to remove cell debris and proteins. Purified DNA can be stored at -20°C or -80°C for long term storage. Analysis of PCR products is done by running samples on an agarose gel and visualizing under UV light to identify bands corresponding to bacterial species and serotypes.
Routine histopathology techniques and staining [Autosaved].pptxchandreshmishra13
The document provides information about routine histopathology techniques and staining. It discusses fixation of histology samples, ideal fixatives, changes after fixation, types of fixatives, and the mechanisms of fixation. It also covers tissue processing techniques including dehydration, clearing, infiltration, embedding, and sectioning of tissue blocks using a microtome. Key steps in processing like fixation, dehydration, clearing and infiltration are described in detail. Common fixatives, dehydrating agents, and clearing agents used are also listed.
Routine histopathology techniques and staining [Autosaved].pptxchandreshmishra13
This document provides an overview of routine histopathology techniques and staining. It discusses fixation of histology samples using an ideal fixative like formaldehyde. It describes the changes that occur in tissue after fixation, including shrinkage and hardening. Different types of fixatives and their mechanisms of action are explained. The document also covers tissue processing techniques including dehydration, clearing, infiltration and embedding in paraffin wax. Key steps and factors influencing tissue processing are summarized. Staining, troubleshooting, decalcification and special techniques like tissue marking and orientation are briefly outlined.
Routine histopathology techniques and staining.pptxchandreshmishra13
The document provides information about routine histopathology techniques and staining. It discusses fixation of histology samples, ideal fixatives, changes after fixation, types of fixatives, and the mechanisms of fixation. It also covers tissue processing techniques including dehydration, clearing, infiltration, embedding, and sectioning of tissue blocks using a microtome. Key steps in processing like fixation, dehydration, clearing and infiltration are described in detail. Common fixatives, dehydrating agents, and clearing agents used are also listed.
Dr. Nirupama Kothari runs a histopathology lab located in Jodhpur, India. The lab has 2 histopathologists, with one working full time and one part time. They process samples in-house and receive approximately 600-650 biopsy samples, 200-225 cytology samples, and 20-22 IHC samples per month. Their workload consists mainly of gastrointestinal, genitourinary, and breast biopsy samples, with 15% being oncology cases. They have 2 technical staff and classify samples as large biopsies, medium biopsies, small biopsies, and various types of cytology samples. Their areas of specialty include oncopathology, hematopathology, and
Cervical cancer screening using cytology has significantly reduced cervical cancer mortality by detecting precancerous lesions. Screening is most effective in women aged 25-29 years old, as this is when high-grade lesions are most commonly detected. The Bethesda system provides standardized terminology for cervical cytology reports and categorizes results as negative, atypical, low-grade or high-grade abnormalities. Co-testing with HPV testing and cytology is superior to cytology alone and has been adopted in updated cervical cancer screening guidelines.
At Apollo Hospital, Lucknow, U.P., we provide specialized care for children experiencing dehydration and other symptoms. We also offer NICU & PICU Ambulance Facility Services. Consult our expert today for the best pediatric emergency care.
For More Details:
Map: https://cutt.ly/BwCeflYo
Name: Apollo Hospital
Address: Singar Nagar, LDA Colony, Lucknow, Uttar Pradesh 226012
Phone: 08429021957
Opening Hours: 24X7
Exploring the Benefits of Binaural Hearing: Why Two Hearing Aids Are Better T...Ear Solutions (ESPL)
Binaural hearing using two hearing aids instead of one offers numerous advantages, including improved sound localization, enhanced sound quality, better speech understanding in noise, reduced listening effort, and greater overall satisfaction. By leveraging the brain’s natural ability to process sound from both ears, binaural hearing aids provide a more balanced, clear, and comfortable hearing experience. If you or a loved one is considering hearing aids, consult with a hearing care professional at Ear Solutions hearing aid clinic in Mumbai to explore the benefits of binaural hearing and determine the best solution for your hearing needs. Embracing binaural hearing can lead to a richer, more engaging auditory experience and significantly improve your quality of life.
The facial nerve, also known as cranial nerve VII, is one of the 12 cranial nerves originating from the brain. It's a mixed nerve, meaning it contains both sensory and motor fibres, and it plays a crucial role in controlling various facial muscles, as well as conveying sensory information from the taste buds on the anterior two-thirds of the tongue.
Can coffee help me lose weight? Yes, 25,422 users in the USA use it for that ...nirahealhty
The South Beach Coffee Java Diet is a variation of the popular South Beach Diet, which was developed by cardiologist Dr. Arthur Agatston. The original South Beach Diet focuses on consuming lean proteins, healthy fats, and low-glycemic index carbohydrates. The South Beach Coffee Java Diet adds the element of coffee, specifically caffeine, to enhance weight loss and improve energy levels.
Can Allopathy and Homeopathy Be Used Together in India.pdfDharma Homoeopathy
This article explores the potential for combining allopathy and homeopathy in India, examining the benefits, challenges, and the emerging field of integrative medicine.
About this webinar: This talk will introduce what cancer rehabilitation is, where it fits into the cancer trajectory, and who can benefit from it. In addition, the current landscape of cancer rehabilitation in Canada will be discussed and the need for advocacy to increase access to this essential component of cancer care.
Hypertension and it's role of physiotherapy in it.Vishal kr Thakur
This particular slides consist of- what is hypertension,what are it's causes and it's effect on body, risk factors, symptoms,complications, diagnosis and role of physiotherapy in it.
This slide is very helpful for physiotherapy students and also for other medical and healthcare students.
Here is summary of hypertension -
Hypertension, also known as high blood pressure, is a serious medical condition that occurs when blood pressure in the body's arteries is consistently too high. Blood pressure is the force of blood pushing against the walls of blood vessels as the heart pumps it. Hypertension can increase the risk of heart disease, brain disease, kidney disease, and premature death.
We are one of the top Massage Spa Ajman Our highly skilled, experienced, and certified massage therapists from different corners of the world are committed to serving you with a soothing and relaxing experience. Luxuriate yourself at our spas in Sharjah and Ajman, which are indeed enriched with an ambiance of relaxation and tranquility. We could confidently claim that we are one of the most affordable Spa Ajman and Sharjah as well, where you can book the massage session of your choice for just 99 AED at any time as we are open 24 hours a day, 7 days a week.
Visit : https://massagespaajman.com/
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Chandrima Spa Ajman is one of the leading Massage Center in Ajman, which is open 24 hours exclusively for men. Being one of the most affordable Spa in Ajman, we offer Body to Body massage, Kerala Massage, Malayali Massage, Indian Massage, Pakistani Massage Russian massage, Thai massage, Swedish massage, Hot Stone Massage, Deep Tissue Massage, and many more. Indulge in the ultimate massage experience and book your appointment today. We are confident that you will leave our Massage spa feeling refreshed, rejuvenated, and ready to take on the world.
Visit : https://massagespaajman.com/
Call : 052 987 1315
TEST BANK For Accounting Information Systems, 3rd Edition by Vernon Richardso...rightmanforbloodline
TEST BANK For Accounting Information Systems, 3rd Edition by Vernon Richardson, Verified Chapters 1 - 18, Complete Newest Version
TEST BANK For Accounting Information Systems, 3rd Edition by Vernon Richardson, Verified Chapters 1 - 18, Complete Newest Version
TEST BANK For Accounting Information Systems, 3rd Edition by Vernon Richardson, Verified Chapters 1 - 18, Complete Newest Version
Gemma Wean- Nutritional solution for Artemiasmuskaan0008
GEMMA Wean is a high end larval co-feeding and weaning diet aimed at Artemia optimisation and is fortified with a high level of proteins and phospholipids. GEMMA Wean provides the early weaned juveniles with dedicated fish nutrition and is an ideal follow on from GEMMA Micro or Artemia.
GEMMA Wean has an optimised nutritional balance and physical quality so that it flows more freely and spreads readily on the water surface. The balance of phospholipid classes to- gether with the production technology based on a low temperature extrusion process improve the physical aspect of the pellets while still retaining the high phospholipid content.
GEMMA Wean is available in 0.1mm, 0.2mm and 0.3mm. There is also a 0.5mm micro-pellet, GEMMA Wean Diamond, which covers the early nursery stage from post-weaning to pre-growing.
6. ENDOSCOPY AND HISTOLOGY
• DIRECT EXAMINATION OF ORGANS WITH OPTICAL METHODS.
• DETECT ABNORMALITIES OF NORMAL ANATOMY AND HISTOLOGY TO PROVIDE A PRECISE DIAGNOSIS.
• CLASSICAL ENDOSCOPY--- NAKED EYE OBSERVATION
• HISTOLOGY IS REACHES CELLULAR AND SUBCELLULAR LEVEL
• MAJOR CONTRIBUTION OF HISTOLOGY TO ENDOSCOPY IN INFLAMMATORY AND NEOPLASTIC DISEASES.
• TYPE& ETIOLOGY OF INFLAMMATION AND CLASSIFICATION ,IMPACT ON TREATMENT,
• FOR eg: GLUTEN SENSITIVE ENTEROPATHY,IDENTIFYING SPECIFIC PATHOGENS LIKE GIARDIA,MYCOBACTRIUM,CRYTOPSPORIDIA,AMOEBAE
• DETERMINING ETIOLOGY OF INFLAMMATION AS AUTOIMMUNE GASTRITIS, OR TYPING TUMORS(ADENOCA OR
LYMPHOMA)
• ELEMENTS FOR FURTHER TREATMENT STRATEGY BY PRESENCE OR ABSENCE OF RISK FACTORS
• LIKE RESIDUAL TUMOR IN POLYPECTOMY SPECIMEN OR ENDOSCOPIC MUCOSAL RESECTION,DEMONSTRATIONS OF
MUTATIONS LIKE KRAS IN CRC OR HER2 AMPLIFICATION IN ESOPHAGEAL AND GASTRIC CA,USE OF BIOMARKERS.
• THESE APPLICATIONS HAVE IMPORTANT THERAPEUTIC IMPLICATIONS
• KRAS GENE ASSOCIATED WITH POOR RESPONSE,HIGH HER2 EXPRESSION BENEFITTED BY TRASTUZUMAB
7. INFLUENCE OF ENDOSCOPY ON DIAGNOSTIC
YEILD OF HISTOLOGY
• GENERAL REQUIREMENTS
• CLOSE COLLABORATION BETWEEN ENDOSCOPIST AND PATHOLOGIST
• COPY OF ENDOSCOPY REPORT MENTIONING ;
• SITE OF BIOPSY, MACROSCOPIC DESCREPTION OF LESION AND ADJACENT MUCOSA , AGE, IMMUNE STATUS,
DURATION OF SYMPTOMS AND TREATMENT IF ANY.
• PATHOLOGIST SHOULD MENTION NUMBER,SIZE AND DEPTH OF SPECIMEN,
• PROBABILITY OF INITIAL DIAGNOSIS,SUGGEST FURTHER NEED FOR SAMPLING,IHC.
9. ENDOSCOPIC ULTRASOUND GUIDED FNAB.
• MOST ACCURATE MODALITY FOR CHARACTERIZATION OF PANCREATIC CYSTIC AND SOLID LESIONS, D/D
INTERMINATE MASSES AND LOCO-REGIONAL STAGGING OF SOME DIGESTIVE CANCERS.
• CAN BE PERFORMED IN THE PRIMARY MASS, DISTANT LYMPHNODES OR METASTATIC LOCATION.
• HIGH SENSITIVITY, SPECIFICITY, POSITIVE PREDECTIVE VALUE AND ACCURACY IN THE ASSESMENT OF
BILIOPANCREATIC TUMOR (DEPEND UPON THE EXPIRIENCE) OF CLINICIAN AND CYTOPATHOLOGIST.
• NUMBER OF SAMPLES:- DIAGNOSTIC YEILD OF HISTOPATHOLOGY IS INCREASED AND SAMPLING ERROR IS
DECREASED BY INCREASING THE NUMBER OF BIOPSIES.
• DIFFERENT GUIDELINES FOR ENDOSCOPIC SAMPLING IN VARIOUS DISEASES HAVE BEEN DEVELOPED.
• INTRODUCTION OF NEW TECHNOLOGY AND MODERN ENDOSCOPE WILL CHANGE PRACTICE IN FUTURE BY
OFFERING TARGETED BIOPSIES.
10. HOW TO SUBMIT SPECIMENS FOR HISTOPATHOLOGY.
• REQUISITION FORM / SUBMISSION FORM.
• CONTAINER.
• FIXATIVE.
• PROVIDE ANATOMICAL SITE, LESION DESCRIPTION AND PERTINENT CLINICAL INFORMATION ON THE
SUBMISSION FORM.
• IF YOU HAVE A LIST OF DIFFERENTIALS YOU’D LIKE TO RULE OUT, PLEASE MENTION SUCH.
• FIRST OF ALL AND MOST IMPORTANTLY CLINICIAN SHOULD TAKEN ADEQUATE CARE TO AVOID
CONTAMINATION OF TISSUE WITH TISSUE FROM OTHER PATEINT.
• THIS MAY HAPPEN IN OPERATION ROOM, CLINIC, OR IN PATHOLOGY LAB.
11.
12. SAMPLING CONTINUED
• A COMPLETE DIAGNOSIS INVOLVES;
TUMOR DIFFERENTIATION, SIZE, DETERMINATION OF DEEP
INFILTRATION, LYMPHATIC PERMEATION AND DETERMINATION OF
MARGIN (IDENTIFICATION OF THIS AREA IS EASY IF LESION IS
ADEQUATELY ORIENTED).
• POLYPECTOMY- ENDOSCOPIST SHOULD IDENTIFY
SECTION MARGINS WITH INDIA INK / PIN.
• EMR / ESD SPECIMEN SHOULD BE ORIENTED
PROPERLY
• PAINTING OF BASE AND MARGIN IS USEFUL.
13. SPECIMEN CONTAINER.
• PLASTIC OR GLASS JAR.
• LABEL MATCHING REQUISITION SLIP.
• REGISTERATION NUMBER.
• FULL NAME.
• AGE / SEX
• WARD NUMBER, & BED NUMBER.
• SITE AND SIDE.
• MORE SPECIMEN MARK AS A,B,C,D ETC.
• SIGNATION OF DOCTOR WITH DATE.
14. SAMPLING
• DIAGNOSTIC YEILD DEPENDS UPON EXPERIENCE ,QUALITY OF BIOPSY.
• PROBABILITY OF INITIAL DIAGNOSIS, TYPE OF BIOPSY, SIZE OF BIOPSY FORCEPS.
• ANATOMIC LOCATION OF CERTAIN LESION ARE OF LESS OF GOOD QUALITY OR
SUPERFICIAL FOR EXAMPLE AREAS IMMEDIATELY DISTAL TO A STRICTURE, PAPILLA OF
VATER, PANCREATIC DUCTS ETC.
• TO OBTAIN SAMPLES OF APROPIATE DEPTH, AIR IN INSUFFLATION DURING THE
ENDOSCOPY SHOULD BE THE LIMITED MUCOSA IS STRETCHED AND PUSHED TOWARDS
SUBMUCOSA AND THE SAMPLES ARE LIKELY TO BE THE SUPERFICIAL.
• USE OF ‘BURROWING’ TECHNIQUE- SEVERAL BIOPSY ARE TAKEN IN THE SAME AREA GIVING
INFLAMMATION DEEPLY SITUATED LESION.
• EUG FNA IS AN ALTERNATIVE; PERMITING MORPHOLOGIC AND CYTOLOGIC ANALYSIS OF LESIONS
WITHIN OR ADJACENT TO GIT.
• LARGER SAMPLES OBTAINED WITH EMR / ESD / SNARE POLYPECTOMY.
• PROPER HANDLING AND INTERPRETATION OF THIS SAMPLES HELP SUBSEQUENT MANAGEMENT.
15. SPECIMEN IDENTIFICATION AND LABELLING
• ANY DISCREPANCIES OF SPECIEN IDENTIFICATION NOTED BY PATHOLOGY ASSISTANT SHOULD CONTACT WITH
PATHOLOGISTS AND / OR CLINICIAN OF THERE ARE ANY QUESTIONS.
CAUSES OF REJECTION OF SPECIMEN
• SPECIMEN NOT IN FORMALIN.
• UNLABELED OR IMPROPERLY LABELED CONTAINER.
• WITHOUT REQUISITION SLIP OR INCOMPLETE REQUISITION SLIP.
16. ENDOSCOPIC BIOPSIES.
• ALL FRAGMENTS SHOULD BE SUBMITTED IN
THE SAME CONTAINER.
• NUMBER OF FRAGMENTS, AGGREGATE
DIMENSION.
• COLOUR AND CONSISTENCY SHOULD NOT BE
CUT OR INKED.
• ALL SMALL BIOPSIES MUST BE SUPPORTED
WITHIN THE CASSETTES TO PREVENT TISSUE
LOSS DURING PROCESSING.
• SMALL FRAGMENTS MAY BE DIPPED IN EOSIN
TO MAKE THEM VISIBLE.
• DO NOT SUBMIT ENDOSCOPIC BIOPSIES
WRAPPED IN GAUZE.
20. • TO BE ABLE TO STUDY MICROSCOPIC FEATURES OF TISSUE IT SHOULD
UNDERGO CERTAIN STEPS.
• WE ARE GOING TO TALK ABOUT THESE STEPS BRIEFLY.
21.
22.
23. TISSUE FIXATION.
• SHOULD PREVENT AUTOLYSIS AND PUTREFACTION OF THE CELL.
• SHOULD PENETRATE EVENLY AND RAPIDLY.
• SHOULD HARDEN THE TISSUES.
• INCREASE THE OPTICAL DENSITY.
• SHOULD NOT CAUSE SHRINKAGE OR SWELLING OF THE CELLS.
• MUST NOT REACT WITH THE RECEPTOR SITES AND THIS MUST NOT INTERFERE
WITH THE STAINING PROCEDURE.
• MUST BE CHEAP AND EASILY AVAILABLE.
24.
25.
26. TISSUE FIXATION.
• SPECIMEN SUBMIT IN 10% FORMALIN.
• FORMALIN TISSUE RATIO 10:1
• NO OTHER FIXATIVE SHOULD BE USED.
• SPECIMEN SHOULD BE IN A CONTAINER THAT CAN BE SEALED AND WILL NOT LEAK.
FRESH TISSUE TO BE SUBMITTED IN THE CASES OF:-
• FROZEN SECTION
• CULTURES
• IMMUNOFLOURESCENCE
• FLOWCYTOMETRY
• CHROMOSOME STUDIES
• ELECTRON MICROSCOPY
27. What is tissue processing?
• Tissue processing is a procedure of removing water from cells and
replacing it with a medium which solidifies allowing thin sections to
be cut on a microtome.
• Tissue processing is routinely done on an instrument called Tissue
Processor.
28.
29.
30.
31.
32.
33.
34.
35.
36. TISSUE PROCESSING
• THE TECHNIQUE OF GETTING FIXED TISSUE INTO PARAFFIN IS CALLED TISSUE PROCESSING, THE MAIN STEPS
IN THIS PROCESS ARE DEHYDRATION AND CLEARING.
• WET FIXED TISSUES CANNOT BE DIRECTLY INFILTRATED WITH PARAFFIN, FIRST THE WATER FROM THE
TISSUES MUST BE REMOVED BY DEHYDRATION.
• THE NEXT STEP IS CALLED “CLEARING” AND CONSISTS OF REMOVAL OF THE DEHYDRANT WITH A SUBSTANCE
THAT WILL BE MISCIBLE WITH THE EMBEDDING MEDIUM (PARAFFIN).
• FINALLY THE TISSUE IS INFILTRATED WITH THE EMBEDDING AGENT, ALMOST ALWAYS PARAFFIN.
37. • TISSUES THAT COME OFF THE TISSUE PROCESSOR ARE STILL IN THE CASSETTES AND MUST BE MANUALLY
PUT INTO THE BLOCKS BY A TECHNICIAN WHO MUST PICK THE TISSUES OUT OF THE CASSETE AND POUR
MOLTEN PARAFFIN OVER THEM.
• THIS “EMBEDDING” PROCESS IS VERY IMPORTANT, BECAUSE THE TISSUES MUST BE ALIGNED, OR ORIENTED,
PROPERLY IN THE BLOCK OF PARAFFIN.
38.
39. SECTIONING
• ONCE THE TISSUES HAVE BEEN EMBEDDED, THEY MUST BE
CUT INTO SECTIONS THAT CAN BE PLACED ON A SLIDE.
• THIS IS DONE WITH A MICROTOME, THE IMPORTANT THING
FOR PROPER SECTIONING IS A VERY SHARP KNIFE.
• MICROTOMES HAVE A MECHANISM FOR ADVANCING THE
BLOCK ACROSS THE KNIFE, USUALLY THIS DISTANCE CAN BE
SET, FOR MOST PARAFFIN EMBEDDED TISSUES AT 6 TO 8
MICRONS.
• SECTIONING TISSUES IS A REAL ART AND TAKES MUCH SKILL
AND PRACTICE, IT IS IMPORTANT TO HAVE A PROPERLY FIXED
AND EMBEDDED BLOCK OR MUCH ARTEFACT CAN BE
INTRODUCED IN THE SECTIONING.
• COMMON ARTEFACTS INCLUDE TEARING, HOLES, FOLDING
ETC.
• ONCE SECTIONS ARE CUT THEY ARE FLOATED ON A WARM
WATER BATH THAT HELPS REMOVE WRINKLES.
• THEN THEY ARE PICKED UP ON A GLASS MICROSCOPIC SLIDE.
• THE GLASS SLIDES ARE THEN PLACED IN A WARM OVEN FOR
ABOUT 15 MINUTES TO HELP THE SECTION ADHERE TO THE
SLIDE.
40.
41. Staining
If the slide is examined under microscope without
staining we can see only vague shadows of the tissue
To visualize the tissue and its cellular components we
have to use dyes which stain the different components
according to their chemical compositions
42. STAINING AND COVER SLIPPING
• THE EMBEDDING PROCESS MUST BE REVERSED IN ORDER TO GET THE PARAFFIN WAX OUT OF THE TISSUE
AND ALLOW WATER SOLUBLE DYES TO PENETRATE THE SECTIONS.
• THEREFORE, BEFORE ANY STAINING CAN BE DONE, THE SLIDES ARE “DEPARAFFINIZED” BY RUNNING THEM
THROUGH XYLENES TO ALCOHOLS TO WATER.
• THERE ARE NO STAINS THAT CAN BE DONE ON TISSUES CONTAINING PARAFFIN.
• THE STAINED SECTION ON THE SLIDE MUST BE COVERED WITH A THIN PIECE GLASS TO PROTECT THE TISSUE
FROM BEING SCRATCHED, TO PROVIDE BETTER OPTICAL FOR VIEWING UNDER THE MICROSCOPE AND TO
PRESERVE THE TISSUE SECTION FOR YEARS TO COME.
• THE STAINED SLIDE MUST GO THROUGH THE REVERSE PROCESS THAT IN WENT THROUGH FROM PARAFFIN
SECTION TO WATER.
43.
44.
45.
46.
47.
48.
49. FROZEN SECTION
• FROZEN SECTIONS ARE PERFORMED WITH AN
INSTRUMENT CALLED A CRYOSTAT.
• THE CRYOSTATE IS JUST A REFRIGERATED BOX
CONTAINING A MICROTOME.
• THE TEMPRATURE INSIDE THE CRYOSTATE IS
ABOUT -20 TO -30 DEGREE C.
• THE TISSUE SECTIONS ARE CUT AND PICKED UP ON
A GLASS SLIDE.
• THE SECTIONS ARE DRIED AND THEN STAINED.
50. MICROWAVE PROCESSING
• WITHIN THE LAST DECADE, TISSUE PROCESSING WITH THE MICROWAVE OVEN HAS BEEN INTRODUCED INTO
HISTOLOGY LABORATORIES.
• PROCESSING TIMES ARE SIGNIFICANTLY REDUCED BUT THROUGHPUT IS VERY LOW.
• ONLY LABORATORY MICROWAVE OVERNS SHOULD BE USED AS THE TEMPRATURE MUST BE CAREFULLY
CONTROLLED AND THE MICROWAVE MUST BE VENTED JUST LIKE A CHEMICAL FUME HOOD.
• THIS TECHNOLOGY IS ESPECIALLY USEFUL FOR BIOPSY SIZED SPECIMENS.
• REAGENTS USED IN MICROWAVE PROCESSING INCLUDE:- ETHYL ALCOHOL, ISOPROPYL ALCOHOL AND
PARAFFIN.
51. • Proper orientation of the tissue samples is important for a
correct diagnosis of malabsorptive states such as celiac
disease, where the ratio villous height – crypt depth must be
assessed and for specimens from endoscopic resections of
polyps or early neoplastic lesions.
• Multiple sections from multiple endoscopic biopsies allow a
more complete microscopic analysis
52. Immunohistochemistry and other ancillary
techniques
• Histopathology is an adequate tool for solving differential diagnostic
problems and typing of tumours.
• Anaplastic carcinomas, large-cell lymphoma, epithelioid stromal tumours
and neuroendocrine tumours can be difficult but immunohistochemical
stainings with antibodies against cytokeratins (CK), a marker for epithelial
cells,
• CD117 a marker for gastrointestinal stromal tumors,
• chromogranin, a marker for endocrine cells and a
• common leucocyte marker can solve the problem.
• Antibodies to intermediate filaments such as the CKs can be potentially
useful in other situations. CKs comprise a subfamily of more than 20
members.
• CK7 and CK20 & examination of coordinate expression of these two CKs
can help in the differential diagnosis of carcinomas of unknown primary
site.
53. • Immune histochemistry and cytogenetic analysis is essential for the
management of lymphomas. Primary intestinal lymphomas should
be sub-typed in B cell and T cell malignancies and classified
according internationally validated classifications such as the
recently published WHO.
• Evaluating the proliferation fraction of the tumour cells using a
marker such as Ki67 or MIB1 may provide some additional
information on the biological behaviour of lymphomas. This is also
true for endocrine tumours and gastrointestinal stromal tumours.
Further ancillary techniques may include staining with antibodies
against p53 for Barret’s oesophagus or colitis-associated dysplasia.
Currently a number of markers are under investigation for a more
accurate identification of early neoplasia.
54. • Histochemistry (histological special stains) searching for mucins or
other substances, and occasionally electron microscopy and genetic
markers can also be applied on biopsy samples. Many stainings can
be performed on routinely formalin fixed material. Increasingly there
is some overlap, between immune histochemistry and molecular
techniques since genetic markers can be demonstrated also by
immune histochemistry. This is for instance true for large-bowel
cancers with microsatellite instability (MS), where the products of the
DNA repair genes hMLH1, hMSH2 and MSH6, or the lack of them,
can be demonstrated immune histochemically. These products do
not however cover the whole range of MS. DNA or RNA extraction
and genetic analysis remains important and there may even be a
growing need.