This document summarizes cell-based assay solutions from Neuromics and its partnership with UB Systems. It describes 3D cell-based assays including blood-brain barrier models and extracellular matrix hydrogels. It also provides details on various human brain cell types such as endothelial cells, astrocytes, and pericytes that can be used to build customized 3D brain models. Additionally, it mentions services offered like media filling and various cell culture reagents including fetal bovine serum in different grades.

1. CHARACTERIZED, TESTED AND DISCOVERY
READY
Solutions Designed for Drug Discovery

2. NEUROMICS AND UB SYSTEM’S BUSINESS
PARTNERSHIP
A) 2 and 3-D Cell Based Assays
B) Manufacture and High-Speed
Filling of Media
Sales and Support Manufacturing
Neuromics UB Systems

3. 3-D CELL-BASED ASSAY SOLUTIONS
 3D Models:
 3-D Blood Brain Barrier
Models (left image – ACE-2
staining (red) and DAPI
nuclear counterstain (blue) of
the endothelial cells on the
bottom of the inserts).
 Extracellular Matrixes
• Engineered hydrogels
optimized to cell types
• Nanofibers
 Coating Solutions
 Alphabiocoat (right image)
increases cell attachment and
growth

4. HUMAN BBB MODEL-PUB AND DATA
 New Reference-Sookhee Bang, Jeong
Kuen Song, Seung-Wook Shin, and Kwan
Hee Lee. (2021). Human Serum Albumin
Fusion Protein as Therapeutics for
Targeting Amyloid Beta in Alzheimer’s
Diseases. Neuroscience Letters. doi;
10.1016/j.neulet.2021.136298
 BBB Penetration Data (pdf)

5. HUMAN BRAIN ASTROCYTES
 Cat No. HMP202
 Source from non-fetal donors
 Average doubling capability of >10
 Recent publications:
 Sorel Kurbegovic, Karina Juhl, Kasper Kildegaard Sorensen, Julie Leth, Gro Linno
Willemoe,Anders Christensen,Yvonne Adams,Anja Ramstedt Jensen, ChristianVon
Buchwald, Jane Skjoth-Rasmussen, Michael Ploug, Knud J. Jensen,Andreas Kjaer. (2021).
IRDye800CW Labeled uPAR-Targeting Peptide for Fluorescence-Guided Glioblastoma
Surgery: Preclinical Studies in Orthotopic Xenografts.Theranostics, 11(15): 7159-7174.
doi: 10.7150/thno.49787
 Yvonne Adams, Rebecca W. Olsen,Anja Bengtsson, Nanna Dalgaard, Mykola Zdioruk,
Sanghamitra Satpathi, Prativa K. Behera, Praveen K. Sahu, Sean E. Lawler, Klaus Qvortrup,
Samuel C.Wassmer, and Anja T.R. Jensen. (2021). Plasmodium Falciparum Erythrocyte
Membrane Protein 1Variants Induce Cell Swelling and Disrupt the Blood–Brain Barrier
in Cerebral Malaria. Journal of Experimental Medicine, 218 (3). doi:
10.1084/jem.20201266
 Top image:Astrocytes stained with GFAP (cat no. SP15101)
 Bottom Image:Astrocytes stained with S100 beta (cat no. GT15160)

6. HUMAN BRAIN ENDOTHELIAL CELLS
 Cat No. HEC02
 Source from non-fetal donors
 Average doubling capability of >15
 Recent publications:
 Pasquale Mone, Jessica Gambardella, Xujun Wang, Stanislovas S. Jankauskas,Alessandro Matarese,
and Gaetano Santulli. (2021). miR-24 Targets the Transmembrane Glycoprotein Neuropilin-1 in
Human Brain Microvascular Endothelial Cells. Non-coding RNA, 7, 9. doi:
10.3390/ncrna7010009
 YangWu,Yan Li, Liangqing Zheng, PengchengWang,Yan liu,YongningWu, ZhiYong Gonga (2019).
The neurotoxicity of Nε-(carboxymethyl)lysine in food processing by a study based on animal
and organotypic cell culture. Ecotoxicology and Environmental Safety.
https://doi.org/10.1016/j.ecoenv.2019.110077
 Top image: HBMECS stained with CD146
 Bottom Image: HBMECs stained with Claudin-5

7. RETINAL CELLS
 Retinal Endothelial Cells - cat no. HEC09
 Recently publications:
 A. Gericke, K. Suminska-Jasinska, and A. Breborowicz. (2021). Sulodexide Reduces
Glucose Induced Senescence in Human Retinal Endothelial Cells. Scientific Reports,
11:11532. doi: 10.1038/s41598-021-90987-w
 Fei Cai, Huanzong Jiang,Yan Li, Qin Li, and ChaoYang. (2021). Up-Regulation of Long
Non-Coding RNA SNHG16 Promotes Diabetes-Related Retinal Microvascular
Endothelial Cell Dysfunction via Activating NF-κB and PI3K/AKT Pathways.
Molecular Therapy: Nucleic Acid. doi: 10.1016/j.omtn.2021.01.035
 Images: Cells were stained for CD31 (green) and vWF (red). Overlap of
expression of CD31 and vWF is can be seen in the yellow color (expression
of both green and red)
 Retinal Pigment Epithelial Cells – cat no. RPE001

8. CELLS IN CUSTOMERS HANDS – BBB MODEL CREATION
Highlights Researchers using our Human Pericytes, Brain
Microvascular Endothelial Cells (HBMES) and Astrocytes to build a
static 3-D Blood-Brain Barrier Model. Ece Bayir, M. Mert Celtikoglu,
Aylin Sendemira.The use of bacterial cellulose as a basement
membrane improves the plausibility of the static in vitro blood-brain
barrier model. https://doi.org/10.1016/j.ijbiomac.2018.12.257.
Image: Pericytes, HBMECS, and Astrocytes 5 days after culturing. Live cells (green) and dead
cells (red).
The investigators concluded, "Caffeine and sucrose permeability
values obtained from all models were close to literature data and
physiological values."

9. FBS AND OTHER SERA
We currently offer 3 different lines of regular FBS - Premium, Standard, and
Qualified.All 3 lines are available in both U.S. origin and Imported origin (USDA
approved).The lines of FBS are differentiated by the endotoxin levels in each lot.
• Our Premium FBS endotoxin specification is ≤10 EU/mL.
• Our Standard FBS endotoxin specification is ≤30 EU/mL.
• Our Qualified FBS endotoxin specification is ‘As reported’
Chicken, Donkey, Horse and Rabbit Serum available upon request

10. ENGINEERED HYDRO COLLAGELS
Neuromics’ 3-D HUVEC
Model
Our CollaGel Hydrogel matches the softness of the body's
tissues. It can be used to culture neurons in an
environment as soft as the brain, cardiomyocytes on the
stiffness of muscle, or whatever your cells' tissue of origin
may be. In a 3D tissue model, our CollaGel Hydrogel helps
the cells build stronger basement membranes. Collagel
Hyrogels can be used for: Stem Cell Behavior Studies
Fibrosis Studies, Cosmetic Toxicology, Hepatocyte Assays,
Neuronal Branching, Wound Healing Assays, Cell Invasion
Assays, Migration Assays, Cancer Cell Phenotyping and
Bioprinting. https://www.neuromics.com/3-d-cell-based-
assay-solutions

11. Phone: 952-374-616
Fax: 612-677-3976
Email: david@neuromics.com for orders
pshuster@neuromics.com
We welcome your input, feedback and questions:
Pete Shuster-CEO and Owner
Direct Phone: 612-801-1007
Email: pshuster@neuromics.com
5235 W 74th Street Suite 8
Edina, MN, 55439
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