This document summarizes experiments using Gibson assembly to insert the Neon gene into a vector. It outlines the steps taken, including modifying PCR primers, testing reactions with new primers, comparing HF and Taq polymerases, optimizing temperature and PCR conditions, and estimating the percentage of the Neon gene present in samples. Future steps discussed are colony PCR to verify the Neon insert, preparing colonies for sequencing, and analyzing the DNA sequences.