1. Modification of cardiomyocytes

2. * Heart Disease is one of the major killers in Irish
society today; this is the basics of our project.
We shall bring the technology of photo
sensitive cells, cloning of heart cells cultured
to combine with their photo reactive potential,
*

3. Light sensitive Protein ------- Embedded into DNA of Cardio Cells
CHANNELRHODSIN2 (CHR2) ----Stem cells of CARDOMYOCYTES +Light(Energy)
COMBINED with
Pravastatin
and Heparin
Using latest LED Technology of light frequencies to transfer Data
wirelessly :Result
Pacemaker for the Heart
Powered By
LIGHT

4. Antimicrobial materials,
Ultraviolet Germicidal Irradiation considered into design
Light source
Translucent, Implant
External cartridge Slots
Internal Implant
Into Implant
External, Cartridge can be removed
Pulsation of High intensive light Cartridge cell capacity 200ml
waves, from device allows Light
wavelengths to pass through the Treatment options
translucent implant to react with
20 Days = 200mls
the heart cells, allowing for
contraction to occur. In the ratio of 1 Day = 20mls
one pulse of light: one beat of heart
1hr = 1.2mls
Drug Dosage option per 200ml
Solution Ratio = 2:1
Pravastatin - 0.4µg (mcg) per 200 ml
Heparin - 1.6 (mcg) per 200 ml Hourly Rate= cell culture 0.8ml: 0.4ml Drug

5. Echocardiogram could be a useful
tool, In motoring the activities of the
heart in patient’s also , with the
technology of wireless data transfer by
light, (pluses of light) this could in
theory have a safety feature built into
the design of the prototype,

7. With the careful design of a 3D model, using a cartridge based
implant allowing pulsation of high intensity light and introduce the
cultured cells to the heart. We must saturate the infected heart with
these cells, So in using current technology the frequency of the
wavelength of light can stream data in so much compared with Wi-Fi
radio waves, this latest technology would be useful to our project as
it could give statistical representations for the concentration of the
photo cultured cells and in theory notify the local hospital or GP of
risk to the patient, monitor heart rhythms, depletion of cells etc.

8. *Cardiomyocytes
* Scientists have found cardiomyoctes can be grown from
induced pluripotent stem cells (iPS) by reprogramming a
persons blood cells (these (iPS) cells can mimic and can turn
into any of the cell types in the human body).
Stemming from this discovery researchers have found it
possible to convert fibroblasts into cells that can beat like
cardiomyoctes by introducing factors of encoded proteins
that have a specific activity or interaction on the DNA that
in turn mimic their effect (i.e cardiomyctes) when
introduced directly into the heart. Half the cells in the
heart are fibroblasts, and so calling on these reservoir of
cells has the ability to result in cardiac regeneration and
other therapeutic potential; a proposition that is
particularly enticing because these cells wouldn't be
rejected by the host's body..

9. *Cell Culture and Harvesting
• By taking samples of Heart Cells from the donor and by identifying and
converting those healthy fibroblasts into cardiomyoctes like cells we can
then go through the process of cell culture and harvesting by current
approved methods and protocols taking into account all intrinsic
environmental parameters to include in summary:
* Work area and equipment - flow hoods, Incubators, Microscopes,
Preservation, Vessels
* Cell Preservation and storage
* Cell Maintenance - growth pattern, harvesting, media and growth
requirements
* Safety considerations
* Tissue culture methods
* Determining cell counts

10. *
Cardiomyocytes reproduction rate can be significantly increased
by using certain drugs or drug combinations in addition to the
cultured cells in the treatment liquid.
Our drug selection depends on the chosen application and
damage type:
• Pravastatin – reduces cholesterol and stimulates cell
reproduction .
* Concentration: 0.4 µg (mcg) per 200 ml of the treatment
liquid
• Heparin (vessel injection only) – prevents blood clotting and
improves blood circulation (which will help cell delivery to
the damaged area)
* Concentration: 1.6 µg (mcg) per 200 ml of the treatment
liquid

11. Pravastatin
Pravastatin is a medication belonging to the
drug class known as HMG-CoA reductase
inhibitors, also called "statins." It is used to
lower blood cholesterol and reduce the risk of
heart attack, stroke and death due to
arteriosclerotic vascular disease
• The introduction of pravastatin and cell culture into the
damaged area of the myocardium or into the coronary
blood system stimulates the regeneration of
cardiomyocytes directly in the damaged area.
• Also, pravastatin has a function of lowering cholesterol
levels in blood and thus can lead to an overall better
blood circulation on the coronary system.

12. Heparin
Heparin is a heterogeneous group of
straight-chain anionic
mucopolysaccharides, called
glycosaminoglycans, having anticoagulant
properties. Heparin is administered for
treatment of venous thrombosis or acute
myocardial ischemia
• When treatment liquid is introduced into the
coronary blood system, the risk of blood clotting
rises.
• In this case, we use heparin to prevent thrombosis.
This, in turn, leads, to a better delivery of the
treatment liquid to the damaged area.

13. *
By inserting the light sensitive protein (CHR2) into the stem
cell of the new harvested cardiomyoctes.
It is the light sensitive protein that will control the flow of
ions, especially sodium, in and out of the cells and
therefore manage their pacing; i.e. when the protein is
exposed to a special wavelength of blue light, it opens
channels in the cell membrane to allow an influx of sodium,
creating a contraction.
So together with the selected drugs dependant on the
specific condition and introduced directly into the coronary
blood system, the healing process can begin.
We strongly believe that the use of the combination of
light, cell culture and drugs in the treatment of the
damaged myocardium will lead to the regeneration of
cardiomyocytes and the rapid recovery of the injured areas.

14. *