Mise-en-scene refers to everything visible within the frame of a shot that contributes to the audience's understanding, including lighting, costumes, props, sets/locations, blocking/performance, and color. The document discusses these six key elements of mise-en-scene and provides examples to illustrate how each element is used to convey meaning and create mood. Props, costumes, blocking/performance, lighting, and location/set are analyzed in short examples and questions to demonstrate their role in visual storytelling.