The document summarizes bacterial growth kinetics and growth curves. It discusses:
1) Bacterial growth occurs through binary fission, where a single cell divides into two identical cells. This allows bacterial populations to increase exponentially over time.
2) A bacterial growth curve consists of four phases - lag phase, logarithmic/exponential phase, stationary phase, and decline phase - with transitions between each. The logarithmic phase sees the fastest growth rate.
3) Generation time is the time it takes for a bacterial population to double and varies between species and growth conditions. The reciprocal of generation time is the growth rate constant. Common generation times range from 15 minutes to 24 hours.
When fresh liquid medium is inoculated with a given number of bacteria and incubated for sufficient period of time, it gives a characteristic growth pattern of bacteria.
If the bacterial population is measured periodically and log of number of viable bacteria is plotted in a graph against time, it gives a characteristic growth curve which is known as growth curve or growth cycle.
When fresh liquid medium is inoculated with a given number of bacteria and incubated for sufficient period of time, it gives a characteristic growth pattern of bacteria.
If the bacterial population is measured periodically and log of number of viable bacteria is plotted in a graph against time, it gives a characteristic growth curve which is known as growth curve or growth cycle.
MICROBIAL GROWTH, REPRODUCTION AND CONTROLPeterKenneth3
Microbial growth is defined as an increase in the number of cells. A microbial cell has a lifespan and a species is maintained only as a result of continued growth of its population. Growth is the ultimate process in the life of a cell – one cell becoming two and subsequently leading to an increase in the number in a population of microorganisms.
In microbiology, growth is synonymous to reproduction. This unit examines the term growth, binary fission, the mode of cell division in prokaryotic cells, stages in the growth curve and the mathematics of growth.
Definition of Growth
Growth is defined as an increase in the number of cells in a population of microorganisms. It is an increase in cellular constituents leading to arise in cell number when microorganisms reproduce by processes like binary fission or budding.
The Prokaryotic Cell Cycle
A prokaryotic cell cycle is the complete sequence of events from the formation of a new cell through the next division. Most prokaryotes reproduce by binary fission, budding or fragmentation.
Binary Fission
Binary fission is a form of asexual reproduction process. In which a single cell divides into two cells after developing a transverse septum(cross wall).Binary fission is a simple type of cell division and the processes involved are: the cell elongates, replicates its
chromosomes and separates the newly formed DNA molecules so that there is a chromosome in each half of the cell. A septum is formed at mid cell; divide the parent cell into two progeny cells and each having its own chromosome and a copy or complement of other cellular constituents.
Defination,growth curve, types and kinetics of growth curve, applications and advantages and disadvantages . Environmental factors affecting the cell growth.
Bacteria are microscopic, single-celled organisms that thrive in diverse environments. These organisms can live in soil, the ocean and inside the human gut. Humans' relationship with bacteria is complex. Sometimes bacteria lend us a helping hand, such as by curdling milk into yogurt or helping with our digestion
Microbial Growth and Nutrition, and Clones, Enzymes and Informative Hybridiza...Akram Hossain
This was an assignment of preparing “A lecture note on Microbial Growth and Nutrition, and Clones, Enzymes and Informative Hybridizations” for the course "General Microbiology"
Hope you will find it useful.
MICROBIAL GROWTH, REPRODUCTION AND CONTROLPeterKenneth3
Microbial growth is defined as an increase in the number of cells. A microbial cell has a lifespan and a species is maintained only as a result of continued growth of its population. Growth is the ultimate process in the life of a cell – one cell becoming two and subsequently leading to an increase in the number in a population of microorganisms.
In microbiology, growth is synonymous to reproduction. This unit examines the term growth, binary fission, the mode of cell division in prokaryotic cells, stages in the growth curve and the mathematics of growth.
Definition of Growth
Growth is defined as an increase in the number of cells in a population of microorganisms. It is an increase in cellular constituents leading to arise in cell number when microorganisms reproduce by processes like binary fission or budding.
The Prokaryotic Cell Cycle
A prokaryotic cell cycle is the complete sequence of events from the formation of a new cell through the next division. Most prokaryotes reproduce by binary fission, budding or fragmentation.
Binary Fission
Binary fission is a form of asexual reproduction process. In which a single cell divides into two cells after developing a transverse septum(cross wall).Binary fission is a simple type of cell division and the processes involved are: the cell elongates, replicates its
chromosomes and separates the newly formed DNA molecules so that there is a chromosome in each half of the cell. A septum is formed at mid cell; divide the parent cell into two progeny cells and each having its own chromosome and a copy or complement of other cellular constituents.
Defination,growth curve, types and kinetics of growth curve, applications and advantages and disadvantages . Environmental factors affecting the cell growth.
Bacteria are microscopic, single-celled organisms that thrive in diverse environments. These organisms can live in soil, the ocean and inside the human gut. Humans' relationship with bacteria is complex. Sometimes bacteria lend us a helping hand, such as by curdling milk into yogurt or helping with our digestion
Microbial Growth and Nutrition, and Clones, Enzymes and Informative Hybridiza...Akram Hossain
This was an assignment of preparing “A lecture note on Microbial Growth and Nutrition, and Clones, Enzymes and Informative Hybridizations” for the course "General Microbiology"
Hope you will find it useful.
Immunizing Image Classifiers Against Localized Adversary Attacksgerogepatton
This paper addresses the vulnerability of deep learning models, particularly convolutional neural networks
(CNN)s, to adversarial attacks and presents a proactive training technique designed to counter them. We
introduce a novel volumization algorithm, which transforms 2D images into 3D volumetric representations.
When combined with 3D convolution and deep curriculum learning optimization (CLO), itsignificantly improves
the immunity of models against localized universal attacks by up to 40%. We evaluate our proposed approach
using contemporary CNN architectures and the modified Canadian Institute for Advanced Research (CIFAR-10
and CIFAR-100) and ImageNet Large Scale Visual Recognition Challenge (ILSVRC12) datasets, showcasing
accuracy improvements over previous techniques. The results indicate that the combination of the volumetric
input and curriculum learning holds significant promise for mitigating adversarial attacks without necessitating
adversary training.
Welcome to WIPAC Monthly the magazine brought to you by the LinkedIn Group Water Industry Process Automation & Control.
In this month's edition, along with this month's industry news to celebrate the 13 years since the group was created we have articles including
A case study of the used of Advanced Process Control at the Wastewater Treatment works at Lleida in Spain
A look back on an article on smart wastewater networks in order to see how the industry has measured up in the interim around the adoption of Digital Transformation in the Water Industry.
Hierarchical Digital Twin of a Naval Power SystemKerry Sado
A hierarchical digital twin of a Naval DC power system has been developed and experimentally verified. Similar to other state-of-the-art digital twins, this technology creates a digital replica of the physical system executed in real-time or faster, which can modify hardware controls. However, its advantage stems from distributing computational efforts by utilizing a hierarchical structure composed of lower-level digital twin blocks and a higher-level system digital twin. Each digital twin block is associated with a physical subsystem of the hardware and communicates with a singular system digital twin, which creates a system-level response. By extracting information from each level of the hierarchy, power system controls of the hardware were reconfigured autonomously. This hierarchical digital twin development offers several advantages over other digital twins, particularly in the field of naval power systems. The hierarchical structure allows for greater computational efficiency and scalability while the ability to autonomously reconfigure hardware controls offers increased flexibility and responsiveness. The hierarchical decomposition and models utilized were well aligned with the physical twin, as indicated by the maximum deviations between the developed digital twin hierarchy and the hardware.
Using recycled concrete aggregates (RCA) for pavements is crucial to achieving sustainability. Implementing RCA for new pavement can minimize carbon footprint, conserve natural resources, reduce harmful emissions, and lower life cycle costs. Compared to natural aggregate (NA), RCA pavement has fewer comprehensive studies and sustainability assessments.
Industrial Training at Shahjalal Fertilizer Company Limited (SFCL)MdTanvirMahtab2
This presentation is about the working procedure of Shahjalal Fertilizer Company Limited (SFCL). A Govt. owned Company of Bangladesh Chemical Industries Corporation under Ministry of Industries.
About
Indigenized remote control interface card suitable for MAFI system CCR equipment. Compatible for IDM8000 CCR. Backplane mounted serial and TCP/Ethernet communication module for CCR remote access. IDM 8000 CCR remote control on serial and TCP protocol.
• Remote control: Parallel or serial interface.
• Compatible with MAFI CCR system.
• Compatible with IDM8000 CCR.
• Compatible with Backplane mount serial communication.
• Compatible with commercial and Defence aviation CCR system.
• Remote control system for accessing CCR and allied system over serial or TCP.
• Indigenized local Support/presence in India.
• Easy in configuration using DIP switches.
Technical Specifications
Indigenized remote control interface card suitable for MAFI system CCR equipment. Compatible for IDM8000 CCR. Backplane mounted serial and TCP/Ethernet communication module for CCR remote access. IDM 8000 CCR remote control on serial and TCP protocol.
Key Features
Indigenized remote control interface card suitable for MAFI system CCR equipment. Compatible for IDM8000 CCR. Backplane mounted serial and TCP/Ethernet communication module for CCR remote access. IDM 8000 CCR remote control on serial and TCP protocol.
• Remote control: Parallel or serial interface
• Compatible with MAFI CCR system
• Copatiable with IDM8000 CCR
• Compatible with Backplane mount serial communication.
• Compatible with commercial and Defence aviation CCR system.
• Remote control system for accessing CCR and allied system over serial or TCP.
• Indigenized local Support/presence in India.
Application
• Remote control: Parallel or serial interface.
• Compatible with MAFI CCR system.
• Compatible with IDM8000 CCR.
• Compatible with Backplane mount serial communication.
• Compatible with commercial and Defence aviation CCR system.
• Remote control system for accessing CCR and allied system over serial or TCP.
• Indigenized local Support/presence in India.
• Easy in configuration using DIP switches.
Saudi Arabia stands as a titan in the global energy landscape, renowned for its abundant oil and gas resources. It's the largest exporter of petroleum and holds some of the world's most significant reserves. Let's delve into the top 10 oil and gas projects shaping Saudi Arabia's energy future in 2024.
Final project report on grocery store management system..pdfKamal Acharya
In today’s fast-changing business environment, it’s extremely important to be able to respond to client needs in the most effective and timely manner. If your customers wish to see your business online and have instant access to your products or services.
Online Grocery Store is an e-commerce website, which retails various grocery products. This project allows viewing various products available enables registered users to purchase desired products instantly using Paytm, UPI payment processor (Instant Pay) and also can place order by using Cash on Delivery (Pay Later) option. This project provides an easy access to Administrators and Managers to view orders placed using Pay Later and Instant Pay options.
In order to develop an e-commerce website, a number of Technologies must be studied and understood. These include multi-tiered architecture, server and client-side scripting techniques, implementation technologies, programming language (such as PHP, HTML, CSS, JavaScript) and MySQL relational databases. This is a project with the objective to develop a basic website where a consumer is provided with a shopping cart website and also to know about the technologies used to develop such a website.
This document will discuss each of the underlying technologies to create and implement an e- commerce website.
Sachpazis:Terzaghi Bearing Capacity Estimation in simple terms with Calculati...Dr.Costas Sachpazis
Terzaghi's soil bearing capacity theory, developed by Karl Terzaghi, is a fundamental principle in geotechnical engineering used to determine the bearing capacity of shallow foundations. This theory provides a method to calculate the ultimate bearing capacity of soil, which is the maximum load per unit area that the soil can support without undergoing shear failure. The Calculation HTML Code included.
2. TOPIC NAME
KINETICS OF BIOLOGICAL GROWTH, BACTERIAL
GROWTH IN TERMS OF NUMBERSAND MASS, GROWTH
CURVE
PRESENTED BY
ONKAR NANDKUMAR SANGAR
UNDER GUIDANCE OF
DR. P. B. BHAVE
2
3. INTRODUCTION
• Cell growth implies increase in its mass and physical size controlled
by physical, biological and chemical environments.
• Microbial growth is quantified by increase inhe macromolecular and
chemical constituents of the cell and growth pattern of each microbe is
unique.
• Growth kinetics is an autocatalytic reaction which implies that the rate
of growth is directly proportional to the concentration of cell.
• Microbial growth kinetics explains the relationship between the
specific growth rate of a microbe and its substrate concentration. 3
4. BIOLOGICAL GROWTH
• Biological exponential growth is the unrestricted growth of a population of
organisms, occurring when resources in its habitat are unlimited.
• Most commonly apparent in species that reproduce quickly and asexually,
like bacteria, exponential growth is intuitive from the fact that each organism
can divide and produce two copies of itself.
• Each descendent bacterium can itself divide, again doubling the population
size. The bacterium Escherichia coli, under optimal conditions, may divide
as often as twice per hour.
• Left unrestricted, a colony would cover the Earth's surface in less than a
day.
4
5. KINETICS OF BIOLOGICAL GROWTH IN
ANIMAL
• The growth of animals is more restricted
in time than is that of plants.
• but cell division is more generally
distributed throughout the body of the
organism.
• Although the rate of cell division differs in
different regions, the capacity for cell
division is widely distributed in the
developing embryo.
• Cell division and size increase continue,
however, even after increase in total body
size no longer occurs. Because these events
are balanced by cell death
5
6. • Lag phase
The lag phase which occurs immediately after inoculation
and persists until the cells have acclimated to their new
environment.
• Exponential Growth phase
Exponential phase, during which time cell growth
proceeds at an exponential rate (indicated by a straight
line on the semi-log plot).
• Deceleration phase
when essential nutrients are depleted or toxic products
begin to accumulate. 6
7. • Stationary phase
during which time the net cell growth is approximately
zero.
• Death phase
where some cells loose viability or are destroyed by lysis
7
8. KINETIC OF BIOLOGICAL GROWTH IN
PLANT
• The total period from initial to the final stage of
growth is called the grand period of
growth. The total growth is plotted against time
and ‘S’ shaped sigmoid curve (Grand period
curve) is obtained.
i. Lag phase
ii. Log phase
iii. Decelerating phase
iv. Maturation phase
8
9. • i. Lag phase
In this phase new cells are formed from pre-existing cells slowly. It is found in
the tip of the stem, root and branches. It is the initial stage of growth. In other words,
growth starts from this period.
• ii. Log phase or exponential growth
Here, the newly formed cell increases in size rapidly by deposition of cell wall
material. Growth rate is maximum and reaches top because of cell division and
physiological processes are quite fast. The volume of protoplasm also increases. It
results in rapid growth and causes elongation of internode in the stem.
• iii. Decelerating phase or Decline phase or slow growth phase
The rate of growth decreases and becomes limited owing to internal and external
or bot the factors because the metabolic process becomes slow.
9
10. • iv. maturation phase
In this phase cell wall thickening due
to new particle deposition on the inner
surface of the cell wall takes place.
The overall growth ceases and
becomes constant. The growth rate
becomes zero.
10
11. BACTERIAL GROWTH
• Bacteria divide by Binary fIssion.
• Most pathogens grow on artificial culture media.
• Some pathogens have never been grow in
culture.
• Others only grow intracelluarly in tissue culture.
• Generation time is determined by available
nutrients, pH, temp. 11
12. BACTERIAL GROWTH IN LIQUID
MEDIUM
• Microorganisms can be cultivated either in liquid or
solid growth media.
• Liquid media cultivation is done either in small
Universals (up to 20 ml), in Erlenmeyer flasks (up to
1l) and fermenters (from 1l onwards up to excess of
100000 l).
• Methods of liquid cultivation:
1. Batch cultivation
2. Continuous cultivation 12
13. BINARY FISSION
• The normal reproductive method of bacteria is transverse binary
fission in which a single cell divides into two identical cells after
developing a cross wall (transverse)septum.
• It is an asexual reproductive process.
• Thus, bacteria increase their numbers by geometric progression
or exponential growth.
• bacterial population every generation as :
1, 2, 4, 8, etc. Then 2^0, 2^1, 2^2........2^n
(where n = the number of generations). 13
14. • In the center of bacterium, a group of proteins called Fts proteins form
a ring at the cell division plane called as divisome .
• During DNA replication, each strand of the replicating bacterial DNA
attaches to divisome .
• The bacterial cell membrane coordinates the process.
• The two daughter DNA molecules remain attached at divisome, side-
by-side, while new membrane and cell wall is synthesized as a
transverse septum in between the two newly formed chromosomes
• When septum formation is complete the cell splits into two
progenyprogeny cells.
14
16. OTHER MECHANISM OF BACTERIAL
GROWTH
• Budding
• Some bacteria reproduce by budding, a process in which a small protuberance
(bud) develops at one end of the cell, enlarges and develops into a new cell
that later separates from the parent cell.e.g.Rhodopseudomonas acidophila
• Fragmentation
• Bacteria that produces extensive filamentous growth reproduce by
fragmentation of the filament into small cells.e.g. Nocardia
• Formation of sporangiospores and Condiospores
• Some species of Streptomyces produce many spores per organism by
developing cross wall at the hyphal tip and each spore give rise to new
organisms.
16
17. KINETIC OF BACTERIAL GROWH
GROWTH RATE AND GENERATION TIME
• The time required for a bacterial cell to divide, i.e. a population to
double, during log-phase, is known as the generation time. For a
population, it is often called “Mean Generation Time”.
• Under a given set of growth conditions (medium, temperature,
pH,etc.) each bacterial species has a genetically determined
generation time.
• Typically, generation times range from about 12 minutes to 24 hours.
• Most bacteria of medical interest have generation times of 15 min. to
an hour or so.
• The reciprocal of the Generation Time (1/G) is called the “Growth
Rate Constant” (k, [generations/unit time]).
17
18. • The generation time is given by the formula:
G = t/n
where,
G = generation time
t = time interval in hours or minutes
n = number of generations
• Generation time of some bacteria as follows.
1. Escherichia coli - 17min.
2. Bacillus megaterium – 25min
3. Staphylococcus aureus - 27-30 min
4. Mycobacterium tuberculosis - 792-932 min 18
20. BACTERIAL GROWH CURVE
• When one measures the growth of a bacterial culture in a closed
system such as a flask or a fermenter and plots the logarithm of cell
number over time then one obtains a characteristic curve, which is
called a bacterial growth curve.
• The growth curve consists of four distinct phases, with a transition
period in between each phase:
1. Lag phase
2. Log (logarithmic or exponential) phase
3. Stationary phase (this is where organisms ‘spend’ most of their time).
4. Decline (death) phase 20