This document summarizes research presented at the XXVII Brazilian Congress in Biomedical Engineering on developing new drugs from maleimides to combat bacteria in tambaqui fish. Key findings include: - Synthesis and characterization of methyl 4-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl) benzoate, which achieved 68% yield and was supported by NMR and mass spectrometry data. - Determination of minimum inhibitory concentrations and minimum bactericidal concentrations of compounds against A. jandaei and A. hydrophila, with compounds B, C, D, and E showing potential antimicrobial activity. - Evaluation of toxicity risks

1. XXVII Brazilian Congress in BiomedicalEngineering(CBEB 2020)
October26-30 2020 Vitoria(Brazil)
Pesquisas com maleimidas
contra bactérias de tambaqui

2. MBR Technology LTDA
Dra. Claire Juliana Francisco

3. Disciplina Procad 2020
✓Maleimidas

4. (Peixe BR, 2020)
4,9%
Produção aquícola
brasileira: 758.000 t
Tambaqui (Colossoma
macropomum);
Matrinxã (Brycon
amazonicus);
Pirarucu
(Arapaima gigas).
20.596 t
. AMAZONAS
Disciplina Procad 2020

5. Desenvolvimento
de Novos
Farmacos
Doenças
Infecciosas
Aquicultura
Disciplina Procad 2020

6. Disciplina Procad 2020

7. • Subclasses de Imidas
• Antividade
antimicrobiana
Disciplina Procad 2020

8. A B C
Disciplina Procad 2020

9. Disciplina Procad 2020

10. Disciplina Procad 2020

11. Sintese

12. XXVII Brazilian Congressin BiomedicalEngineering(CBEB 2020)
October26-30 2020 Vitoria(Brazil)
Caracterizaçao
Methyl 4-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1- yl) benzoate.
HPLC-Exact Mass calculated for
C12H10NO4 232.0604 (M + 1),
and determined at 232.0609.
68%
Disciplina Procad 2020

13. XXVII Brazilian Congressin BiomedicalEngineering(CBEB 2020)
October26-30 2020 Vitoria(Brazil)
Caracterizaçao
Spectrum of RMN 1H, in CDCl3 a 500 MHz 4-
(2,5-dioxo-2,5- dihydro-1H-pyrrol-1-yl) benzoate.
Spectrum of RMN 13C, in CDCl3 a 125 MHz 4-(2,5-
dioxo-2,5- dihydro-1H-pyrrol-1-yl) benzoate.
Disciplina Procad 2020

14. PESQUISA
Disciplina Procad 2020

15. Disciplina Procad 2020
PESQUISA

16. MALDI-TOF
Biologia Molecular
Disciplina Procad 2020
PESQUISA

17. A B
C
D
E F
Disciplina Procad 2020
PESQUISA

18. (MUT)=mutagênico;
(TUM)=tumorogenico;
(IRRI)=Irritante;
(REP)=Interferencia na
reprodução);
(PM)=peso molecular;
(cLogP)=hidrofilicidade;
(SOL)=solubilidade;
(DL)=druglikeness
(DS)= drugscore
Disciplina Procad 2020
PESQUISA

19. RESULTADOS
Concentração Mínima Inibitória (CIM) e Concentração Mínima Bactericida (CBM)
Molécula
A. jandaei (µg/mL) A. hydrophila (µg/mL)
CIM CBM CBM/CIM CIM CBM CBM/CIM
A >1000 - - >1000 - -
B 62,5 62,5 1 125 125 1
C 62,5 62,5 1 62,5 62,5 1
D 62,5 62,5 1 250 250 1
E 166,25 166,25 1 166,25 166,25 1
F 500 500 1 500 500 1
G >1000 - - >1000 - -
Disciplina Procad 2020

20. Riscos de toxicidade Farmacocinético Drug
Fórmula química MUT TUM IRRI REP PM cLogP SOL DL DS
(A) C28H43NO2 425,0 8,77 -6,9 -24,9 0,12
(B) C12H12N202 216,0 0,64 -2,11 -13,02 0,17
(C) C12H9NO2 199,0 1,45 -2,88 -9,86 0,22
(D) C10H6ClNO2 207,0 1,35 -2,81 -6,31 0,28
(E) C10H7NO2 173,0 0,74 -2,07 -4,49 0,29
(F) C11H7NO4 217,0 0,23 -2,09 -8,11 0,48
(G) C15H15NO4 273,0 1,8 -3,0 -30,33 0,27
Oxitetraciclina 460,0 2,18 -1,45 5,36 0,8
Florfenicol 357,0 0,2 -2,91 2,55 0,11
Avaliação da citotoxicidade
Disciplina Procad 2020
RESULTADOS

21. Disciplina Procad 2020

22. XXVII Brazilian Congress in BiomedicalEngineering(CBEB 2020)
October26-30 2020 Vitoria(Brazil)
Dra. Claire JulianaFrancisco
email: aquicultura2005@hotmail.com
Obrigada