1) LAR is a receptor for chondroitin sulfate proteoglycans (CSPGs), which are inhibitory to axon growth in the central nervous system.
2) CSPGs bind to LAR with high affinity and stimulate its phosphatase activity. Blocking LAR reverses CSPG-mediated growth inhibition.
3) In mouse models of spinal cord injury, peptides targeting LAR promote axon growth, functional recovery, and behavioral improvements by overcoming the inhibitory effects of CSPGs. LAR blockade may be a promising therapeutic approach for central nervous system axon injury.
Group presentation that contains:
- outlining the basics of translation
- experimental evidence that shows proteins from synonymous mRNA sequences differ
- hypothesis for how synonymous codons effect the resulting protein structure
- the methodology I use to test for the conservation of codon choice within related proteins
Group presentation that contains:
- outlining the basics of translation
- experimental evidence that shows proteins from synonymous mRNA sequences differ
- hypothesis for how synonymous codons effect the resulting protein structure
- the methodology I use to test for the conservation of codon choice within related proteins
Tumor suppression and inflammation: controlling the senescence associated se...adamfreund
This is the powerpoint presentation from a talk I gave at a conference in October, 2009. It will be hard to follow without the spoken part, but it will hopefully give anyone who is interested a brief introduction to my thesis research.
Do plants contain typical GPCRs?” How is G-protein signaling operating in plants.
G-proteins are universal signal transducers mediating many cellular responses. In animal systems the G-protein signaling cycle is activated by seven transmembrane-spanning G-protein coupled receptors (or GPCRs, popularly known as “serpentine receptors”). Whether typical G protein-coupled receptors (GPCRs) exist in plants or not is a fundamental question. In contrast to the animal system, the existence of these types of receptors in plants still remains controversial. While in animals ligand binding causes a change in receptor conformation that activate a particular G Protein, in plants, such mechanism is unknown. In fact, it is considered that the plants G-Proteins are self-activating. The G Proteins have their respective GPCRs in animal system. A lot of information is already accumulated in animal system and hence the animal GPCRs are considered “canonical.” Thus, from the very beginning, plant G-proteins have been compared with the animal counterparts and studied as an extrapolation of the animal model. This presentation provides an insight into the molecular mechanisms of G Protein activation in plants as well as whether “canonical” GPCRs are present in any plant species or not.
I presented two fascinating stories where Molecular Dynamics simulations contributed to enhancing our understanding of immunodeficiencies. In one of the projects, the treatment of patients could be improved. These slides were presented at the Basler Modeller Stammtisch, 26.02.2021
Tumor suppression and inflammation: controlling the senescence associated se...adamfreund
This is the powerpoint presentation from a talk I gave at a conference in October, 2009. It will be hard to follow without the spoken part, but it will hopefully give anyone who is interested a brief introduction to my thesis research.
Do plants contain typical GPCRs?” How is G-protein signaling operating in plants.
G-proteins are universal signal transducers mediating many cellular responses. In animal systems the G-protein signaling cycle is activated by seven transmembrane-spanning G-protein coupled receptors (or GPCRs, popularly known as “serpentine receptors”). Whether typical G protein-coupled receptors (GPCRs) exist in plants or not is a fundamental question. In contrast to the animal system, the existence of these types of receptors in plants still remains controversial. While in animals ligand binding causes a change in receptor conformation that activate a particular G Protein, in plants, such mechanism is unknown. In fact, it is considered that the plants G-Proteins are self-activating. The G Proteins have their respective GPCRs in animal system. A lot of information is already accumulated in animal system and hence the animal GPCRs are considered “canonical.” Thus, from the very beginning, plant G-proteins have been compared with the animal counterparts and studied as an extrapolation of the animal model. This presentation provides an insight into the molecular mechanisms of G Protein activation in plants as well as whether “canonical” GPCRs are present in any plant species or not.
I presented two fascinating stories where Molecular Dynamics simulations contributed to enhancing our understanding of immunodeficiencies. In one of the projects, the treatment of patients could be improved. These slides were presented at the Basler Modeller Stammtisch, 26.02.2021
Oncology: Spatial Localization of Ras proteinsNachiket Vartak
This is a presentation of work done at the MPI Dortmund from 2008-2013 on the mechanism through with localization of the Ras protein in generated in cells. It presents the inhibiton Palmostatin-B, which inhibits this mechanism, leading to reveral of oncogenic signaling and cancerous phenotypes.
Dr. kristen park kcnq2 Cure professional track - learn more at kcnq2cure.orgscottyandjim
Dr. Kristen Park speaking at 2014 Denver KCNQ2 Cure summit professionals track at Children's Hospital of Colorado. More information at www.kcnq2cure.org
Creative Biolabs' dedicated team of neuroscience CRO scientists want the same thing as you do: to find a cure for the devastating diseases of the central nervous system.
https://neuros.creative-biolabs.com/
GDG Cloud Southlake #33: Boule & Rebala: Effective AppSec in SDLC using Deplo...James Anderson
Effective Application Security in Software Delivery lifecycle using Deployment Firewall and DBOM
The modern software delivery process (or the CI/CD process) includes many tools, distributed teams, open-source code, and cloud platforms. Constant focus on speed to release software to market, along with the traditional slow and manual security checks has caused gaps in continuous security as an important piece in the software supply chain. Today organizations feel more susceptible to external and internal cyber threats due to the vast attack surface in their applications supply chain and the lack of end-to-end governance and risk management.
The software team must secure its software delivery process to avoid vulnerability and security breaches. This needs to be achieved with existing tool chains and without extensive rework of the delivery processes. This talk will present strategies and techniques for providing visibility into the true risk of the existing vulnerabilities, preventing the introduction of security issues in the software, resolving vulnerabilities in production environments quickly, and capturing the deployment bill of materials (DBOM).
Speakers:
Bob Boule
Robert Boule is a technology enthusiast with PASSION for technology and making things work along with a knack for helping others understand how things work. He comes with around 20 years of solution engineering experience in application security, software continuous delivery, and SaaS platforms. He is known for his dynamic presentations in CI/CD and application security integrated in software delivery lifecycle.
Gopinath Rebala
Gopinath Rebala is the CTO of OpsMx, where he has overall responsibility for the machine learning and data processing architectures for Secure Software Delivery. Gopi also has a strong connection with our customers, leading design and architecture for strategic implementations. Gopi is a frequent speaker and well-known leader in continuous delivery and integrating security into software delivery.
Kubernetes & AI - Beauty and the Beast !?! @KCD Istanbul 2024Tobias Schneck
As AI technology is pushing into IT I was wondering myself, as an “infrastructure container kubernetes guy”, how get this fancy AI technology get managed from an infrastructure operational view? Is it possible to apply our lovely cloud native principals as well? What benefit’s both technologies could bring to each other?
Let me take this questions and provide you a short journey through existing deployment models and use cases for AI software. On practical examples, we discuss what cloud/on-premise strategy we may need for applying it to our own infrastructure to get it to work from an enterprise perspective. I want to give an overview about infrastructure requirements and technologies, what could be beneficial or limiting your AI use cases in an enterprise environment. An interactive Demo will give you some insides, what approaches I got already working for real.
Epistemic Interaction - tuning interfaces to provide information for AI supportAlan Dix
Paper presented at SYNERGY workshop at AVI 2024, Genoa, Italy. 3rd June 2024
https://alandix.com/academic/papers/synergy2024-epistemic/
As machine learning integrates deeper into human-computer interactions, the concept of epistemic interaction emerges, aiming to refine these interactions to enhance system adaptability. This approach encourages minor, intentional adjustments in user behaviour to enrich the data available for system learning. This paper introduces epistemic interaction within the context of human-system communication, illustrating how deliberate interaction design can improve system understanding and adaptation. Through concrete examples, we demonstrate the potential of epistemic interaction to significantly advance human-computer interaction by leveraging intuitive human communication strategies to inform system design and functionality, offering a novel pathway for enriching user-system engagements.
Dev Dives: Train smarter, not harder – active learning and UiPath LLMs for do...UiPathCommunity
💥 Speed, accuracy, and scaling – discover the superpowers of GenAI in action with UiPath Document Understanding and Communications Mining™:
See how to accelerate model training and optimize model performance with active learning
Learn about the latest enhancements to out-of-the-box document processing – with little to no training required
Get an exclusive demo of the new family of UiPath LLMs – GenAI models specialized for processing different types of documents and messages
This is a hands-on session specifically designed for automation developers and AI enthusiasts seeking to enhance their knowledge in leveraging the latest intelligent document processing capabilities offered by UiPath.
Speakers:
👨🏫 Andras Palfi, Senior Product Manager, UiPath
👩🏫 Lenka Dulovicova, Product Program Manager, UiPath
UiPath Test Automation using UiPath Test Suite series, part 3DianaGray10
Welcome to UiPath Test Automation using UiPath Test Suite series part 3. In this session, we will cover desktop automation along with UI automation.
Topics covered:
UI automation Introduction,
UI automation Sample
Desktop automation flow
Pradeep Chinnala, Senior Consultant Automation Developer @WonderBotz and UiPath MVP
Deepak Rai, Automation Practice Lead, Boundaryless Group and UiPath MVP
LF Energy Webinar: Electrical Grid Modelling and Simulation Through PowSyBl -...DanBrown980551
Do you want to learn how to model and simulate an electrical network from scratch in under an hour?
Then welcome to this PowSyBl workshop, hosted by Rte, the French Transmission System Operator (TSO)!
During the webinar, you will discover the PowSyBl ecosystem as well as handle and study an electrical network through an interactive Python notebook.
PowSyBl is an open source project hosted by LF Energy, which offers a comprehensive set of features for electrical grid modelling and simulation. Among other advanced features, PowSyBl provides:
- A fully editable and extendable library for grid component modelling;
- Visualization tools to display your network;
- Grid simulation tools, such as power flows, security analyses (with or without remedial actions) and sensitivity analyses;
The framework is mostly written in Java, with a Python binding so that Python developers can access PowSyBl functionalities as well.
What you will learn during the webinar:
- For beginners: discover PowSyBl's functionalities through a quick general presentation and the notebook, without needing any expert coding skills;
- For advanced developers: master the skills to efficiently apply PowSyBl functionalities to your real-world scenarios.
Key Trends Shaping the Future of Infrastructure.pdfCheryl Hung
Keynote at DIGIT West Expo, Glasgow on 29 May 2024.
Cheryl Hung, ochery.com
Sr Director, Infrastructure Ecosystem, Arm.
The key trends across hardware, cloud and open-source; exploring how these areas are likely to mature and develop over the short and long-term, and then considering how organisations can position themselves to adapt and thrive.
Securing your Kubernetes cluster_ a step-by-step guide to success !KatiaHIMEUR1
Today, after several years of existence, an extremely active community and an ultra-dynamic ecosystem, Kubernetes has established itself as the de facto standard in container orchestration. Thanks to a wide range of managed services, it has never been so easy to set up a ready-to-use Kubernetes cluster.
However, this ease of use means that the subject of security in Kubernetes is often left for later, or even neglected. This exposes companies to significant risks.
In this talk, I'll show you step-by-step how to secure your Kubernetes cluster for greater peace of mind and reliability.
AI for Every Business: Unlocking Your Product's Universal Potential by VP of ...
Li, Shuxin
1. LAR is a functional receptor for CSPG axon growth inhibitors Shuxin Li Department of Neurology and Neuroscience Program UT Southwestern Medical Center at Dallas
2. Upregulation of CSPGs after the CNS injury Core protein GAG chains Exp Neurol. 2003;182(2):399-411 Both GAG and core protein are inhibitory GAG: glycosaminoglycan KSPG: Keratan
3. CSPGs contribute to axon growth failure in the CNS Nature 416: 636-640; 2002 Molecular mechanisms for CSPG inhibitions • Negative charges of sugar chains and interrupting cell adhesion. • Blocking functions of positive molecules: laminin & integrins. • As receptor for Sema 5A. • Binding to specific receptors for CSPGs. RPTPσ as CSPG receptor (Science, 2009, 326:592-596)
4. LAR and LAR subfamily of proteins • The heparan sulfate proteoglycansyndecan is an in vivo ligand for the Drosophila LAR receptor tyrosine phosphatase. Current Biology, 2005; 15:1701. • The HSPGs Syndecan and Dallylike Bind the Receptor Phosphatase LAR and Exert Distinct Effects on Synaptic Development. Neuron 49, 517–531, 2006. ST3: mutant Transmembrane proteins Extracell: Ig & fibronectin type IIIdomains Intracell: D1 and D2 domains; D1-activity; D2-regulating ligandbinding Major members: LAR, RPTPσ & RPTPδ Physical ligands and functions are unclear.
6. CSPGs bind to LAR in COS-7 cells with high affinity • Incubate cells with CSPGs 7.5 ug/ml. • Stained CSPG signal with Ab. • Delta D2: deletion in D2 domain: regulating ligand binding. • C1522S: mutant in D1 domain: regulating activity, not binding.
7. CSPG & chondroitin sulfate enhance activity of LAR phosphatasein COS-7 cells • COS7 transfection: 2 days • Cell lysates treated with CSPG or CS • LAR activity by measuring phasphatase-driven generation of phosphate using a PTP kit
8. LAR deletion overcomes neurite growth inhibition of CSPGs 1 day DRG neuronal cultures from LAR KO mice
9. LAR-targeting peptides overcome CSPG inhibition • The extracellular LAR peptide (ELP): 37 residues, corresponding to the fifth LAR fibronectin type III (FN-III) domain. • The intracellular LAR peptide (ILP): 35 amino acids, including 24 residues derived from sequence located between the membrane proximal region and the D1catalytic domain, and membrane-penetrating sequence (TAT) domain.
10. Systemic treatments with ELP/ILP in SCI mice Hemisection at T7 Dorsal Lesioned Adult mouse Ventral Peptide application: • Starting 2 days post-SCI • Dose: 140 (ELP) or 75 (ILP) mg/Kg/day • Infusion time: 10 days Tissue processing Lesion 7 mm 4mm 7 mm 4mm R C Cross Parasagittal Cross
11. ELP and ILP stimulate 5-HT axonal growth in SCI mice
12. ELP and ILP stimulate 5-HT axonal growth in SCI mice: Camera Lucida drawing
13. ELP and ILP stimulate 5-HT axonal growth in SCI mice 5 weeks post-SCI
14. ELP and ILP promote behavioral recovery in SCI mice
15. Conclusions CSPGs bind LAR and interact with LAR with high affinity. CSPG stimulates LAR phosphatase activity in vitro. Transgenic/pharmacological blockade of LAR reverses CSPG-mediated growth inhibition. 4. LAR blockade promotes axon growth and functional recovery in adult SCI mice. CSPG LAR Conclusions: LAR is an important receptor for CSPG inhibitors. LAR blockade has a great therapeutic potential for CNS axon injury disorders. LAR peptides may become a feasible treatment for axon injury patients.
16. Acknowledgements Neuronal cultures neurite/axon growth assay and quantification mouse breeding & genotyping LAR mutation Mikey Hoang Daniel Fisher John Dill Ankur Patel Zhenze Zhao Xiao-Li Yang Hui Li Bin Xing In vivo axon regeneration, behaviors and histology Frank Longo (Stanford U): LAR KO & over-expression mice Morgan Sheng (MIT): LAR plasmids Jerry Silver (Case Western Reserve U): confirm LAR peptides using CSPG spot assay Grant supports: ParalyzedVeterans of America, NIH (1R21NS066114-01A1), Morton Cure Paralysis Fund and Christopher and Dana Reeve Foundation.