Instrumental Project

1. Spectroflorimetry
applications
By Shaden Mohamed & Yahia khaled

2. Introduction
Spectrofluorimetry is the study of emitted fluorescence from chemical compounds.
Spectrofluorometer is an excellent diagnostic and research tool which is immensely
utilized in various fields of science for its high sensitivity and specificity.
It utilizes a light source. Examples include: xenon lamps, mercury vapour, laser and
led lamps.
Light passes through a monochromator, to specify the specific wavelength needed and
is absorbed by the sample. The atoms of sample are then excited and emit
fluorescence to reach ground state.
Analyzer monochromator filters out stray light and the intensity of transmitted
light is measured by a detector.
Fluorescence wavelength is specific to molecules. Light intesnity is indicative of
quanitity of analyte.

3. Study of marine pollutants
Some hydrocarbons obtain the ability to fluoresce.
This ability allows for determination of pollutants in river
and lake waters, qualitatively and quantitatively.

4. Accurate Glucose determination
Determination of glucose is crucial for clinical
investigations.
Utilization of bio-stabilized gold (Au) nanoclusters as
fluorescence probe.

5. Analysis of pharmaceutical drugs
Spectrofluorimetry is used in quantitative determination of
medications such as morphine and riboflavin.
This is especially important in the pharmaceutical industry
as it allows for quality control of production of these
drugs.

6. Analysis of Antibody and ligand binding characteristics
A novel treatment for cocaine abuse employs the use of
monoclonal anti-cocaine antibodies.
It is an inexpensive and reproducible method of detection of
ligand binding affinity.

7. Bioscience
In the biosciences, one of the most frequent applications of
fluorescence spectroscopy is the high precision
quantification of DNA and RNA. An extrinsic fluorophore
(often ethidium bromide) is added to a DNA sample and the
sample is loaded into a fluorescence spectrometer to obtain
a reading of the sample’s concentration. Another modern
application is SMRT (single molecule real-time) DNA
sequencing. In its ability to produce long read single
molecules with high accuracy, it is predicted to be central
to the next genetic diagnostic revolution

8. Agricultural
In agriculture, spectroscopic techniques are also widely
applied for instance in the identification of different crop
varieties. Laser-induced fluorescent emission technique
(LIFS) is an excellent tool used to identify citrus seedling
varieties .
Likewise, total luminescence spectroscopy can be used by tea
manufacturers as a quick, affordable and objective
alternative to employing trained tea tasters, to
discriminate between similar types of tea

9. Some instruments used in Spectroflorimetry
1-Mira and Misa handheld Raman and SERS analyzers
● Metrohm Instant Raman Analyzers (Mira) and Metrohm Instant SERS Analyzers (Misa) are
robust and reliable portable spectrometers for material identification and
verification.
● These instruments combine ease of use with maximum safety and provide results within
seconds. Using ORS, Mira systems yield highly reliable results, giving you more
confidence in your measurements.
● True single-hand operation:
Small and light-weight battery-operated Raman spectrometers
● Quick and reliable results:
Reliable identification of materials in just seconds.
● ORS (Orbital Raster Scan) technology
for accurate, reproducible, and reliable results
Non-destructive and versatile:
Multiple sampling attachments for any type of analysis

10. .2-Vis-NIR Spectroscopy Lab
Analyzers
Metrohm NIRS lab analyzers enable you
to perform routine analysis quickly and
with confidence – without requiring
sample preparation or additional
reagents and yielding results in less
than a minute.
Combining visible (Vis) and near-
infrared (NIR) spectroscopy, these
analyzers are capable of performing
qualitative analysis of various
materials and quantitative analysis of
a number of physical and chemical
parameters in one run.
3-B&W Tek handheld Raman analyzers
Handheld Raman spectroscopy has contributed to
increased quality control and sample screening
through cost-effective, easy-to-use analyzers
that can be used at the point of need.
Raman spectroscopy can be used for direct
sample analysis typically without
preprocessing or preparation. Raman has the
unique capability of being able to test a
sample directly through transparent packing
material like glass or plastic, making it a
fast, easy method that can be used for
material identification and analysis wherever
it is needed. With their portability and
intuitive operation, handheld Raman
spectrometers considerably increase efficiency
by eliminating the need for additional space
and wet chemistry.

11. References
1-Kirley, T., Norman, A., & Wetzel, H. (2019, October 18). A novel differential scanning fluorimetry analysis of a humanized anti-
cocaine mab and its ligand binding characteristics. Retrieved May 09, 2021, from
https://www.sciencedirect.com/science/article/pii/S0022175919303035
2-https://conductscience.com/fluorescence-spectrophotometry-principles-and-applications/
3-https://www.metrohm.com/en-ae/products-overview/spectroscopy/?gclid=Cj0KCQjws-OEBhCkARIsAPhOkIbZjpSLFoc_33xCv1XjQSkeQjylB-
Z0129YXS0rbH-06IeDgt9uNhoaAlRqEALw_wcB

12. Thank you