Imagine a yeast-infecting virus that uses DNA replication proteins o.pdf

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Imagine a yeast-infecting virus that uses DNA replication proteins of a host yeast cell to copy the viral genome. The virus helps itself( and also sometimes harms the yeast) by making a very specific change to the yeast in the G1-to S-phase checkpoint system. What is the mutation and how is it helpful? Solution Answer) G1 to S phase checkpoint is the first checkpoint in cell cycle. there are certain transcriotional repressors such as rb, p130 and p107 called as pocket proteins. increased level of cyclin proteins remove this transcription repressor and G1 to S phase transition occurs. cyclin dependant complexes are inhibited by P21 gene which inturn regulated by P53 gene. these are different possible point of mutations which can disturb the G1 to S phase transition. but, the most specific mutation will be either in gene P21 or gene P53. in case of mutated P21, no cyclin protein will be inhibited and increased cyclin proteins synthesis would promote G1 to S phase transition via uncoupling transcription repressors and an unconditional and unregulated DNA replication can be achieved in such case..

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