PCR explained in simple terms - A T G & C of PCR - Question and answers PCRajithnandanam
www.technologyinscience.blogspot.com
PCR - polymerase chain reaction explained in simple question answer format. Type in your doubts on PCR in the comment.
Transfection is the process of introduction of foreign DNA into the nucleus of eukaryotic cell. The cells which has incorporated exogenous DNA are called transfectants.
There are two types of Transfection possible,
Transient and
Stable Transfection.
In transient Transfection, the foreign DNA will not get incorporated in to the host genome, but genes are expressed for limited period of time (24-96 hrs).
Stable transfectants will have the foreign DNA incorporated into the genome.
Xiaomi is a Chinese electronics company that produces smartphones and other consumer electronics. Their name is pronounced "Shy-ow-mee" with the accent on the second syllable. To pronounce it correctly, break it down into three syllables - "Shy-ow-mee" - with a soft 'i' sound in the first syllable.
Top 15 brands which you may find difficult to Say or pronounce ajithnandanam
15 top brands which you may find difficult to pronounce. How to Pronounce or Say HERMES, GUCCI, MIELE, PORSCHE, HOEGAARDEN, FAGE, ZAGAT, STILA, STELLA ARTOIS, GIVENCHY, GUERLAIN, KINERASE, ADIDAS, SAUCONY, BOEHRINGER INGELHEIM
Yeast two hybrid system for Protein Protein Interaction Studiesajithnandanam
Yeast Two Hybrid system uses a reporter gene to detect the interaction of pair of proteins inside the yeast cell nucleus. In the yeast Two Hybrid System, The interaction of target protein to the protein will bring together transcriptional activator, which then switches on the expression of reporter gene.
Aptamers - New Class of Oligonucleotide for Therapeutic and Diagnostic Useajithnandanam
http://technologyinscience.blogspot.com/2014/01/aptamers-new-class-of-oligonucleotide.html
Aptamers are single-stranded RNA or DNA oligonucleotides 15 to 60 base in length that bind with high affinity to specific molecular targets; most aptamers to proteins bind with Kds (equilibrium constant) in the range of 1 pM to 1 nM similar to monoclonal antibodies. These nucleic acid ligands bind to nucleic acid, proteins, small organic compounds, and even entire organisms. Aptamers have many potential uses in intracellular processes studies, medicine and technology. Aptamers are often identified using a technique called SELEX (Systematic Evolution of Ligands by EXponential enrichment). By this techniques aptamers(oligos) having high affinity and specificity to the target is isolated from the sequence pool after several rounds of selection.
Nucleic Acid Quantification Methods - DNA / RNA Quantificationajithnandanam
Nucleic acids are quantified to check the concentration and purity of DNA/RNA present in the solution mixture.it is important to know the concentration and purity of the nucleic acid for the use in further applications like PCR, restriction digestion etc. Spectrophotometric analysis is the most commonly used method of quantifying DNA, agarose gel electrophoresis can also be used to analyse the DNA sample for purity.
Recombinant protein expression in E.coliajithnandanam
Recombinant Protein expression in E.coli, Best suitable strains for protein expression, advantages of using E.coli for choosing the host for protein expression
PCR explained in simple terms - A T G & C of PCR - Question and answers PCRajithnandanam
www.technologyinscience.blogspot.com
PCR - polymerase chain reaction explained in simple question answer format. Type in your doubts on PCR in the comment.
Transfection is the process of introduction of foreign DNA into the nucleus of eukaryotic cell. The cells which has incorporated exogenous DNA are called transfectants.
There are two types of Transfection possible,
Transient and
Stable Transfection.
In transient Transfection, the foreign DNA will not get incorporated in to the host genome, but genes are expressed for limited period of time (24-96 hrs).
Stable transfectants will have the foreign DNA incorporated into the genome.
Xiaomi is a Chinese electronics company that produces smartphones and other consumer electronics. Their name is pronounced "Shy-ow-mee" with the accent on the second syllable. To pronounce it correctly, break it down into three syllables - "Shy-ow-mee" - with a soft 'i' sound in the first syllable.
Top 15 brands which you may find difficult to Say or pronounce ajithnandanam
15 top brands which you may find difficult to pronounce. How to Pronounce or Say HERMES, GUCCI, MIELE, PORSCHE, HOEGAARDEN, FAGE, ZAGAT, STILA, STELLA ARTOIS, GIVENCHY, GUERLAIN, KINERASE, ADIDAS, SAUCONY, BOEHRINGER INGELHEIM
Yeast two hybrid system for Protein Protein Interaction Studiesajithnandanam
Yeast Two Hybrid system uses a reporter gene to detect the interaction of pair of proteins inside the yeast cell nucleus. In the yeast Two Hybrid System, The interaction of target protein to the protein will bring together transcriptional activator, which then switches on the expression of reporter gene.
Aptamers - New Class of Oligonucleotide for Therapeutic and Diagnostic Useajithnandanam
http://technologyinscience.blogspot.com/2014/01/aptamers-new-class-of-oligonucleotide.html
Aptamers are single-stranded RNA or DNA oligonucleotides 15 to 60 base in length that bind with high affinity to specific molecular targets; most aptamers to proteins bind with Kds (equilibrium constant) in the range of 1 pM to 1 nM similar to monoclonal antibodies. These nucleic acid ligands bind to nucleic acid, proteins, small organic compounds, and even entire organisms. Aptamers have many potential uses in intracellular processes studies, medicine and technology. Aptamers are often identified using a technique called SELEX (Systematic Evolution of Ligands by EXponential enrichment). By this techniques aptamers(oligos) having high affinity and specificity to the target is isolated from the sequence pool after several rounds of selection.
Nucleic Acid Quantification Methods - DNA / RNA Quantificationajithnandanam
Nucleic acids are quantified to check the concentration and purity of DNA/RNA present in the solution mixture.it is important to know the concentration and purity of the nucleic acid for the use in further applications like PCR, restriction digestion etc. Spectrophotometric analysis is the most commonly used method of quantifying DNA, agarose gel electrophoresis can also be used to analyse the DNA sample for purity.
Recombinant protein expression in E.coliajithnandanam
Recombinant Protein expression in E.coli, Best suitable strains for protein expression, advantages of using E.coli for choosing the host for protein expression
Mechanism of interaction of Ethidium Bromide (EtBr) with DNAajithnandanam
Ethidium Bromide contains tricyclic phenanthridine ring system that is able to interact with stacked base pairs of double stranded DNA. Ethidium is capable of forming close van der Walls contacts with the base pairs and due to that it can bind to the hydrophobic interior of the DNA molecule. The peripheral phenyl and ethyl groups projects into the major groove of DNA helix.
This document summarizes the specifications of 5 top smartphones from 2014: the HTC One M8, LG G2, Samsung Galaxy S5, Sony Xperia Z2, and Google Nexus 5. All five phones have quad-core processors between 2.2-2.5 GHz, 2-3 GB of RAM, Android 4.4 operating systems, and high resolution screens between 4.7-5.2 inches. They vary in camera resolution from 8-20 megapixels on the rear and 2-5 megapixels on the front. Storage ranges from 16-32GB with some supporting microSD expansion. Battery capacities are between 2300-3200mAh.
Top 10 Most Expensive Cars in the Worldajithnandanam
The document lists the top 10 most expensive cars in the world, ranging in price from 12 crore for the Bugatti Veyron down to 2.3 crore for the Lamborghini Murcielago. The Bugatti Veyron is the most expensive at 12 crore, followed by the Rolls Royce Phantom at 4 crore, and the Aston Martin Vanquish at 3.8 crore.
This document discusses using the ClusPro protein-protein docking software. It explains entering receptor and ligand IDs to perform docking simulations, then provides status updates and details during the docking process. Finally, it discusses the docking results output from ClusPro simulations.
How to delete email in lumia 625, lumia 520, lumia 720, lumia 920, lumia 925,...ajithnandanam
To delete, move, or flag an email on a Lumia phone running Windows 8, open the email app and press and hold your finger on the email until a menu pops up offering options to delete, move to a folder, or flag the email.
The heart of the fermentation or bioprocess technology is the Fermentor or Bioreactor. A bioreactor is basically a device in which the organisms are cultivated to form the desired products. it is a containment system designed to give right environment for optimal growth and metabolic activity of the organism.
A fermentor usually refers to the containment system for the cultivation of prokaryotic cells, while a bioreactor grows the eukaryotic cells (mammalian, insect cells, etc).
http://technologyinscience.blogspot.com/2014/02/ethanol-precipitation-of-dna-principle.html
Ethanol Precipitation is a method for purifying or concentrating DNA/RNA from aqueous solutions using Ethanol as anti-solvent.The precipitation is accompanied by the addition of salt and ethanol to a solution containing DNA or RNA.In presence of monovalent cations (eg: Na+) ethanol efficiently precipitates nucleic acids. The precipitate can be collected by centrifugation.
Saving involves depositing cash in a low-risk, low-return manner in savings accounts, fixed deposits, or government funds. Investing means purchasing higher-risk assets like stocks, bonds, mutual funds, or real estate that offer potential for higher returns. People save or invest for short-term or long-term goals like covering expenses, purchasing large assets, retirement plans, and more.
ELISA Vs ELISPOT - Principle, Procedure, Advantagesajithnandanam
The Enzyme Linked Immunospot (ELISPOT) technique was developed by Cecil Czerkinskdy in 1983. ELISPOT is used for the detection of secreted proteins, such as cytokines and growth factors. ELISPOT is primarily used in immunology research in the following areas:
Oligonucleotide synthesis - Problems and Challengesajithnandanam
The oligonucleotides are synthesized on solid supports from the 3’-end and the first monomer at this end is normally attached to a CPG(Controlled Pore Glass) or Polystyrene (PS).
AIDS is the Deadliest Pandemic in humans and it is caused by a RNA virus called Human Immunodeficiency Virus (HIV).AIDS is The Last Stage of HIV Infection.
• First Identified By Dr.Luc Montagnier & His Collegues.
• It is Estimated That 8-10 Millions Adults & 1 Million Chliderns World Wide Are Infected With HIV Virus.
• AIDS is one of The Sexually Transmitted Disease.
Immunoprecipitation: Procedure, Analysis and Applicationsajithnandanam
Immunoprecipitation is a precipitaion technique which allows the isolation of protein or protein complex from biological samples.
Incubate sample with antibody against protein of interest.
Separate antibody-protein complex from remaining sample
Analysis
Plant hormones (also known as plant growth regulators (PGRs) and phytohormones) are chemicals that regulate a plant's growth. Plant hormones on the other hand, are not like animal hormones, they are often not transported to other parts of the plant and production is not limited to specific locations. Plants lack tissues or organs specifically for the production of hormones; unlike animals, plants lack glands that produce and secrete hormones to be moved around the body. Plant hormones shape the plant, effecting seed growth, time of flowering, the sex of flowers, its longevity, senescence of leaves and fruits, they affect which tissues grow up and which grow downward, leaf formation and stem growth, fruit development and ripening, and even plant death. Hormones are vital to plant growth and lacking them plants would be mostly a mass of undifferentiated cells.
This presentation was provided by Steph Pollock of The American Psychological Association’s Journals Program, and Damita Snow, of The American Society of Civil Engineers (ASCE), for the initial session of NISO's 2024 Training Series "DEIA in the Scholarly Landscape." Session One: 'Setting Expectations: a DEIA Primer,' was held June 6, 2024.
Mechanism of interaction of Ethidium Bromide (EtBr) with DNAajithnandanam
Ethidium Bromide contains tricyclic phenanthridine ring system that is able to interact with stacked base pairs of double stranded DNA. Ethidium is capable of forming close van der Walls contacts with the base pairs and due to that it can bind to the hydrophobic interior of the DNA molecule. The peripheral phenyl and ethyl groups projects into the major groove of DNA helix.
This document summarizes the specifications of 5 top smartphones from 2014: the HTC One M8, LG G2, Samsung Galaxy S5, Sony Xperia Z2, and Google Nexus 5. All five phones have quad-core processors between 2.2-2.5 GHz, 2-3 GB of RAM, Android 4.4 operating systems, and high resolution screens between 4.7-5.2 inches. They vary in camera resolution from 8-20 megapixels on the rear and 2-5 megapixels on the front. Storage ranges from 16-32GB with some supporting microSD expansion. Battery capacities are between 2300-3200mAh.
Top 10 Most Expensive Cars in the Worldajithnandanam
The document lists the top 10 most expensive cars in the world, ranging in price from 12 crore for the Bugatti Veyron down to 2.3 crore for the Lamborghini Murcielago. The Bugatti Veyron is the most expensive at 12 crore, followed by the Rolls Royce Phantom at 4 crore, and the Aston Martin Vanquish at 3.8 crore.
This document discusses using the ClusPro protein-protein docking software. It explains entering receptor and ligand IDs to perform docking simulations, then provides status updates and details during the docking process. Finally, it discusses the docking results output from ClusPro simulations.
How to delete email in lumia 625, lumia 520, lumia 720, lumia 920, lumia 925,...ajithnandanam
To delete, move, or flag an email on a Lumia phone running Windows 8, open the email app and press and hold your finger on the email until a menu pops up offering options to delete, move to a folder, or flag the email.
The heart of the fermentation or bioprocess technology is the Fermentor or Bioreactor. A bioreactor is basically a device in which the organisms are cultivated to form the desired products. it is a containment system designed to give right environment for optimal growth and metabolic activity of the organism.
A fermentor usually refers to the containment system for the cultivation of prokaryotic cells, while a bioreactor grows the eukaryotic cells (mammalian, insect cells, etc).
http://technologyinscience.blogspot.com/2014/02/ethanol-precipitation-of-dna-principle.html
Ethanol Precipitation is a method for purifying or concentrating DNA/RNA from aqueous solutions using Ethanol as anti-solvent.The precipitation is accompanied by the addition of salt and ethanol to a solution containing DNA or RNA.In presence of monovalent cations (eg: Na+) ethanol efficiently precipitates nucleic acids. The precipitate can be collected by centrifugation.
Saving involves depositing cash in a low-risk, low-return manner in savings accounts, fixed deposits, or government funds. Investing means purchasing higher-risk assets like stocks, bonds, mutual funds, or real estate that offer potential for higher returns. People save or invest for short-term or long-term goals like covering expenses, purchasing large assets, retirement plans, and more.
ELISA Vs ELISPOT - Principle, Procedure, Advantagesajithnandanam
The Enzyme Linked Immunospot (ELISPOT) technique was developed by Cecil Czerkinskdy in 1983. ELISPOT is used for the detection of secreted proteins, such as cytokines and growth factors. ELISPOT is primarily used in immunology research in the following areas:
Oligonucleotide synthesis - Problems and Challengesajithnandanam
The oligonucleotides are synthesized on solid supports from the 3’-end and the first monomer at this end is normally attached to a CPG(Controlled Pore Glass) or Polystyrene (PS).
AIDS is the Deadliest Pandemic in humans and it is caused by a RNA virus called Human Immunodeficiency Virus (HIV).AIDS is The Last Stage of HIV Infection.
• First Identified By Dr.Luc Montagnier & His Collegues.
• It is Estimated That 8-10 Millions Adults & 1 Million Chliderns World Wide Are Infected With HIV Virus.
• AIDS is one of The Sexually Transmitted Disease.
Immunoprecipitation: Procedure, Analysis and Applicationsajithnandanam
Immunoprecipitation is a precipitaion technique which allows the isolation of protein or protein complex from biological samples.
Incubate sample with antibody against protein of interest.
Separate antibody-protein complex from remaining sample
Analysis
Plant hormones (also known as plant growth regulators (PGRs) and phytohormones) are chemicals that regulate a plant's growth. Plant hormones on the other hand, are not like animal hormones, they are often not transported to other parts of the plant and production is not limited to specific locations. Plants lack tissues or organs specifically for the production of hormones; unlike animals, plants lack glands that produce and secrete hormones to be moved around the body. Plant hormones shape the plant, effecting seed growth, time of flowering, the sex of flowers, its longevity, senescence of leaves and fruits, they affect which tissues grow up and which grow downward, leaf formation and stem growth, fruit development and ripening, and even plant death. Hormones are vital to plant growth and lacking them plants would be mostly a mass of undifferentiated cells.
This presentation was provided by Steph Pollock of The American Psychological Association’s Journals Program, and Damita Snow, of The American Society of Civil Engineers (ASCE), for the initial session of NISO's 2024 Training Series "DEIA in the Scholarly Landscape." Session One: 'Setting Expectations: a DEIA Primer,' was held June 6, 2024.
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How to Build a Module in Odoo 17 Using the Scaffold MethodCeline George
Odoo provides an option for creating a module by using a single line command. By using this command the user can make a whole structure of a module. It is very easy for a beginner to make a module. There is no need to make each file manually. This slide will show how to create a module using the scaffold method.
A review of the growth of the Israel Genealogy Research Association Database Collection for the last 12 months. Our collection is now passed the 3 million mark and still growing. See which archives have contributed the most. See the different types of records we have, and which years have had records added. You can also see what we have for the future.
LAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UPRAHUL
This Dissertation explores the particular circumstances of Mirzapur, a region located in the
core of India. Mirzapur, with its varied terrains and abundant biodiversity, offers an optimal
environment for investigating the changes in vegetation cover dynamics. Our study utilizes
advanced technologies such as GIS (Geographic Information Systems) and Remote sensing to
analyze the transformations that have taken place over the course of a decade.
The complex relationship between human activities and the environment has been the focus
of extensive research and worry. As the global community grapples with swift urbanization,
population expansion, and economic progress, the effects on natural ecosystems are becoming
more evident. A crucial element of this impact is the alteration of vegetation cover, which plays a
significant role in maintaining the ecological equilibrium of our planet.Land serves as the foundation for all human activities and provides the necessary materials for
these activities. As the most crucial natural resource, its utilization by humans results in different
'Land uses,' which are determined by both human activities and the physical characteristics of the
land.
The utilization of land is impacted by human needs and environmental factors. In countries
like India, rapid population growth and the emphasis on extensive resource exploitation can lead
to significant land degradation, adversely affecting the region's land cover.
Therefore, human intervention has significantly influenced land use patterns over many
centuries, evolving its structure over time and space. In the present era, these changes have
accelerated due to factors such as agriculture and urbanization. Information regarding land use and
cover is essential for various planning and management tasks related to the Earth's surface,
providing crucial environmental data for scientific, resource management, policy purposes, and
diverse human activities.
Accurate understanding of land use and cover is imperative for the development planning
of any area. Consequently, a wide range of professionals, including earth system scientists, land
and water managers, and urban planners, are interested in obtaining data on land use and cover
changes, conversion trends, and other related patterns. The spatial dimensions of land use and
cover support policymakers and scientists in making well-informed decisions, as alterations in
these patterns indicate shifts in economic and social conditions. Monitoring such changes with the
help of Advanced technologies like Remote Sensing and Geographic Information Systems is
crucial for coordinated efforts across different administrative levels. Advanced technologies like
Remote Sensing and Geographic Information Systems
9
Changes in vegetation cover refer to variations in the distribution, composition, and overall
structure of plant communities across different temporal and spatial scales. These changes can
occur natural.
Exploiting Artificial Intelligence for Empowering Researchers and Faculty, In...Dr. Vinod Kumar Kanvaria
Exploiting Artificial Intelligence for Empowering Researchers and Faculty,
International FDP on Fundamentals of Research in Social Sciences
at Integral University, Lucknow, 06.06.2024
By Dr. Vinod Kumar Kanvaria
Strategies for Effective Upskilling is a presentation by Chinwendu Peace in a Your Skill Boost Masterclass organisation by the Excellence Foundation for South Sudan on 08th and 09th June 2024 from 1 PM to 3 PM on each day.
How to Add Chatter in the odoo 17 ERP ModuleCeline George
In Odoo, the chatter is like a chat tool that helps you work together on records. You can leave notes and track things, making it easier to talk with your team and partners. Inside chatter, all communication history, activity, and changes will be displayed.