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Health screening
services
By
Kiran Sharma

12/10/2013

Kiran Sharma, Assistant
Professor

1


Health as a state of complete physical,
mental and social well-being and not
merely the absence of disease or infirmity.



Health is the extent to which an individual
or group is able on one hand, to realize
aspirations and satisfy needs and on the
other hand to change or cope with the
environment

HEALTH
12/10/2013

Kiran Sharma, Assistant
Professor

2


Screening refers to examination of a group of
usually asymptomatic individuals to detect
those with a high probability of having a
given disease, typically by means of an
inexpensive diagnostic test.



Monitoring refers to the act of observation
and in some cases intervention generally
referring to monitoring of a condition after
diagnosis to assess and improve outcomes.

SCREENING
12/10/2013

Kiran Sharma, Assistant
Professor

3


Health screening services are the services
provided by the health care professionals
to screen the health status of individuals
with or without positive sign and
symptoms.

HEALTH SCREENING SERVICES
12/10/2013

Kiran Sharma, Assistant
Professor

4
12/10/2013

Kiran Sharma, Assistant
Professor

5


Early detection can make a difference between
relatively simple courses of treatment or lifethreatening complications



Health screenings save lives by early detection of
conditions such as hypertension or elevated
blood sugar and can help prevent serious
diseases



Many cases of disease exist and remain
undiagnosed that seriously affect our quality of
life. Many of these conditions can be corrected or
improved through a simple course of treatment if
once it has been discovered by health screening

IMPORTANCE OF HEALTH
SCREENING
12/10/2013

Kiran Sharma, Assistant
Professor

6


One of the additional benefits of a health
screen is peace of mind and reassurance.



Health Screening Services contributes to
economic burden of the patient by early
diagnosis and prevention.



For many people, the diagnosis comes when
they make contact with the health system for
other reasons for example when admitted or
visited to hospital with minor illness or
injuries.
The longer, people are undiagnosed, the
more likely it is the complication of the
disease



12/10/2013

Kiran Sharma, Assistant
Professor

7


Health screenings are convenient (readily
available), affordable (inexpensive),
comprehensive, very reliable with few
false negatives and flexible. Also it is
painless, noninvasive and inexpensive
tests.

12/10/2013

Kiran Sharma, Assistant
Professor

8


Based on composition of Health screen, it
is classified into three types. They are



STANDARD HEALTH SCREEN



PREMIUM HEALTH SCREEN



EXECUTIVE HEALTH SCREEN

12/10/2013

Kiran Sharma, Assistant
Professor

9








Consultation with health professional
Blood glucose test (marker for diabetes)
Blood cholesterol test (total Cholesterol level)
Blood pressure measurement and evaluation
Body mass indexing (measures height weight
ratio)
Cardiac risk assessment
Comprehensive urine analysis (tests for
elevation of protein, blood etc in urine)

STANDARD HEALTH SCREEN
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Kiran Sharma, Assistant
Professor

10










Consultation with health professional
Blood glucose test (marker for diabetes)
Blood cholesterol test (total Cholesterol level)
Blood pressure measurement and evaluation
Body mass indexing (measures height weight
ratio)
Cardiac risk assessment
Pulmonary function tests (lung capacity, and
screens for possible disease)
Liver function tests (for abnormal liver function)
Comprehensive urine analysis (tests for protein,
blood in the urine and possible kidney disease
infection)

PREMIUM HEALTH SCREEN
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Kiran Sharma, Assistant
Professor

11
Comprehensive one to one consultation
 Blood glucose test (marker for diabetes)
 Blood cholesterol test (total Cholesterol levels)
 Blood pressure measurement and evaluation
 Body mass indexing (measures height and
weight ratio)
 12 lead ECG (measures the electrical conduction
of the heart)
 Cardiac risk assessment
 Renal profile (blood test to check kidney
functions)


EXECUTIVE HEALTH SCREEN
12/10/2013

Kiran Sharma, Assistant
Professor

12
Bone profile (blood test for measure calcium,
phosphate)
 Full blood count (measures red cells, white cells,
hemoglobin etc.)
 Ferritin blood test (can detect hereditary
conditions such as haemochromatosis)
 Pulmonary function tests (lung capacity, and
screens for possible disease)
 Liver function tests (for abnormal liver function)
 Comprehensive urine analysis (tests for protein,
blood in the urine and possible kidney disease
infection)
 Healthy body fat % range


EXECUTIVE HEALTH SCREEN(contd.)
12/10/2013

Kiran Sharma, Assistant
Professor

13


Blood pressure (BP), sometimes
referred to as arterial blood pressure,
is the pressure exerted by circulating
blood upon the walls of blood vessels



During each heartbeat, blood pressure
varies between a maximum (systolic) and
a minimum (diastolic) pressure

BLOOD PRESSURE
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Kiran Sharma, Assistant
Professor

14
Physiology
There are many physical factors that influence
arterial pressure
Amount of blood
Viscosity of blood
Flow of blood
Elasticity of blood vessels
Flexibility of walls of blood12/10/2013 Kiran Sharma, Assistant
vessels
Professor
15
During exercise
 During tension, fear
 During sleeping
 Excitement


12/10/2013

Kiran Sharma, Assistant
Professor

16
PULSE PRESSURE
average 40 mm Hg
12/10/2013

Kiran Sharma, Assistant
Professor

17


It is alternate expansion and elastic recoil
of an artery with each systole.



Pulse rate 70-90 per minute ( same as
heart rate)

Tachycardia- rapid pulse rate
 Bradycardia – slow pulse rate


PULSE
12/10/2013

Kiran Sharma, Assistant
Professor

18
12/10/2013

Kiran Sharma, Assistant
Professor

19
 HIGH

BLOOD PRESSURE
(hypertension)





It is a condition in which the arteries
have persistently elevated blood
pressure.
Every time the human heart beats, it
pumps blood to the whole body through
the arteries.

12/10/2013

Kiran Sharma, Assistant
Professor

20









Smoking
Obesity or being overweight
Being obese/overweight as a child - a research team
at the Indiana University School of Medicine found
that obese/overweight children are much more likely
to suffer from hypertension during adulthood.
Diabetes
Lack of physical activity
Pregnancy

Causes of hypertension
12/10/2013

Kiran Sharma, Assistant
Professor

21
 High levels
 Insufficient

of salt intake (sodium sensitivity).
calcium, potassium, and magnesium
consumption
 Vitamin D deficiency
 High levels of alcohol consumption
 Stress
 Aging
 Medicines such as birth control pills
 Genetics and a family history of hypertension
 Chronic kidney disease
 Adrenal and thyroid problems or tumors
12/10/2013

Kiran Sharma, Assistant
Professor

22


Hypertension is classified as either primary
(essential) hypertension or secondary
hypertension; about 90–95% of cases are
categorized as "primary hypertension" which
means high blood pressure with no obvious
underlying medical cause.[2] The remaining
5–10% of cases (secondary hypertension)
are caused by other conditions that affect the
kidneys, arteries, heart or endocrine system.

12/10/2013

Kiran Sharma, Assistant
Professor

23


Low blood pressure means that your
blood pressure is lower than normal

Low blood pressure
( hypotension)
12/10/2013

Kiran Sharma, Assistant
Professor

24
Some of the causes of low blood pressure
include:
 Not drinking enough fluids (dehydration).
 Medicines, such as high blood pressure

medicine or other heart medicines.
 Health problems such as thyroid disease, severe
infection, bleeding in the intestines, or heart
problems.
 Trauma, such as major bleeding or severe burns.
 Depreession
 Fasting
 emotions
12/10/2013

Kiran Sharma, Assistant
Professor

25
Measuring BLOOD PRESSURE

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Kiran Sharma, Assistant
Professor

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Professor

27
Sitting position while measuring BP
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Kiran Sharma, Assistant
Professor

28
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Kiran Sharma, Assistant
Professor

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Kiran Sharma, Assistant
Professor

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








Make sure you place the round end of the stethoscope
'under' the blood pressure cuff.
You inflate the sphygmomanometer (blood pressure cuff)
to a little above 180 mm Hg.This collapses the major
arteries to the arm (that's why it is uncomfortable).
Then you slowly release air by gently turning the air
valve, and watch the pressure drop.When you first hear a
sound, that will be the Systolic blood pressure.
The sound you hear is the blood now flowing in the
artery of the arm. This means that the systolic pressure is
now greater than the pressure in the blood pressure cuff.
As you continue to watch the pressure drop, when you no
longer hear any sounds, that will
be the Diastolic blood pressure.
12/10/2013

Kiran Sharma, Assistant
Professor

31


The blood sugar concentration or blood glucose level
is the amount of glucose (sugar) present in the blood of a
human.



The body naturally tightly regulates blood glucose levels
as a part of metabolic homeostasis.



Blood sugar levels outside the normal range may be an
indicator of a medical condition. A persistently high level
is referred to as hyperglycemia; low levels are referred to
as hypoglycemia

BLOOD SUGAR
12/10/2013

Kiran Sharma, Assistant
Professor

32





The international standard way of measuring blood
glucose levels are in terms of a molar concentration,
measured in mmol/L (millimoles per litre; or millimolar,
abbreviated mM).
In the United States, mass concentration is measured in
mg/dL (milligrams per decilitre)
A body's homeostatic mechanism, when operating
normally, restores the blood sugar level to a narrow range
of about 4.4 to 6.1 mmol/L (79.2 to 110 mg/dL) (as
measured by a fasting blood glucose test)

Normal values
12/10/2013

Kiran Sharma, Assistant
Professor

33
High blood sugar (hyperglycemia)
 Long-term hyperglycemia causes many of the long-term
health problems including heart disease, eye, kidney, and
nerve damage.
 The most common cause of hyperglycemia is diabetes.
Low blood sugar(hypoglycemia)
 If blood sugar levels drop too low, a potentially fatal
condition called hypoglycemia develops.
 Symptoms may include lethargy, impaired mental
functioning; irritability; shaking, twitching, weakness in
arm and leg muscles; pale complexion; paranoid or
aggressive mentality and loss of consciousness.

Abnormality in blood sugar levels
12/10/2013

Kiran Sharma, Assistant
Professor

34
Glucose is measured in whole blood, plasma or
serum.
 Historically, blood glucose values were given in
terms of whole blood, but most laboratories now
measure and report plasma or serum glucose levels.


Glucose measurement
12/10/2013

Kiran Sharma, Assistant
Professor

35
Collection of blood in clot tubes for serum chemistry analysis
permits the metabolism of glucose in the sample by blood cells until
separated by centrifugation.
 Red blood cells, for instance, do not require insulin to intake
glucose from the blood. Higher than normal amounts of white or
red blood cell counts can lead to excessive glycolysis in the sample,
with substantial reduction of glucose level if the sample is not
processed quickly. Ambient temperature at which the blood sample
is kept prior to centrifuging and separation of plasma/serum also
affects glucose levels.
 At refrigerator temperatures, glucose remains relatively stable for
several hours in a blood sample. Loss of glucose can be prevented
by using Fluoride tubes (i.e., gray-top) since fluoride inhibits
glycolysis. However, these should only be used when blood will be
transported from one hospital laboratory to another for glucose
measurement. Red-top serum separator tubes also preserve glucose
in samples after being centrifuged isolating the serum from cells.


12/10/2013

Kiran Sharma, Assistant
Professor

36


Two major methods have been used to measure glucose.



The first, still in use in some places, is a chemical method
exploiting the nonspecific reducing property of glucose in a
reaction with an indicator substance that changes color when
reduced. Since other blood compounds also have reducing
properties (e.g., urea, which can be abnormally high in uremic
patients), this technique can produce erroneous readings in
some situations (5 to 15 mg/dL has been reported).



The more recent technique, using enzymes specific to glucose,
is less susceptible to this kind of error. The two most common
employed enzymes are glucoseoxidase and hexokinase.

MEASUREMENT

TECHNIQUES
12/10/2013

Kiran Sharma, Assistant
Professor

37
Measurement techniques
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Kiran Sharma, Assistant
Professor

38
12/10/2013

Kiran Sharma, Assistant
Professor

39
1.
2.
3.
4.
5.
6.
7.
8.

Fasting blood sugar (i.e., glucose) test (FBS)
Two-hr postprandial blood sugar test (2-h PPBS)
Oral glucose tolerance test (OGTT)
Intravenous glucose tolerance test (IVGTT)
Glycosylated hemoglobin (HbA1C)
Self-monitoring of glucose level via patient testing
Random blood sugar (RBS)
Average blood glucose may be estimated by
measuring glycated hemoglobin (HbA1c)

Blood glucose laboratory tests
12/10/2013

Kiran Sharma, Assistant
Professor

40
Clinical correlation
12/10/2013

Kiran Sharma, Assistant
Professor

41


Lung function tests, also called pulmonary function
tests, measure how well your lungs work.



Comparing the measured values for pulmonary function tests
obtained on a patient at any particular point with normal
values derived from population studies.



The percentage of predicted normal is used to grade the
severity of the abnormality.

LUNG FUNCTION TEST
12/10/2013

Kiran Sharma, Assistant
Professor

42


Spirometry - This test measures how much air you can breathe in
and out. It also measures how fast you can blow air out.



Body plethysmography - This test measures how much air is
present in your lungs when you take a deep breath. It also measures
how much air remains in your lungs after you breathe out fully.



Lung diffusion capacity. This test measures how well oxygen passes
from your lungs to your bloodstream.
Lung volumes
Arterial blood gas, shunt fraction measurement, dead space
Airway resistance
Inspiratory/expiratory muscle pressures
Airway reactivity (methacholine/exercise challenge)
Cardiopulmonary exercise test








Pulmonary Function Tests
12/10/2013

Kiran Sharma, Assistant
Professor

43
VT: tidal volume - air inhaled during quiet
breathing
 IRV: inspiratory reserve volume maximal volume inhaled from quiet
breathing
 ERV: expiratory reserve volume - maximal
volume exhaled from quiet breathing
 RV: residual volume - volume remaining
after maximal exhalation


Primary Lung Volumes
12/10/2013

Kiran Sharma, Assistant
Professor

44
TLC: total lung capacity - sum of 4
primary volumes
 VC: vital capacity - volume exhaled from
maximal inspiration to maximal expiration
 FRC: functional residual capacity –
resting, end-expiratory volume
 IC: maximal volume inhaled from FRC


Lung Capacities = Sum of Primary
Lung Volumes
12/10/2013

Kiran Sharma, Assistant
Professor

45


Measurement of the pattern of air movement into and out
of the lungs during controlled ventilatory maneuvers.



You will be asked to breathe through a mouthpiece while
wearing a nose clip. The tester will coach you to take in
as big a breath as possible. You will then blast the air out
as fast as you can until your lungs are completely empty.
You may then be asked to take another deep breath in
again. You will do this three times or more to make sure
the results are accurate.

Spirometry
12/10/2013

Kiran Sharma, Assistant
Professor

46
The Spirogram
12/10/2013

Kiran Sharma, Assistant
Professor

47
A total cholesterol blood test is a blood
test to check the cholesterol and
triglyceride in the blood.
 Total cholesterol testing is necessary to
check the level of cholesterol in the blood.


Cholesterol Test
12/10/2013

Kiran Sharma, Assistant
Professor

48









Cholesterol - Cholesterol is used by the body to produce hormones
and build cells. Excess cholesterol in the blood can clog artery walls
and form plaque. Too much of plaque can increase the risk of heart
disease.
HDL cholesterol - This is good cholesterol that binds with fat in the
blood and helps to remove it from the body.
LDL cholesterol - Known as 'bad cholesterol', LDL cholesterol can
increase the risk of heart disease.
VLDL - This lipoprotein distributes the triglyceride that is created
by the liver. Excessive VLDL can increase the chances of a stroke
and heart disease.
Triglycerides - Triglycerides are fat that is stored in the body as
energy.

Cholesterol attaches itself to a protein and travels through
blood. This package is known as a lipoprotein. A Lipid profile
test is done to check the level of total cholesterol,
triglycerides, HDL cholesterol, and LDL cholesterol.
12/10/2013

Kiran Sharma, Assistant
Professor

49
This is done to screen for a lipid disorder.
 This test is done to check how a person is responding
to treatment for lipid disorders.
 A test like this can determine if you are at a risk of
getting heart disease.
 This test might be done to screen for diseases that
cause high cholesterol in people.


Reasons Why It is Conducted
12/10/2013

Kiran Sharma, Assistant
Professor

50
REPORTABLE RANGE
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Kiran Sharma, Assistant
Professor

51
THANKS
12/10/2013

Kiran Sharma, Assistant
Professor

52

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Health screening services

  • 2.  Health as a state of complete physical, mental and social well-being and not merely the absence of disease or infirmity.  Health is the extent to which an individual or group is able on one hand, to realize aspirations and satisfy needs and on the other hand to change or cope with the environment HEALTH 12/10/2013 Kiran Sharma, Assistant Professor 2
  • 3.  Screening refers to examination of a group of usually asymptomatic individuals to detect those with a high probability of having a given disease, typically by means of an inexpensive diagnostic test.  Monitoring refers to the act of observation and in some cases intervention generally referring to monitoring of a condition after diagnosis to assess and improve outcomes. SCREENING 12/10/2013 Kiran Sharma, Assistant Professor 3
  • 4.  Health screening services are the services provided by the health care professionals to screen the health status of individuals with or without positive sign and symptoms. HEALTH SCREENING SERVICES 12/10/2013 Kiran Sharma, Assistant Professor 4
  • 6.  Early detection can make a difference between relatively simple courses of treatment or lifethreatening complications  Health screenings save lives by early detection of conditions such as hypertension or elevated blood sugar and can help prevent serious diseases  Many cases of disease exist and remain undiagnosed that seriously affect our quality of life. Many of these conditions can be corrected or improved through a simple course of treatment if once it has been discovered by health screening IMPORTANCE OF HEALTH SCREENING 12/10/2013 Kiran Sharma, Assistant Professor 6
  • 7.  One of the additional benefits of a health screen is peace of mind and reassurance.  Health Screening Services contributes to economic burden of the patient by early diagnosis and prevention.  For many people, the diagnosis comes when they make contact with the health system for other reasons for example when admitted or visited to hospital with minor illness or injuries. The longer, people are undiagnosed, the more likely it is the complication of the disease  12/10/2013 Kiran Sharma, Assistant Professor 7
  • 8.  Health screenings are convenient (readily available), affordable (inexpensive), comprehensive, very reliable with few false negatives and flexible. Also it is painless, noninvasive and inexpensive tests. 12/10/2013 Kiran Sharma, Assistant Professor 8
  • 9.  Based on composition of Health screen, it is classified into three types. They are  STANDARD HEALTH SCREEN  PREMIUM HEALTH SCREEN  EXECUTIVE HEALTH SCREEN 12/10/2013 Kiran Sharma, Assistant Professor 9
  • 10.        Consultation with health professional Blood glucose test (marker for diabetes) Blood cholesterol test (total Cholesterol level) Blood pressure measurement and evaluation Body mass indexing (measures height weight ratio) Cardiac risk assessment Comprehensive urine analysis (tests for elevation of protein, blood etc in urine) STANDARD HEALTH SCREEN 12/10/2013 Kiran Sharma, Assistant Professor 10
  • 11.          Consultation with health professional Blood glucose test (marker for diabetes) Blood cholesterol test (total Cholesterol level) Blood pressure measurement and evaluation Body mass indexing (measures height weight ratio) Cardiac risk assessment Pulmonary function tests (lung capacity, and screens for possible disease) Liver function tests (for abnormal liver function) Comprehensive urine analysis (tests for protein, blood in the urine and possible kidney disease infection) PREMIUM HEALTH SCREEN 12/10/2013 Kiran Sharma, Assistant Professor 11
  • 12. Comprehensive one to one consultation  Blood glucose test (marker for diabetes)  Blood cholesterol test (total Cholesterol levels)  Blood pressure measurement and evaluation  Body mass indexing (measures height and weight ratio)  12 lead ECG (measures the electrical conduction of the heart)  Cardiac risk assessment  Renal profile (blood test to check kidney functions)  EXECUTIVE HEALTH SCREEN 12/10/2013 Kiran Sharma, Assistant Professor 12
  • 13. Bone profile (blood test for measure calcium, phosphate)  Full blood count (measures red cells, white cells, hemoglobin etc.)  Ferritin blood test (can detect hereditary conditions such as haemochromatosis)  Pulmonary function tests (lung capacity, and screens for possible disease)  Liver function tests (for abnormal liver function)  Comprehensive urine analysis (tests for protein, blood in the urine and possible kidney disease infection)  Healthy body fat % range  EXECUTIVE HEALTH SCREEN(contd.) 12/10/2013 Kiran Sharma, Assistant Professor 13
  • 14.  Blood pressure (BP), sometimes referred to as arterial blood pressure, is the pressure exerted by circulating blood upon the walls of blood vessels  During each heartbeat, blood pressure varies between a maximum (systolic) and a minimum (diastolic) pressure BLOOD PRESSURE 12/10/2013 Kiran Sharma, Assistant Professor 14
  • 15. Physiology There are many physical factors that influence arterial pressure Amount of blood Viscosity of blood Flow of blood Elasticity of blood vessels Flexibility of walls of blood12/10/2013 Kiran Sharma, Assistant vessels Professor 15
  • 16. During exercise  During tension, fear  During sleeping  Excitement  12/10/2013 Kiran Sharma, Assistant Professor 16
  • 17. PULSE PRESSURE average 40 mm Hg 12/10/2013 Kiran Sharma, Assistant Professor 17
  • 18.  It is alternate expansion and elastic recoil of an artery with each systole.  Pulse rate 70-90 per minute ( same as heart rate) Tachycardia- rapid pulse rate  Bradycardia – slow pulse rate  PULSE 12/10/2013 Kiran Sharma, Assistant Professor 18
  • 20.  HIGH BLOOD PRESSURE (hypertension)   It is a condition in which the arteries have persistently elevated blood pressure. Every time the human heart beats, it pumps blood to the whole body through the arteries. 12/10/2013 Kiran Sharma, Assistant Professor 20
  • 21.       Smoking Obesity or being overweight Being obese/overweight as a child - a research team at the Indiana University School of Medicine found that obese/overweight children are much more likely to suffer from hypertension during adulthood. Diabetes Lack of physical activity Pregnancy Causes of hypertension 12/10/2013 Kiran Sharma, Assistant Professor 21
  • 22.  High levels  Insufficient of salt intake (sodium sensitivity). calcium, potassium, and magnesium consumption  Vitamin D deficiency  High levels of alcohol consumption  Stress  Aging  Medicines such as birth control pills  Genetics and a family history of hypertension  Chronic kidney disease  Adrenal and thyroid problems or tumors 12/10/2013 Kiran Sharma, Assistant Professor 22
  • 23.  Hypertension is classified as either primary (essential) hypertension or secondary hypertension; about 90–95% of cases are categorized as "primary hypertension" which means high blood pressure with no obvious underlying medical cause.[2] The remaining 5–10% of cases (secondary hypertension) are caused by other conditions that affect the kidneys, arteries, heart or endocrine system. 12/10/2013 Kiran Sharma, Assistant Professor 23
  • 24.  Low blood pressure means that your blood pressure is lower than normal Low blood pressure ( hypotension) 12/10/2013 Kiran Sharma, Assistant Professor 24
  • 25. Some of the causes of low blood pressure include:  Not drinking enough fluids (dehydration).  Medicines, such as high blood pressure medicine or other heart medicines.  Health problems such as thyroid disease, severe infection, bleeding in the intestines, or heart problems.  Trauma, such as major bleeding or severe burns.  Depreession  Fasting  emotions 12/10/2013 Kiran Sharma, Assistant Professor 25
  • 26. Measuring BLOOD PRESSURE 12/10/2013 Kiran Sharma, Assistant Professor 26
  • 28. Sitting position while measuring BP 12/10/2013 Kiran Sharma, Assistant Professor 28
  • 31.      Make sure you place the round end of the stethoscope 'under' the blood pressure cuff. You inflate the sphygmomanometer (blood pressure cuff) to a little above 180 mm Hg.This collapses the major arteries to the arm (that's why it is uncomfortable). Then you slowly release air by gently turning the air valve, and watch the pressure drop.When you first hear a sound, that will be the Systolic blood pressure. The sound you hear is the blood now flowing in the artery of the arm. This means that the systolic pressure is now greater than the pressure in the blood pressure cuff. As you continue to watch the pressure drop, when you no longer hear any sounds, that will be the Diastolic blood pressure. 12/10/2013 Kiran Sharma, Assistant Professor 31
  • 32.  The blood sugar concentration or blood glucose level is the amount of glucose (sugar) present in the blood of a human.  The body naturally tightly regulates blood glucose levels as a part of metabolic homeostasis.  Blood sugar levels outside the normal range may be an indicator of a medical condition. A persistently high level is referred to as hyperglycemia; low levels are referred to as hypoglycemia BLOOD SUGAR 12/10/2013 Kiran Sharma, Assistant Professor 32
  • 33.    The international standard way of measuring blood glucose levels are in terms of a molar concentration, measured in mmol/L (millimoles per litre; or millimolar, abbreviated mM). In the United States, mass concentration is measured in mg/dL (milligrams per decilitre) A body's homeostatic mechanism, when operating normally, restores the blood sugar level to a narrow range of about 4.4 to 6.1 mmol/L (79.2 to 110 mg/dL) (as measured by a fasting blood glucose test) Normal values 12/10/2013 Kiran Sharma, Assistant Professor 33
  • 34. High blood sugar (hyperglycemia)  Long-term hyperglycemia causes many of the long-term health problems including heart disease, eye, kidney, and nerve damage.  The most common cause of hyperglycemia is diabetes. Low blood sugar(hypoglycemia)  If blood sugar levels drop too low, a potentially fatal condition called hypoglycemia develops.  Symptoms may include lethargy, impaired mental functioning; irritability; shaking, twitching, weakness in arm and leg muscles; pale complexion; paranoid or aggressive mentality and loss of consciousness. Abnormality in blood sugar levels 12/10/2013 Kiran Sharma, Assistant Professor 34
  • 35. Glucose is measured in whole blood, plasma or serum.  Historically, blood glucose values were given in terms of whole blood, but most laboratories now measure and report plasma or serum glucose levels.  Glucose measurement 12/10/2013 Kiran Sharma, Assistant Professor 35
  • 36. Collection of blood in clot tubes for serum chemistry analysis permits the metabolism of glucose in the sample by blood cells until separated by centrifugation.  Red blood cells, for instance, do not require insulin to intake glucose from the blood. Higher than normal amounts of white or red blood cell counts can lead to excessive glycolysis in the sample, with substantial reduction of glucose level if the sample is not processed quickly. Ambient temperature at which the blood sample is kept prior to centrifuging and separation of plasma/serum also affects glucose levels.  At refrigerator temperatures, glucose remains relatively stable for several hours in a blood sample. Loss of glucose can be prevented by using Fluoride tubes (i.e., gray-top) since fluoride inhibits glycolysis. However, these should only be used when blood will be transported from one hospital laboratory to another for glucose measurement. Red-top serum separator tubes also preserve glucose in samples after being centrifuged isolating the serum from cells.  12/10/2013 Kiran Sharma, Assistant Professor 36
  • 37.  Two major methods have been used to measure glucose.  The first, still in use in some places, is a chemical method exploiting the nonspecific reducing property of glucose in a reaction with an indicator substance that changes color when reduced. Since other blood compounds also have reducing properties (e.g., urea, which can be abnormally high in uremic patients), this technique can produce erroneous readings in some situations (5 to 15 mg/dL has been reported).  The more recent technique, using enzymes specific to glucose, is less susceptible to this kind of error. The two most common employed enzymes are glucoseoxidase and hexokinase. MEASUREMENT TECHNIQUES 12/10/2013 Kiran Sharma, Assistant Professor 37
  • 40. 1. 2. 3. 4. 5. 6. 7. 8. Fasting blood sugar (i.e., glucose) test (FBS) Two-hr postprandial blood sugar test (2-h PPBS) Oral glucose tolerance test (OGTT) Intravenous glucose tolerance test (IVGTT) Glycosylated hemoglobin (HbA1C) Self-monitoring of glucose level via patient testing Random blood sugar (RBS) Average blood glucose may be estimated by measuring glycated hemoglobin (HbA1c) Blood glucose laboratory tests 12/10/2013 Kiran Sharma, Assistant Professor 40
  • 42.  Lung function tests, also called pulmonary function tests, measure how well your lungs work.  Comparing the measured values for pulmonary function tests obtained on a patient at any particular point with normal values derived from population studies.  The percentage of predicted normal is used to grade the severity of the abnormality. LUNG FUNCTION TEST 12/10/2013 Kiran Sharma, Assistant Professor 42
  • 43.  Spirometry - This test measures how much air you can breathe in and out. It also measures how fast you can blow air out.  Body plethysmography - This test measures how much air is present in your lungs when you take a deep breath. It also measures how much air remains in your lungs after you breathe out fully.  Lung diffusion capacity. This test measures how well oxygen passes from your lungs to your bloodstream. Lung volumes Arterial blood gas, shunt fraction measurement, dead space Airway resistance Inspiratory/expiratory muscle pressures Airway reactivity (methacholine/exercise challenge) Cardiopulmonary exercise test       Pulmonary Function Tests 12/10/2013 Kiran Sharma, Assistant Professor 43
  • 44. VT: tidal volume - air inhaled during quiet breathing  IRV: inspiratory reserve volume maximal volume inhaled from quiet breathing  ERV: expiratory reserve volume - maximal volume exhaled from quiet breathing  RV: residual volume - volume remaining after maximal exhalation  Primary Lung Volumes 12/10/2013 Kiran Sharma, Assistant Professor 44
  • 45. TLC: total lung capacity - sum of 4 primary volumes  VC: vital capacity - volume exhaled from maximal inspiration to maximal expiration  FRC: functional residual capacity – resting, end-expiratory volume  IC: maximal volume inhaled from FRC  Lung Capacities = Sum of Primary Lung Volumes 12/10/2013 Kiran Sharma, Assistant Professor 45
  • 46.  Measurement of the pattern of air movement into and out of the lungs during controlled ventilatory maneuvers.  You will be asked to breathe through a mouthpiece while wearing a nose clip. The tester will coach you to take in as big a breath as possible. You will then blast the air out as fast as you can until your lungs are completely empty. You may then be asked to take another deep breath in again. You will do this three times or more to make sure the results are accurate. Spirometry 12/10/2013 Kiran Sharma, Assistant Professor 46
  • 47. The Spirogram 12/10/2013 Kiran Sharma, Assistant Professor 47
  • 48. A total cholesterol blood test is a blood test to check the cholesterol and triglyceride in the blood.  Total cholesterol testing is necessary to check the level of cholesterol in the blood.  Cholesterol Test 12/10/2013 Kiran Sharma, Assistant Professor 48
  • 49.      Cholesterol - Cholesterol is used by the body to produce hormones and build cells. Excess cholesterol in the blood can clog artery walls and form plaque. Too much of plaque can increase the risk of heart disease. HDL cholesterol - This is good cholesterol that binds with fat in the blood and helps to remove it from the body. LDL cholesterol - Known as 'bad cholesterol', LDL cholesterol can increase the risk of heart disease. VLDL - This lipoprotein distributes the triglyceride that is created by the liver. Excessive VLDL can increase the chances of a stroke and heart disease. Triglycerides - Triglycerides are fat that is stored in the body as energy. Cholesterol attaches itself to a protein and travels through blood. This package is known as a lipoprotein. A Lipid profile test is done to check the level of total cholesterol, triglycerides, HDL cholesterol, and LDL cholesterol. 12/10/2013 Kiran Sharma, Assistant Professor 49
  • 50. This is done to screen for a lipid disorder.  This test is done to check how a person is responding to treatment for lipid disorders.  A test like this can determine if you are at a risk of getting heart disease.  This test might be done to screen for diseases that cause high cholesterol in people.  Reasons Why It is Conducted 12/10/2013 Kiran Sharma, Assistant Professor 50